Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,235,651 T→C intergenic (+244/‑255) pgi → / → yjbE glucose‑6‑phosphate isomerase/uncharacterized protein YjbE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,235,6510TC100.0% 62.8 / NA 20intergenic (+244/‑255)pgi/yjbEglucose‑6‑phosphate isomerase/uncharacterized protein YjbE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/11);  total (9/11)

AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAAT  >  NC_000913/4235406‑4235876
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                 
aaTCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTa                                                                                                                                                                                                                               <  1:338358/250‑1 (MQ=255)
         cgATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGAtt                                                                                                                                                                                                                      <  1:426160/250‑1 (MQ=255)
                     cGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTGTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCgttgtt                                                                                                                                                                                                         <  2:210526/251‑1 (MQ=255)
                        aTAAGGCGTTCACGCAGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTAt                                                                                                                                                                                                      <  2:96104/251‑1 (MQ=255)
                          aaGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTAtt                                                                                                                                                                                                     <  2:69048/250‑1 (MQ=255)
                                                              ggCGACGCGGTCGCGTATTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCGTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTttttatt                                                                                                                                                                <  2:150817/250‑1 (MQ=255)
                                                                                          aCAGGTCGATGCCGTTATGGACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTCATGTCGGCATTTTGTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATAGCGTGAGGGTAACCCTAAAAGAGATTTTTAATCGTTGTTTATTTCGGAAAATACTCAGATTAATTGCTTTTGTTTTTATGTTAAGTTTATGATTTTTAGTGTTAttt                                                                                                                                     <  2:399485/250‑1 (MQ=255)
                                                                                                 gATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAAtata                                                                                                                              >  2:295473/1‑250 (MQ=255)
                                                                                                     ccGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCAGTTTGTTATCAGATAAATCCCCTTGTCTGTAAGTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTGTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTGATTTAAATATAAGTTg                                                                                                                         <  2:369791/251‑1 (MQ=255)
                                                                                                        atatGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCttt                                                                                                                                                                          <  2:167266/199‑1 (MQ=255)
                                                                                                        atatGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCttt                                                                                                                                                                          >  1:167266/1‑199 (MQ=255)
                                                                                                                                          aCGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTaaa                                                                                     >  1:371366/1‑250 (MQ=255)
                                                                                                                                            gattgattAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAACTCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTaaaccg                                                                                  >  1:256357/1‑248 (MQ=255)
                                                                                                                                                gattAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCAt                                                                              >  1:109528/1‑251 (MQ=255)
                                                                                                                                                   tAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGaaaa                                                                                                                                                                                             >  1:92157/1‑137 (MQ=255)
                                                                                                                                                   tAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGaaaa                                                                                                                                                                                             <  2:92157/137‑1 (MQ=255)
                                                                                                                                                               ataaCAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGCTTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCcgcg                                                               >  2:206450/1‑251 (MQ=255)
                                                                                                                                                                        ccGACGTTATGTCGGCATTGTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTc                                                      <  2:2109/251‑1 (MQ=255)
                                                                                                                                                                                              ttATCAGCTAAATCCCCATGACTGTCATTTAACGGGAATCATACAGTGAGGTTCACCCTAAAATAGATTTTTAATGGCTGTTTATTTAGAAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTAATTTAACAATAATTTGAAAATTATATTGGATTTTAATTCAATTTATCCTAAAACGCCATCGCTAATTCCCCGCCCCGTAATTCGCATGTTTTATTTGTTTATACt                                >  2:274973/1‑251 (MQ=255)
                                                                                                                                                                                                                            aaCGGAAATCACACCGTGAGGATAACCCTATAATAGATTTTTAATCGTTGTTTACATCCGAACATAAGCAGCTTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTACATATAAGTTGATACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTACCCGCGCCGTCATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAAt  >  2:414711/1‑251 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                 
AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAAT  >  NC_000913/4235406‑4235876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑