Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	670,635	A→T	L324Q (CTG→CAG)	holA ←	DNA polymerase III subunit delta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	670,635	0	A	T	100.0%	214.3 / NA	57	L324Q (CTG→CAG)	holA	DNA polymerase III subunit delta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (30/27);  total (30/27)

CATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCAGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGTTGTAATGTGCGCAACA  >  NC_000913/670396‑670869                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            cATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCcac                                                                                                                                                                                                                                  >  1:415131/1‑251 (MQ=255) cATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCcac                                                                                                                                                                                                                                  >  1:5978/1‑251 (MQ=255)         tACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACactgact                                                                                                                                                                                                                           <  1:46285/250‑1 (MQ=255)                               cGGCTGGGGACGATGCGGCGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTg                                                                                                                                                                                                    >  2:331377/1‑251 (MQ=255)                                        aCGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGtt                                                                                                                                                                                           <  1:80093/251‑1 (MQ=255)                                            tGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCgtg                                                                                                                                                                                       >  1:364979/1‑251 (MQ=255)                                             gCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCgtgt                                                                                                                                                                                      >  1:39198/1‑251 (MQ=255)                                                  ggaACATTATTAGGGATGATGGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGa                                                                                                                                                                                 <  2:372173/251‑1 (MQ=255)                                                              gggATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCt                                                                                                                                                                     >  2:132922/1‑251 (MQ=255)                                                              gggATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATGTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCGCACTGACTGACTGTAATCTGGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCt                                                                                                                                                                     <  2:426881/251‑1 (MQ=255)                                                                 atgatTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATc                                                                                                                                                                                                                 <  2:69694/204‑1 (MQ=255)                                                                 atgatTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATc                                                                                                                                                                                                                 >  1:69694/1‑204 (MQ=255)                                                                  tgatTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTACTCTTGTTTGAGGGTGAGTTCCGTTCGTGTCTGGAGTTGCACGGCCTGACg                                                                                                                                                                 >  2:253034/1‑251 (MQ=255)                                                                                gTCAGACCAATTACATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGAGCACCGGAGCCAAGGTGCCGCCAAAACGAGCCTGTAAAGATTTCATATCAACCGTCGATCGATACGTCCGCCAGGGGTTTATGGCACAACAGAAGCGATAACCCTTCCTGCTCTGCCAACACTGACTGACCGGAATCTTGTTTGCTGGTGAGTTCCGTTCGCGTCAGGAGTTGCACGGCTTGACTTCACTGTGTCttac                                                                                                                                                   >  2:131818/1‑248 (MQ=255)                                                                                      ccAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGc                                                                                                                                             <  1:337697/251‑1 (MQ=255)                                                                                         agcAAATTCGCCAGCGTTTCCACGGGTTGTAGATGACCATAGTGCACCGGATCAAAGGGGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGATATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAAGTGCGTCTGACTTAAGCGAt                                                                                                                                          <  2:468847/249‑1 (MQ=255)                                                                                                 cGCCAGCGTTTCCCCGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGcc                                                                                                                                  <  2:374495/251‑1 (MQ=255)                                                                                                  cccAGCGTTTCCGCGCGTTTTAGATGTGCATAGGGCACCGGGTCAAAGAGGCCGCCAAACAGAGCCGGTAAAGATTTCATATCAACAGTCGATAAATACGTCCGCCAGGGGTTTGTGGCCCAACAGAAGAGATATCCCTTCCTGCTCTGCCCACACTGACTGACCGTATTCTGGGTTGAGGGTGAGTTCCGTTCGGGGCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAGGCGGTTTAACgtct                                                                                                                                 <  2:252555/250‑4 (MQ=255)                                                                                                      gCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTTTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGcc                                                                                                                             <  2:420927/251‑1 (MQ=255)                                                                                                            ccACGGGCTTTAGATGTCCATAGAGCACCGGATCAAAGGCGCCGCCAAACAGCGCGTGTCAAGATTTCATATCAACCGTCGATCAATCCGTCCGCCAGGGGTTTCTGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGCCGTAACTGCGTCTGACTTACGCGCTTTAACGCCTCGCCcatcat                                                                                                                       >  2:55050/1‑251 (MQ=255)                                                                                                                gggTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGccc                                                                                                                   >  2:278077/1‑251 (MQ=255)                                                                                                                     ttAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCcggcg                                                                                                              >  1:405281/1‑251 (MQ=255)                                                                                                                            aCCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGc                                                                                                      <  1:132922/251‑1 (MQ=255)                                                                                                                               aTAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCAt                                                                                                   >  2:91132/1‑251 (MQ=255)                                                                                                                                    gCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATAccc                                                                                               <  1:199213/250‑1 (MQ=255)                                                                                                                                      aCCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCGGCCCACACTGACTGACCGGAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGCATTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGGTC‑TGGCATACCCg                                                                                              >  2:104090/1‑249 (MQ=255)                                                                                                                                         ggATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCCTATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCATTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGCGAGTTCCGTTCGTGTCAGGCGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATACCCGATGCt                                                                                         >  1:410115/1‑251 (MQ=255)                                                                                                                                         ggATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCGATACCCGATGCt                                                                                         <  2:335288/251‑1 (MQ=255)                                                                                                                                          gATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATACCCGATGCtt                                                                                        <  1:423452/251‑1 (MQ=255)                                                                                                                                                 ggTGCCTCCAAACAGAGCCTGTACAGATTTCATATCAACCGTCGATCAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTCACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCCTCATGCCCCGGCGGTTCGTGCC‑TACCCGATGCTTATGaaa                                                                                  >  2:274395/1‑250 (MQ=255)                                                                                                                                                    gccgccAAACAGTGCCTGTAAAGATTTCATATCAACCGTCGATAAATACTGCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAAGCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCGGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATTCCCGATGCTTATCAaacaac                                                                              <  2:57768/251‑1 (MQ=255)                                                                                                                                                             cAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATACCCGATGCTTATCAAACAACGCACGCAGt                                                                     <  1:278077/251‑1 (MQ=255)                                                                                                                                                              agagCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATACCCGATGCTTATCAAACAACGCACGCAGTg                                                                    <  1:253034/251‑1 (MQ=255)                                                                                                                                                                     atAAAGATTTCATAGCAACCGGCGATAAATACGTCCGCCAGGGGTTTATGGCCCAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTa                                                               <  2:75940/248‑1 (MQ=255)                                                                                                                                                                       aaaGATTTCATATCAACTGTCGATAAATACGTCCGCCAGGGGATTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGCCTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGCCGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGACCCGGCGGTTC‑TGCCATACCCCCTTCTTATCAAACAACGCACGCAGTGGCGTTTggg                                                           >  2:72761/1‑251 (MQ=255)                                                                                                                                                                              tCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATACCCGGTGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACt                                                     <  1:51015/250‑1 (MQ=255)                                                                                                                                                                              tCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACt                                                     <  1:251681/250‑1 (MQ=255)                                                                                                                                                                                        cGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTccgg                                          >  2:462750/1‑248 (MQ=255)                                                                                                                                                                                        cGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAgg                                          >  1:360858/1‑251 (MQ=255)                                                                                                                                                                                         gTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGCTAACCCTTCCTGCTCTGCCCACACTGACTGACCGTACTCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCCTCATGCCCCGGCGGTTC‑TGCCATACCCGCTGCTTATCAAACCACGCACGCAGTGGCGTCTGGGCCGACTGGCGTTTCCGGt                                         >  2:273722/1‑251 (MQ=255)                                                                                                                                                                                               aaaTACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGTAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACTCCTCGCTCATCATGCCCCGGCGGTTC‑TTCCATCCCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCa                                   <  1:292359/251‑1 (MQ=255)                                                                                                                                                                                                aaTACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCCGACTGGCGTTTCAGGTTAAcc                                    >  1:309926/1‑249 (MQ=255)                                                                                                                                                                                                     gTCCGCCAGGGGGTTGTGGCAAAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGACTTCCGTTCGTGCCAGGAGTTGCACGGCCTGACGTACATGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGATC‑TGCCATAACCGATGCTTATCAACCAACGCACACCGAGGCGTATGGGCCGCCTGGGTTTTCCGGTTaagcaagaac                              >  2:195464/1‑242 (MQ=255)                                                                                                                                                                                                     gTCCGCCAGGGGCTTCTGGCACAACAGATGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTCATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCTGCGGTTC‑TGCCCTACCCGATTCTTATCAACCACCGCACGCAGTTGCGTATGGGCAGACTGGCGTTTCAGGTTGACCAGttac                              >  2:470054/1‑247 (MQ=255)                                                                                                                                                                                                       ccGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTaacaac                           <  1:91132/251‑1 (MQ=255)                                                                                                                                                                                                         gCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAg                         <  1:331377/251‑1 (MQ=255)                                                                                                                                                                                                              gtgTTTATGGCACAACAGAAGAGATAACCCTTCCTTCTCTGCCCACACTGACTTACCGTAATCTTGTTTTAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTATCGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCTTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCAc                    <  1:227041/249‑1 (MQ=255)                                                                                                                                                                                                                gTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATACCCGATGCTTATCAAACAACGAACGCAGGGGCGTATGGGCAGACTGGCGTTTACGGTTAACCAGTAACAACAGGTCACGt                  >  2:82144/1‑251 (MQ=255)                                                                                                                                                                                                                    aTGGCACAACAGCAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATACCCGCTGCTTATCAAACAACGCCCGCAGTGGCGTATGGCTCGACCGGCGTTTCCGGATAACCAGTAACACCAGTTCACGTTga               >  2:348061/1‑249 (MQ=255)                                                                                                                                                                                                                    aTGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGTGCTGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGTTGt               <  1:73373/250‑1 (MQ=255)                                                                                                                                                                                                                           aacaGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATACCCGATGCTTATCAAACAACGCACGCAGGGGCGTCTGGGCAGACTGGCGTTTCAGGCTAACCAGTAACAACAGTTCACGCTGTAATGTgcg       >  2:9934/1‑251 (MQ=255)                                                                                                                                                                                                                            acaGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGTTGTAATGTgcgc      <  2:39198/251‑1 (MQ=255)                                                                                                                                                                                                                              agaagaGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGTTGTAATGTGCGca     >  1:321047/1‑250 (MQ=255)                                                                                                                                                                                                                                aagaGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCCCGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATACCCGATGCTTATCAAACAACGCACGCAGGGGCGTATGGGCAGCCTGGCGTTTCAGGTTCACCAGCACCAACAGTTCCCGTTTTAATGTGCGcaac   >  1:417860/1‑250 (MQ=255)                                                                                                                                                                                                                                 agagATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATACCCGATGCTTATCAAACAACGCACGCCGGGGCGTATGGGCCGACTGGCGTTTCAGGTTAACCAATAACAACAGTTCACGTTGTAAttgtcggcacc  >  2:11865/1‑240 (MQ=255)                                                                                                                                                                                                                                        aCCCTTCCTGCTCTGCCCACCCTGACTGACCGTAATCTTGTTTGAGGGCGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGt                  >  2:234083/1‑227 (MQ=255)                                                                                                                                                                                                                                        aCCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGt                  <  1:234083/227‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            CATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCAGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTC‑TGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGTTGTAATGTGCGCAACA  >  NC_000913/670396‑670869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑