Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,312,070	Δ1 bp	intergenic (‑28/+31)	alsB ← / ← alsR	D‑allose ABC transporter periplasmic binding protein/DNA‑binding transcriptional repressor AlsR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,312,069	0	G	.	100.0%	124.2 / NA	26	intergenic (‑27/+32)	alsB/alsR	D‑allose ABC transporter periplasmic binding protein/DNA‑binding transcriptional repressor AlsR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (9/17);  total (9/17)

AAAATCGCCTTCTGAAGGAGAGGCAAAAATATCAACGCTGACGCCCAGTGTTTTTGCTTCATCTTCAATGCCTTTTTTCATATCTACCCAAAATGGGTTGGAGAGGGTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTAT  >  NC_000913/4311839‑4312307                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                gacgaccaggttgcttttgcgacctgatgcgcaataggggcaaaccgtgacgacttttttcatcggtctcgctccagttactaaaatcacgcctacgcgctctcgactacagtatgcatcttttatgcaacattttatgtggggtcattcactgatattacgggcactatttattcaaaactctgacgaaaaccaggctgtcgtcagttttgtagtgaGGTAAGGGGACA‑GTAACTCCGCGATGGAGGAAt                                                                                                                                                                                                                            >  2:444600‑M2/219‑251 (MQ=255)        aggttgattttgcaacctgatgcgcaataggggcaaaccgtgacgacttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCttt                                                                                                                                                                                                                     >  2:196406‑M2/212‑251 (MQ=255)        aggttgattttgcaacctgatgcgcaataggggcaaaccgtgacgacttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcaggtttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCttt                                                                                                                                                                                                                     <  1:185747‑M2/40‑1 (MQ=255)         ggttgattttgcaacctgatgcgcaataggggcaaaccgtgacgacttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCtttt                                                                                                                                                                                                                    <  1:362697‑M2/41‑1 (MQ=255)            tgattttgcaacctgatgcgcaataggggcaaaccgtgacgacttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaacgctgacgaaaaacagtctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTc                                                                                                                                                                                                                  <  1:41742‑M2/43‑1 (MQ=255)              attttgcaacctgatgcgcaataggggcaaaccgtgacgacttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTa                                                                                                                                                                                                               <  1:122653‑M2/46‑1 (MQ=255)                         tgatgcgcaataggggcaaaccgtgacgacttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcgcgc                                                                                                                                                                                                    >  2:390614‑M2/195‑251 (MQ=255)                               gcaataggggcaaaccgtgacgacttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTg                                                                                                                                                                                              <  1:145767‑M2/63‑1 (MQ=255)                                                   cgacttttttcatcggtctcgctccagttaatcaaatcacgaatacgcgatctcgactacagtatgcatcttttatgccacactttatgaggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTACCTCAGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAACTCAACAATTgcg                                                                                                                                                                          >  2:424035‑M2/169‑251 (MQ=255)                                                           ttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGttt                                                                                                                                                                   <  1:390614‑M2/90‑1 (MQ=255)                                                              atcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggctctatttattcaaaacgctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAAGTACCTTTTGCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGgttttttc                                                                                                                                                               <  1:219304‑M2/94‑2 (MQ=255)                                                                 ggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttgtatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCat                                                                                                                                                            <  2:178583‑M2/97‑1 (MQ=255)                                                                      cgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactcggacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCatat                                                                                                                                                          <  2:118449‑M2/99‑1 (MQ=255)                                                                       gctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCatattaat                                                                                                                                                      <  1:170716‑M2/103‑1 (MQ=255)                                                                            agttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAg                                                                                                                                                 <  1:196406‑M2/108‑1 (MQ=255)                                                                               taatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccaccttttatgtggggtcattccctgatattacgggcactatttattcaaaactatgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCtt                                                                                                                                              >  1:237093‑M2/141‑251 (MQ=255)                                                                                                                                  ccattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAaggttg                                                                                           <  2:123820‑M2/162‑2 (MQ=255)                                                                                                                                     ttttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAggttggtt                                                                                        >  1:462536‑M2/87‑251 (MQ=255)                                                                                                                                              gggtaattccctgatattacgggcactatgtattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTGATTGCAGCGCGCCTGGTGAGAAGAAATCAACAAGTGCGCCGGTGTGTTCCATATTAAGAGTAGCTTGTTCAATTGTGAGCTGGGCGACAGAGACAAAAAAAGCTTCCAGCAAGGTT‑GTTGGTAATATTc                                                                              <  2:410384‑M2/174‑1 (MQ=255)                                                                                                                                                     tccctgatattacgggcactctttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTT‑GTAATATTCTTGCCg                                                                        >  2:242138‑M2/71‑250 (MQ=255)                                                                                                                                                                  gggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCt                                                          >  1:386875‑M2/58‑251 (MQ=255)                                                                                                                                                                      cctatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCtaata                                                      <  1:62335‑M2/198‑1 (MQ=255)                                                                                                                                                                         atttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACctaatat                                                     <  2:356397‑M2/199‑2 (MQ=255)                                                                                                                                                                                    actctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCggg                                        >  1:330683‑M2/40‑251 (MQ=255)                                                                                                                                                                                                                         gaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCt    <  1:119401‑M2/248‑1 (MQ=255)                                                                                                                                                                                                                          aGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTAt  <  2:152964‑M2/250‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                AAAATCGCCTTCTGAAGGAGAGGCAAAAATATCAACGCTGACGCCCAGTGTTTTTGCTTCATCTTCAATGCCTTTTTTCATATCTACCCAAAATGGGTTGGAGAGGGTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTAT  >  NC_000913/4311839‑4312307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑