Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,731,7240GA33.3% 50.3 / 11.6 36L198L (TTG→TTA) xylFxylose ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (17/7);  new base A (0/12);  total (17/19)
Fisher's exact test for biased strand distribution p-value = 4.52e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATTAACGATGCGGATATCGATTTTTATATTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCC‑GCGCCGGACAAATGAAAG‑TGTTAAAACC‑TTACGTTG‑ATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAA‑CGCATTGAAAATT‑ATGGAAAAC‑GCGCTAA‑CC‑GCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTT  >  NC_000913/3731476‑3731972
                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                            
aTTAACGATGCGGATAACGATTTTTATAGTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCC‑GCGCCGGACAAATGAAAG‑TGTTAAAACC‑TTACGTTG‑ATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAA‑CGCATTGaa                                                                                                                                                                                                                                                            <  2:114864/251‑1 (MQ=255)
  tAACGATGCGGATATCGATTTTTATATTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCC‑GCGCCGGACAAATGAAAG‑TGTTAAAACC‑TTACGTTG‑ATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAA‑CGCATTGaaa                                                                                                                                                                                                                                                           <  1:67530/250‑1 (MQ=255)
            gATATCGATTTTTATATTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCC‑GCGCCGGACAAATGAAAG‑TGTTAAAACC‑TTACGTTG‑ATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAA‑CGCATTGAAAATT‑ATGGaaa                                                                                                                                                                                                                                                >  2:91952/1‑250 (MQ=255)
                       ttATATTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCC‑GCGCCGGACAAATGAAAG‑TGTTAAAACC‑TTACGTTG‑ATTCCGGAAAAATTAAAGGCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAA‑CGCATTGAAAATT‑ATGGAAAAC‑GCGCTAA‑cc                                                                                                                                                                                                                                   <  1:91952/250‑1 (MQ=255)
                               ctttcGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCC‑GCGCCGGACAAATGAAAG‑TGTTAAAACC‑TTACGTTG‑ATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAA‑CGCATTGAAAATT‑ATGGAAAAC‑GCGCTAA‑CC‑GCCaataat                                                                                                                                                                                                                         >  2:168365/1‑251 (MQ=255)
                                  tcGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGAAGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCC‑GCGCCGGACAAATGAAAG‑TGTTAAAACC‑TTACGTTG‑ATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAA‑CGCATTGAAAATT‑ATGGAAAAC‑GCGCTAA‑CC‑GCCAATAATAAc                                                                                                                                                                                                                      >  1:77348/1‑251 (MQ=255)
                                                             aGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCC‑GCGCCGGACAAATGAAAG‑TGTTAAAACC‑TTACGTTG‑ATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAA‑CGCATTGAAAATT‑ATGGAAAAC‑GCGCTAA‑CC‑GCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAAc                                                                                                                                                                                           >  2:343228/1‑251 (MQ=255)
                                                                     gCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCC‑GCGCCGGACAAATGAAAG‑TGTTAAAACC‑TTACGTTG‑ATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGTTGGCTGGTTACCGGAAAA‑CGCATTGAAAATT‑ATGGAAAAC‑GCGCTAA‑CC‑GCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCAc                                                                                                                                                                                   >  2:105799/1‑251 (MQ=255)
                                                                         tGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCC‑GCGCCGGACAAATGAAAG‑TGTTAAAACC‑TTACGTTG‑ATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAA‑CGCATTGAAAATT‑ATGGAAAAC‑GCGCTAA‑CC‑GCCAATAATAACAAAATTGATGCTGt                                                                                                                                                                                                        >  2:89567/1‑226 (MQ=255)
                                                                         gggTCGATATTTTTCCGAAAGGTAATTCCTCCCTGAGGGGCGGCTCGCCGGTAAAAAACAACGCCAAGTTGTTCC‑GCGCCGGAAAAATGAAAG‑TGTAAAAACC‑TTACGTTG‑ATTCGGAAAAAATAAAAGTCGTTGGTGACAAATGGTTTGATGGCTGGTTACCGGAAAC‑CGCATTAAAAATT‑ATGAAAAAC‑GCGCTAA‑CC‑GCCAATAATAACAAAATTGATGCTGt                                                                                                                                                                                                        <  1:89567/225‑1 (MQ=255)
                                                                               atatTGTTCCGCAAGGTAATTCCTTCCTGAGGGGCGGCCCGCCGTTAGATAACACCGCAAGGCTGTTCC‑GCCCCGGA‑AAAAGAAAGGTGTTAAACCC‑TTACGTTG‑ATTCGGGAAAAATAAAAGTCTTTGGTGACCAATGGGTTGAGGGCTGTTTACCGAAAAA‑CTCTTTAAAAATT‑ATGGAAAAC‑GCGCTGA‑CC‑GCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTggg                                                                                                                                                                         <  1:60680/251‑1 (MQ=255)
                                                                                 atTGTCCCGCAGGGTAATTACTCCCGGAGGGGCGGCTCGCCGGAAAAAAACAACGCCAAG‑TGTTCCCGCCCAGAAAAAATGAAAG‑TGTTAAACCC‑TT‑CCTTGTATCCCGAAAAAATAAAAGTCGTTGGGCACCAATGGGTTGATGCTTGGTTACGGAAAAA‑CGCATTAAAATTT‑ATGAAAACC‑GCGCTAA‑CC‑GCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTgggg                                                                                                                                                                        <  2:347296/250‑1 (MQ=255)
                                                                                       ccGCAAGGTAATTACTCCCTGAGGGGCGGCCCGCGGTTAAAAAAAACCGCAAAGCTTTCCC‑GCGCCGAACAAATGAAAG‑TGTAAAACCC‑TTCGGTTG‑ATTCCGGAAAAATAAAAGTCGTTGGTGACAAATGGGTTGATGGCTGGTTACCGAAAAC‑CGCATTGAAAATT‑‑TGGAAAAA‑CCCCTAAACC‑GCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTTGGGCAATTc                                                                                                                                                                  <  1:343228/250‑1 (MQ=255)
                                                                                              gaaattgctccttgaggggCGGCTCGCCGGTAGAGAACAACGCCAAGCTGTTCG‑GCGCCGGACAAATGAAGG‑TGTAAAACCC‑TTACGTTG‑AGTCCGGAAAATTAAAAGTCGTTGGTGACTAATGGTTTGAGGGGGGTTTACGGGAAAA‑GTCATTAAAATTT‑ATAAAAAGG‑GCGCTAA‑CC‑GCCAATAATAACAAAATTGATGCTGTAGTGGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTa                                                                                                                                                          <  1:423658/235‑1 (MQ=255)
                                                                                               aaattattcccaggtGGGCGGCCCGCGGGTAGAAAAAAACCCCAGGCTGTTCC‑GCGCCGGAAAAATGAAAG‑TTTTAAAACC‑TTACGTTG‑ATTCCGAAAAAATTAAAGTCGTTGGGGACAAAGGGGTTGATGGCTGGTTACCGAAAAA‑CGCATTAAAAATT‑ATGAAAAAC‑GCGCTAA‑CC‑GCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTaa                                                                                                                                                         <  1:330585/237‑1 (MQ=255)
                                                                                                aaTTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCC‑CCGCCGGACCAATGAACG‑TGTTAAAACC‑TTACGCTG‑ATTCCGGAAAACTTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAA‑CGCATTGAAAATT‑ATGGAAAAC‑GCGCTAA‑CC‑GCCAATAATAACAAAATTGATGCTGTATTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTa                                                                                                                                                          >  1:243437/1‑249 (MQ=255)
                                                                                                        ccTGATGGCGGGCGCGCCGGAAGAAAACAACGCCAAGCTGTCCC‑GCGCCG‑AAAAAAGAAAA‑GGTTAAAACCCTTACGTTG‑ATTCCGAAAAAATAAAGGGCGTGGGTGACCAAGGGGTTGAGGGCGGGTTACCGGAAAA‑CGAATTAAAAATT‑AGGAAAAAC‑GGGCTAA‑CC‑GCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAg                                                                                                                                                <  1:212268/251‑1 (MQ=255)
                                                                                                                  ggcTCGCCGGTAGATAACAACGCCAAGCTGTTCC‑GCGCCGGACAAATGAAAG‑TGTTAAAACC‑TTACGTTG‑ATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAA‑CGCATTGAAAATT‑ATGGAAAAC‑GCGCTAA‑CC‑GCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAgg                                                                                                                                      >  1:198637/1‑251 (MQ=255)
                                                                                                                          ggTAGATAACAACGCCAAGCTGTTCC‑GCGCCGGACAAATGAAAG‑TGTTAAAACC‑TTACGTTG‑ATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAA‑CGCATTGAAAATT‑ATGGAAAAC‑GCGCTAA‑CC‑GCCAATAATAACAAAATTGAt                                                                                                                                                                                                             >  2:368269/1‑172 (MQ=255)
                                                                                                                          ggTAGAAAACAACGCCAAGCTGTTCC‑GCGCCGGAAAAATGAAAG‑TGTTAAAACC‑TTACGTTG‑ATCCCGAAAAAATAAAAGTCGTGGGTGACCAAGGGGTTGATGGCTGGTTCCCGAAAAA‑CGCATTAAAAATT‑ATGAAAACC‑GCGCTAA‑CC‑GCCAATAATAACAAAATTGAt                                                                                                                                                                                                             <  1:368269/172‑1 (MQ=255)
                                                                                                                                        ccAAGCTGTTCC‑GCGCCGGACAAATGAAAG‑TGTTAAAACC‑TTACGTTG‑ATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAA‑CGCATTGAAAATT‑ATGGAAAAC‑GCGCTAA‑CC‑GCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGcc                                                                                                                  >  2:373596/1‑249 (MQ=255)
                                                                                                                                                         cGGACAAATGAAAG‑TGTTAAAACC‑TTACGTTG‑ATTCCGAAAAAATTAAAGTCGTTGGTGCCAAAGGGGTTGATGGCTGGTTACCGAAAAA‑CGCATTGAAAATT‑A‑GGAAAACCGCGCTAA‑CC‑GCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAg                                                                                                <  1:226674/251‑1 (MQ=255)
                                                                                                                                                            aCAAATGAAAG‑TGTTAAAACC‑TTACGTTG‑ATTCCGGAAAAATTAACGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAA‑CGCATTGAAAATT‑ATGGAAAAC‑GCGCTAA‑CC‑GCCAATACTAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGCAAGAAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGt                                                                                              >  1:280877/1‑250 (MQ=255)
                                                                                                                                                                         gtTAAACCC‑TTACGTTG‑ATTCCGAAAAAATTAAAGTCGTTGGTGACCAATGGGTTGAGGGCTGGTTACCGAAAAA‑CGCATTAAAAATT‑ATG‑AAAAC‑CCCCCAA‑CCCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTAtt                                                                                  <  1:124918/250‑1 (MQ=255)
                                                                                                                                                                                cc‑TTACGTTG‑ATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAA‑CGCATTGAAAATT‑ATGGAAAAC‑GCGCTAA‑CC‑GCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCgg                                                                          >  1:8969/1‑251 (MQ=255)
                                                                                                                                                                                     taggtggtttCCGAAAAAATAAAAGTCGTGGGTGACCAAGGGTTTGATGGCTGGTTACCGAAAAA‑CGCATTAAAAATT‑AGGGAAACC‑GCGCTAA‑CC‑GCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTa                                                                        <  1:373596/242‑1 (MQ=255)
                                                                                                                                                                                                 ggAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAA‑CGCATTGAAAATT‑ATGGAAAAC‑GCGCTAA‑CC‑GCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGGATTAAACGTATTGCTGCCGGTACGCAAACTATGAc                                                           >  1:110133/1‑251 (MQ=255)
                                                                                                                                                                                                 aaaaaaataaaggtgtttGGAGACAAATGGGTTGATGGCGGGGT‑CCGGAAAAACG‑AATGAAAATTTATGAAAACC‑GCGCTAG‑CC‑GCCAATAATAACAAAATTGATGCTGTAGTTGCCGCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAATGGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCGGGCCAGGATGCGGATCTCGCAGGGATTAAACGTATTGCTGCCGGTACGCAAACTATGAc                                                           <  2:231603/235‑1 (MQ=255)
                                                                                                                                                                                                            aGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAA‑CGCATTGAAAATT‑ATGGAAAAC‑GCGCTAA‑CC‑GCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAAc                                                >  2:94486/1‑251 (MQ=255)
                                                                                                                                                                                                                           aTGGGTTGATGGCTGGTTACCGGAAAA‑CGCATTGAAAATT‑ATGGAAAAC‑GCGCTAA‑CC‑GCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTgg                                 >  1:92658/1‑251 (MQ=255)
                                                                                                                                                                                                                                  gATGGCTGGTTACCGGAAAA‑CGCATTGAAAATT‑ATGGAAAAC‑GCGCTAA‑CC‑GCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCACTTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCACTCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATAc                          >  1:457916/1‑251 (MQ=255)
                                                                                                                                                                                                                                   aTGGCTGGTTACCGAAAAC‑CGAATTAAAATTT‑ATGGAAACC‑GCGCTAA‑CC‑GCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACt                         <  1:453397/251‑1 (MQ=255)
                                                                                                                                                                                                                                     gggggtttACCGGAAAG‑CGTATTAAAAATT‑GTGGAAACC‑GCGCTAA‑CC‑GCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTg                        <  1:257237/244‑1 (MQ=255)
                                                                                                                                                                                                                                      gctggtgacggaaaaa‑CGAATTAAAAATT‑ATGAAAACC‑GCCCAAA‑CC‑GCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGcc                      <  2:148405/239‑1 (MQ=255)
                                                                                                                                                                                                                                               cGGAAAA‑CGCATTGAAAATT‑ATGGAAAAC‑GCGCTAA‑CC‑GCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAAtt             >  2:217127/1‑251 (MQ=255)
                                                                                                                                                                                                                                                            tGAAAATT‑ATGAAAAGC‑GCGCAAA‑CC‑GCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGtt  <  1:168365/250‑1 (MQ=255)
                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                            
ATTAACGATGCGGATATCGATTTTTATATTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCC‑GCGCCGGACAAATGAAAG‑TGTTAAAACC‑TTACGTTG‑ATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAA‑CGCATTGAAAATT‑ATGGAAAAC‑GCGCTAA‑CC‑GCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTT  >  NC_000913/3731476‑3731972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑