Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,174,626	0	G	A	30.0%	87.4 / 13.7	50	F180F (TTC→TTT)	ycfT	inner membrane protein YcfT
Reads supporting (aligned to +/- strand):  ref base G (30/5);  new base A (0/15);  total (30/20)
Fisher's exact test for biased strand distribution p-value = 6.89e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACC‑AGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCA‑GCAGATGGCGGCGCAACGG‑CACCTCTTTAA‑CACAGGTCATTATTGTTGCGCCAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAA‑TCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCA  >  NC_000913/1174381‑1174867                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    aaTCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACC‑AGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCA‑GCAGATGGCGGCGCAACGG‑CACCTCTTTAA‑CACAGGTCATTATTGTTGCGCCAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAAtt                                                                                                                                                                                                                                                >  1:425575/1‑251 (MQ=255)     gTTGCTCGTATTGATAAAACAGCTTCATGATCACCACACTCGATACCAGCGACCACAGCAGCGAGATAGTCAACAACC‑AGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCA‑GCAGATGGCGGCGCAACGG‑CACCTCTTTAA‑CACAGGTCATTATTGTTGCGCCAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAATT‑AAcc                                                                                                                                                                                                                                           >  1:429337/1‑251 (MQ=255)            gTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACC‑AGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCA‑GCAGATGGCGGCGCAACGG‑CACCTCTTTAA‑CACAGGTCATTATTGTTGCGCCAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAATT‑AACCGCCacac                                                                                                                                                                                                                                    >  1:201324/1‑251 (MQ=255)                           cTTCATGATCACCACAATCGATACCAGCTACAACAGCAGCGAGATAGTAAACAACC‑AGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCA‑GCAGATGGCGGCGCAACGG‑CACCTCTTTAA‑CACAGGTCATTATTGTTGCGCCAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAATT‑AACCGCCACACTAAGCAGTccaaac                                                                                                                                                                                                                      >  2:257343/1‑250 (MQ=255)                           cTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACC‑AGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCA‑GCAGATGGCGGCGCAACGG‑CACCTCTTTAA‑CACAGGTCATTATTGTTGCGCCAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAATT‑AACCGCCACACTCAGCAGTacaaac                                                                                                                                                                                                                      >  1:260170/1‑250 (MQ=255)                              cATGATCCCCAAAATCGAAACCAGCGAAAAAAGCAGCGAGATAGAAAACACCC‑AGGCACCGACCGCCAGAACGGTAAGAAAAGAAGCAATCA‑GCAGAGGGCGGCGCAACGG‑CCCCTTTTTAA‑CACAGGTCATTATTGTTGCGCAAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGAAAATGGCGGATCACACTGTT‑CATCCCCCCCCGCGGCGGGGGAAC‑GAAATT‑AACCGCCACACTCAGCAGTACAAACAAg                                                                                                                                                                                                                   <  1:314172/250‑1 (MQ=255)                                    caccacAATAGATACCAGCGACAACAGCAGCGAGATAGTAAACAACC‑AGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCA‑GCAGATGGCGGCGCAACGG‑CACCTCTTTAA‑CACAGGTCATTATTGTTGCGCCAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CCTTCACCACCACGGCGTGGGCAC‑GAAAGT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAAt                                                                                                                                                                                                             >  2:317355/1‑250 (MQ=255)                                     accacAATCGCTACCAGCGACAACAGCAGCGAGATAGTAAACAACC‑AGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCA‑GCAGATGGCGGCGCAACGG‑CACCTCTTTAA‑CACAGGTCATTATTGTTGCGCCAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAAt                                                                                                                                                                                                             >  2:47555/1‑249 (MQ=255)                                               aTACCAGCGACAACAGCAGCGAGATAGTAAACAACC‑AGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCA‑GCAGATGGCGGCGCAACGG‑CACCTCTTTAA‑CACAGGTCATTATTGTTGCGCCAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGcc                                                                                                                                                                                                 >  1:169483/1‑251 (MQ=255)                                                 aCCAGCGACAACAGCAGCGAGAAAGTAACCAACC‑AGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCA‑GCAGATGGCGGCGCAACGG‑CACCTCTTTAA‑CACAGGTCATTATTGTTGCGCCAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGcc                                                                                                                                                                                                 >  1:396552/1‑249 (MQ=255)                                                       gaaaacaGCAGCGAGAAATAAAACACCC‑AGCCACCGACCGCCAGAACGGAAAGAAAAGAAGCCACCA‑GCAGAGGGCGGCG‑AACGGGCCCCTTTTAAA‑AACAGGTCATTATTGTTGCGCCAAACC‑ACGCGC‑AAGG‑GTGTTAATAGGGCAAAGTCCGGATCACCCTGTT‑CATCCCCCCCCCGGGCGGGGAAAC‑AAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCt                                                                                                                                                                                         <  1:210661/248‑1 (MQ=255)                                                        aaaacaGCAGCGAGATAGTAAACACCC‑AGGCCCCGCCCGCCAAAACGGTAAGCAAAGAAGCCATAA‑GCAGATGGCGGCGCAACGG‑CCCCTCTTTAA‑CACGGTTCATTTTTGTTGCGCAAAACC‑ATGCGCAAAGG‑CTTA‑AAAAAGGCAAATGGCGGATCACACTGTT‑CATCCCCCCCCGCGGCGTGGAAAC‑AAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTa                                                                                                                                                                                        <  1:161636/249‑1 (MQ=255)                                                         caacaGAAGCGAAATAAAAAACACCA‑AGGCACCGCCCGCCAGAACGGAAAGAAAAGAACCCATCA‑GCAGATGGCGGCGCAACGG‑CACCTTTTTAA‑CACAGGTCTTTATTGTTGCCCCAAACC‑ATGCGCAAAGG‑CTGA‑AAAAAGGAAAATTGCGGATCACACTGTT‑CATTCCCCCCCACGGCGGGGAAAC‑GAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTaa                                                                                                                                                                                       <  1:47555/251‑1 (MQ=255)                                                         aaaacagaagggaaatagaaaCAACCCAGGCCCCGACCGCAAAAACGGAAAGAAAAGAA‑CCAACAAGCAGTTGGCGGCGAAACGG‑CACCTCTTAAA‑CAAGGGTCATTTTTGTGGCCCAAAACC‑AGGCGCAAAGG‑CTGA‑AATAAGGAAAATTGGGGACCACCCTGTT‑CATCCCCCCCCCCGGCGGGGGAAC‑AAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTa                                                                                                                                                                                        <  1:253940/233‑1 (MQ=255)                                                          aacaGCAGCGAGATAGTAAACAACC‑AGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCA‑GCAGATGGCGGCGCAACGG‑CACCTCTTTAA‑CACAGGTCATTATTGTTGCGCCAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTaa                                                                                                                                                                                       >  1:101316/1‑250 (MQ=255)                                                                 gcGAGATAGTAAACAACC‑AGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCA‑GCAGATGGCGGCGCAACGG‑CACCTCTTTAA‑CACAGGTCATTATTGTTGCGCCAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAAtttt                                                                                                                                                                                >  1:99600/1‑250 (MQ=255)                                                                   gagaTAGTAAACAACC‑AGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCA‑GCAGATGGCGGCGCAACGG‑CACCTCTTTAA‑CACAGGTCATTATTGTTGCGCCAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTAc                                                                                                                                                                              >  1:403043/1‑250 (MQ=255)                                                                       tAGTAAACAACC‑AGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCA‑GCAGATGGCGGCGCAACGG‑CACCTCTTTAA‑CACAGGTCATTATTGTTGCGCCAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATAcc                                                                                                                                                                         >  2:12204/1‑251 (MQ=255)                                                                           aaaccaacaggcccccgcccgCCAGAACGGTAACAAAAAAAGCCAACA‑GCATTTGGCGGCG‑AACCG‑CCCCTTTTTAAACACAGTACTTTTTTGTTGCCCCAACCC‑ATCCCCCAAGG‑TTGA‑AAAAAGGAAATTTGCGGATCCCTCTGTT‑CATCCCCCCCCCGGGGGGGGGAAC‑AAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACg                                                                                                                                                                      <  1:36954/233‑1 (MQ=255)                                                                               aacC‑AGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCA‑GCAGATGGCGGCGCAACGG‑CACCTCTTTAA‑CACAGGTCATTATTGTTGCGCCAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCCACCGGCTAAAAATTTTACATAcaaacaaat                                                                                                                                                                  >  2:7654/1‑242 (MQ=255)                                                                                      gccccgcccgCCAGAACGGAAAGCAAAGAAGCCATCA‑GCAGATGGCGGCGCAACGC‑CCCCTCTTTAA‑CACAGGTCATTATTGTTGCGCCAAACC‑AGGCGCAAAGG‑CTGA‑AATAAGGAAAATTGCGGATCACCCTGTT‑CATCCCCCCCCACGGCGTGGGAAC‑AAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAAt                                                                                                                                                           <  1:52362/244‑1 (MQ=255)                                                                                         cgaaccgccgaaagggaaagaaaaGAACCCACCA‑GAAGATGGCGGCCCAACGC‑CCCCTTTTAAA‑CACAGTTCTTTGTTGTTGCGCCAAACC‑ATGC‑CCAAGGGCTAA‑AAAAAGGAAAATGGCGGATCACATTTTT‑CATTCCCCCCCACGGCGGGGGAAC‑GAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTaa                                                                                                                                                        <  1:68816/230‑1 (MQ=255)                                                                                         ccgaccgCCAGAACGGTAAGCAAAGAAGCCATCA‑GCAGATGGCGGCGCAACGG‑CACCTCTTTAA‑CACAGGTCATTATTGTTGCGCCAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTa                                                                                                                                                         >  1:37092/1‑250 (MQ=255)                                                                                          caacccccaaaaCGGTAAGCAAAGAAGCCATCA‑GCAGATGTCGGCGCAACGC‑CCCCTCTTTAC‑CACAGGTCATTTTTGTTGCCCCAAACC‑AGGCGCCAAGG‑CTTA‑AAAAAGGCAAATTGCGGATCCCATTGTT‑CATCCCCCCCCGCGGCGGGGAAAC‑AAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAAc                                                                                                                                                       <  1:301802/241‑1 (MQ=255)                                                                                              tgccgatcgggaaaGCAAAGAAGCCATCA‑GCAGAGGGCGGCGCACCGG‑CCCCTTTTTAA‑CAGAGTTCATTTTTTTTGC‑CCAAACCCATGCGCCAGGG‑ATAA‑AAAAGGGCAAATTGCGGACCACCCTTTT‑CATCCCCCCCCACGGC‑TGGAAAAAGAAATA‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGc                                                                                                                                                     <  1:350843/237‑1 (MQ=255)                                                                                              cgCCAGAACGGTAAGCAAAGAAGCCATCA‑GCAGATGGCGGCGCAACGG‑CACCTCTTTAA‑CACAGGTCATTATTGTTGCGCCAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCAt                                                                                                                                                   >  1:23200/1‑251 (MQ=255)                                                                                                      gggaaaacAAAGAAGCCAACA‑GCAGATGGCGGCGCAACGG‑CCCCTCTTTAA‑CAAAGGTCATTATTTTTGC‑CCAAACCCATGCGCCAAGG‑GTGA‑AAAAAGGAAAATTGCGGATCCCCCTGTT‑AATCCCCCCCCCCGGGGTGGGAAC‑AAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATAc                                                                                                                                           <  1:257343/244‑1 (MQ=255)                                                                                                       ggTAAGCAAAGAAGCCATCA‑GCAGATGGCGGCGCAACGG‑CACCTCTTTAA‑CACAGGTCATTATTGTTGCGCCAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGc                                                                                                                                                     >  2:350843/1‑240 (MQ=255)                                                                                                       ggTAAGAAAAAAAGCCATCA‑GCAGATGGCGGCGCAACGG‑CCCCTCTTTAA‑CACAGGTCATTTTTGTTGCCCCAACCC‑AGGCCCCAAGG‑CTGT‑AAAAAGGCAAATTGCGGACCACCCTGTT‑CATCCCCCACCCCGGGGTGGAAAC‑AAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATAcc                                                                                                                                          <  1:141694/251‑1 (MQ=255)                                                                                                                     ccATCA‑GCAGATGGCGGCGCAACGG‑CCCCTCTTAAA‑CACAGGTCATTATTGTTGCGAAAAACC‑AGGCGCCAAGG‑CTGA‑AATAAGGAAATTTGGGGACCCCACTTTT‑CATCCCCCACCACGGCGTGGAAAC‑AAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTg                                                                                                                            <  1:349845/251‑1 (MQ=255)                                                                                                                            gcagGTGGCGGCGCACCCA‑CTCCTCTTAAA‑CACAGGACATTATTTTTCCGCAAACCC‑AGGCGCCAAGG‑CGGA‑AATAAGCAAAATGGCGAACCCCCCTTTT‑CTCCCCCCCCCGCGG‑GTGGGAACCAAAATA‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTg                                                                                                                      <  2:23200/251‑1 (MQ=255)                                                                                                                                        gcAACGG‑CACCTCTTTAA‑CACAGGTCATTATTGTTGCGCCACACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCACATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAAtt                                                                                                                                                                                                                                                >  2:236036/1‑118 (MQ=255)                                                                                                                                        gcAACGG‑CACCTCTTTAA‑CACAGGTCATTATTGTTGCGCCAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAAtt                                                                                                                                                                                                                                                <  1:236036/118‑1 (MQ=255)                                                                                                                                          aaCGG‑CACCTCTTTAA‑CACAGGTCATTATTGTTGCGCCAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGGCAATTAACGCATACAGATACCACCAGCTGGTGCTGGCggggctcaatccgtgc                                                                                                          >  2:325326/1‑235 (MQ=255)                                                                                                                                          aaCGG‑CACCTCTTTAA‑CACAGGTCATTATTGTTGCGCCAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGGGATCATCCCGTGc                                                                                                          >  1:118215/1‑249 (MQ=255)                                                                                                                                              g‑CACCTCTTTAA‑CACAGGTCATTCTTGTTGCGCCAAACC‑ATGCGCCAACG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACTACGGCGTGGGAAC‑GAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGAGAATAGTGGAAGCGCCAGACGGCTAAAAATTTTACAGACCACGAAATAGACAATTAACGCATACAGATACCACAAGCAGGTGCTGGCGGTGagcaaccccggccggaac                                                                                                    >  2:279223/1‑234 (MQ=255)                                                                                                                                              g‑CACCTCTTTAA‑CACAGGTCATTATTGTTGCGCCAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCCTGCCGGAAc                                                                                                    >  1:341297/1‑251 (MQ=255)                                                                                                                                                        taaacaaagtggtttatggtggcgcCACACC‑AAGCGACAAGG‑CTGT‑AATAAGGCAAAGTGCGGCTCACA‑TGTTTCACTCCCCCCCAGGGCGTGG‑AAG‑AAAATTTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCg                                                                                              <  2:101316/228‑1 (MQ=255)                                                                                                                                                          a‑cacAGGTCATTATTGTTGCGCCAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GCAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCCGGAACTCACCGGTggg                                                                                         >  1:35562/1‑250 (MQ=255)                                                                                                                                                                 gTCATTATTGTTGCGCCAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAA‑TCGGc                                                                                  >  1:106708/1‑251 (MQ=255)                                                                                                                                                                             gccccAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGAAAATGGCGGATCACATTTTT‑CATCCCCCCCCACGGCGTGGAAAC‑AAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAA‑TCGGCATAAGCGGCAt                                                                       <  1:461131/247‑1 (MQ=255)                                                                                                                                                                                  aaaCC‑ATGCGCCAAGG‑CTGC‑CCTAAGGCCAATTGCGGCTCACACTGTT‑CATTCCCAACCACGGCGTGGGAAC‑GCAATT‑AACCGCCACACTCAGAAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCGAAAAATTTTACCTACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGAGAGTCCCATGCCGGAACACACCGATGGCA‑ACGGCCTAAGCgggaattgagg                                                                 >  2:460832/1‑242 (MQ=255)                                                                                                                                                                                        aTGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAATT‑AACCGCCACACTCAGCCGTCCAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCCGGCAGGAAACCCCCGGTGGAA‑TTCGCATAAGCCGCATTGGAGGCAttt                                                            >  1:216448/1‑250 (MQ=255)                                                                                                                                                                                             ccAAGG‑CTAA‑AAAAAGGCAAATGGCGGATCAC‑CTGTTTCGCTCACCCCCACGGCGGGGAAAC‑AAAATA‑AGCCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAA‑TCGGCATAAGCGGCATTGGAGGCATTGCTTaa                                                       <  2:349553/251‑1 (MQ=255)                                                                                                                                                                                                  aatctgaaataaGACAAATTGCTGATCACACTGTT‑CCTCCCCCACCCCGGCGTGGTACC‑AAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAA‑TCGGCATAAGCGGCATTGGAGGCATTGCTTAAATc                                                    <  1:101797/244‑1 (MQ=255)                                                                                                                                                                                                         aataaGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAATT‑AACCGCCACACTCAGCAGTCCAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTACAAAt                                                                                                                                                                                   >  1:192750/1‑118 (MQ=255)                                                                                                                                                                                                              ggCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCCTCCCGTGCAGGAACTCACCAGTGGAAATCG‑CAAAAGCGGCATTGGAGGCCATGCTTaaaacccgccgccgagc                                        >  2:146906/1‑237 (MQ=255)                                                                                                                                                                                                                        ggATCACACTTTC‑CATCCCCCCCCCCGGCGTGGAAAC‑AAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAA‑TCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTgg                              <  1:12204/251‑1 (MQ=255)                                                                                                                                                                                                                                                     cGTGGGAAC‑GAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGGGGAA‑TCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGGGCCAGCCACTGGGTGAGCGCACTTAACGCCCGCCACTGcc  >  1:447943/1‑250 (MQ=255)                                                                                                                                                                                                                                                        gggAAC‑GAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCt                                                                                                                             >  2:331966/1‑126 (MQ=255)                                                                                                                                                                                                                                                               gAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCa                                                                                                                                                                        >  1:350757/1‑77 (MQ=255)                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    AATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACC‑AGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCA‑GCAGATGGCGGCGCAACGG‑CACCTCTTTAA‑CACAGGTCATTATTGTTGCGCCAAACC‑ATGCGCCAAGG‑CTGT‑AATAAGGCAAATTGCGGATCACACTGTT‑CATTCCCCACCACGGCGTGGGAAC‑GAAATT‑AACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAA‑TCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCA  >  NC_000913/1174381‑1174867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑