Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,312,215	A→G	T259T (ACT→ACC)	alsR ←	DNA‑binding transcriptional repressor AlsR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,312,215	0	A	G	100.0%	75.2 / NA	25	T259T (ACT→ACC)	alsR	DNA‑binding transcriptional repressor AlsR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/13);  total (12/13)

GGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACAT  >  NC_000913/4311968‑4312451                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              ccattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAaggttg                                                                                                                                                                                                                                           <  2:123820‑M2/162‑2 (MQ=255)    ttttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAggttggtt                                                                                                                                                                                                                                        >  1:462536‑M2/87‑251 (MQ=255)             gggtaattccctgatattacgggcactatgtattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTGATTGCAGCGCGCCTGGTGAGAAGAAATCAACAAGTGCGCCGGTGTGTTCCATATTAAGAGTAGCTTGTTCAATTGTGAGCTGGGCGACAGAGACAAAAAAAGCTTCCAGCAAGGTT‑GTTGGTAATATTc                                                                                                                                                                                                                              <  2:410384‑M2/174‑1 (MQ=255)                    tccctgatattacgggcactctttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTT‑GTAATATTCTTGCCg                                                                                                                                                                                                                        >  2:242138‑M2/71‑250 (MQ=255)                                 gggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCt                                                                                                                                                                                                          >  1:386875‑M2/58‑251 (MQ=255)                                     cctatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCtaata                                                                                                                                                                                                      <  1:62335‑M2/198‑1 (MQ=255)                                        atttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACctaatat                                                                                                                                                                                                     <  2:356397‑M2/199‑2 (MQ=255)                                                   actctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCggg                                                                                                                                                                                        >  1:330683‑M2/40‑251 (MQ=255)                                                                                        gaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCt                                                                                                                                                    <  1:119401‑M2/248‑1 (MQ=255)                                                                                         aGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTAt                                                                                                                                                  <  2:152964‑M2/250‑1 (MQ=255)                                                                                                      agTAACTCCGCGATGGCGGAATTACCTTTTTCTTAGTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTa                                                                                                                                      <  1:272894/250‑1 (MQ=255)                                                                                                            cccGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCTGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGAAAAAAAAAGCGTCCAGCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATTTGATCGGCCAGTTTCGCTTGCGGTGAATGGTAGCTATg                                                                                                                                <  1:363514/250‑1 (MQ=255)                                                                                                                                    tCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAg                                                                                                                                     >  1:24002/1‑222 (MQ=255)                                                                                                                                    tCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGGTTCGCTATCGGTGAATGGTAg                                                                                                                                     <  2:24002/222‑1 (MQ=255)                                                                                                                                    tCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATGGCGCCGGTTTTGTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGGT‑GTAATATTCTTGCCGAGGCATGACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCTAGGTTCGCTATCGGTGAATGGTAGCTATGGGTTATGCAAATAATCTTTGccc                                                                                                         <  2:226453/250‑1 (MQ=255)                                                                                                                                                      ggTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTc                                                                                      >  1:196635/1‑251 (MQ=255)                                                                                                                                                         gagaAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGTTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGGAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTAc                                                                                   <  1:444600/251‑1 (MQ=255)                                                                                                                                                           gaagaaATAAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTT‑GTAATATTCTTGCCGCGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACgg                                                                                 >  2:269481/1‑251 (MQ=255)                                                                                                                                                             agaaATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACTGcc                                                                               >  1:279974/1‑251 (MQ=255)                                                                                                                                                                        aTTGCGCCGGTTTGTTCCATATTAAGATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGCT‑GTAATAGTCTTGCCGAGGCATTACGACCTAATATCGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTGCGCGATCGGTGAATGGTAGCTATGGGTTATACAAAGAATCTTGGCCCCGTTCTTTTGTGCCAGTACTACGTCCGCTGTTACATc                                                                    <  2:237093/251‑1 (MQ=255)                                                                                                                                                                              ccGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAACAAGCGTCCAGCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCAAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACttgt                                                              >  2:156536/1‑248 (MQ=255)                                                                                                                                                                                ggTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATCACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTc                                                            >  2:386250/1‑251 (MQ=255)                                                                                                                                                                                         cATATTAATACTAGCTCGTTCAATGTTGAGCTGGGCGCCAGCGACAAAAAAAGCGTCAAGCACGGTTGGTT‑GTAATCTTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGCATGGTAGCTATGGGTTATACAAATAATCTTTGCTCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGGCCGGAAt                                                   >  2:142367/1‑251 (MQ=255)                                                                                                                                                                                             gtaatattaGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTT‑GTAATATTCTTTCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTGCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGt                                               <  1:428454/250‑1 (MQ=255)                                                                                                                                                                                                          gTTCAATGGTGACTTGGGCGACAGAGACGACACAAGCGTCCAGCACGGTTGGTT‑GTAATCTTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCAGGGTCTGGTGAGCAAATAATATGATCGGCCAGATTCGCTATCGGTGATTGGTAGCTATGGGTTCTACAAATAATTTTTGCCCCGTTCGTTTTTGCCAGTTCTACTGCCGCTTTTACATCACTGGTTTGCCCGGTATGGGTCACTACCAgtcgc                                  >  2:333321/1‑247 (MQ=255)                                                                                                                                                                                                                         gggCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCtccttcct                     <  1:176987/249‑1 (MQ=255)                                                                                                                                                                                                                                          aaaGCGTACAGCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAcat  >  1:384978/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              GGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACAT  >  NC_000913/4311968‑4312451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑