Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,401,4590TC43.3% 33.2 / 11.8 30D625G (GAT→GGT) csrDregulator of CsrB and CsrC decay
Reads supporting (aligned to +/- strand):  ref base T (15/2);  new base C (0/13);  total (15/15)
Fisher's exact test for biased strand distribution p-value = 1.75e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGTTACAAATCTCTGCCTGAGTCCAGAAAGGCTTAATGCATCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACTCCCATGAG‑ACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTG‑AGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGC  >  NC_000913/3401212‑3401703
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    
tGTTACAAATCTCTGCCTGAGTCCAGAAAGGCTTAATGCATCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACTCCCATGAG‑ACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTG‑AGGACGCGAAAAAATccc                                                                                                                                                                                                                                                   >  1:10950/1‑251 (MQ=255)
 gTTACAAATCTCTGCCTGAGTCCAGAAAGGCTTAATGCATCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACTCCCATGAG‑ACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATAGTTTTTCACGTTAGTATCAAGTGGCTGTG‑AGGACGCGAAAAAATccc                                                                                                                                                                                                                                                   <  1:330872/250‑1 (MQ=255)
     cAAATCTCTGCCTGAGTCCAGAAAGGCTTAATGCATCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACTCCCATGAG‑ACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTG‑AGGACGCGAAAAAATCCCCTTGc                                                                                                                                                                                                                                              >  1:74097/1‑251 (MQ=255)
                         gAAAGGATTAATGCATCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACTCCCATGAG‑ACGCAGCGCGCATTATTCTACGTGAAAACGGCTTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTG‑AGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTg                                                                                                                                                                                                                          >  2:413659/1‑251 (MQ=255)
                                            cagcaTTCCTCGCCTGTTTGCACCCGCGTAGGGTAATTCATCTGAATATGTGCTGCAACAATCGGGCGAGCAGACAAGCACACTCCCATGAG‑CCGAAGCGCGCATTATTTTACGTGAAAACGGATAAAACGGCAGGTTAAACCGATTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTG‑AGGACGCAAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCg                                                                                                                                                                                                       <  1:161746/251‑1 (MQ=255)
                                                           gTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACTCCCATGAG‑ACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTG‑AGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAacgcacg                                                                                                                                                                                        >  1:89367/1‑251 (MQ=255)
                                                               gCAACCGCGTAAGGTAATTCATCTGCATATGTGCGGCAAAAATCGGGCGAGCAGACAAGCACACTCCCATGAG‑ACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGTTTAACCCGAGTTTCTTTGTAAATATTTTTCCACGTTAGTATCAAGTGGCTGTG‑AGGCCGCGAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCgg                                                                                                                                                                                    <  1:359484/251‑1 (MQ=255)
                                                                       gTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACTCCCATGAG‑ACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTG‑AGGACGCGAAAAAATCCCCTCGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCCCTTCGCGCACGCACGACGTTGGCGTa                                                                                                                                                                             >  2:137960/1‑250 (MQ=255)
                                                                          ggttcattcatCGGCATAGTTCCTGAAACAATCGGGCGAGCAAACAAGCACCCCCCCAAGAG‑CCGCGCCCCGCATTTTTCACCGAGAAAACGGATTAAGCGGCAGGTAAACCCAAGTATTTTTGAAAATATTTTTTCACGTTAGTAACAAGGGGCTGTG‑AGGACTCAAAAAAACCCCCTTTCCCGCCTGTAACACCGCGCTGAATCAGGGTCTTCCACTCGCTTCGCGAACGCGCGCCGGTGTCGTAAAcc                                                                                                                                                                         <  1:92894/248‑1 (MQ=255)
                                                                            gTCATTCATCTGCATATGTGCTGCAACATTCGGGCGAGCAGACAAGCACACCCCCATGAG‑ACGCAGCGCGCATTATTCTACGTAAAAACGGATTAAACGGCAGTTTAACCCGAGTATCTTTTTGAATATTTTTTCACTTTAGTATCAAGTGGCTGTG‑AGGACGCAAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTg                                                                                                                                                                       <  1:377472/251‑1 (MQ=255)
                                                                                ttcatCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACTCCCATGAG‑ACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTG‑AGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTg                                                                                                                                                                   >  1:152658/1‑251 (MQ=255)
                                                                                                          cgggCGACCAGACAAGCACCCCCCCA‑GAGGCCGCAGCGCCCATTATTCTAGGTGAAACCGGATTAACCGGCAGTTTAAACCGGGTACCTTTGGAAATTTTTTTCCACGTTAGTATCAGGTGGTTGTA‑AGGACGCGAAAAAACCCCCTTCCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAg                                                                                                                                         <  1:413659/249‑1 (MQ=255)
                                                                                                              ggAGCAGACATGCACACTCCCATGAG‑ACGCAGCCCGCATTTTTCTACGTAAAAACGGATTAAACGGCAGTTTAACCCGATTATTTTTTTGAATATTTTTTCCCGTTAGTATCAAGTGGCTGTG‑AGGCCGCAAAAAAATCCCCTGGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCtt                                                                                                                                     <  1:9478/250‑1 (MQ=255)
                                                                                                                                   aTGAG‑ACGCGGCCAGCTTTATTCTACGAAAAAACGGATAAAGCGTCAGTGTAACCGGAGTATCTTTGGTAATATTTTTCCACGTAAGTATAAAGGGGTGGTG‑GGGACGCGAAAAACCCCCCTTCACCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTcc                                                                                                                <  2:90904/251‑1 (MQ=255)
                                                                                                                                                                gAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTG‑AGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGcc                                                                                    >  2:55984/1‑251 (MQ=255)
                                                                                                                                                                       gATTAAACGGCAGTTTAAACGGAGTATCTTGGTGAATTTTTTTTCACGTTATTATAAAGGGGCTGGG‑AGGACGCAAAAAAACCCCCTGGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGa                                                                               <  1:91569/249‑1 (MQ=255)
                                                                                                                                                                       gATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTG‑AGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGacg                                                                             >  2:91569/1‑249 (MQ=255)
                                                                                                                                                                         ttAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTG‑AGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATgg                                                                            >  2:139138/1‑250 (MQ=255)
                                                                                                                                                                             acggagggTTAAACCGAGTTTCTTTGTGAATATTTTTTCACTTAATTATCAAGTGGTTGGG‑AGGACGCAAAAAAACCCCCTGCCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTGCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCtt                                                                       <  1:36723/245‑1 (MQ=255)
                                                                                                                                                                              gggcgggtaaacccgattattttggtgAATTTTTTTTCACGTTAGTATCAAGTGGCTGTG‑AGGACGCGAAAAAACCCCCTGGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTg                                                                      <  1:301708/227‑1 (MQ=255)
                                                                                                                                                                                        aaCCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTG‑AGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGGCTGCCACTCGCTTCGCGAACGCACGACGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTcc                                                             >  2:137480/1‑249 (MQ=255)
                                                                                                                                                                                            gAGTATTTTTGTAAATATTTTTCCACGTTAGTATCAGGGGGCTGGG‑AGGACGCAAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACAtt                                                        <  1:139138/251‑1 (MQ=255)
                                                                                                                                                                                                        gAATATTTTTTCACGTTAGTATCAAG‑GGGTGTGGAGGCCGCAAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGa                                             <  1:271037/250‑1 (MQ=255)
                                                                                                                                                                                                              tttttCACGTTAGTATCAAGTGGCTGTG‑AGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACt                                      >  2:146537/1‑251 (MQ=255)
                                                                                                                                                                                                                  ccactttAGTAACAAGGGGCGGTG‑AGGCCGCAAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGt                                   <  2:152658/245‑1 (MQ=255)
                                                                                                                                                                                                                              cAAGTGGCTGTG‑AGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTc                      >  1:233191/1‑251 (MQ=255)
                                                                                                                                                                                                                               aaGTGGCTGTG‑AGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGt                                                                                                                     >  2:341774/1‑155 (MQ=255)
                                                                                                                                                                                                                                  tGGCTGTG‑AGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAAc                  >  1:54517/1‑251 (MQ=255)
                                                                                                                                                                                                                                              acgaaaaaaaaCCCCCTGGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTg       <  2:74097/246‑1 (MQ=255)
                                                                                                                                                                                                                                                   aaaaaaTCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGc  >  1:366890/1‑251 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    
TGTTACAAATCTCTGCCTGAGTCCAGAAAGGCTTAATGCATCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACTCCCATGAG‑ACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTG‑AGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGC  >  NC_000913/3401212‑3401703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑