Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	536,549	T→C	intergenic (+63/‑37)	hyi → / → glxR	hydroxypyruvate isomerase/tartronate semialdehyde reductase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	536,549	0	T	C	100.0%	161.1 / NA	49	intergenic (+63/‑37)	hyi/glxR	hydroxypyruvate isomerase/tartronate semialdehyde reductase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/19);  total (30/19)

AAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTAACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCTGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTATGGGTACACCGATGGCCATTAATCTGGCGCGTGCCGGTCATCAATTACATGTCACGACCATTGGACCGGTTGCTGATGAATTACTGTCACTGGGTGCCGTCAGTGTTGAAACTGCTCGCCAGGTAACGGAAGCATCGGACATCATTTTTATTATGGTGCCGGACACACCTCAGGT  >  NC_000913/536304‑536791                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   aaaaTTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAAtt                                                                                                                                                                                                                                               <  2:345742/251‑1 (MQ=255)        gTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGaaa                                                                                                                                                                                                                                        <  2:314143/251‑1 (MQ=255)          cACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGGACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAg                                                                                                                                                                                                                                      <  2:452413/251‑1 (MQ=255)           aCCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGc                                                                                                                                                                                                                                     >  1:215362/1‑251 (MQ=255)                                cGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTCAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGAGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAACACGTGACGCTATTCAGCCAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAAtt                                                                                                                                                                                                                >  1:428970/1‑251 (MQ=255)                                 gCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCACTTTAAGGTAATCGAAAATTCTGACTAAAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAAGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAAtt                                                                                                                                                                                                                >  1:219929/1‑250 (MQ=255)                                                 ggaaccggAGCAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTAt                                                                                                                                                                                               >  1:132621/1‑251 (MQ=255)                                                   caccggAGGAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGGGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAGGCGAAATTTAACATAGGTTAATTATGAAACTGGGATTTATTg                                                                                                                                                                                             >  2:248829/2‑251 (MQ=255)                                                       ggAGAAATTAATTATGATTCTCTCTTTAAGGTAATCGAAAATTCTGCCTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAACTTTAAAAGAGGtt                                                                                                                                                                                                                    >  2:423305/1‑224 (MQ=255)                                                       ggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGtt                                                                                                                                                                                                                    <  1:423305/224‑1 (MQ=255)                                                          gaAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAgg                                                                                                                                                                                      <  1:178069/251‑1 (MQ=255)                                                             attaataaTGCTTCTCTCGTTAAGGTAATCGCAAATTCTGACTCCAACGCTTGGGATGGGGGTGAATATAAACCCCAAACCACCACGGAAGACGGTTTACGCTGGATGGATCCGTACCGTTCAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAACGCGAAATTTAAATGAGGTTAATTATGAAAATGGGATTTATTGGTTTAGGCAt                                                                                                                                                                                   >  2:187053/1‑251 (MQ=255)                                                               taattaTGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCAtt                                                                                                                                                                                  >  1:303328/1‑250 (MQ=255)                                                                          aTCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtagg                                                                                                                                                                      >  1:368826‑M1/1‑240 (MQ=255)                                                                          aTCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGAGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtagg                                                                                                                                                                      >  2:464512‑M1/1‑240 (MQ=255)                                                                                  aaGGTAATCGAAAATTCTGACGACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagac                                                                                                                                                              <  2:347308‑M1/251‑20 (MQ=255)                                                                                            aaaaTTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcct                                                                                                                                                    >  1:371037‑M1/1‑222 (MQ=255)                                                                                              aaTTCTGACTACAACGGTTGGGTTGGGTGTGAATATACCCCCAAAAAAACAACGGAAGCCGCTTTACGCCGGATGGATCCGTACCGTTACAACGTGACGCTACTCAGACAATGCTATTTTAAGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctg                                                                                                                                                   >  1:412398‑M1/1‑220 (MQ=255)                                                                                                 tCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgc                                                                                                                                               >  1:392740‑M1/1‑217 (MQ=255)                                                                                                    gACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcga                                                                                                                                             <  1:464872‑M1/250‑37 (MQ=255)                                                                                                    gACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTAGTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTGAATTATGAAACTGGGATTTATTGGCTTGGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcga                                                                                                                                             <  1:314184‑M1/250‑37 (MQ=255)                                                                                                             ggttgggttgGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgc                                                                                                                                   >  1:17020‑M1/1‑205 (MQ=255)                                                                                                              gttgggttggGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagac                                                                                                                                                              <  1:340309‑M1/223‑20 (MQ=255)                                                                                                              gttgggttggGTGTGAATATAAACCACAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagac                                                                                                                                                              >  2:340309‑M1/1‑204 (MQ=255)                                                                                                                     tgggtGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatc                                                                                                                           >  1:325205‑M1/1‑197 (MQ=255)                                                                                                                      gggtGTGAATCTAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatc                                                                                                                           >  1:119466‑M1/1‑196 (MQ=255)                                                                                                                                ataAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGAGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaa                                                                                                                 <  1:464512‑M1/250‑65 (MQ=255)                                                                                                                                                    gaatccggttTACGCTGTATGGATCCGTACCGTTAAAACGTGACGCTATTCATACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTGAGGCATTAgtagtcgtaggcattagtcattggtgcctgatgcgacgcttgacgcgtcttatcaggccaacaaccggtgccgcagccggcaatt                                                                                            <  2:74695‑M1/247‑86 (MQ=255)                                                                                                                                                                     gatggatCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgat                                                                           <  2:162160‑M1/251‑103 (MQ=255)                                                                                                                                                                      atggatCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAACTTTAAAAGCGGTTAATTATGAAACTGGAATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctccaaccggtgccgcatccggcaattggtgcacaatgcctgatg                                                                          >  1:232967‑M1/1‑148 (MQ=255)                                                                                                                                                                            ccGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgc                                                                    >  1:372780‑M1/1‑142 (MQ=255)                                                                                                                                                                              gTACCGTTAAAACGTGACGCTAGTCAGACAATGCTTTTTTAGGCCGCTAAGGTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAAGTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacattggtgcctgatgcgacgctttacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgctt                                                                  <  2:213627‑M1/251‑112 (MQ=255)                                                                                                                                                                                 ccGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgac                                                               <  1:287611‑M1/251‑115 (MQ=255)                                                                                                                                                                                  cGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacg                                                              <  2:119466‑M1/251‑116 (MQ=255)                                                                                                                                                                                      aaaaCGTGACGCTATTCAGACACTGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTTCGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgcagtcgtaggcattagacatttgtgcatgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatc                                                          >  1:422401‑M1/1‑132 (MQ=255)                                                                                                                                                                                           gggacgcTATTCAGTAAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTGAATTATGAAACTGGGATTTATTGGCTTAGGCTTTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaagtggtgcacaatgcctgatgcgatgcttgtcgcatcttatc                                                     <  2:54537‑M1/248‑125 (MQ=255)                                                                                                                                                                                              aCGCTATTAAGCCAATCCTTATTTAGGCCGCTAAGTTGGCAGGGGATCGCGTTGGCCGAATTCAGGAAACGCGAAATTTAACCGCGGTTACTTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgagacgcttgacgcgtcttatcaggactacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcggtgctggacgcctcttatccgg                                                  >  2:222712‑M1/1‑124 (MQ=255)                                                                                                                                                                                                        gACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgg                                        >  2:260494‑M1/1‑114 (MQ=255)                                                                                                                                                                                                           aaTGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggt                                      >  1:375470‑M1/1‑111 (MQ=255)                                                                                                                                                                                                                 ttgttaggCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgccggatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccgga                                <  2:368826‑M1/247‑146 (MQ=255)                                                                                                                                                                                                                   tttAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATGCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatc                              <  2:371037‑M1/250‑148 (MQ=255)                                                                                                                                                                                                                        gCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtagg                        >  1:162984‑M1/1‑98 (MQ=255)                                                                                                                                                                                                                               aGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggat                  >  1:151354‑M1/1‑91 (MQ=255)                                                                                                                                                                                                                               aGTTGGCAGGGGATCGTGTTCTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGCTAATTATGATACTGGCGTTTATTGGCTTAGGCATTCgtagtcgaaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcaccatgcctgatgcgatgcttgacgcctcttagcaggcctacaatgggtaccggaacggtaggccggat                  >  2:171523‑M1/1‑91 (MQ=255)                                                                                                                                                                                                                                   ggCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggc             <  1:267266‑M1/251‑165 (MQ=255)                                                                                                                                                                                                                                    gCAGGGGATCGTGTTGTCCGCATTCAGGAAAAGCGCCATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcg            >  2:234233‑M1/1‑86 (MQ=255)                                                                                                                                                                                                                                      tgtggatcGTGTTGTCCGAATTAAGGCAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCAGTAgaagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctaccaccggcgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcccacaatgagtaccggatcggtaggccggataagacttt          >  2:60257‑M1/4‑84 (MQ=255)                                                                                                                                                                                                                                      aGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtt          >  2:171740‑M1/1‑84 (MQ=255)                                                                                                                                                                                                                                               tgttgtCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgc  >  2:158893‑M1/1‑75 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   AAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTAACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCTGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTATGGGTACACCGATGGCCATTAATCTGGCGCGTGCCGGTCATCAATTACATGTCACGACCATTGGACCGGTTGCTGATGAATTACTGTCACTGGGTGCCGTCAGTGTTGAAACTGCTCGCCAGGTAACGGAAGCATCGGACATCATTTTTATTATGGTGCCGGACACACCTCAGGT  >  NC_000913/536304‑536791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑