Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	670,635	A→T	L324Q (CTG→CAG)	holA ←	DNA polymerase III subunit delta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	670,635	0	A	T	100.0%	108.2 / NA	32	L324Q (CTG→CAG)	holA	DNA polymerase III subunit delta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (17/15);  total (17/15)

TTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCAGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGTTGTAATGTGCGCAACAAAATA  >  NC_000913/670403‑670874                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                ttACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACactgac                                                                                                                                                                                                                                <  2:253465/250‑1 (MQ=255)                                            gaACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAACTACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGCAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAgg                                                                                                                                                                                      >  2:146612/1‑249 (MQ=255)                                                          atgatTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGCTTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGAc                                                                                                                                                                      >  1:277914/1‑251 (MQ=255)                                                                gtgACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACt                                                                                                                                                                >  1:214367/1‑251 (MQ=255)                                                                              aCCAATCAAATTCGCCAGCGTTTCCACGGGTTATAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCGCTGCCCACACTGACTGGCCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAg                                                                                                                                                  <  1:199319/251‑1 (MQ=255)                                                                                                cGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGccc                                                                                                                                <  1:164540/251‑1 (MQ=255)                                                                                                           gTTTTAGATGACCATCGTGCACCGGATCATAGGTGCCGCCAAAGAGAGCCTGTAACGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCCTCATGCCCcg                                                                                                                     >  2:24375/1‑251 (MQ=255)                                                                                                                  aTGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTc                                                                                                              >  1:217105/1‑251 (MQ=255)                                                                                                                         tAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCAt                                                                                                        >  2:105035/1‑250 (MQ=255)                                                                                                                          aGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGCTACATACGTCAGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACCCTGACTGACCGTAATCTTGTTTGCGGGTGAGTTCCGTTCGTGTCAGGCGTTGCAAGGCCTGACGTACATGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGCTTCTGCAATAc                                                                                                      >  2:258622/1‑251 (MQ=255)                                                                                                                                   gATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGTTTCTGCCATACCCGATGCtt                                                                                             <  2:303601/251‑1 (MQ=255)                                                                                                                                       aaaGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGCCCg                                                                                                                                                                                                                          <  2:39885/122‑1 (MQ=255)                                                                                                                                       aaaGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCg                                                                                                                                                                                                                          >  1:39885/1‑122 (MQ=255)                                                                                                                                           gTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACAGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTCAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGAAGCTTATCCaac                                                                                      >  1:258207/1‑250 (MQ=255)                                                                                                                                              ccgccAAACAGAGCCTGTCACGCTTTCATATCCACCGTCGATAAATACGTACGCCAGGGGAATATGGCACCACGGAAGAGATAACCCTTCCTGATCTGCCCACACTGACTGACCGTACTCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCCCGGCCTGACGTAACTGCGTCTGACTTAAGCGCTTTAACGCCTCGCCCATCATGCCCCGGAGGGTTTGCCATAACCCATGCTTATCGaacaccc                                                                                  >  2:286995/1‑248 (MQ=255)                                                                                                                                                  cAAACAGTGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGTGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCTGGATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAacgcacg                                                                              <  1:155091/251‑1 (MQ=255)                                                                                                                                                          gCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCg                                                                      <  1:196281/251‑1 (MQ=255)                                                                                                                                                            ctggaacgATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCCTACCCGATGCTTATCAAACCAAGCACGCAGTGGCgac                                                                    >  2:10224/8‑249 (MQ=255)                                                                                                                                                              gTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTAt                                                                   <  1:194341/250‑1 (MQ=255)                                                                                                                                                                    aTTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGc                                                               >  1:285702/1‑248 (MQ=255)                                                                                                                                                                            tCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGc                                                                                                                                  >  1:304812/1‑173 (MQ=255)                                                                                                                                                                            tCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGc                                                                                                                                  <  2:304812/173‑1 (MQ=255)                                                                                                                                                                                    cGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGTCGTTTCAGGtt                                             <  2:149280/250‑1 (MQ=255)                                                                                                                                                                                          aTACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGt                                      >  1:301510/1‑251 (MQ=255)                                                                                                                                                                                                  gCCAGGTGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTTCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCATGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTTGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAg                              <  2:106436/251‑1 (MQ=255)                                                                                                                                                                                                   ccAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGt                             <  1:36823/251‑1 (MQ=255)                                                                                                                                                                                                   ccAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGt                             <  1:105035/251‑1 (MQ=255)                                                                                                                                                                                                    cAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATCCCCGATTCTTCTCAAACAACGCACGCCGTGGCGTATGGGCAGACTGGCGTTTCCGGTTAACCCGTAACAACAGtt                            >  1:174708/1‑251 (MQ=255)                                                                                                                                                                                                                    aacaGAAGAGCTAACCCTTCCTGCTCTGCCCACACTGACAGACCGTCATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTCACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGACGTGGCGTATGGGCAGACTGGCGTTTCAGGGTAAACCGTAACCACCGTTCACGTTGAAATGTgcc            >  2:274297/1‑250 (MQ=255)                                                                                                                                                                                                                            agaTAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGTTGTAATGTGCGCAACaaaa    <  2:214367/251‑1 (MQ=255)                                                                                                                                                                                                                              aTAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGGTTCAGGTTAACCAGTAACAACAGTTCACGTTGTAATGTGCGCAACAAAATa  <  1:177790/251‑1 (MQ=255)                                                                                                                                                                                                                              aTAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGGTTCAGGTTAACCAGTAACAAAAGTTCACGTTGTAATGTGCGACACAAAAt   >  1:248427/1‑250 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                TTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCAGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGTTGTAATGTGCGCAACAAAATA  >  NC_000913/670403‑670874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑