Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,158,172	G→T	A175S (GCC→TCC)	yqhG →	DUF3828 domain‑containing protein YqhG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,158,172	0	G	T	100.0%	116.0 / NA	35	A175S (GCC→TCC)	yqhG	DUF3828 domain‑containing protein YqhG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/17);  total (18/17)

TAGAAAGCGTTGCGATAACCCAAACTGACGCCGATCATGCCGATGCCGTTGTGCGCTTTCGTATCTTTAAAGATGATAAAGAAAAGACCACGCAGACACTGAAAATGGTGGCGGAAAATGGTCGTTGGGTCATTGACGATATTGTCAGCAATCATGGCAGCGTCTTACAAGCAGTTAATAGCGAGAATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTTGCCGAACTCTTTGAACATATTGCTGATTATAGCTGGCCGTGGACGTGGGTGGTTTCCGACTCTTACCGCCAGGCGGTTAATGCCTTCTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCGTTGATGAAATTCGAGTCCTGGAGAAAACC  >  NC_000913/3157927‑3158399                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                    tAGAAAGCGTTGCGATAACCCAAACTGACGCCGATCATGCCGATGCCGTTGTGCGCTTTCGTATCTTTAAAGATGATAAAGAAAAGACCACGCAGACACTGAAAATGGTGGCGGAAAATGGTCGTTGGGTCATTGACGATATTGTCAGCAATCATGGCAGCGTCTTACAAGCAGTTAATAGCGAGAATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAATCCTTTGTTTCCGa                                                                                                                                                                                                                                 >  2:266543/1‑250 (MQ=255)     aaGCGATGCGATACCCCAAACTGACGCCGATCATGCCGATGCCGTTGTGCGCTTTCGTATCTTTAAAGATGATAAAGAAAAGACCACGCAGACACTGAAAATGGTGGCGGCAAATGGTCGTTGGGTCATTGACGATATTGTCCGCAATCATGGCAGCGTCTTACAAGCAGTTAATAGCGAGAATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTTTCCGAActct                                                                                                                                                                                                                            >  2:415287/1‑251 (MQ=255)        cGTTGCGATAACCCAAACTGTCGCCGATCATGCCGATGCCGTTGTGCGCTTTCGTATCTTTAAAGATGATAAAGAAAAGCCCACGCAGACACTGAAAATGGTGGCGGAAAATGGTAGTTGGGTCATTGACGATATTGTCAGCAATCATGGCAGCGTCTTACAATCAGTTAATAGCGAGAATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCCAAAAGAACAGCCGGAAGCCTTTGTTTCCGAACTCtttt                                                                                                                                                                                                                         >  2:463057/1‑250 (MQ=255)        cGTTGCGATAACCCAAACTGACGCCGATCCTGCCGATGCCGTTGAGCGCCTTCGTATCTTTAAAGATGATAAAGAAAAGACCAAGCAGACACTGAAAATGGTGGCGGAATATGGTCGTTGGGTCATTGAAGATATTGTCAGCAATCATGGCAGCGTCTTACAAGCAGTTAATAGCGAGAATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAACCCTTTGTTTCCGAACTCTTTg                                                                                                                                                                                                                         >  1:448684/1‑251 (MQ=255)                   cccAAACTGACGCCGATCATGCCGATGCCGTTGTGCGCTTTCGTATCTTTAAAGATGATAAAGAAAAGACCACGCAGACACTGAAAATGGTGGCGGAAAATGGTCGTTGGGTCATTGACGATATTGTCAGCAATCATGGCAGCGTCGTACAAGCAGTTAATAGCGAGAATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGTAAAAAGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAACATATTGCt                                                                                                                                                                                                              <  1:445125/251‑1 (MQ=255)                     cAAACTGACGCCGATCATGCCGATGCCGTTGTGCGCTTTCGTATCTTTAAAGATGATAAAGAAAAGACCACGCAGACACTGAAAATGGTGGCGGAAAATGGTCGTTGGGTCATTGACGATATTGTCAGCAATCATGGCAGCGTCTTACAAGCAGGTAATAGCGAGAATGAAAAAGCGCTGGGTGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAACATATTGCTGa                                                                                                                                                                                                            <  1:454420/251‑1 (MQ=255)                       aaCTGACGCCGATCATGCCGATGCCGTTGTGCGCTTTCGTATCTTTAAAGATGATAAAGAAAAGATCACGCAGACACTGAAAATGGTGGCGGAAAATGGTCGTTGGGTCATTGACGATATTGTCAGCAATCATGGCAGCGTCTTACAAGCAGTTAATAGCGAGAATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAACATATTGCTGAtt                                                                                                                                                                                                          <  2:106254/251‑1 (MQ=255)                       aaCTGACGCCGATCATGCCGATGCCGTTGTGCGCTTTCGTATCTTTAAAGATGATAAAGAAAAGACCACGCAGACACTGAAAATGGTGGCGGAAAATGGTCGTTGGGTCATTGACGATATTGTCAGCAATCATGGCAGCGTCTTACAAGCAGTTAATAGCGAGTATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTGTCCGAACTCTTTGAACATATTGCTGAtt                                                                                                                                                                                                          >  2:352081/1‑251 (MQ=255)                               ccGATCATGCCGATGCCGTTGTGCGCTTTCGTATCTTTAAAGATGATAAAGAAAAGACCACGCAGACACTGAAAATGGTGGCGGAAAATGGTCGTTGGGTCATTGACGATATTGTCAGCAATCATGGCAGCGTCTTACAAGCAGTTAATAGCGAGAATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAACATATTGCTGATTATAGCTgg                                                                                                                                                                                                  <  1:58142/251‑1 (MQ=255)                                           atgccgTTGTGCGCTTTCGTATCTTTAAAGATGATAAAGAAAAGACCACGCAGACACTGAAAATGGTGGCGGAAAATGGTCGTTGGGTCATTGACGATATTGTCAGCAATCATGGCAGCGTCTTACAAGCAGTTAATAGCGAGAATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAACATATTGCTGATTATAGCTGGCCGTGGACgtgg                                                                                                                                                                                      >  2:156511/1‑251 (MQ=255)                                                   gtgCGCTTTCGTATCTTGAAAGATGAAAAAGAAAAGACCACGCAGACACTGAAAATGGTGGCGGAAAATGGTCGTTGGGTCATTGACGATATTGTCAGCAATCATGGCAGCGTCTTACAAGCAGTTAATAGCGAGAATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAACATATTGCTGATTATAGCTGGCCGTGGACGTGGGTGGttt                                                                                                                                                                               <  2:85203/250‑1 (MQ=255)                                                                  tttAAAGATGATAAAGAAAAGACCACGCAGACACTGAAAATGGTGGCGGAAAATGGTCGTTGGGTCATTGACGATATTGTCAGCAATCATGGCAGCGTCTTACAAGCAGTTAATAGCGAGAATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAACATATTGCTGATTATAGCTGGCCGTGGACGTGGGGGGTTTCCGACTCTTACCGcc                                                                                                                                                                >  1:254745/1‑250 (MQ=255)                                                                         atgatAAAGAAAAGACCACGCAGACACTGCAAATGGCGGCGGAAAATGGTCGTTGGGTCATTGACGATATTGTCAGCAATCATGGCAGCGTCTTACAAGCAGTTAATAGCGAGAATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAACATATTGCTGATTATAGCTGGCCGTGGACGTGGGTGGTTTCCGACTCTTACCGCCAGGCGGtt                                                                                                                                                        >  1:156650/1‑251 (MQ=255)                                                                                  aaaagaCCCCGCAGACACTGACCATGGCGGCGGCAAATGGACGTTGGGTAATTGACGATATTGTCAGCAATCATGGCCGCGTCTTACAAGCAGTTAATAGCGAGAATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAACGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAATATCTTGCTGATTATAGCTGGCCGTTGACGTGGGTGGTTTCTGACTCTTACCGCCAGGCGGTTGATGTCTTc                                                                                                                                               >  2:439421/1‑251 (MQ=255)                                                                                   aaagaCCACGCAGACACTGAAAATGGTGGCGGAAAATGGTCGTTGGGTCATTGACGATATTGTCAGCAATCATGGCAGCGTCTTACAAGCAGTTAATAGCGAGAATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAACATATTGCTGATTATAGCTGGCCGTGGACGTGGGTGGTTTCCGACTCTTACCGCCAGGCGGTTAATGCCTTCt                                                                                                                                              >  2:367259/1‑251 (MQ=255)                                                                                      gaCCACGCAGACACTGAAAATGGTGGCGGAAAATGGTCGTTGGGTCATTGACGATATTGTCAGCAATCATGGCAGCGTCTTACAAGCAGTTAATAGCGAGAATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAACATATTGCTGATTATAGCTGGCCGTGGACGTGGGTGGTTTCCGACTCTTACCGCCAGGCGGTTAATGCCTTCtat                                                                                                                                            >  1:315779/1‑250 (MQ=255)                                                                                                 cacTGAAAATGGTGGCGGAAAATGGTCGTTGGGTCATTGACGATATTGTCAGCAATCATGGCAGCGTCTTACAAGCAGTTAATAGCGAGAATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAACATATGGCTTATTATAGCTGGCCGTGGACGTGGGTGGTTTCCGACTCTTGCCGCCAGGCGGTTAATGCCTTCTATAAAACCACCTTc                                                                                                                                <  1:210366/251‑1 (MQ=255)                                                                                                     gAAAATGGTTGCGGAAAATGTTCGTTGGGTCATTGACGATATTGTCAGCAATCATGGCAGCGTCTTACAAGCAGTTAATAGCGAGAATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGATGCCTTTGGTTCCGAACTCTTTGAACATATTGCTGATTATAGCTGGCCGTGGACGTGGGTGGTTTCCGACTCTTACCGCCAGGCGGTTAATGCCTTCTATAAAACCACCTTCAAg                                                                                                                             <  1:210581/250‑1 (MQ=255)                                                                                                                   aaaaTGGTCGTTGGGTCATTGACGATATTGTCAGCAATCATGGCAGCGTCTTACAAGCAGTTAATAGCGAGAATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAACATATTGCTGATTATAGCTGGCCGTGGACGTGGGTGGTTTCCGACTCTTACCGCCAGGCGGTTAATGCCTTCTATAAAACCACCTTCAAGACGGCCAATAATccc                                                                                                              <  2:208058/251‑1 (MQ=255)                                                                                                                     aatgggctttGGGTCATTGACGATATTGTCAGCAATCATGGCAGCGTCTTACAAGCAGTTAATAGCGAGAATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTTTCCGACCTCTTTGAACAGATTGCTGATTATAGCTGGCCTTGGACGTGGGTGGTGTCCGACTCTTACCGCCAGGCTGTTAATTCCTTCTATAAAACCACCTTCAAGACGGCCAATAATCCCGa                                                                                                            <  1:24406/243‑1 (MQ=255)                                                                                                                                   cATTGACGATATTGTCAGCAATCATGGCAGCGTCTTACAAGCAGTTAATAGCGAGAATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAACATATTGCTGATTATAGCTGGCCGTGGACGTGGGTGGTTTCCGACTCTTACCGCCAGGCGGTTAATGCATTCTATAAAACCACCTTCAAGACGGACAATAATCCCGATGAAGATATGCaaa                                                                                              <  1:98762/251‑1 (MQ=255)                                                                                                                                             atTGTCAGCAATCATGACAACGTGTTAGTAGCCGTTAATAGCGAGAATGAAGCAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGATTCCGAACTCTTTGAACATATTGCTGATTATAGCTGGCCGTGGACGTGGGAGGTTTCCGACTCTTACCGCCAGGAGGTTAATGCCTTCTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGTTATGCAAATAGAACGGcc                                                                                    >  2:293121/1‑250 (MQ=255)                                                                                                                                                                  gTCTTACAAGCAGTTAATAGCGAGAATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAACATATTGCTGATTATAGCTGGCCGTGGACGTGGGTGGTTTCCGACTCTTACCGCCAGGCGGTTAATGCCTTCTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGAt                                                               >  2:402883/1‑251 (MQ=255)                                                                                                                                                                       aCAAGCAGTTAATAGCGAGAATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAACATATTGCTGATTATAGCTGGCCGTGGACGTGGGTGGTTTCCGACTCTTACCGCCAGGCGGTTAATGCCTTCTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGtt                                                          <  1:402883/251‑1 (MQ=255)                                                                                                                                                                                 aaTAGCGAGAAGGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAACATATTGCTGATTATAGCTGGCCGTGGACGTGGGTGGTTTCCGACTCTTACCGCCAGGCGGTTAATGCCTTCTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAag                                                 <  1:60284/250‑1 (MQ=255)                                                                                                                                                                                    aGCGAGAATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAACATATTGCTGATTATAGCTGGCCGTGGACGTGGGTGGTTTCCGACTCTTACCGCCAGGCGGTTAATGCCTTCTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTc                                             <  1:181557/251‑1 (MQ=255)                                                                                                                                                                                     gCGAGAATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAACATATTGCTGATTATAGCTGGCCGTGGACGTGGGTGGTTTCCGACTCTTACCGCCAGGCGGTTAATGCCTTCTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCg                                            >  1:33046/1‑251 (MQ=255)                                                                                                                                                                                        agaATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAACATATTGCTGATTATAGCTGGCCGTGGACGTGGGTGGTTTCCGACTCTTACCGCCAGGCGGTTAATGCCTTCTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCt                                          >  1:347002/1‑250 (MQ=255)                                                                                                                                                                                         gaATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAACATCTTGCTGATTATAGCTGGCCGTGGACGTGGGTGGTTTCCGACTCTTACCGCCAGGCGGTTAATGCCTTCTCTAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATCTGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTAt                                        >  2:320687/1‑251 (MQ=255)                                                                                                                                                                                             aaaaaaaCGCGGGCCGCTTTAGCTGCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAACATATTGCTGATTATAGCTGGCCGTGGACGTGGGTGGTTTCCGACTCTTACCGCCAGGCGGTTAATGCCTTCTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTc                                    <  2:164043/250‑1 (MQ=255)                                                                                                                                                                                                 aaaCGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGCAGCCTTTGTTTCCGAACTCTTTGAACATATTGCTGATTATAGCTGGCCGTGGACGTGGGTGGTTTCCGACTCTTACCGCCAGGCGGTTAATGCCTTCTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTTTTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCAcgc                                >  1:297354/1‑251 (MQ=255)                                                                                                                                                                                                               ttAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAACATATTGCTGATTATAGCTGGCCGTGGACGTGGGTGGTTTCCGACTCTTACCGCCAGGCGGTTAATGCCTTCTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCGTTGATGAAATTc                   <  1:266543/250‑1 (MQ=255)                                                                                                                                                                                                                         tGCAAAAAGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAACATATTCCTGATTATAGCAAGCCGTGGACGTGGGTGGTTTACGACTCTTACCGCCAGGCGGTTAATGACTTCTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTCACGAAAATCCGATCTGTTTTGGCGACGAGTCGCTATTTTCACGCGTTGATGCACTTCGAGTACTgggg        >  1:46049/1‑249 (MQ=255)                                                                                                                                                                                                                               aaGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAACATATTGCTGATTATAGCTGGCCGTGGACGTGGGTGGTTTCCGACTCTTACCGCCAGGCGGTTAATGCCTTCTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCGTTGATGAAATTCGAGTCCTGGAGAAAAcc  <  1:458563/251‑1 (MQ=255)                                                                                                                                                                                                                               aaGAACAGCCGGAAGCCTTTGTTTCCGAACTCTTTGAACATATTGCTGATTATAGCTGGCCGTGGACGTGGGTGGTTTCCGACTCTTACCGCCAGGCGGTTAATGCCTTCTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCGTTGATGAAATTCGAGTCCTGGAGAAAAcc  <  1:367259/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                    TAGAAAGCGTTGCGATAACCCAAACTGACGCCGATCATGCCGATGCCGTTGTGCGCTTTCGTATCTTTAAAGATGATAAAGAAAAGACCACGCAGACACTGAAAATGGTGGCGGAAAATGGTCGTTGGGTCATTGACGATATTGTCAGCAATCATGGCAGCGTCTTACAAGCAGTTAATAGCGAGAATGAAAAAACGCTGGCCGCTTTAGCTTCGTTGCAAAAAGAACAGCCGGAAGCCTTTGTTGCCGAACTCTTTGAACATATTGCTGATTATAGCTGGCCGTGGACGTGGGTGGTTTCCGACTCTTACCGCCAGGCGGTTAATGCCTTCTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCGTTGATGAAATTCGAGTCCTGGAGAAAACC  >  NC_000913/3157927‑3158399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑