Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,486,205	C→A	T29K (ACG→AAG)	crp →	DNA‑binding transcriptional dual regulator CRP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,486,205	0	C	A	97.8%	145.8 / NA	45	T29K (ACG→AAG)	crp	DNA‑binding transcriptional dual regulator CRP
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (24/20);  total (25/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

ACAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAA  >  NC_000913/3485959‑3486447                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   aCAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTGCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCt                                                                                                                                                                                                                                                 <  2:90956/250‑1 (MQ=255)   aGTAATACATTGATGTACTGCATGTACGCAAAGGACGTCACATTCCCGAGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGAGCATGGTGCTTGGCAAACCGCACACAGACACGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTAt                                                                                                                                                                                                                                              >  2:399772/1‑251 (MQ=255)   aGTAATACATTGATGTACTGCATGAATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGATATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTAt                                                                                                                                                                                                                                              >  2:162927/1‑251 (MQ=255)   aGTAATACATTGATGTACGGCATGTATGAAAAGGACGTCACATGACCGTGCAGTACATTGGATAGCCCCTTCCCAGTGAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGGTATCTGGCTCGGGAGAAAGCTTAGATCAGAGGATAGCCGCGCGTTGTTTTTGGCAAACCGCAAAAAGACCCGACTCTCGAATGTGTCTTGTCTCATTGCCACATTCATAAGTACGCATCCAAGATCAAGCTTAt                                                                                                                                                                                                                                              <  2:411267/251‑1 (MQ=255)              gATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGt                                                                                                                                                                                                                                    <  1:259168/250‑1 (MQ=255)                     tGCATGTATGAAAAGGACGTAACATTCCCGTGCAGTACAGTTAATAGCACATTCCCAGGTAGCGGGAAGCATATTTAGCCAATCCAGAGAAAGAGGCGATATCTGGCTCTGGAGAAAGCTTCTAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACACGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCAAACCAAGAGCAAGATTATTCCCCAGGGTGAAAAAGc                                                                                                                                                                                                                            >  2:442708/1‑251 (MQ=255)                       cATGTATGCAAAGGACGTCACATTTCCGTTCAGTACAGTGGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGGTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATATCCGCGCATGGTGCTTGTCATACCTCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCTCATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCgg                                                                                                                                                                                                                          <  1:444605/251‑1 (MQ=255)                          gTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGaa                                                                                                                                                                                                                        <  1:7745/250‑1 (MQ=255)                              gCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCt                                                                                                                                                                                                                   >  1:255327/1‑251 (MQ=255)                                       gTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGATGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACAt                                                                                                                                                                                                          <  2:151513/251‑1 (MQ=255)                                            aTTACCGGGCAGTACAGTTGATAGCCCCTTCCCAGGTAGGGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTAATAACAGAGGATAACCGCGCATGGTGCTTGGCAACCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTa                                                                                                                                                                                                     <  2:420353/251‑1 (MQ=255)                                                     cAGTACACTTGATAGCCCCTTCCCAGGTAGCGGGAAGCCTATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGACAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAA‑GGGGAAAAGCTGTACTAAATCGTTAAAGGATCtgg                                                                                                                                                                                           >  1:313584/1‑250 (MQ=255)                                                                atagcaccttcaaagctAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGATTATACCAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGACACGCTGTCCTACATCGTTAAAGGATCTGTGGCAGTGCTg                                                                                                                                                                                 >  2:276493/17‑251 (MQ=255)                                                                      ccTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCaaa                                                                                                                                                                           <  1:98950/251‑1 (MQ=255)                                                                         caccAGGTAGCGGGAAGCATATTTCGGCAAGCCAGAGACAGCGGCTTTATCTGGCTCTGGAGAAAGCTTATAACAAAGGATAACCGCGCAGGGTTCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAg                                                                                                                                                                          <  2:7569/247‑1 (MQ=255)                                                                          cccAGGTAGCGGGACGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAACGGCTCTGTGGCAGTGCTGATCAAAGACg                                                                                                                                                                       >  2:211274/1‑251 (MQ=255)                                                                             aGGTAGCGGGAAGCATATTTCGGCAATACAGAGACAGCGGCGTTATCTGGCTCTGGCGAAAGCTTATCACAGAGGATAACCGCGCCTGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGa                                                                                                                                                                      >  2:455184/1‑249 (MQ=255)                                                                                tggCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTAAAACAGAGGATAACCGCGCATGGTGCTGGGCAACCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTg                                                                                                                                                                                 <  2:151748/233‑1 (MQ=255)                                                                                tAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTg                                                                                                                                                                                 >  1:151748/1‑235 (MQ=255)                                                                                    gggAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGAAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTaa                                                                                                                                                             <  1:66834/251‑1 (MQ=255)                                                                                    gggAAGCATATTTCGGCAATCCAGAGACAGCGGCGGTATCTGGCTCTGGAGAAAGCTTATACCAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTaa                                                                                                                                                             <  1:186332/251‑1 (MQ=255)                                                                                                                            tGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTAtt                                                                                                                     >  1:109074/1‑251 (MQ=255)                                                                                                                                    gagaAAGCTAATAACAGAGGTGAACCGCGCATGTTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACt                                                                                                             <  2:286520/251‑1 (MQ=255)                                                                                                                                             gaaaaCAGAGAATAACCGCGCATGGTGCTGGGCAACCCGCAAGCAGACCCGACTCTCGAATGGTTCGTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGTGGAAAAAGCGGAAACGCTGTACTACATCGTGAAAGGTTCTGTGGCAGTGCGGATCAAAGACGAAGATTGTAAAGAAATGAGCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGt                                                                                                     <  1:453498/247‑1 (MQ=255)                                                                                                                                                    gagGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAag                                                                                               >  1:428386/1‑249 (MQ=255)                                                                                                                                                     agGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAgg                                                                                            >  1:54098/1‑251 (MQ=255)                                                                                                                                                      ggATAACCGCGCATGGAGATTGGCAAACCGCAAACAGAGCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTCTTCACCAGGGTGAAAATGCGGAAACGCTGTACTACATCGGTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGCAAAGAAACGATCCTCTCCTCTCTGAATAAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAAAggg                                                                                           >  2:326546/1‑251 (MQ=255)                                                                                                                                                          aaCCGCGCCTGGTGCTCGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTTAAGAGGGcc                                                                                         >  2:407456/1‑249 (MQ=255)                                                                                                                                                               cgcATGGTGCTTGTCAACCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCAACATTCATACGTACCCATCCCAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTATAGGCTCTGTGGCAGTGCTGATCAAAGACGCAGAGGGTAAAGAAATGATCCTCTCCTATCTGCATCAGGGTGATTTTATTGGc                                                                                                                  >  2:373285/1‑219 (MQ=255)                                                                                                                                                               cgcATGGTGCGTGGCAAACCGAAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGc                                                                                                                  <  1:373285/219‑1 (MQ=255)                                                                                                                                                                  aTGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCAcc                                                                                                                                                                                                                                         <  2:55786/97‑1 (MQ=255)                                                                                                                                                                  aTGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCAcc                                                                                                                                                                                                                                         >  1:55786/1‑97 (MQ=255)                                                                                                                                                                       gctaggaaaaCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCGATCTGAATCAGGGTGAGTTTATTGGCGAACTGGGCCTGTTTGAAGAAGGCCAGGAACGTATCGCAt                                                                          <  2:413261/244‑1 (MQ=255)                                                                                                                                                                              aaaCCGCAAACAGACCCGACTCTCGAATGGTTATTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGAGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACg                                                                   >  2:271603/1‑251 (MQ=255)                                                                                                                                                                               aaCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTTCTATCTGAATCAGGGTGATTTTATTGGCGAACTGTGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGt                                                                  <  1:211274/251‑1 (MQ=255)                                                                                                                                                                                aCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGGCACATTCATAAGTACCCATCCAAGAGCAAGCTTCTTCACCAGGGTGAAACAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCACGGAACGTAGCGCATGGGTACGTg                                                                 >  2:302355/1‑251 (MQ=255)                                                                                                                                                                                     caaCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGGGCGaa                                                             >  2:257307/2‑250 (MQ=255)                                                                                                                                                                                     aaaCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAACCGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGCTCAAAGACGAAGAGGGCAAAGAAATGATCCTCTCCTATCTGAATCAGGGGGATTTTATTGGCGAAATGGGCCTGTTTGAACAGGGCGCGGAACGTAGCCAATGGGtccgggcgca                                                             >  2:353395/1‑241 (MQ=255)                                                                                                                                                                                          gACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGGACGTGCGAAAACCGc                                                       >  1:332518/1‑251 (MQ=255)                                                                                                                                                                                           ccccGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACAGCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGcc                                                      <  1:407456/250‑1 (MQ=255)                                                                                                                                                                                               gACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGCACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCACTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCtgtg                                                  >  1:239246/1‑251 (MQ=255)                                                                                                                                                                                                                            acaTTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGACAAAGCGGAAACGCTGTCCTACATCGTTAAAGGCTCTGTGGCAGTGCTGCTCAAAGACGAAGCGGGTAAAGAACTGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAAttt                     >  1:53786/1‑251 (MQ=255)                                                                                                                                                                                                                                tCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAACAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGcc                 >  1:62919/1‑251 (MQ=255)                                                                                                                                                                                                                                               cAAGAGAAAGCTTATTCCCAAGGGTAAAAAAGGGAAAACGCGGTACTACATCGTAAAAGGCGCTGGGGCAGTGCTAATCAAAGACAAAGAGGGAAAAGAAAGTATCCTCTCCTATCGGAACAAGGTTAATTTTATTGGCGAACTGGGCTTGTTTAAAGAGGGCCAGAAACGTAGCGCATGGGTACGTGCGAAATCCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTaa  <  1:330274/251‑1 (MQ=255)                                                                                                                                                                                                                                                aaGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAAt              >  2:253243/1‑238 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   ACAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAA  >  NC_000913/3485959‑3486447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑