Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,3630C.92.0% 93.9 / 2.5 25intergenic (‑490/+919)gatD/gatBgalactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base . (18/5);  total (18/7)
Fisher's exact test for biased strand distribution p-value = 7.00e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01
*NC_0009132,173,3640C.92.0% 93.8 / 2.5 25intergenic (‑491/+918)gatD/gatBgalactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base . (18/5);  total (18/7)
Fisher's exact test for biased strand distribution p-value = 7.00e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

AGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGA‑TTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTG‑ATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTC  >  NC_000913/2173122‑2173612
                                                                                                                                                                                                                                                    ||                                                                                                                                                                                                                                                        
aGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGA‑TTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTG‑ATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTc                                                                                                                                                                                                                                                >  1:154401/1‑251 (MQ=255)
      gcccgacAGTTGGGGGTGAAACCCAATAGTTGTCGTCGCGATCCAGAGGGTGAGGCTAATAATGATGACCCCCGATAT‑AAAGTGGGGGACAGAATTCCCACGGTGCGCGGCGACCGCCACCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAACCGGCAGCCCCTG‑ATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCcagca                                                                                                                                                                                                                                          <  2:19554/245‑1 (MQ=255)
       gccgccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGA‑TTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTG‑ATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCcagc                                                                                                                                                                                                                                           >  2:390604/1‑249 (MQ=255)
           ccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGA‑TTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTG‑ATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAAc                                                                                                                                                                                                                                     <  1:327406/251‑1 (MQ=255)
                            ccAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGA‑TTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTG‑ATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAAt                                                                                                                                                                                                                    >  1:401124/1‑251 (MQ=255)
                                        gTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGA‑TTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTG‑ATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTg                                                                                                                                                                                                        >  1:92954/1‑251 (MQ=255)
                                                   cccatctaaccacagaaTGAAGACACCCGAGATTAGGGTGGGGAAAGGA‑TGCCCAGGGTGCCGGGCGCCCGCCTCCGCCACGAAAAAGCGGAGGGGGGCAATACCGCAAAAGGGCAGCACCTG‑ATTCCCGGGCCCAAAAACACAAATTAAAATGG‑TGAGTGGAATAAAAATCGGTGTGGCCGATCCCACC‑‑GCCGTATGGCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTTACCGCCGAACt                                                                                                                                                                                             <  2:459079/236‑1 (MQ=255)
                                                          aTGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGA‑TTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTG‑ATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACt                                                                                                                                                                                             >  1:459079/1‑244 (MQ=255)
                                                           tGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGA‑TTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTG‑ATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGt                                                                                                                                                                                     >  1:145159/1‑251 (MQ=255)
                                                                                 gaTTAAGGTGCGGAACAGA‑TTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAAAGGCAGCACCTG‑ATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCtt                                                                                                                                                                 >  1:99033/1‑249 (MQ=255)
                                                                                     aaGGTGCGGAACAGA‑TTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAACCGGCAGCACCTG‑ATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGAAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAt                                                                                                                                                           >  1:433463/1‑251 (MQ=255)
                                                                                                                            ccccgcccagaagagGCCGAGGGTGGCAAAAGCGCCAACGGGCAGCAC‑TGTATTCCCCGGCCCACAAACAGCA‑TTAAAATGGGTGAGTGGAATAAAAACCGGGTTGGCCGAT‑CC‑CCCCGCCGTATGTCCCAGCAGCAGCGCCGGATCAAGGCCAATCAGGAACTCCGGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTg                                                                                                                     <  2:154401/242‑1 (MQ=255)
                                                                                                                                                  gtggCAAGATCGCCAAACGGCAGCACCTG‑ATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTgccgc                                                                                               >  1:318889/1‑251 (MQ=255)
                                                                                                                                                   gggaaaGACCGCCAACGGGCAGCCCCTG‑ATTCCCCGGCACACAAACAGCAATGAAAAGGG‑TGAGTGGGAAAAAAACCAGGTTTGCCGA‑GCC‑CGCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATTATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTgccgcc                                                                                              <  1:439426/247‑1 (MQ=255)
                                                                                                                                                                   cGGCAGCACCTG‑ATTACCCGGCACACACACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTag                                                                                                              >  1:195970/1‑219 (MQ=255)
                                                                                                                                                                              g‑ATTACTCGGCACAAAAACAGCGATTATCATGG‑TGACTGGCATACAGATCACCCTTGCCGATACAACC‑‑GCCCTATTTCACAGCAGCAACGACGGAGCAAAGCAAATCAGGAACTCCTGACCGCCGAACCTCGCCTGTAAACGACTACGAGCCAGCTAAGCCATGTGCGTTAAACCCTCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATAAATGACGCTGTTTTTACCGCCATCTGCAATAAACCt                                                                   >  2:290594/1‑251 (MQ=255)
                                                                                                                                                                                   aCCCGGCACACAAACAGCAATTAAAATGT‑TGAGTGGAAAAAAAAGCAGGCTTCCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAGCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTagcag                                                                                                           <  1:399868/207‑1 (MQ=255)
                                                                                                                                                                                   aCCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCCACGCCGGATCAAGGCCAATCAGGAACTCATGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTagcag                                                                                                           >  2:399868/1‑207 (MQ=255)
                                                                                                                                                                                      cGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAc                                                                                                                          >  1:119106/1‑189 (MQ=255)
                                                                                                                                                                                      cGGCACAAAAACAGCAATAAAAATGG‑GGAGGGGAAAAAAAATCAGCCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAc                                                                                                                          <  2:119106/189‑1 (MQ=255)
                                                                                                                                                                                         cacacaAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGt                                                         >  2:150607/1‑251 (MQ=255)
                                                                                                                                                                                                           aaTGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCg                                        >  1:165724/1‑250 (MQ=255)
                                                                                                                                                                                                               g‑TGAGTGGGATAAACATCAGGCTTGCCGATACCACA‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACCCCTTTGACATCGTAACCCGCGAGGATGCCGCTAAt                                   >  2:97628/1‑251 (MQ=255)
                                                                                                                                                                                                                               aTCAGGCTTGCCGATCCCATC‑‑GCCGTATGTCACAGCAGCACCGCTGGATCAAGGCCAATTAGGAACTCCTGACCGCCGACCTTCGCCTGTACACGCCTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGACCCATCACaa                     >  2:291468/1‑250 (MQ=255)
                                                                                                                                                                                                                                     cTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCAc              >  1:206124/1‑251 (MQ=255)
                                                                                                                                                                                                                                                   accgccgTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTc  >  1:245537/2‑251 (MQ=255)
                                                                                                                                                                                                                                                    ||                                                                                                                                                                                                                                                        
AGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGA‑TTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTG‑ATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTC  >  NC_000913/2173122‑2173612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑