Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,196,861	A→G	intergenic (+79/+6)	C0293 → / ← ymfD	small regulatory RNA C0293/putative SAM‑dependent methyltransferase YmfD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,861	0	A	G	100.0%	33.3 / NA	14	intergenic (+79/+6)	C0293/ymfD	small regulatory RNA C0293/putative SAM‑dependent methyltransferase YmfD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/9);  total (5/9)

TATTAGGACAATTATGAATTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAGGCCATGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCC  >  NC_000913/1196639‑1197103                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   tattaGGACAATTATGAATTACCACTCCTTCCACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACGACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgct                                                                                                                                                                                                                         <  2:90442‑M1/250‑23 (MQ=255)  attaGGACAATTATGAATTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttc                                                                                                                                                                                                                       <  1:382108‑M1/251‑25 (MQ=255)                      ccACCCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGGATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACAGGGGGAATGTAAAAAAGGCCGCGAGCGCGGCCCTTTCACATACATGTTTAGTATTGAGACTGTTTAACCTAGGGAATTATGGTCGTActatcaacgattaaactgcgcttcggcgcagttttcgtttacag                                                                                                                                                                                                   <  1:433289‑M1/251‑45 (MQ=255)                                 acCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaa                                                                                                                                                                                          >  1:309544‑M1/1‑196 (MQ=255)                                       tCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaa                                                                                                                                                                                   >  1:27608‑M1/1‑190 (MQ=255)                                                             ttttGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgc                                                                                                                                                            >  1:242142‑M1/1‑168 (MQ=255)                                                                                                gACAGCCAACTGGCATTGGGTCCAATCTCCTTAGCGGTCAGTAACATGCGGAAGGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCGAGGGAATTATTGGCGTAccatcaacgattaaactgcgcttcggcccagtttccgtttacaggatggtgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgtgaaacctcatcctatgggagaac                                                                                                                         <  2:132503‑M1/251‑119 (MQ=255)                                                                                                              aTTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcag                                                                                                                                                                                                                >  2:348185‑M1/1‑119 (MQ=255)                                                                                                              aTTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcctcggcgcag                                                                                                                                                                                                                <  1:348185‑M1/150‑32 (MQ=255)                                                                                                                                   gTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcggggaaactccacctctgggggaaaaatgaataatggaatttcgggggtatcatctcccc                                                                                      >  1:174620‑M1/1‑98 (MQ=255)                                                                                                                                                                                             ttAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatgaataatgaaattgcggggttatcatctcccagtatatccatactaacaataaggttatttactcaaccaggcataaacattttgt                                <  1:242011‑M1/247‑208 (MQ=255)                                                                                                                                                                                                               aaCCTAGGGAATTATTGTCGTAccatcaacgattaaacggcgcttcggcgcagttgtcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggctggaaaccgcatcctatgggagaacaatgaataatgaaattgcggggttatcatctcccagtatatccatactaacaataaggtgagttactcaaccaggcagaaacattttgttttgtgcgtgggaacagcctt           <  2:174620‑M1/250‑229 (MQ=255)                                                                                                                                                                                                                      ggAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatgaataatgaaattgcggggttatcatctcccagtatatccatactaacaataaggttatttactcaaccaggcataaacattttgttttgtgcgtgggaacagccttaaggtgta   <  1:401801‑M1/251‑237 (MQ=255)                                                                                                                                                                                                                       gAATTATTGTCGTAccaccaacgattaaactgcgcttcggcgcagtattcggttacatgatgttgaaagggaaaattctggggcaaaaaaaccccgccagttacggcgggaaacctcagcctacgggagaacaatgaataatgaaattgcggggttatcatctcccagcatatccatactaccaataaggttattttctcaaccatgcataaacatgttgttttgtgcgtggtaacagtcttaaggtctaa  <  2:360386‑M1/251‑238 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   TATTAGGACAATTATGAATTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAGGCCATGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCC  >  NC_000913/1196639‑1197103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑