Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,640,045	+C	coding (13/534 nt)	rrrQ ←	putative lysozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,640,045	1	.	C	100.0%	123.0 / NA	34	I5V (ATC→GTC)	rrrQ	putative lysozyme
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (17/17);  total (17/17)

AGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGAT‑TTTTGTGTTCATAGATATTTATGATGAGGACGCTCGTGCTTATTGGCAGGATTTTCAATCTTAAAGGAGTACTGATGCTGCAGATAAGACTCAACTTTTTCTGACAATTTTTCTGCTACTTCCAGGAAGACTTGCCGGACGCTCCTTCTGGCTGCTGCCTCATAAAACTCCAGCGCAGCTCCTTCAACACGGTCCATGGCGACATCCAGGTCAAAAATTTCACCGTCAAAGCGTTCTTTGTC  >  NC_000913/1639801‑1640287                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   aGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTtg                                                                                                                                                                                                                                               >  2:79744/1‑251 (MQ=255)                  aaTGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCGTCCACCACGGTGGCGCCCCGACAGATGGTCCATATGCGAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGGCCAGAAACTGGTCAAGTATCTGATGATCAGATGCGCCTGCACCAATCAGCGCCAGAACTGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgctt                                                                                                                                                                                                                              <  1:125465‑M1/251‑12 (MQ=255)                   aTGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGGCGACAGGCCGTAGCTGATCTTTTGTGTTCAcccgcccgcttc                                                                                                                                                                                                                             <  1:95018‑M1/251‑13 (MQ=255)                        gTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGGCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGGATCTGATCTTTTGTGTTCAcccgcccgcttcgtctt                                                                                                                                                                                                                        <  2:217589‑M1/251‑18 (MQ=255)                            ccctGGTCGCATTTGTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCTCCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTGCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCGGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacga                                                                                                                                                                                                                    <  2:234297‑M1/250‑22 (MQ=255)                                 gTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattc                                                                                                                                                                                                               >  1:7584‑M1/1‑225 (MQ=255)                                   cGCATTTTTCCTGCGACAGTTTCATATTTGAAAAGACGGGTTTTCCATCCGCTACTGTGGCACCCCGCCAGATGGTCCATATGCCAGAACCATCGCTGTATGCCATGGTGTGGTTAACTGCTTTTTCGTCCAGAAACTGGTCAAGGATCTGAGGAGCAGATCCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTAAcccgcccgcttcgtcttacgatattca                                                                                                                                                                                                              <  2:5590‑M1/250‑28 (MQ=255)                                            ccTTCGACAATTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGAAAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCAACAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTACcccgcccgcttcgtcttacgatattcatcataaactt                                                                                                                                                                                                    >  2:18654‑M1/1‑214 (MQ=255)                                             cTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaacttt                                                                                                                                                                                                   >  2:228139‑M1/1‑213 (MQ=255)                                               tCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaacttt                                                                                                                                                                                                   <  1:303042‑M1/249‑39 (MQ=255)                                                    aGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCGGATCTTTTGTGTTCTcccgcccgcttcgtcttacgatattcatcataaactttgggatca                                                                                                                                                                                            <  1:287622‑M1/251‑46 (MQ=255)                                                                                        acTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgc                                                                                                                                                        >  1:253260‑M1/1‑170 (MQ=255)                                                                                               cACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgc                                                                                                                                                 >  1:10995‑M1/1‑163 (MQ=255)                                                                                                aCCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcg                                                                                                                                                >  2:248537‑M1/1‑162 (MQ=255)                                                                                                    cGACAGAGGGTCCAAATGCCAGAACCACCGCGGTATGCCATTGTGGGGTTTCCTTCCTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGGTGCGCCAGCCCCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTTTCCAcccgcccgcttcgtctgacgatattcatcataaactttgggatcatactgatgctccccgccagatgcctcctgtagacgcatcgcgcgacct                                                                                                                                            <  1:20834‑M1/251‑94 (MQ=255)                                                                                                     gACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgacctt                                                                                                                                           <  1:228139‑M1/251‑95 (MQ=255)                                                                                                                        aGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatccccttt                                                                                                                        >  1:467‑M1/1‑138 (MQ=255)                                                                                                                          aaCCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttcc                                                                                                                      <  1:248537‑M1/251‑116 (MQ=255)                                                                                                                                          cATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagc                                                                                                      <  1:109425‑M1/251‑132 (MQ=255)                                                                                                                                                    ttACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaa                                                                                                  <  1:11857‑M1/245‑136 (MQ=255)                                                                                                                                                    ttACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgacattcgggaactaaatcccctttccagctataaagcgacgccaaa                                                                                                  >  2:11857‑M1/1‑110 (MQ=255)                                                                                                                                                      aCCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCCGATGCGCCAGCACCAATCCGCGCCAGACCGGCAGCCGCAAGCCCGTATCTGATCTTTTGAGTTCAcccgcccgcctcgtattacgatattcatcataaactttgggctcatactgaagctccccgcccgatgcctcctgtagacgcctcgcgcgaccttcgggaactaactcccctttccagctataaagcgaagccaaacgaatacc                                                                                          >  1:280237‑M1/1‑108 (MQ=255)                                                                                                                                                             ttttCGTCCAGAAACTGGTCAAGTAGCTGAGGAGCAGAGGCGCCAGCCCCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgc                                                                                    <  2:253260‑M1/250‑150 (MQ=255)                                                                                                                                                                   tCCAGAACCTGGTCATGTATCTGCCGAGAAGATGCGCCAGCACCAATCCGCGCCAGAACGACAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatcttcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccatttccagctataaagcgaagccaaacgaatacctgctgattgtgc                                                                              >  1:100765‑M1/1‑95 (MQ=255)                                                                                                                                                                        aaaCTGGTCTAGAATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAAAGGCAGCCGACAGGCCGTATCTGATCTTTTGCGTTCAcccgcccgcttcgtcttaagatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaacagaatacctgctgcttgggccagttt                                                                        >  2:23576‑M1/1‑90 (MQ=255)                                                                                                                                                                            tGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtt                                                                    >  1:47184‑M1/1‑86 (MQ=255)                                                                                                                                                                                       cTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGCACGGCAGCCGACAGGACGTATCTGATCTTTTGTGTTCAcacgcccacttcgtcttccgatattaatcattaccttagggatcatcctgaagctacccgccagacgcctcctgtagacgcatagcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccga                                                          >  1:281403‑M1/1‑75 (MQ=255)                                                                                                                                                                                         gaggagCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaat                                                       >  1:94227‑M1/1‑73 (MQ=255)                                                                                                                                                                                                      gcCAGCACCAATCAGCACCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtc                                          >  2:2626‑M1/1‑60 (MQ=255)                                                                                                                                                                                                        cAGCCCCAAACAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcagcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttttgcaagttgtgtttttgaaccgaaatacaaaagagcgtcag                                        <  2:280237‑M1/251‑194 (MQ=255)                                                                                                                                                                                                                               gCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaacgttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagttttaagcatttaaaacaccttt                 <  1:284362‑M1/251‑217 (MQ=255)                                                                                                                                                                                                                                  gCCGACAGGCCGTATCTGATCTTTTGTGTTAAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagttttaagcatttaaaacccctttatt              >  2:286975‑M1/1‑32 (MQ=255)                                                                                                                                                                                                                                            cGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagttttaagcatttaaaacacctttatggtgagtcata    <  1:79744‑M1/251‑230 (MQ=255)                                                                                                                                                                                                                                               aTCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcaggtttaagcatttaaaacacctttattgttagtcataac  <  1:286975‑M1/250‑232 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   AGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGAT‑TTTTGTGTTCATAGATATTTATGATGAGGACGCTCGTGCTTATTGGCAGGATTTTCAATCTTAAAGGAGTACTGATGCTGCAGATAAGACTCAACTTTTTCTGACAATTTTTCTGCTACTTCCAGGAAGACTTGCCGGACGCTCCTTCTGGCTGCTGCCTCATAAAACTCCAGCGCAGCTCCTTCAACACGGTCCATGGCGACATCCAGGTCAAAAATTTCACCGTCAAAGCGTTCTTTGTC  >  NC_000913/1639801‑1640287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑