| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,228,289 | 0 | T | C | 53.8% | ‑0.4 / 11.6 | 13 | intergenic (‑1019/‑693) | yohF/yohP | putative oxidoreductase YohF/putative membrane protein YohP |
| Reads supporting (aligned to +/- strand): ref base T (5/1); new base C (0/7); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.70e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCG‑GGTTTGCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTACCCAGGCGGGCAGTAAC‑CTCCGCCC‑GATTCTTTCCCCAGATAT‑CGAGATGCCAGCCCGCC‑GTTAAGCCAAAAGTA‑CCGTTGGTGTACCAC‑GGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGGTGCGTTCT > NC_000913/2228043‑2228533 | aTTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCG‑GGTTTGCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTACCCAGGCGGGCAGTAAC‑CTCCGCCC‑GATTCTTTcccc < 2:237614/251‑1 (MQ=255) aGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCG‑GGGTTGCTCGCGGTCCCCCCCCCCGGCTTTTA‑ACGTACCCAGGGGGGGAGGAAA‑CTCCCCCC‑CATTGTTTTCCCCCATAT‑CGCGATGCCAGCCCCCCCGTTAGCCCAAAAGTA‑cgctggtgttccc > 1:69702/1‑239 (MQ=255) tCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCG‑GGTTTGCTCCCGGTCCGCCCCCCCGGCTTTAA‑ACCTACCCCCGCGGGCCGGAAC‑CCCCCCCC‑GATTTTTTCCCCCGATAT‑CGGGATGGCAGCCCCCC‑GTTAAACCAAAAATA‑ACCTTGGTGTACCACCGGGCCGG‑CGGACCTGCCGCCgggttg > 1:214399/1‑247 (MQ=255) tttCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCG‑GGGTTGCTCCCGTTCCGCCGCCCGGGCTTTTA‑CCCTACCCAGGGGGGCAGTAACACCCCCCCG‑GATT‑TTTCCCCCGAGATTCG‑GATGCCAACCCGCC‑GGTAAGCCAAAAAGA‑CCGTTTGGGGACCCC‑CGGCCGGTCGTACCTGCGGCCGGATAGTTCAGAGc > 1:89879/1‑250 (MQ=255) aCGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGCGGTTT‑CTCCCGTTCCCCCGCCCGTGCTTTAA‑CCGGACCCCAGCGGGCAGGAAA‑CCCCCCCC‑GCCTCTTTCCCCCGAAAT‑CGAGGTGCCAGCCCCCC‑GGTAAGCCCAAAGTAACCTTTGG‑GGACCCC‑GGGCCCGTCGGAACTGCGGCCGGGTCGGTCAGCGGAAAAGGCCCCCTTTAGCCCTCTGcccgc > 1:59267/1‑248 (MQ=255) ccccccccagtcaaggggggCTCCGCTGCCATCAAGCAATTGGCG‑GTTTGGCCCGCGTCCCGCCCCCCGTGCTTTAC‑CCGTACCCAGGCGGGCAGTACC‑CCCCGCCC‑GATTCTTCCCCAAAATAT‑CGAGATGCCAGCCCGCC‑GTTAAGCCAAAAGTA‑CCGTTGGTGTACCAC‑GGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCa < 1:48090/234‑1 (MQ=255) gctcctcggccaccaagaaatggggg‑ggTTGGCTCGGGTTCCCCCGCCCGGTCTTAAC‑CCGACCCCAGGCGGGAAGAACC‑CTCCGCCG‑GGTTCTTCCCCCAGATAT‑CGAGATGCCAGCCCGCC‑GTTAAGCCAAAAGTA‑CCGTTGGTGTACCAC‑GGGCCGGTCGTACCTGCGGCCGGATCGTGCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGAt < 1:26346/226‑1 (MQ=255) aatttGGGG‑GGTTTGCTCGCGTTCCGCCCCCCGT‑CTTTAAACCCTCCCCAGGCGGG‑AATAAC‑CCCCCCCCCGATTTTTCCCCCAGAAAC‑CGAGTTGCCAGCCCGCC‑GTTAAGCCAAAAGTA‑CCGTTGGTGTACCAC‑GGGCCGGTCGTACCTGCGTCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAg < 1:218855/248‑1 (MQ=255) aatgggCG‑TGTTTGCTCGCGTTCCCCCGCCGGTGTTTTAA‑CCGTCCCCAGGGGGGAAGTACC‑CCCCCCCC‑TATTTTTCCCCCAGATAG‑CGAGGTCCCAGCCCGCC‑GTTAAGCCAAAAGTA‑CCGTTGGTGTACCAC‑GGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGt < 1:264861/247‑1 (MQ=255) gttgatcctgTTCCGCCGCCCGTGCTTTAC‑CCGTCCCCGGGGGGGCA‑TAAC‑CCCCCCCCCGTTTCTTCCCCCAGATAT‑CGAAATGCCAGCCCGCC‑GTTAAGCCAAAAGTA‑CCGTTGGTGTACCAC‑GGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGcc < 1:57315/242‑1 (MQ=255) cgccCGTGCTTAAC‑CCGTACCCAGGCGGGCATTACC‑CCCCGCCC‑GATTCTTCCCCAAGATAT‑CGAGATGCAAGCCCGCC‑GTTAAGCCAAAAGTA‑CCGTTGGTGTACCAC‑GGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCag < 1:168570/251‑1 (MQ=255) cccgggCGGGCAGTACC‑CTCCGCCC‑CATTCTTCCCCCAGATAT‑CAAGATGCCCTCCCGCC‑GTTAAGCCAAAAGTA‑CCGTTGGTGTACCAC‑GGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTCGGCTTCAGCTAGCTGAATGCGCTGTTc < 1:90628/247‑1 (MQ=255) ccccatttcttcCCCCAGATAC‑CGAAGTCCCAGCCCGCC‑GTTAAGCCAAAAGTA‑CCGTTGGTGTACCAC‑GGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGGTGc < 2:16478/245‑1 (MQ=255) aTTCTTTCCCCAGATAT‑AGAGATGCCAGCCCGCC‑GTTAAGCCAAAAGTA‑CCGTTGGTGTACCAC‑GGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGGTGCGTTCt > 2:52690/1‑251 (MQ=255) | ATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCG‑GGTTTGCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTACCCAGGCGGGCAGTAAC‑CTCCGCCC‑GATTCTTTCCCCAGATAT‑CGAGATGCCAGCCCGCC‑GTTAAGCCAAAAGTA‑CCGTTGGTGTACCAC‑GGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGGTGCGTTCT > NC_000913/2228043‑2228533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |