Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,312,219 A→G L258P (CTA→CCA)  alsR ← DNA‑binding transcriptional repressor AlsR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,312,2190AG96.4% 95.8 / ‑6.5 29L258P (CTA→CCA) alsRDNA‑binding transcriptional repressor AlsR
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (14/13);  total (15/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAG‑TTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATC  >  NC_000913/4311987‑4312452
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          
tccctgatattacgggcactatttattcaaaactctgacgacaaacaggctgtcgtcagttttgacgtgcGGTAAGGGGAC‑CGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGG‑TTGTAATATTCTTGCCg                                                                                                                                                                                                                         >  2:371549‑M2/71‑250 (MQ=255)
                           caaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGATACAAAAAAAGCGTCCAGCAAGGTTGG‑TTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTc                                                                                                                                                                                             <  1:267192‑M2/208‑1 (MQ=255)
                            aaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCTCGATGGCGGAATTACCTTTTTCTTAATTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTGTTTCCATATTAATATTAGCTTGGTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGG‑TTGTAATATTCTTGCCGAGGCATTACGACCTAGTAACGTCGTTTcc                                                                                                                                                                                            <  2:35258‑M2/209‑1 (MQ=255)
                                      cgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGG‑TTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTg                                                                                                                                                                                  >  2:110669‑M2/33‑251 (MQ=255)
                                          aaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGG‑TTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGc                                                                                                                                                                               >  1:457063‑M2/29‑250 (MQ=255)
                                                               gacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGG‑TTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGttt                                                                                                                                                         >  1:13810‑M2/8‑251 (MQ=255)
                                                                                           gcgATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGG‑TTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGt                                                                                                                              <  2:13810/251‑1 (MQ=255)
                                                                                             gATGGAGGAATTACCTTTTTCTTAATTCAGCGCGCCTGGTGCGAAGAAATCAACAATTGCGCCGGTTTTTTCAATATTACTATTAGCTTGTTCACTGTTGAGCTGGGCGACAGAGACCAAAAAAGCGTCCAGCAAGGTTGG‑TTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATTTGATCGGCCAGTTTCGCTATCGGTGAATGGGAGCTACGGGtt                                                                                                                             >  2:466318/1‑250 (MQ=255)
                                                                                              aTGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGG‑TTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTtat                                                                                                                           >  2:367507/1‑251 (MQ=255)
                                                                                                     aaTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGG‑TTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAACTAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAaat                                                                                                                     >  2:129249/1‑250 (MQ=255)
                                                                                                               ttCTTATTTCAGCGCGCCTGGTGAGAAGAACTCAACAATTGCGCCGGTTTTTTCCCTATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGG‑TTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATc                                                                                                                                                  >  2:313102/1‑211 (MQ=255)
                                                                                                               ttCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGG‑TTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATc                                                                                                                                                  <  1:313102/211‑1 (MQ=255)
                                                                                                                         aGCGCGCCTGGTGAGAAGAAATCATCAATTGCGCCGGGTTGTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGAGCGACAGAGACAAAAAAATCGTCCAGCAAGGTTGG‑TTGTAATATTCTTGCCGAGGCATTACGTCCTAATATCGGCGGTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATTGGTTATACAAATAATCTTGGCCCCGTGCttttt                                                                                                <  2:264452/251‑1 (MQ=255)
                                                                                                                                  ggTGAGAAGCAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGG‑TTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGcccc                                                                                                         >  2:196636/1‑233 (MQ=255)
                                                                                                                                  ggTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGG‑TTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGcccc                                                                                                         <  1:196636/233‑1 (MQ=255)
                                                                                                                                      agaagaAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGG‑TTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACg                                                                                   <  2:154730/251‑1 (MQ=255)
                                                                                                                                                        cgcCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGG‑TTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTg                                                                 >  1:120811/1‑251 (MQ=255)
                                                                                                                                                         gcCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGG‑TTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTgg                                                                <  1:119315/251‑1 (MQ=255)
                                                                                                                                                            ggTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGG‑TTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTc                                                             >  1:160749/1‑251 (MQ=255)
                                                                                                                                                                     cATATTAATATTAGCTTGTTCAATGTTGATCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTTG‑TTGTAATATTCTGGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAAt                                                    <  1:466318/251‑1 (MQ=255)
                                                                                                                                                                      atattaatattaGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGG‑TTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATg                                                   >  1:257839/1‑251 (MQ=255)
                                                                                                                                                                                 aGCTTGTTCAATCTTGCACTGGACGGCAGCGACAAACACAGCGTCAAG‑AAAGGAAGGTTGTAATATTCTTACTGAGGCATTACGACCTAATAACGGCGCTTCCGGGGCTGGTGAGCAAATAATATGCTCGGCCAGTTAAGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAACCTTTGCCCCGTTCTTTTTTGCCAGTTCCACGGCTGCTTTTCCATCACTGGTTTGCCAGGCATGGGTCCCTAccc                                        >  2:209552/1‑250 (MQ=255)
                                                                                                                                                                                    ttGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGG‑TTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCTCATTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGc                                      >  2:253719/1‑250 (MQ=255)
                                                                                                                                                                                           aTGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGG‑TTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACAtc                              <  2:177755/251‑1 (MQ=255)
                                                                                                                                                                                                    ggtgCGACAGAGACAAAAAAAGCGGCCAGCAAGGGTGG‑TTGTAATATTCTTGCCGATGCATTACTGCCTAATAACGGCGTTGCCGGGGCTGGTGAGCAAATAATATGAACGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGGTCTACGGCCGCGTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAGCAACTCCTTCCTg                     <  2:212695/248‑1 (MQ=255)
                                                                                                                                                                                                           cAGAGACAAAAAAAGCGTCCAGCAAGGTTGG‑TTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTaacaac              <  1:342272/251‑1 (MQ=255)
                                                                                                                                                                                                                aCAAAATAAGGGTCCAGCAAGGTTGG‑TTGTAATAATCTTGCCGAGGCATTACGACCTAATACCGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAAGGGTTCACTACCAGCACATCATCTCCTTCCCGTAACAACGAAGc         <  2:11351/251‑1 (MQ=255)
                                                                                                                                                                                                                 cAAAAAAAGCGTCCAGCAAGGTTGG‑TTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCg        <  1:110669/251‑1 (MQ=255)
                                                                                                                                                                                                                        aGCGTCCAGCAAGGTTGG‑TTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAcatc  >  1:8321/1‑250 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          
GTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAG‑TTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATC  >  NC_000913/4311987‑4312452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑