Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,558,907	T→A	K207M (AAG→ATG)	iadA ←	isoaspartyl dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,558,907	0	T	A	96.0%	211.2 / NA	78	K207M (AAG→ATG)	iadA	isoaspartyl dipeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/3);  new base A (36/37);  total (37/41)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCCGTCGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCT  >  NC_000913/4558668‑4559152                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      tGCCGTCGGAGCGGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGGCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTc                                                                                                                                                                                                                                             <  1:178846/250‑1 (MQ=255)       cGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTGCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt                                                                                                                                                                                                                                       <  2:19532/250‑1 (MQ=255)                    gatgcgagacAGCGGACCACCCGCATGAACGGCGTGGGAAAAAGCTTCGGCAGGGGCGACCGGATCGTCAATGATGCTGGTGATAGCCATGGTGCCGCCTTTGCGCGCGAACTACCGCGCCTGCTCAAACAACGGTACGT‑TCCGGTTAACGAGGGTCGG‑CAGCAGCTTGCAGCTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCAGTTTACTATCGCCCAtgtagtatacgg                                                                                                                                                                                                                            >  2:383252/10‑239 (MQ=255)                    gACGCGTGCCAGAGGACTCCCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTGTCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGa                                                                                                                                                                                                                         <  2:368204/251‑1 (MQ=255)                        cgTGCCAGCGGAATACCCGACTGACCGGCGCGGGCACTTCCTTCGGCAGGACCGACCGGCACGTCAATGCTCCTGGCGATATCGATGGTGACGCCTTTGCGCGCGACCTCCAGCGCCTGCTCACACCAAGGTACGT‑TGCGGTTACCGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCACTTTTACTGTCGCCCATGTGGAACACGGTGACGcc                                                                                                                                                                                                                     >  2:302633/1‑251 (MQ=255)                           gCCAGCGGAATCCCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg                                                                                                                                                                                                                  <  1:310310/251‑1 (MQ=255)                           gCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg                                                                                                                                                                                                                  >  1:439855/1‑251 (MQ=255)                              gggggaatggccGCCTGAGCGTCGCGGGCAATACCTTCGGCATGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCGTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACTT‑TGCGGTTAACGTGGGTGGG‑CAGCAGCTTGCTGATCGGCACATCGCAGGTTTCCAGCAGGTCATAAATTGGCTGTAGCGCCCTTTTACTGTCGCCCTTGTGGAACACGGTGACGCCAGGttt                                                                                                                                                                                                               <  1:205071/241‑1 (MQ=255)                                cggaacgcccGCCTGAACGGCGCGGGCAATCCCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTTGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAc                                                                                                                                                                                                             <  2:372572/244‑1 (MQ=255)                                          gccggAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGAGGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaa                                                                                                                                                                                                   <  2:109654/247‑1 (MQ=255)                                            cTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAc                                                                                                                                                                                                 >  2:124374/1‑251 (MQ=255)                                                 cGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCa                                                                                                                                                                                            <  1:125913/251‑1 (MQ=255)                                                  ggcgcgctcAATACCTTCGGCAGGGGCGACTGGTTCGTCACTGCTGCTGGCGATCTAGATGGCGCCGCCTTTGCGCGCGAACTCCACCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCCGATCGGCACATCGCAGTTTTCCCGCAGGTCATAAATACGCTGTCACGCCATTTTACTCTCGCCCATGTGGAACACGGTGCCGCTAGGTTTACCGCCGAGCAAACCGCCa                                                                                                                                                                                            >  2:168576/9‑250 (MQ=255)                                                  ggCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGACAATGCTGCTGGTGATATCGATGGAGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCa                                                                                                                                                                                            >  1:202708/1‑250 (MQ=255)                                                        ggCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCggg                                                                                                                                                                                     <  1:423814/251‑1 (MQ=255)                                                        ggCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGAAGCCAGGTTTACCGCCGAGCAAACCGCCAACGCggg                                                                                                                                                                                     >  2:129590/1‑251 (MQ=255)                                                            atacgttgggccggagCGACCGGTTCGTCAATGCTGCTGGTGATCTCTAGGGTGCCGCCTTTGCGCGCGACCTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAGCACGTTGACGCAAGTTTTACCGCCGAGCAAACCGCCAACGCGGGATTc                                                                                                                                                                                 <  2:86526/236‑1 (MQ=255)                                                              cccctCGGCAGGGGCGCCCGGGCCGTGAAGGCTGCTGGTGATATCGAGGGTGCCGCCTGTGCGCGCGAACCCCCGGGCCTGCTCAAACAACGGTACGTGTG‑GGTTAACGTGGGTCGG‑CAGCAGCTTGCTGACCGGCACATCGCAGTTTCCCAGCAGGTCAAAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGATCACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCt                                                                                                                                                                                <  2:365245/246‑1 (MQ=255)                                                                      cAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCat                                                                                                                                                                        >  1:26469/1‑250 (MQ=255)                                                                          ggCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCCACGCGGGATTCTGCCGCCCTATTgg                                                                                                                                                                   >  2:376301/1‑251 (MQ=255)                                                                            cGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGcc                                                                                                                                                                 >  1:148695/1‑251 (MQ=255)                                                                             gACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGcc                                                                                                                                                                 >  1:332323/1‑250 (MQ=255)                                                                             gACCGGATCGTCAATGCTGCTGGTGATATCGCAGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCCTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGGTTACCGCCGAGCAAACCGCCACCGCGGGATTCGGCCGCCatctgggcc                                                                                                                                                                 >  2:132997/1‑243 (MQ=255)                                                                               ccGGTTCGTCAATGCTGCTGGTGATATCGAGGGTGCCGCTTTTGCGCGCGAACTCCAGCGCCTGCTCAACCAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTAATAAATAGGCTGTACCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAgg                                                                                                                                                              <  1:310892/251‑1 (MQ=255)                                                                                 ggTTCGTCAATGCTGCTGTGGATATCGATGGGGCCGCCTTTGCGCGCGAACGCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACAGCGCAGTTTTCCAGCAGGTCATAAATAGGCTTTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTg                                                                                                                                                            <  1:267773/251‑1 (MQ=255)                                                                                     cGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATa                                                                                                                                                         >  1:180076/1‑250 (MQ=255)                                                                                        cAATGCTGCTGGTGATATCGATGTTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑AAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCAAGGTGATaaaa                                                                                                                                                      <  1:13920/250‑2 (MQ=255)                                                                                            gctgctGTTGATATCGATGTTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAGGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCg                                                                                                                                                 <  1:416189/251‑1 (MQ=255)                                                                                            gctgcgggggatggcagtggggcacccTTTGCGCGCAACCCCCAGCTCCTGCTCAAACACCGTTACGT‑TGCGGTTAACG‑GGGTGGGGCACCACTTTGCTGATGGGAACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGCCGCCAATGTGGAACACGGGGACGCCAGTTTTCCCGCGGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTcc                                                                                                                                                  <  2:385019/225‑1 (MQ=255)                                                                                             ctgctgGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGTTTCTGCCGCCATATTGGCCCGGTGATAAACGTCCgg                                                                                                                                                >  2:151852/1‑251 (MQ=255)                                                                                                ggggTGATATCGAGGGGGCCGTCTTTGCGCGCGAACTCCAGCGCCTTCTCAAACAACGGTAGGT‑GGCGGTAAAGGGGGGCCGG‑GAGAAGCTTGCTGGTCGGCACATCGCAGTTTTCCAGCAGGTCAAAAATAGGCGGTAACGCCATTTGACTGTCGCCCGTGTGGAACCCGTGGACGCCAGGTTTCCCGCCGAGAAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGc                                                                                                                                             <  2:101952/249‑1 (MQ=255)                                                                                                ctgGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGc                                                                                                                                             >  1:30762/1‑251 (MQ=255)                                                                                                 tgGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCg                                                                                                                                            >  1:398657/1‑251 (MQ=255)                                                                                                   gggaaaggtagggTGCCGCCTTTGCGGGCGAACCCCAGCGCCTGCGCAAACACCGTGACTT‑TGCGGTTAACGTGGGGCGG‑CATCAGCGTGTTGTTCGGCACTTCGCAGTTTTCCAGCAGGTCAGAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGACCACGGTGACGCAAGGTTGCCCGCCGAGCAAACCGCCAACGCGTGCTCCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGc                                                                                                                                          <  2:450094/240‑1 (MQ=255)                                                                                                    ggaatacGATGGGGCCTCATTTGCGGGCGAACTCCAGGGCCGGCTAAAACAGCGGTAGGT‑TGCGGTAACCGGGGGTCGG‑AAGCAGCTTGCGGATCGGCACATCGCAGTTTTCCAGAAGGTCAAAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACAGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCa                                                                                                                                         <  2:91936/245‑1 (MQ=255)                                                                                                     gATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAGCGCCATTTTACGGTCGCCCATGTGAAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAg                                                                                                                                        <  1:459829/251‑1 (MQ=255)                                                                                                       tatCGATGATGACGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACCACGGTACGT‑TGCGGCTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAACTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCCGGTTTACCGCCGAGCAACCCGCCAACGCGGGCTTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTCCGGCAGa                                                                                                                                       >  2:180311/1‑250 (MQ=255)                                                                                                         tCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACg                                                                                                                                    >  1:20136/1‑251 (MQ=255)                                                                                                          cGATGGTGCCGCCTTTGCGCGCGAACGCCGGCGCCGGGTCAAACAACGGTACGT‑TGCGGTTAACGGGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTAAAAAAGAGGCTGTAACGCCATTTTACGGTCGCCCATGGGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCAAAGGCGGGATTCTGCCGCCATATTGGACAGGTGATAAACGTCCGGTGCGGCAGAACg                                                                                                                                    <  1:105619/250‑1 (MQ=255)                                                                                                             gggTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGGGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGa                                                                                                                                <  1:48922/250‑1 (MQ=255)                                                                                                              ggTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAAGGTGGGTCGG‑CAGCATCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAAGAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGAt                                                                                                                               <  1:60043/251‑1 (MQ=255)                                                                                                                tGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGCCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATc                                                                                                                              >  1:300206/1‑250 (MQ=255)                                                                                                                  ccgccTTTGCGAACGCACTCCAGAGCCTGCTCAAACAACGGTACAT‑TGCGGTTAAGGTGGGTCGG‑CCGACGCTTACGGATCGGAACATCGCAGTTTTCCAGCAGGTCATAAATAGACTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTAAAGCAAGGTTTACCACTGATAACACCGCCAACGCGGGTTTATGCAGCCATATTGACCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATc                                                                                                                              >  2:61928/1‑248 (MQ=255)                                                                                                                        ttGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGA‑TGCGATACACGTGGGTCGG‑CAGCCGCTTGCTGATCGGCACATTGAAGTTTACCAGCAGGTCATAAATAGGCAGTCACGACATTTTACTGTCGCCCATGTGGAACCCGGAGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGAGATCAACGTCCGGGGCGGCAGAACGGTGCACAGAGATGgc                                                                                                                     >  1:294266/1‑251 (MQ=255)                                                                                                                                   cTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCCTATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGc                                                                                                          >  2:405339/1‑251 (MQ=255)                                                                                                                                     ccatcgccggcacaaaaaccggcaCGT‑TGCGGTGAACGTGGGGCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGGCATAAAGAGGCTGTAACGCCATTTTACTGTCCCCCAGGGGGAACACGGTGACGCAAGTTTTCCCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCa                                                                                                        <  2:344452/228‑1 (MQ=255)                                                                                                                                     ccAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGccc                                                                                                        >  2:247708/1‑250 (MQ=255)                                                                                                                                        gcgcCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATc                                                                                                     >  1:168681/1‑251 (MQ=255)                                                                                                                                        gcgcCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATc                                                                                                     <  1:129590/251‑1 (MQ=255)                                                                                                                                                 cAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CCGCAGCTTGCTGATCGGCACATCGCCGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACAcgatcg                                                                                            >  2:176611/1‑251 (MQ=255)                                                                                                                                                      aacGGTACGA‑TGAGGTTACCGTGGGTCGG‑AAGCTGCTTGCTGATCGGCAAATCGCAGTTTTCCAGAAGGTCATCAATAGGCTGTACAGCCATTTTTCTGTAGCCCATGTGGAACACGGTGACGCCAGGTTTACCGACGAGCCAACCGCCAACGCGGGCTTCTGCCGCCCTATTGGCCAGGTGATAAACGTACGGAGCGGCAGAACGGGGATCAGAGATGGCGAATTTAACGCCCATCATACGATCGGTAAt                                                                                       >  2:216125/1‑251 (MQ=255)                                                                                                                                                       acGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACCTCGCAGTTTTCCAGCAGGACATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGGGATCAGAGATGGCACATTTCAAGCCAATCACACGATCGATAAt                                                                                       >  1:431269/1‑250 (MQ=255)                                                                                                                                                       acGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGGGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATc                                                                                      >  2:314191/1‑251 (MQ=255)                                                                                                                                                        cGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTTCTGATCGGCACATCGCAGTTTTCAAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCCTGTGGAGCACGGTGACGCCAGGGTTACCGCCGAGCAAACCGCCAACTCGGGTGTCTGCCGCCATATTGGCCAGGCGATATGCGTCCGGTACGGCGGCACGGTGATCATAGCTGGCGCAGTTCACGCCAATCACACGATCGATATTCg                                                                                     >  2:87080/1‑251 (MQ=255)                                                                                                                                                          gTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGCACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGcc                                                                                   >  2:232702/1‑251 (MQ=255)                                                                                                                                                           caCGT‑GGCGGTTAGCGGGGTTCGG‑CAGAGGCGTGCGGAGCGGCACCTCGCAGTTTCCAAGCAGTAAATAAAGAGGCGGAACCGCCATTTTACGGTCGCCCGTGTGGAACAGGGTGACGCCAGTTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCa                                                                                  <  2:384817/250‑1 (MQ=255)                                                                                                                                                             cGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTGGCTGATCGGCCCTGCTCAGTTTTCCGGCAGGTCATAAATAGGCTGTAACGCCATTTGACGGTCGCCAATGTGAAACACGTGGACGCCAGTTTGACCGCCGAGCAACCCGCCAAGGCGGGATTCTGCCGCCAGATTGTGCAGGTGTGAAAGGTCCGGGGCGGCAGAACGGTGATCAGAGAGGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACg                                                                                <  1:292944/251‑1 (MQ=255)                                                                                                                                                              ggtggCGGTAAACGTGGGTGGG‑CGGCGGTTGGCGGATCGGCACATCGCAGTTTTCCAGCGGGTCATAAAGAGGCTGTACCCCCATTTTACGGTCGCCCATGGGGAACACGGTGGCGCCAGTTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACg                                                                                <  2:148695/247‑1 (MQ=255)                                                                                                                                                                 tGCGGTTACCGGGGGCCGG‑CAGCAGCTTGCGGATCGGCCCACAGCAGTTTTCCCGCCGGTCATAAATAGGCTGTAACGCCATTTACCTGCCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACGGCCAACGCGGGATTCGGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTc                                                                              <  1:325598/250‑1 (MQ=255)                                                                                                                                                                    ggTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCtttt                                                                          <  1:159927/251‑1 (MQ=255)                                                                                                                                                                              ggTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAAc                                                                <  1:405339/251‑1 (MQ=255)                                                                                                                                                                                         agcTTGCTGATCGGCCCATCGCAGTTTCCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTCCCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGt                                                      <  1:27840/251‑1 (MQ=255)                                                                                                                                                                                           cTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGc                                                    >  1:335737/1‑251 (MQ=255)                                                                                                                                                                                            ttGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCg                                                   >  1:168577/1‑251 (MQ=255)                                                                                                                                                                                              gCTGATCGGCACATCGCAGTTTCCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCggg                                                 <  1:151852/251‑1 (MQ=255)                                                                                                                                                                                                 gATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGGGCGGCCGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACCCGATCGCTAATCGCCACGTGTTTTTCCACGGAACCCGTAATGGTGCGGGa                                                >  2:364521/1‑249 (MQ=255)                                                                                                                                                                                                   ccGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTACCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGTTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAAGCGCCACGTCTTTTTCCACTGAACCCGGAATGGTGCGGGAAggg                                            <  1:180311/250‑1 (MQ=255)                                                                                                                                                                                                             gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAg                                  <  1:124374/251‑1 (MQ=255)                                                                                                                                                                                                                     ccAGCGGGTCATAAATAGGCTGTAACGCCTTTTTCCTGTCGCCCATGTGGAACACGTTGACGCCGGGTTTACCTCCGAGCAAACCGCAAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTc                          <  1:116671/251‑1 (MQ=255)                                                                                                                                                                                                                       agcagGTTATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTGTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAg                        <  1:243066/251‑1 (MQ=255)                                                                                                                                                                                                                              cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAg                 <  1:247708/251‑1 (MQ=255)                                                                                                                                                                                                                               aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAgg                >  2:109194/1‑251 (MQ=255)                                                                                                                                                                                                                                tAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGAGCGGGCAGGGACACGATAAGCGCCGGTCAGCATCCTGgc               >  2:369297/1‑251 (MQ=255)                                                                                                                                                                                                                                       gCTGTAACGCCATTTTACTGTCGCCCATGTGGCACACGGTGACGCCAGGTTTACAGCCGAGCAACCCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATACTCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGCAGGGCCATGATAAGCGCAGGTCAGCATCCAGGCGCTGagg        >  2:177413/1‑249 (MQ=255)                                                                                                                                                                                                                                       gCTGTAACGCCATTTTACTGTCGCCCATGTGGAACCCGGTGCCGCCAGTTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGATg        <  1:394138/251‑1 (MQ=255)                                                                                                                                                                                                                                       gCTGTAACCCCTTTTTACTGGCCCCCTGGTGGACCCCGGGTACGCCAGGTTTACCGCCGAGCAAACCGCCAACGGGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGTACATGATAAGCGCCGTTCATCATCCAGGCGCTGATg        <  2:439855/251‑1 (MQ=255)                                                                                                                                                                                                                                             aCGCCTTTTTCCTTTCGCCCAGGTAGAACACGGTAGCGCCAGGTTTACCGCCGAGCAACCCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCTGTGCGGCAGAACGGTGATCAGAGATGTCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCATGCGGTGATGCcttct  <  2:233167/251‑1 (MQ=255)                                                                                                                                                                                                                                             aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGCCATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCcttct  >  2:130896/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      TGCCGTCGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGT‑TGCGGTTAACGTGGGTCGG‑CAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCT  >  NC_000913/4558668‑4559152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑