Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,256,0690GC40.0% 41.4 / 10.7 31P133R (CCG→CGG) plsBglycerol‑3‑phosphate 1‑O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (3/15);  new base C (12/0);  total (15/16)
Fisher's exact test for biased strand distribution p-value = 1.05e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGATCCTGACGAGCAGGCAAACGTGGGCCTACGGC‑AGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATC‑CAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTT  >  NC_000913/4255820‑4256319
                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                            
agttctggcgtaGCAGGCAAACGTGGTCCT‑CTGCGAGCCAGACGTGGACGGCCAAAGCGCATCCGCGCCACGCGCGCCTGTTTCTGAGCTATATTTTTATCCCTGCCGTGTACATCCCCCATGCGGCGCAGCGAATCGTACGGCGAGAATCGCAGTAACCTGTCCCGACCTAGCCGCAGTACAGCGAACAATTTCTGTTCTCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCgg                                                                                                                                                                                                                                                             <  1:43107/240‑1 (MQ=255)
              aGGCAAACGTGGGCCTACGGC‑AGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTTACTTC‑GCCTTTTTCTCGCCCCCGCGCGCGACCaaaa                                                                                                                                                                                                                                                >  1:357647/1‑249 (MQ=255)
              aGGCAAACGTGGGCCTACGGC‑AGCCAGACGTTGACGGGAAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGGGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACCAAACa                                                                                                                                                                                                                                               <  1:377917/251‑1 (MQ=255)
                gCAAACGTGGGCCTACGGC‑AGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTTACTTT‑CCCTTTTTTCCGCCCCCGCGCGCGACCCAAccac                                                                                                                                                                                                                                             >  1:9769/1‑248 (MQ=255)
                        gggCCTACGGC‑AGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTTACTTC‑GCCTTTTTTAACCCCCCGCGCGCGCCCAAACACCACCGacac                                                                                                                                                                                                                                     >  1:288780/1‑251 (MQ=255)
                              aCGGC‑AGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCa                                                                                                                                                                                                                                <  1:185791/250‑1 (MQ=255)
                                ggC‑ACCCAGACGTTGACGGGCAAAGTGCGTCCGCCCCACGCGCGCCAGTTTCTGAGCGATAGTTTTACCCGTGCCGTGTTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGTCCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAGGCATGCCCAGCGGCGGGTTCACTTC‑GGCTTTGTCACGCCCCGGCGCGCGACCAAACATCTCCGACACTGGCACCa                                                                                                                                                                                                                             <  1:307786/251‑1 (MQ=255)
                                  c‑AGCCAGACTTTGGCGGGGAAAGTGCATACGCGCCCCGCGCGCCATTTTCGGAGGGATATTTTAATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTTTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTGTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATc                                                                                                                                                                                                                           <  1:186256/251‑1 (MQ=255)
                                                aCGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTC‑CCCCTTTTTACCCCCCCGCCCGCGGCCCAACAACACCCACACCGGCACCaacttccccacccgat                                                                                                                                                                                                              >  1:110873/1‑236 (MQ=255)
                                                 ggggaaaaggccacccgccccccgcgcgCCAGTTTGTGAGTGATAGTTTAACCCGGGCGGTGTCCACCCGCCATACGGCGCAGCGAACCTGACGGCGAGAACCGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATC‑CAGA‑tt                                                                                                                                                                                                           <  2:192356/229‑1 (MQ=255)
                                                       aaGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTTTCCCTTTTTC‑CGCTCCCCCGCGCGACCCAACACCACCCACACTTGCAccctttccacaacccgattttggtt                                                                                                                                                                                                       >  1:5739/1‑228 (MQ=255)
                                                          tGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAACCGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTTAATTC‑GCCTTTTTTACGCCCCCGCGCGCGGCCCAACATCACCCACACCGGCACCACCTGCACACC‑CAGC‑TTTGGGtttct                                                                                                                                                                                                  >  1:47197/1‑248 (MQ=255)
                                                           gcacccccgccgcGCGCCCCATTTTCGGAGCTATAGTTTTACCCGGGCCTTGTCCACCCCCCACACGGCGCAGCGAAACGGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATC‑CAGA‑TTTGGGTTGCt                                                                                                                                                                                                  <  2:110873/243‑1 (MQ=255)
                                                                                  ttcggAGCGATAGTTTTACCCGTGCCGTGTTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATC‑CAGA‑TTTGGGTTGCTACGGTGCa                                                                                                                                                                                          <  1:260536/231‑1 (MQ=255)
                                                                                  ttCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑TCCTTTTTCCCGCCCCCGCGCGCGACCAAACACCCCCGACCCCGGCACCCTCCGCCCATC‑CCGC‑TTTGGGTTGCttccgggcaacatcgggagagcggc                                                                                                                                                                          >  2:260536/1‑227 (MQ=255)
                                                                                      gAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTC‑GCCTTTTTTACGCCCCCGCGCGCGACCACACATCACCCACACTGGCACCCTCTGCACCAC‑CAGAATTTGGGT‑GCTACGGTGCAAAACGAGATAGTCGTGGaa                                                                                                                                                                       >  1:54251/1‑250 (MQ=255)
                                                                                          gATAGTTTTACCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATC‑CAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGc                                                                                                                                                                   <  1:6616/250‑1 (MQ=255)
                                                                                           atatttttacccgtcccgggtcaaTCCGCCATTCGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATC‑CAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCtt                                                                                                                                                                 <  2:156340/228‑1 (MQ=255)
                                                                                                       gtgccgtgTTCACCCGCCATACGGCGCAGCTAAACTGCCGGCGAGAAACTCACAAAACTGTCGCGACCGAGGCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGTTTCACTTC‑GCCTTTTTCACGCCCCGGTGCGCGACCAAACAGCACCGACACTGGCACCATCTGCACATC‑CAGA‑TTTGGGTTGCTACGGTGCATATCGAGATAGTCGTGGAACAGCTTAATAGACtcttc                                                                                                                                                     <  1:287083/251‑1 (MQ=255)
                                                                                                        cgccgtttccctCCGCCATACGGCGCAGCGAAACTGACGGAGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATC‑CAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACtcttct                                                                                                                                                    <  2:117205/240‑1 (MQ=255)
                                                                                                                       ccATCCGGCGCAGCAAAACTGACGGCGAAAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATC‑CAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAg                                                                                                                                     <  2:9769/251‑1 (MQ=255)
                                                                                                                             ggCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGCCCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATC‑CAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAac                                                                                                                               <  1:337003/251‑1 (MQ=255)
                                                                                                                             ggCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTC‑GCCTTTTTTACGCCCCGGCGCGCGGCCAAACAACACCGCCACTGGCACCCTCTGCCCCTC‑CCCA‑TTTTGGGTGCTACGGGGCAAATCGGGATAGTCGGGGGAAAGCCTTATAAACTCCTCTTTCTGCGGGTAAttggggga                                                                                                                                >  2:209215/1‑243 (MQ=255)
                                                                                                                               cgcAGCGAACCTGACGGCGGGAACCGCACAAAACTGTCGCGCCCGAGCCACAGTACAGCGAAAAATGTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCTGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATC‑CAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAacac                                                                                                                             <  1:484322/251‑1 (MQ=255)
                                                                                                                                   gCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATC‑CAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACAcgc                                                                                                                         <  1:462744/251‑1 (MQ=255)
                                                                                                                                           gACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATC‑CAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCccg                                                                                                                   <  2:137209/249‑1 (MQ=255)
                                                                                                                                               gCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCCCTTC‑GCCTTTTTTAAGCCCCGGGGGGCGACCCAACAACACCGACACTGGGACCCTCTGAAAAAC‑CAGA‑TTTGGGGTGCTTCTGGGCAACACGAGATAGTCGGGGAAAAGCTTTATAAACTTTTCTTTTGGCGTGGAATAgggggaaacaacccggcccccgtg                                                                                                              >  1:208378/1‑228 (MQ=255)
                                                                                                                                               gCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGCTCCCTTC‑GCCTTTTTCACCCCCCCGCGCGCGGCCCAACAACACCGACACTGGCACCATCCGCACCTCACCAA‑TTTTGGT‑ACTACCGTGCCAATCGCGATAGTCGTGGAGCAGCTTAATAGAATCTTCTTTCTCCGTGTAATAGGTGAaaacaccccgccccccgcg                                                                                                              >  2:12080/1‑237 (MQ=255)
                                                                                                                                                                       gACCGAGTCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCCTTTTTTCCCCCCCGCGCGCGACCAAACATCACCCACACTCGCACCATCTGCACATC‑CCGA‑CTTGTGTTGCTACGGTGCAAATCGAGATAGTCGTGGGACAGCTTAATAGACTCTTTTTTTGGCGTGTAATAGGGGAACACACGCGGCCCGCCGCGAAATAAAACACAGCGCgggggcg                                                                                       >  2:97360/1‑244 (MQ=255)
                                                                                                                                                                                acaGTACAGCGAACAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTTACTTC‑GCCTTTTTCCCGCCCCCGCGCGCGACCAAACATCACCGACACTGGCCCCATCTGCACATC‑CCGA‑TTTTGGGTGCTTCGGTGCAAATCGAGATAGTCGTGGAAAAGCTTAATAGACTCTTTTTTCTGCGTGTAATAGGTGGAAACACGAGGCCCGCCGTGAATGAAAAAATAACCCGGCAGGAGGGGGCCGTCg                                                                            >  1:357001/1‑251 (MQ=255)
                                                                                                                                                                                  aGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTTACTTC‑GCCTTTTTCACGCCCCCGCGCGCGACCAAACATCACCGACACTGGCACCACCTGCACATC‑CAGA‑TTTTGGGTGCTACGGTGCAAATAGAGATAGTCGTGGGACAGCTTAATAGACTCTTCTTTTGGCGGGTAATAGGTGAAAACACGCCGCCCGCCGTGAATGAAAACAAAACGCGGCAAGAGCGTGCCGTCGAt                                                                          >  1:349973/1‑251 (MQ=255)
                                                                                                                                                                                                                                       ttC‑GCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATC‑CAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGgcgcgc                      >  2:6708/1‑250 (MQ=255)
                                                                                                                                                                                                                                                           ggCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATC‑CAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCttt  >  1:427727/1‑251 (MQ=255)
                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                            
AGATCCTGACGAGCAGGCAAACGTGGGCCTACGGC‑AGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATC‑CAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTT  >  NC_000913/4255820‑4256319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑