| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,048,596 | G→A | G32G (GGC→GGT) | gfcC ← | capsule biosynthesis GfcC family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,048,596 | 0 | G | A | 96.3% | 97.3 / NA | 27 | G32G (GGC→GGT) | gfcC | capsule biosynthesis GfcC family protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (9/17); minor base T (0/1); total (9/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGCTGGCAATGAAATACGACTGTAATTTATTCATGGTGCGGGTTTAAGAAACGTCATTTCTACGGGAATAACCCCTGCGCCTAACATTTGCCGACTTTGACGAACCTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTGTTTTTC > NC_000913/1048368‑1048810 | gagTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATGGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGACATGACATGTTGGTAGGCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACGAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCAAGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCg > 1:285509/1‑251 (MQ=255)gagTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTGTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGTCAGATAAATAGTCAcc < 1:218362/250‑1 (MQ=255) tAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCAcc < 1:124222/247‑1 (MQ=255) tAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCAcc > 2:124222/1‑247 (MQ=255) aaCTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTc < 1:254441/250‑1 (MQ=255) cgcACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCaa > 2:234866/1‑250 (MQ=255) cgcACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCaa > 1:33012/1‑250 (MQ=255) caacgtaTTTAATCGTGGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAATCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAAGAGTCACCGTTCCTTGCGCAAAg < 2:128719/245‑1 (MQ=255) gtaTTTAATCGTTGCCGCCACATCGTCGTCAGCTTCGGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTGCCACGGGGCCATCGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCAt < 1:187227/249‑1 (MQ=255) tttAATCGTTGACGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGAGGTTGTGTCACCAACTGGACAACATTTTCCACGGCGCCAACGGCAAGGGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATggg > 2:111786/1‑251 (MQ=255) ttacacGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATggg < 2:57966/245‑1 (MQ=255) cgccACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCTCTTTAGCTTTCGCCGCAGAGTCGTGCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTAGTGTCACCAACTGGACAACATTTGCCTCGGGGCCAACGGAAAGGGTCTGTTGTTCACCATGCAGATAAGTAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGt < 1:198086/251‑1 (MQ=255) gccACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATATTGGTAGTCTTTAAGCGCTTTAGATTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGACACCAGAGGCGATCGCGCAGTTCCGGTTGTGTCCACAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAATCACCGTTCATTGCGCAAAGGAATGGGGTGTCATTACGt > 1:290842/1‑250 (MQ=255) cgtcgtCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTGGGTATTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGGCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTAc < 1:247926/250‑1 (MQ=255) gCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTCGGTATTCGTTAAGCGCTTTAGCTTTCGCCGCAGAGTCTGTCAGCAAGGCACCAGGCCACCAGAGGCGACCGCGCAGTTGCGGTTGTGGCGCCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGGTGTTCTCCAGGTAGATAAATAGTCACCGTTCCTTGCGCAAAGGCAGGGGCTGTCATTGCGTATTGCACGCTGGCAAt < 2:266350/251‑1 (MQ=255) gCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGCTGGCAATGAAAt < 1:20857/250‑1 (MQ=255) aGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCATCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTGGCGTTTGTGTCACCAACTGGACAATATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCGCCGTTCCTTGCGCAAAGGCATGGGGGGTCATTACGTAAAGTACGCTGGCAATGAAATACGACTGt < 1:206928/251‑1 (MQ=255) aGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTCCGCTGGCAATGAAATACGAcagt > 2:171569/1‑248 (MQ=255) caagcTGGGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGTGGTGTCATTACGTAAAGTACGCTGGCAATGAAGTACGACTGTAATTTa < 1:229873/251‑1 (MQ=255) gtgCCATGACAGGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGCTGGCAATGAAATACGACTGTAATTTATTCAt < 2:247367/250‑1 (MQ=255) gCCATGACCTGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGCTGGCAATGAAATACGACTGTAATTTATTCATggg > 1:252161/1‑250 (MQ=255) aTGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGGGTCATTACGTAAAGTACGCTGGCAATGAAATACGACTGTAATTTATTCCTGGTGCGGGttt > 2:277756/1‑251 (MQ=255) aGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGCTGGCAATGAAATACGACTGTAATTTATTCATGGTGCGGGTTTAAGAAAc < 2:11119/250‑1 (MQ=255) gCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGCTGGCAATGAAATACGACTGTAATTTATTCATGGTGCGGGTTTAAGAAACGTCATTTCTACGGGAATAACCCCTGCGCCTAACATTTGcc < 2:72571/251‑1 (MQ=255) agagGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGCTGGCAATGAAATACGACTGTAATTTATTCATGGTGCGGGTTTAAGAAACGTCATTTCTACGGGAATAACCCCTGCGCCTAACATTTGCCGACTTTGACGAACCTGGc < 1:209265/251‑1 (MQ=255) caacaTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGCTGGCAATGAAATACGACTGTAATTTATTCATGGTGCGGGTTTAAGAAACGTCATTTCTACGGGAATAACCCCTGCGCCTAACATTTGCCGACTTTGACGAACCTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTGat < 1:171569‑M1/251‑3 (MQ=255) aTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGCTGGCAATGAAATACGACTGTAATTTATTCATGGTGCGGGTTTAAGAAACGTCATTTCTACGGGAATAACCCCTGCGCCTAACATTTGCCGACTTTGACGAACCTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTGattgtt < 1:234866‑M1/251‑7 (MQ=255) | GAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGCTGGCAATGAAATACGACTGTAATTTATTCATGGTGCGGGTTTAAGAAACGTCATTTCTACGGGAATAACCCCTGCGCCTAACATTTGCCGACTTTGACGAACCTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTGTTTTTC > NC_000913/1048368‑1048810 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |