Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	536,173	T→C	L155P (CTC→CCC)	hyi →	hydroxypyruvate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	536,173	0	T	C	100.0%	97.6 / NA	31	L155P (CTC→CCC)	hyi	hydroxypyruvate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/12);  total (19/12)

TCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCG‑CATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTAC  >  NC_000913/535958‑536408                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             tCGGGATGGCGTAAAAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGACTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGACCAGATTCACGCAACGCTTGTAGAAACCCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTAATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATtgatg                                                                                                                                                                                                           >  2:320171/1‑251 (MQ=255)                                gCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTTAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTa                                                                                                                                                                            <  1:320171/251‑1 (MQ=255)                                 cccGTGCGCTGGGTAATAAAAAAATTAAATGTCTGGTCGGTAAACCGCCGGCTGGTTTCAGCAGTGAACCGACTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATCTGCTGATGAAAGAAGATATTTTATTACTGCTTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGATGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAgcat                                                                                                                                                                           >  2:464872/1‑248 (MQ=255)                                          tGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATgttggtt                                                                                                                                                                                                  <  1:71177/219‑1 (MQ=255)                                          tGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATgttggtt                                                                                                                                                                                                  >  2:71177/1‑219 (MQ=255)                                               aataaAAAAATTAACGGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGGTTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCatat                                                                                                                                                             <  2:469571/251‑1 (MQ=255)                                                    aaaaaTTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGc                                                                                                                                                        >  2:126407/1‑251 (MQ=255)                                                                          aaaaCGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCa                                                                                                                                   >  1:267029/1‑250 (MQ=255)                                                                                ccGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAGCCATTTTGATATGCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAg                                                                                                                                                         <  1:252844/222‑1 (MQ=255)                                                                                ccGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGCTATTCCTGGTTTCCATCCCAACGGAACTCGGCCGGCGCTGAAATTGATTGTTGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACCTTTATCATATGCAg                                                                                                                                                         >  2:252844/1‑222 (MQ=255)                                                                                                 tGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCCGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGAt                                                                                                            >  2:178069/1‑250 (MQ=255)                                                                                                                                     ttATGCCGCGAATATGCTGATGAAAGAAGATATTTTCTTACTGCTTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAatt                                                                                                                                                                                                                   >  1:53513/1‑111 (MQ=255)                                                                                                                                     ttATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAatt                                                                                                                                                                                                                   <  2:53513/111‑1 (MQ=255)                                                                                                                                                    gCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCG‑CATCGCGGCgaaccggga                                                        <  2:267029/250‑1 (MQ=255)                                                                                                                                                     cTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCG‑CATCGCGGCgaaccgggaa                                                       >  1:400239/1‑250 (MQ=255)                                                                                                                                                      tgatgaAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCG‑CATcgcg                                                                   <  1:31214/237‑1 (MQ=255)                                                                                                                                                      tgatgaAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCG‑CATcgcg                                                                   >  2:31214/1‑237 (MQ=255)                                                                                                                                                       gatgaAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAAGTGGTCACCTGCAAATTGCCGATAATCCG‑CATCGCGGCgaaccgggaa                                                       <  2:400239/248‑1 (MQ=255)                                                                                                                                                              gaagaTATTTTATTACTGATTGAACCTATTAACAATTTTGATATGCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTTAAATTGATTGATTGTGTTGGTTGCTGCAATTTGAAAATTCAGTTTGACATTTATCATATGCAGAGGATGGAAGGTGACTTAACCAACACCATGACTCAGAGGGCTGATAGAAATGGTCACGTGCAACTTGCTGCTAATCCG‑CATCGCGGAGAAGCGGGAAACGGAGAaat                                             >  2:267266/1‑251 (MQ=255)                                                                                                                                                                         attaCTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCAATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGGTGCTGCAATTTGAACATTCAGTATGACCTTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAACTTGGACACCTGCAAATTGCCGATAATCCG‑CATCGAGGCGACCCGGGAAACGGAGAAATTAATTATGAtt                                  >  1:408937/1‑251 (MQ=255)                                                                                                                                                                         attaCTGATTGAACCTATTAACCATCTTGATCTACCTGGTTTCCTTCCCACCAGAACTCCGCAGGAGCTGAAATTGATTGATGCTGTTAGTTAATGAAATTTGAAAATTCAGTATGACATTTATCATATGAAGCGGAAGGATGGTGAATTAACCAAAACCATGATTCAGTGGGCTGATAAAATTGGGCACGTGCAAATACCCGATAATCTGACA‑CGCGGCGGACAGGGACCCGGAGAAATTAATTATGact                                  >  2:35124/1‑249 (MQ=255)                                                                                                                                                                            aCTGATTGAACCTATTAACCAGTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCG‑CATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTAtc                               <  1:28803/251‑1 (MQ=255)                                                                                                                                                                                 ttGAACCTATTAACCATTTTGATATTCCTGGTTTCCCTCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCG‑CATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCttt                          >  1:162160/1‑251 (MQ=255)                                                                                                                                                                                     aCCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCG‑CATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGAt                                   >  1:390275/1‑238 (MQ=255)                                                                                                                                                                                     aCCTATTAACCATGTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCG‑CATCGCGGCGATCCGGGAACCGGAGAAATTAATTATGAt                                   <  2:390275/238‑1 (MQ=255)                                                                                                                                                                                        tATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACt                                                                                                                        <  1:354124/151‑1 (MQ=255)                                                                                                                                                                                        tATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACt                                                                                                                        >  2:354124/1‑151 (MQ=255)                                                                                                                                                                                          ttAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGACAATTCAGTATGACATTTATCATATGCAGCGGCTGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCG‑CATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATc                 >  1:54537/1‑251 (MQ=255)                                                                                                                                                                                            aaCCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCG‑CATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCg                >  1:213627/1‑250 (MQ=255)                                                                                                                                                                                                    tGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCG‑CATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCt        <  1:344854/250‑1 (MQ=255)                                                                                                                                                                                                         ttCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCCATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCG‑CATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGATAATCGAAAATTCTGACTAc  >  1:28228/1‑251 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             TCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCG‑CATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTAC  >  NC_000913/535958‑536408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑