Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,135,7750CT40.0% 32.4 / 13.9 30K199K (AAG→AAA) pitBmetal phosphate:H(+) symporter PitB
Reads supporting (aligned to +/- strand):  ref base C (3/15);  new base T (12/0);  total (15/15)
Fisher's exact test for biased strand distribution p-value = 1.05e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGG‑AATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGC‑AGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTAAACCGATGCCGATAATCGCACCAATCAAGGTGTGCGAACTGGAGGC  >  NC_000913/3135528‑3136020
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       
gCGGCAGTTCAGGATGCTGTTGCGGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCttt                                                                                                                                                                                                                                                      <  2:26576/251‑1 (MQ=255)
gCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCt                                                                                                                                                                                                                                                        <  1:109345/249‑1 (MQ=255)
 cGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGACGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCCATACGCGTCCAGAATGGCGGTTTACGGTTGTCTtttttttttt                                                                                                                                                                                                                                                     >  2:131510/1‑251 (MQ=255)
         cAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGGTTATGTTTGTCTTTTTTTTTTTTgtgctct                                                                                                                                                                                                                                             >  2:34864/1‑245 (MQ=255)
           ggATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGCTTGTCTTTTTTTTTTTTGTGttcttc                                                                                                                                                                                                                                           >  2:110181/1‑251 (MQ=255)
            gATGCTGTTGCAGGTAGTGTTCGAAGGTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGGGCGAAGACGCCACGCCCGCAGCGGAAACAATCAGCGCAATTCGCGTCCAGAATGGCGGTTTTCGTTTGCCTTTTTTCTTTTTGCGATCTTcc                                                                                                                                                                                                                                          <  1:36695/251‑1 (MQ=255)
                                        ggTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCttt                                                                                                                                                                                                               <  1:349656/250‑1 (MQ=255)
                                           aaCGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTc                                                                                                                                                                                                            <  1:190167/250‑1 (MQ=255)
                                                          acgggtaATTTCATAGCCGGACGCATTCATATTGACGCCGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGCCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTATATATGCCTTTTTTTTTTTTTCCATCTTTCGGGATTAGGGGAAAACAGTCACGCTTTTTCGTTCCGccacactagc                                                                                                                                                                                            >  1:160263/1‑242 (MQ=255)
                                                          acgggtaATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTTCGTTTGCCTTTTTTTTTTTTGTGATCTTCCGGGATGCGGGGCATACGGTCCCGCTTTTTTGTCCCGcccccatagg                                                                                                                                                                                            >  1:282194/1‑242 (MQ=255)
                                                                aaTTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTTTCTTTTTTTTTTTTTCGGTCTTCCGGAAGGGGGTGAATACGGTCACGCTTTTTTCTCCCGCCCCACTAGCGTCgcc                                                                                                                                                                                      >  1:238190/1‑250 (MQ=255)
                                                                         gCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTTTTTTTTCGATCTTCCCGAATGCGGGGGAAACGGTCACGCTTTTTTGTCCCCCCCCAGTAGCGTCgccgccgggat                                                                                                                                                                              >  1:318115/1‑241 (MQ=255)
                                                                                              gacgaAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGG‑AATATCAGGCCTCCCGCAATGACCa                                                                                                                                                       <  1:303386/251‑1 (MQ=255)
                                                                                              gacgaAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATCGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGG‑AATATCAGGCCGCCCGCAATGACCa                                                                                                                                                       <  2:17511/251‑1 (MQ=255)
                                                                                                         cagGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTTTTTTTGCGCTCTTCCGGAATGCGGTGAATAAGGTCACGCTTTTTTGTCCCGCTCCCGTAGCGTCGCAGCAGG‑GAAATCAGGGCTCCCGCCATGACCAGGcgcacg                                                                                                                                               >  2:267440/1‑243 (MQ=255)
                                                                                                              gCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCGTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGG‑AATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGa                                                                                                                                       <  1:325131/251‑1 (MQ=255)
                                                                                                                aaTCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGGCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCg                                                                                                                                                                                                      <  2:330880/187‑1 (MQ=255)
                                                                                                                            tACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTAAGTTTTCCTTTTTTTTTTTTTCGATCTTCCGGGATGGGGGGGTTACGGTCACGCTTTTTTGGCCCCCCCCACTAGCGTCGCCACAGGGAAAATC‑GGCCCCCCGCAATGACCCGGCcgccgcaacggggaaacaacatcgagg                                                                                                                         >  2:22430/1‑226 (MQ=255)
                                                                                                                                 gCATTACCAGGCCGATCCCTTTTTGTCCGGCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTGGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTGTCGTCCCGCTCCAGTAGCGTCGCAGCAGG‑AATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGaaa                                                                                                                    <  2:318115/251‑1 (MQ=255)
                                                                                                                                  cATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTTCGTTTGCCTTTTTTTTTTTTTCGATCTTCCGGAATGCGGGGAATAAGGTCCCGCTTTTTCGTCCCGCCCCAGTAGCGTCGCCGCAGG‑GATATCTGGGCTCCCGCCAAGACCAGGCCGACGGTAGGGGGAACAAACAGCgaggggcaa                                                                                                                    >  2:249518/1‑245 (MQ=255)
                                                                                                                                            ccGATCCCTTTTTGTCCGTCGTTAGCGCAGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGGGCATACGGTCACGCTTTTTCGTCCCACCCCAGTAGCGTCGCAGCAGG‑GATATTAGGCCTCCCGCAATGACCCGGCCGACGATAGGGGGAACAATCAGCGAGGAGAAAATTTTGGTCAc                                                                                                         >  1:124947/1‑251 (MQ=255)
                                                                                                                                                              tcgtTCGCGCCGTGCGAAAACGCCACGCCCGCCGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTCCGTTTGCCTTTTTTTTTTTTGCGATCTTCCCGACTGCGGGGAATACGGTCCCGCTTTTTCGTCCCGCTCCAGTAGCGCCGCAGCAGG‑GATATACGGCCCCCCGCCATGACCCGGCCCACGCTAGGGGGAACACTCAGCGAGGAGAAAAATTTGGTCCCTTCACGCCAGGTT‑AACGc                                                                                      >  2:217888/1‑251 (MQ=255)
                                                                                                                                                                       ccGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGG‑AATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGC‑AGGTTTAACGCATCCATCAc                                                                             <  1:200137/251‑1 (MQ=255)
                                                                                                                                                                        cGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTTTTTTTGCGATCTTCCGGAATGCGGGGAATACCGTCACGCTTTTTCTTCCCGCTCCAGTAACGTCGCAGCCGG‑GATATTCGGCCTCCCGCAAAGACCCGGCCGGCGCAAGGGGGAACAAACAGCGGGGAGACAATTTTGGTCACTTCCCGC‑CGGGTTAACGCATCCCTCAcc                                                                            >  2:81176/1‑251 (MQ=255)
                                                                                                                                                                        cGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGG‑AATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGC‑AGGTTTAACGCATCCATCAcc                                                                            <  1:267440/251‑1 (MQ=255)
                                                                                                                                                                                 acgccacgccCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGATTACGGTTTCCTTTTTTCTTTTTGAGATCTTCCGGAATGCGGGGGAAACGGTCACGCTTTTTCGTCCCGCTCCCGTAGCGTCGCAGCCGG‑AATATCAAGCCTCCCGCAATGACCCGGCCGACGATAGGGGGAAAAAAAAACGAGGAGAAAATTTTGGTCACTTCACCC‑CGGTTTTAAGCATCCATCACCCATGAGCCg                                                                   >  1:85774/1‑251 (MQ=255)
                                                                                                                                                                                     cacgccCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGG‑AATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGC‑AGGTTTAACGCATCCATCACCGATGAGCCGGTTa                                                               <  1:110181/251‑1 (MQ=255)
                                                                                                                                                                                                   aaaCAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGG‑AATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGC‑AGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGtt                                                 <  2:289904/251‑1 (MQ=255)
                                                                                                                                                                                                             cgcAATACGCGTCCAGAATGGCGGTTTACGGTTGCCTTTTTTTTTTTTGCGATCTTCCGGAATGCGGTGAATACGGGCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGG‑AATATCAGGCCTCCCGCAATGACCAGGCCGGCGATAGGGGGAACAATCAGAGAGGGGAAAATTTTGGGCACCTCACGC‑AGGGTTAAAGCATCCATCACCGATGAGCCGGGTTACAGCGCGTTGGTTAAACCGATGc                                       >  1:62103/1‑251 (MQ=255)
                                                                                                                                                                                                                                          gTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGG‑AATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGC‑AGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTAAACCGATGCCGATAATCGCACCAATCAAGGTGTGCGa           >  1:246239/1‑250 (MQ=255)
                                                                                                                                                                                                                                                   ttttCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGG‑AATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGC‑AGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTAAACCGATGCCGATAATCGCACCAATCAAGGTGTGCGAACTGGAGGc  <  1:81176/250‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       
GCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGG‑AATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGC‑AGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTAAACCGATGCCGATAATCGCACCAATCAAGGTGTGCGAACTGGAGGC  >  NC_000913/3135528‑3136020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑