| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,032,423 | G→A | V219V (GTC→GTT) | yedJ ← | putative HD superfamily phosphohydrolase YedJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,032,423 | 0 | G | A | 96.8% | 99.2 / NA | 31 | V219V (GTC→GTT) | yedJ | putative HD superfamily phosphohydrolase YedJ |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (18/12); minor base C (0/1); total (18/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCA‑GCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACA‑TAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACC‑TTCATTCTCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAACGGCAAACACGCGCGCC > NC_000913/2032177‑2032669 | cgcgtgccAGTGCTTCTCACCTACACCATTAAGCTGCGCCAACAACGTTTTAACATCATAAAGTTGCAGCAGTATCTCCA‑GCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTGCTGATATATTTGCCTGCATAGATGTCACCGGCCATCTGAGAGGCCCGACAGGTTAACA‑TAACTACAGCCATTTAGGCCCACGCTCTCAGCCAGCGGAGGAGAACGCATCCATTACCGTGTTATCAacac < 2:295937/245‑1 (MQ=255) ccAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCA‑GCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACA‑TAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTc > 2:73674/1‑249 (MQ=255) ccAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCA‑GCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACA‑TAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTc < 1:73674/249‑1 (MQ=255) gCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCA‑GCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACA‑TAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCTTTCTCCCCC‑GCCAGTTCGGCACTGAGc < 1:85803/251‑1 (MQ=255) aaCATCATAAATTTGCAGCCGTTTC‑CCACGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATCTATTTTCCTGCATAGATTTCACCGGCCACCTGAGATGGCCGACAGGTTAACA‑TACTTACAGCCATTTCGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACt > 2:301809/1‑250 (MQ=255) catAAATTTGCAGCAGTTTCTCCA‑GCATTGCCTGGGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACA‑TAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCC‑GCCAGTTCGGCACGGAGCTTCGCCATAAACGCGACTaaaaaa < 1:261014/251‑1 (MQ=255) gcagcaGTTTCTCCA‑GCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACA‑TAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTtg > 1:32583/1‑251 (MQ=255) tttCTCCA‑GCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACA‑TAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTgcg > 1:283665/1‑251 (MQ=255) aTTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACA‑TAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCAGATGAAAACGCATCTATCACCTTGTGATCAACCCC‑TTCATTCTCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGttt < 1:32600/251‑1 (MQ=255) tCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACA‑TAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGc > 1:30578/1‑251 (MQ=255) cGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACA‑TAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCAGCTATCACCTTGTGATCAACCCC‑TTCATTCTCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATg < 1:59391/251‑1 (MQ=255) gTGCTGTATGAATCGGTTACTGATATTTTTTCCTGCATAGATTTCACGGGCCATCTGGGAGGGCCGACCGGTTAACA‑TATTTAAAGCCATTTAGGGCCACGCGCTCAGCAGGGGGATGAAAACGCATCTATCACCTTGTGATAAACACT‑TTCATTCTCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCGGCTGCTTGCCTCTTGCGGTTTGCATGGt < 1:83525/251‑1 (MQ=255) aCTGATATATTTTCCTGAATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACA‑TAATTACAGCCATTTAGGACCAAGAGCTCAGCCAGCGGATGAAAAAGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATACGCGTTGTGCTGCGCCAGCTGCGTGCCTCTTGCGGGTTGCATGGTTTGCGGTAGGTTCAGCa > 1:70986/1‑250 (MQ=255) agaGATTTCACCGGCCATCTGACATGGCCGACAG‑TTAACAATAATAACACCCATTTAGGGCCACCCTCCCAGCCAGGGGATGAAACCGCCCCTTTCACCTGGTGAGCAACCCC‑TCCATTCCCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCAt < 1:130544/210‑1 (MQ=255) aTAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTACCA‑TAATTACAGCCCTTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAACATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCAt > 2:130544/1‑212 (MQ=255) cGGCCATCTGAGATGGCCGACAGGTTAACA‑TAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGc > 2:243438/1‑251 (MQ=255) aTCTGAGATGGCCGACAGGTTAACA‑TAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCCTAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGttt > 2:55086/1‑250 (MQ=255) gggTTAAAA‑TAATTACGGCCTTTTAGGCCCACGCGCTCAGCCAGCGTATGAAACCCCACCTATCCCCTTG‑GATTCACACCCTCCATCCCCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGAt < 2:30578/250‑1 (MQ=255) ccAGCCATTTGGGGCCACGCGCACAGCCAGCGGATGAAACCTCATCTATCACCTTGGGATCAACACC‑TTCATTCTCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACgg < 1:221636/249‑1 (MQ=255) cAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACCCC‑TTCATTCTCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGAt < 1:101760/251‑1 (MQ=255) aTTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGCCTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTATCTTCAGCAACTTAGTCTGGAACTGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGGTGTGCGaccgtatcctcc > 2:29543/1‑240 (MQ=255) gggCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATc > 1:143397/1‑251 (MQ=255) ccACGCGCTCAGCCAGCGGATGAAAACGCATCTATCCACTTGTGATCAACACC‑TTCATTCTCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCaa > 1:20400/1‑250 (MQ=255) ccACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCaa > 2:94234/1‑250 (MQ=255) agccagcGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACg > 1:305722/1‑251 (MQ=255) agcGGATGAAAACGCATCTATCACCTTGTGCTCAACACC‑TTCATTCTCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGccc > 1:38188/1‑250 (MQ=255) ggtaggatgacgtgtcgaTCACCTAGAGATCAACACC‑TTCAG‑CTCCCCCCGGCAGTGCGGCACTTAGCGTCTCCCTATACTCGACGAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTGGTCTCTTGCGGTTTGCATTGTTTGCTGTAGCTTCAGCAACTTAGTCTGGAAAGGATCCAGCTCGTATCGTTTATCATCAAGCTGGGGATGGTGTGCGAACGGATCGTCTCCATCAAATAGCTCCACTCCCAAt < 2:130656/234‑1 (MQ=255) gAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGcccc > 1:15295/1‑251 (MQ=255) cGCATCTATCCCCTTGGGATCAACACC‑TTCATTCTCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGaaa < 1:55086/251‑1 (MQ=255) gCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGaacc > 2:244089/1‑249 (MQ=255) gATCAACACC‑TTCATTCTCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAACGGCAAACACGCGCGcc > 1:159537/1‑251 (MQ=255) | CGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCA‑GCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACA‑TAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACC‑TTCATTCTCCCCC‑GCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAACGGCAAACACGCGCGCC > NC_000913/2032177‑2032669 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |