Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	536,173	T→C	L155P (CTC→CCC)	hyi →	hydroxypyruvate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	536,173	0	T	C	100.0%	48.3 / NA	17	L155P (CTC→CCC)	hyi	hydroxypyruvate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/9);  total (8/9)

ATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTG  >  NC_000913/535935‑536416                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    aTTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTc                                                                                                                                                                                                                                          <  2:48539/250‑1 (MQ=255)                               ggCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAAGTAACGGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCGGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATgttg                                                                                                                                                                                                            <  2:23156/250‑1 (MQ=255)                                              aTTCGTTACGCCCGTGCGCTGGGCAATAAAAAAATTAACTGTCTGGTCGGTAACACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGa                                                                                                                                                                                             >  2:182389/1‑250 (MQ=255)                                                                          aaaaaaTTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGc                                                                                                                                                                  >  1:3408/1‑249 (MQ=255)                                                                                          ggTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAAt                                                                                                                                                <  1:238542/251‑1 (MQ=255)                                                                                                                       gTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGAt                                                                                                                   <  2:3408/251‑1 (MQ=255)                                                                                                                              gATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCAGCGAATACGCTGCTGTAAGAAGATATTTTATTCCTGATTGAACCTATTAACCCTTTTGATATTCCTGGTTTCAATCCCACCGGACCTCGGCCGGTGATGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCCGTGGGCTGATAAAATTg                                                                                                            >  1:177265/1‑251 (MQ=255)                                                                                                                                           cTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAACATTGGTCACCTGCAAAt                                                                                               >  2:90615/1‑251 (MQ=255)                                                                                                                                                                 ccGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCgcggc                                                                         >  2:218680/1‑251 (MQ=255)                                                                                                                                                                 ccGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCgcggc                                                                         <  1:218680/251‑1 (MQ=255)                                                                                                                                                                        tatGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGAATCGCGGCgaaccgg                                                                  >  2:196049/1‑251 (MQ=255)                                                                                                                                                                                     gaagaTATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAAGTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAaat                                                     <  1:203768/251‑1 (MQ=255)                                                                                                                                                                                                attacggagtgagcctgtgaaCCATTGTGATAGTCCTGGTTGCCATCCCACCGGAACTCGGCAGGCGGTGAAAGTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATGTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGGCACCTGCAAATTGCCGATAATCCGCCTCGCGGCGAACCGGGAACCGGAGAAAGTAATTATGAt                                           <  2:161924/231‑1 (MQ=255)                                                                                                                                                                                                   aCTGATTGAACCTATTAACCAGTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAAGTGATTGATGATGTTGGTTGCTGCAATTTGAAAAGGCATGATGACATTTATCATAGGCATCGGATGGAAGGTGAACTAACCAACACCATGTCTCAGGGGGCTGATAAAATTGGTCGGCTGCAAATTTCCGATAATCCGCATCGCAGCGAACCTGGATCCGTAGAAACTAATTATGATTAt                                        <  2:177265/250‑1 (MQ=255)                                                                                                                                                                                                          gAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGAAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGGGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCTGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTa                                 <  1:229436/250‑1 (MQ=255)                                                                                                                                                                                                                                ttCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTAc          >  1:131180/1‑251 (MQ=255)                                                                                                                                                                                                                                        ttCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACggttg  >  2:258961/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    ATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTG  >  NC_000913/535935‑536416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑