Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,707,484	+GA :: IS3 (+) +4 bp	coding (219‑222/1608 nt)	dppA ←	dipeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	315229 =	NA (NA)	30 (0.660) +TC	29/480	0.5	100%	noncoding (1/1255 nt)	IS3	repeat region
	?	NC_000913	= 3707487	0 (0.000)					coding (219/1608 nt)	dppA	dipeptide ABC transporter periplasmic binding protein
*	?	NC_000913	= 2171428	NA (NA)	32 (0.700)	31/484	0.4	100%	noncoding (1258/1258 nt)	IS3	repeat region
	?	NC_000913	3707484 =	0 (0.000)					coding (222/1608 nt)	dppA	dipeptide ABC transporter periplasmic binding protein

TGAAACTGAGCGCCAATGGTTATATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGTCTTCT  >  NC_000913/314979‑315470                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   ggAAACTGAGCGCCAAGGGTTATATACGGGCTTCGGGCTGGAAAAAAGCGCCGGAAATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTTCTGGGGGCTATTTTCCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCct                                                                                                                                                                                                                                                    <  1:317559/250‑1 (MQ=255)  gAAACTGAGCGCCAATGGTTATATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCctc                                                                                                                                                                                                                                                   >  1:218870/1‑251 (MQ=255)                 tGGTTATATTCGGGCTTCTGGCTGGAAAAAATAGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGATATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGt                                                                                                                                                                                                                                    >  2:466282/1‑251 (MQ=255)                            gggCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTCTCAGGACCGCCCGGCGAATGGCTGGGCTATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGc                                                                                                                                                                                                                         >  1:322436/1‑251 (MQ=255)                             catgccttcgaagtattcgtagctgccgccagaaactttatggtacgggttttgcgcgtttttctgacgatcgaacgagaacaccacatcatcggcgttcagttcacgcgtcggtttgaattctttattgtcgtgccacttcacacctttacgcagatggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCt                                                                                                                                                                                                                        >  2:123207‑M1/223‑251 (MQ=255)                               tgccttcgaagtattcgtagctgccgccagaaactttatggtacgggttttgcgcgtttttctgacgatcgaacgagaacaccacatcatcggcgttcagttcacgcgtcggtttgaattctttattgtcgtgccacttcacacctttacgcagatggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTa                                                                                                                                                                                                                       <  2:231740‑M1/30‑1 (MQ=255)                                   tggctggAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTg                                                                                                                                                                                                                   >  1:345897/1‑250 (MQ=255)                                        agtattcgtagctgccgccagaaactttatggtacgggttttgcccgtttttctgacgatcgaacgagaccaacacatcatcggcgttcagctcacgcgtcggtttgaattctttattgtcgtgccacttcacacctttacgcagatggcaggtataggttttaccgtcttcgctgagttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTaa                                                                                                                                                                                                              <  1:32179‑M1/39‑1 (MQ=255)                                         gtattcgtagctgccgccagaaactttatggtacgggttttgcgcgtttttctgacgatcgaacgagaacaccacatcatcggcgttcagttcacgcgtcggtttgaattctttattgtcgtgccacttcacacctttacgcagatggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAAc                                                                                                                                                                                                            <  2:73496‑M1/41‑1 (MQ=255)                                                 cGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCtcagt                                                                                                                                                                                                     <  1:245145/250‑1 (MQ=255)                                                     ggATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCtcagtcag                                                                                                                                                                                                  >  1:233482/1‑249 (MQ=255)                                                           tGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGAc                                                                                                                                                                                           <  1:291790/250‑1 (MQ=255)                                                              ggATTATCACGAACGCCCGGCGAATGGCTGGGATATTCGTCATGAGGGCTATTTACCCGCCTGGCCGCAGCTCGGCGCAAGCCTGATGTATGAACAGTATTCTGGCGCTGAAGTCGGGCTGTTTGGTAAAGATAAGCGCAAGAAAGACCCGCATGCTATTTATGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAACCTCTCAGTCAGAGGCTACTcac                                                                                                                                                                                       >  2:282956/1‑251 (MQ=255)                                                               gATTATCAGGACCGCCCGGCGAATGGCTGGGATATTCGTGCTGCGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGCAGTCGGGCTGTTTGGTAAAGATAAGCGCCCGCAGGCCCCGCATGCGATTTCTGCCGAGGAGACCTATACGCCCATGCCTCTTACCCCCAAATAGTGGACCAGCGGCTCAGTGCGTACACTCTCCGTCAGAggggcctcaac                                                                                                                                                                                      >  2:190205/1‑242 (MQ=255)                                                                         acgggttttgcgcgtttttctgacgatcgaacgagaacaccacatcatcggcgttcagttcacgcgtcggtttgaattctttattgtcgtgccacttcacacctttacgcagatggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAAc                                                                                                                                                                            >  1:334692‑M1/179‑251 (MQ=255)                                                                         acgggttttgcgcgtttttctgacgatcgaacgagaacaccaaatcatcggcgttcagttcacgcgtcggtttgaattctttattgtcgtgccacttcacacctttacgcagatggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAAc                                                                                                                                                                            <  1:315029‑M1/73‑1 (MQ=255)                                                                          aCGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACa                                                                                                                                                                           <  1:466282/251‑1 (MQ=255)                                                                            gCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGt                                                                                                                                                                         <  1:33345/251‑1 (MQ=255)                                                                                ggCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATc                                                                                                                                                                      >  1:185451/1‑250 (MQ=255)                                                                                  gcgcgtttttctgacgatcgaacgagaacaccacatcatcggcgttcagttcacgcgtcggtttgaattctttattgtcgtgccacttcacacctttacgcagatggaaggtataggttttaccgtctgcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAATACAGTATCAAc                                                                                                                                                                   >  2:351220‑M1/170‑251 (MQ=255)                                                                                   gAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGCGACCTATACGCCAGTGCCTCTTACCCAGCAATAATGGACACGCGGTTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATAAAc‑c                                                                                                                                                                 >  2:115280/1‑251 (MQ=255)                                                                                        gCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTa                                                                                                                                                             >  1:19549/1‑250 (MQ=255)                                                                                            ggATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAAAAGTATCAAC‑CAGTaaaaa                                                                                                                                                         >  2:131735/1‑250 (MQ=255)                                                                                                      aacgagaacaccacatcatcggcgttcagttcacgcgtcggtttgtattctttattgtcgtgccacttcacacctttacgcagatggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAAc                                                                                                                                              >  1:268441‑M1/150‑251 (MQ=255)                                                                                                         gagaacaccacatcatcggcgttcagttcacgcgtcggtttgaattctttattgtcgtgccacttcacacctttacgcagatggaaggtataggttttaccgtcttcgctgccttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGc                                                                                                                                           <  2:402567‑M1/105‑1 (MQ=255)                                                                                                         aaGGGCTATTTCCCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGc                                                                                                                                           <  2:115685/250‑1 (MQ=255)                                                                                                                    atcatcggcgttcagttcacgcgtcggtttgtattctttattgtcgtgccacatcacacctatactcagctggaaggtataggttttaacgtcttccctgacttcccacttttcagaggggcccgggatacccgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGCGAGGAAACTCTCAGTCAGAGGTGACTCACATGACAAAGACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGa                                                                                                                                 <  1:333777‑M1/115‑1 (MQ=255)                                                                                                                    atcatcggcgttcagttcacgcgtcggtttgaattctttattgtcgtgccacttcacccctttccgcagatggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGCCTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGa                                                                                                                                 <  2:134857‑M1/115‑1 (MQ=255)                                                                                                                       atcggcgttcagttcacgcgtcggtttgaattctttattgtcgtgccacttcacacctttacgcagatggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGATGTGACTCTCATGACAAAATCATTATCAAC‑CAGTAAAAAGCCCCGTAAACAGCATTCGCCTGAAttt                                                                                                                             >  2:57583‑M1/133‑251 (MQ=255)                                                                                                                            ggCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAACGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGc                                                                                                                          >  1:231718/1‑249 (MQ=255)                                                                                                                                 agttcacgcgtcggtttgaattctttattgtcgtgccacgtcacacctttacgcagatggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAGCAGCATTCGCCTGAATTTCGCAGTGAAg                                                                                                                   >  1:402567‑M1/123‑251 (MQ=255)                                                                                                                                    cTTGGAGCAAGCCTGATGTGTGAACAGTAGTATGGCGATGAAGTCGGGATGTTTGGTAAAGATAAGAGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACATATACGCCAGTGCCTCTTCCCCAGCAATAGTGGACACGCGGCTAAGTGAGTACACTCTTAGTCAGCGGTGAATCACATGACAAAAACAGTAT‑AACTCAGTAAAAAAACACGTAAACAGCAGTCGCCAGCATTTCGCAGCGACGccc                                                                                                                >  2:151717/1‑251 (MQ=21)                                                                                                                                     cacgcgtcggtttgaattctttattgtcgtgccacttcacacctttacgcagatggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCt                                                                                                               <  1:231583‑M1/133‑1 (MQ=255)                                                                                                                                      acgcgtcggtttgaattctttattgtcgtgccacttcacacctttacgcagatggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTg                                                                                                              <  1:151088‑M1/134‑1 (MQ=255)                                                                                                                                         cgtcggtttgaattctttattgtcgtgccacttcacacctttacgcagatggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGGGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGAATTCGCCTGAATTTCGCAGTGAAGCcctta                                                                                                             <  2:264135‑M1/135‑3 (MQ=255)                                                                                                                                            aaGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCtt                                                                                                        <  2:87033/251‑1 (MQ=255)                                                                                                                                                  gatggatgAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTg                                                                                                    <  2:19549/249‑1 (MQ=255)                                                                                                                                                  gaattctttattgtcgtgccacttcacacctttacgcagatggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGa                                                                                                   <  1:154743‑M1/145‑1 (MQ=255)                                                                                                                                                                   atGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGc                                                                                 >  2:239899/1‑251 (MQ=255)                                                                                                                                                                        gATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAg                                                                            <  1:280860/251‑1 (MQ=255)                                                                                                                                                                          cacacctttacgcagatggaaggcataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGcc                                                                          <  2:227625‑M1/170‑1 (MQ=255)                                                                                                                                                                           gAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTAACCCAGCAATAGTGGACACGCGTCTAAGTGAGTAAACTCTCAGTCAGAGGGGACTCACATGACAAAAACATTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGATCGCATCGGTGTTACTGCCGCAGccc                                                                         <  2:231718/251‑1 (MQ=255)                                                                                                                                                                                 gggCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAAc                                                                   >  1:281106/1‑251 (MQ=255)                                                                                                                                                                                   gCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTCTTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTc                                                                 >  2:334695/1‑251 (MQ=255)                                                                                                                                                                                     tGTTTGGTAAAGCTGCGCGCCAGAAAGCCCCGCATGCTATTTCTGCCGAGGTGACCTATCAGCCAGTGCCTCATACCCAGCAATAGTGGacac                                                                                                                                                                                                                              >  2:304008/1‑93 (MQ=255)                                                                                                                                                                                     tGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATGGCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGacac                                                                                                                                                                                                                              <  1:304008/93‑1 (MQ=255)                                                                                                                                                                                     gcagatggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTc                                                                 <  1:455386‑M1/179‑1 (MQ=255)                                                                                                                                                                                        gatggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAGCTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCt                                                            <  1:296428‑M1/184‑1 (MQ=255)                                                                                                                                                                                           ggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGt                                                          >  1:73496‑M1/65‑250 (MQ=255)                                                                                                                                                                                           ggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGACTGt                                                          <  1:254248‑M1/186‑1 (MQ=255)                                                                                                                                                                                                    aggttttaccgtcttcgctgacttaccccttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTACGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTCAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATcac                                                 <  2:286529‑M1/195‑1 (MQ=255)                                                                                                                                                                                                       gcCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAAct                                             >  1:136694/1‑251 (MQ=255)                                                                                                                                                                                                           accgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATGCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTAc                                         >  1:286529‑M1/49‑251 (MQ=255)                                                                                                                                                                                                                   cgcttacttcccacttgtcagcgaggcccgggatcacttaTCTTACCCAGCAATAGTGGACACGCGTCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGGGAAGCCCTGAATCTTGCTGAACGCATCGGTGTTTCTGCCGCAGCCCGTGAACTCAGCTTGTATTAACCTCAACTATACAACTGgc                                  >  2:308569‑M1/41‑250 (MQ=255)                                                                                                                                                                                                                     ctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGCATCACAACTCTACAACTGgcgc                                <  1:276699‑M1/212‑1 (MQ=255)                                                                                                                                                                                                                           tcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTaa                           >  1:389120‑M1/33‑249 (MQ=255)                                                                                                                                                                                                                           tcccacttttcagagagacccgggatcactgaTCTTACCCAGCAATAGTGGTCACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACACCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCAGTCAACTCAGCCTGTATGAATAAAAACTCTACAACTGGCGCAGGAAAc                         <  2:314360‑M1/219‑1 (MQ=255)                                                                                                                                                                                                                                    gAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATc                >  1:410241/1‑251 (MQ=40)                                                                                                                                                                                                                                       gTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCCGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATcagc             >  1:429055/1‑251 (MQ=38)                                                                                                                                                                                                                                         gaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATcagc             <  2:446719‑M1/231‑1 (MQ=255)                                                                                                                                                                                                                                               cgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGtct     <  1:371972‑M1/239‑1 (MQ=255)                                                                                                                                                                                                                                                 cGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGGGGGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGGATCAAC‑CAGTAAAAAACCCCGTAAACAGCAGTCGCCTGAATTTCGCAGTGAAGCCCAGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATAAATCACAACTCTACAACTGGCGCAGTAAACGGCAAAATCAGCAGACGtcttc   <  2:371852/251‑1 (MQ=11)                                                                                                                                                                                                                                                  gCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCCGACGtcttct  >  2:384248/1‑251 (MQ=18)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   TGAAACTGAGCGCCAATGGTTATATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAC‑CAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGTCTTCT  >  NC_000913/314979‑315470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.