| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,032,423 | G→A | V219V (GTC→GTT) | yedJ ← | putative HD superfamily phosphohydrolase YedJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,032,423 | 0 | G | A | 97.4% | 135.3 / ‑4.9 | 38 | coding (657/696 nt) | yedJ | putative HD superfamily phosphohydrolase YedJ |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (23/14); minor base . (0/1); total (23/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATG‑GCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAACGGCAAACACGCGCGCC > NC_000913/2032188‑2032669 | gATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATG‑GCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTcc < 2:278342/251‑1 (MQ=255) cACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATAATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATG‑GCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTccccc < 1:389706/248‑1 (MQ=255) cacCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCTTCTGAGATG‑GCCGACAGTTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCCGCCAGTTCg < 1:439504/251‑1 (MQ=255) cccaACGTTTTAACATCATAAATTTGCAGCAGTTGCGCCAGCATTGCCTGTGCGGCATGCCCGGGGCTTGATGAAGCGGTTACTGATATATTTTCCGGCATAGATTTCACCGGCCATCTGAGATG‑GCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCTGCGGATGAAAACGCATCTATCACCTTGTTAGCAACGCC‑TTCATCCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCAt < 2:218120/250‑1 (MQ=255) cagcagTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATG‑GCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTtgt > 1:367647/1‑251 (MQ=255) aTTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATG‑GCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGttt > 2:85011/1‑251 (MQ=255) aTTCCCGGTGCTGTATGAATCGGTTACTGATATATTCTCCTGCATAGATTTCACCGGCCATCTGAGATG‑GCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGttt > 1:454130/1‑251 (MQ=255) gCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATG‑GCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAACAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGttt > 2:90669/1‑251 (MQ=255) attgaTCGGTTACTGATATATTTCCCGGCAAAGATTTCCCCGGCCATCTG‑GAAGTGCCGACAGGTTAAAATAATTACACCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCCCCTTGTGATCAACACC‑TTCATCCCCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGt < 2:327065/247‑1 (MQ=255) tGAATCGTTTACTGATATATTTCCCGCCATAGATTTCCCCGGCCATCTGAGATG‑GCCGACAGGTTAACATAATTACGGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCTTCTATCCCCTTGTGATCAACACC‑TTCATGCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGt < 2:132794/250‑1 (MQ=255) gAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATG‑GCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTa < 1:165860/250‑1 (MQ=255) aTCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATG‑GCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGt > 1:327065/1‑247 (MQ=255) gTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGTTG‑GCCGACAGGTTAACATACTTACAGCCATTTAGGGCCAAGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTc > 2:61882/1‑249 (MQ=255) cTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATG‑GCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAAc > 2:101298/1‑251 (MQ=255) gATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATG‑GCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGt > 2:299105/1‑237 (MQ=255) gATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATG‑GCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGt < 1:299105/237‑1 (MQ=255) gATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATG‑GCCAACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCCCCTTGTGATCAACACC‑TTCATTCCCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTGGCATGGTTTGCGGTAGCTTCAGCAAc < 1:366074/249‑1 (MQ=255) cgctcttcagacccccggcctccTGAGAGG‑GCCGACAGGTAAACAAAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAACCGCATCTACCACCTTGTGATCAACCCC‑TTCATCCCCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAAt < 2:101456/229‑1 (MQ=255) cATAGATTTCACCGGCCATCTGAGATG‑GCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTACAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCCGCCACTTAGTCTGGAAATGAt > 2:103446/1‑251 (MQ=255) ttCACCGGCCATCTGAGATG‑GCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAgcgc > 2:364640/1‑251 (MQ=255) aCCGGGCATCTGTGATG‑GCCGACAGGTTAACATAATCCCAGCCCTTTAGGGCCACGCGCTCAGCCCGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGCGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGCAATGATCCAGCGCGt > 2:26999/1‑250 (MQ=255) ccGGCCATCTGAGATG‑GCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAAt > 1:101456/1‑237 (MQ=255) ggCCATCTGAGATG‑GCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCAt > 2:143968/1‑106 (MQ=255) ggCCATCTGAGATG‑GCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCAt < 1:143968/106‑1 (MQ=255) aTG‑GCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTAtcatca > 2:354725/1‑250 (MQ=255) gACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGc > 1:66127/1‑251 (MQ=255) aGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGAGTTTATCATCAAGCGGGCGAt > 2:269864/1‑251 (MQ=255) ggggCCACGCGCCCAGCCATCGGAAGAAACGGAATCTAGACCCTTGTTAGC‑ACACCGTTCATCCCCCCCCGCCAGCTCGGCACTGAGCTGCGCCAAAAACTCGCCTATAAAATGCGCGGTGTGCGGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCGGGAAATGATCCAGCGCGTAGCGTTTATCAGCAAGCGGGCGATGCTGTGCGAACGGATCGTCACCAt < 2:17466/250‑1 (MQ=255) cgcgCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAAc > 1:31594/1‑249 (MQ=255) cgcgCCCATCCAGCGAATGAAAACGCATCTATCAGCTTGTGATCAACACC‑TTCATTCCCCCCCGCCAGTTCGGCACTGAGCTTCGGCATAAACTCGACGAAAAAATTCGCTTTGTGCTGCGCCATCTGCTTGCCTCGTTCGGTTTGCATGGTTTGCGGTAGCTTCGGCATTTTAGTCTGGAAATGAGCCTGCGCGTAGCGTGTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAg < 1:433011/251‑1 (MQ=255) gcgcTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCAGACGATGCTGTGc > 1:12701/1‑226 (MQ=255) agccagcGGATGAAAACGCATCTATCACCTTGTGATCAACCCC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTAGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCAcc > 2:370608/1‑250 (MQ=255) ccagcGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGcc < 1:388878/251‑1 (MQ=255) gcGGATGAAAACGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCa > 1:41377/1‑250 (MQ=255) cGCATCTATCCCCTTGTGATCAACACC‑TTCATTCCCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGt < 2:226658/153‑1 (MQ=255) cGCATCTATCACCTTGTGATCAACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGt > 1:226658/1‑153 (MQ=255) ccTTGTGATCAACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAAc > 1:36010/1‑215 (MQ=255) gATCAACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTCTCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAACGGCAAACACGCGCGcc > 1:273794/1‑251 (MQ=255) | GATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATG‑GCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACC‑TTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAACGGCAAACACGCGCGCC > NC_000913/2032188‑2032669 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |