Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,134,744	C→A	P303P (CCG→CCT)	wzc ←	protein‑tyrosine kinase Wzc

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,134,744	0	C	A	100.0%	226.5 / NA	66	P303P (CCG→CCT)	wzc	protein‑tyrosine kinase Wzc
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (38/28);  total (38/28)

GACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGTCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCC  >  NC_000913/2134498‑2134990                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       gACCTGCTGACCAGACTCGACATCGCGGGTCAGAAGGACAATATCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGcc                                                                                                                                                                                                                                                    >  2:385001/1‑250 (MQ=255)      gctgACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGCTTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATc                                                                                                                                                                                                                                                >  2:455235/1‑250 (MQ=255)      gctgACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTGTGCTTCCAGAGGCAGAt                                                                                                                                                                                                                                                 <  1:159594/249‑1 (MQ=255)                    aCATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCCGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATc                                                                                                                                                                                                                                 >  1:511117/1‑251 (MQ=255)                    aCATCGCGGGACAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTCACGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCACCAGCATCTTTATc                                                                                                                                                                                                                                 >  2:290476/1‑251 (MQ=255)                     cATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGCATCTTTATcc                                                                                                                                                                                                                                >  2:350546/1‑251 (MQ=255)                     cATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATcc                                                                                                                                                                                                                                <  1:293381/251‑1 (MQ=255)                        cgcgGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGa                                                                                                                                                                                                                             <  2:174362/251‑1 (MQ=255)                                 gACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGct                                                                                                                                                                                                                    >  2:535619/1‑250 (MQ=255)                                               tgctgGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTc                                                                                                                                                                                                      >  1:382003/1‑251 (MQ=255)                                                  ggggTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGc                                                                                                                                                                                                   <  2:382003/250‑1 (MQ=255)                                                   gggTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGCTGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGCTCACCAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGcc                                                                                                                                                                                                  >  2:217508/1‑250 (MQ=255)                                                    ggTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCa                                                                                                                                                                                                  >  2:160659/1‑250 (MQ=255)                                                       tttCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACAt                                                                                                                                                                                              >  2:413192/1‑251 (MQ=255)                                                        ttCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACAt                                                                                                                                                                                              <  1:455235/250‑1 (MQ=255)                                                         tCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATcc                                                                                                                                                                                            >  2:159956/1‑251 (MQ=255)                                                              aTCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCg                                                                                                                                                                                       >  2:108010/1‑251 (MQ=255)                                                                  ccGTGACGCGCCCGTAAAGTGTGGCTTTTTCGCCTTCCAGCGCCTGACTGTTCCCCACCAGCGTGCGGTCCGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTGCGTTCAACTGCGCATCTATGTTCACCATCGAATCGAGCACCGCTTTTTCTTCCAGAGGCAGATCAACAGAATCGTTATCCTGACGGAAGGCATTCAGTTTGGTGTCGGCGACATCCAAGCGGCTa                                                                                                                                                                                   <  2:521276/251‑1 (MQ=255)                                                                         gcgACCTTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCCGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTGGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAATCGGCTACGTACtt                                                                                                                                                                            <  2:110889/251‑1 (MQ=255)                                                                               gTTAAGTTTGGCTTTTTCGTGTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGACCTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGAATCGATGTTCCCCATCGACTCGAGCACCGCTTTCGCTTCCAGAGGCAGATCAACAGACTCTTTATCCTGACGGAAGGGATTCTGTTTGTTTTCGGCAACATCCAAGCGGCtacttactccaggt                                                                                                                                                                       >  2:335705/1‑244 (MQ=255)                                                                                       tGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTgc                                                                                                                                                              >  1:426321/1‑251 (MQ=255)                                                                                        ggCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGCTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTgcg                                                                                                                                                             >  1:299933/1‑251 (MQ=255)                                                                                             tttCGTCTTCCAGCGCCTGACGTTTCTACAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGACTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAgg                                                                                                                                                        >  2:32953/1‑251 (MQ=255)                                                                                                    ttCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGCGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCg                                                                                                                                                  >  1:94986/1‑250 (MQ=255)                                                                                                    ttCCAGCGCCTGAAGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCg                                                                                                                                                  >  1:449254/1‑250 (MQ=255)                                                                                                     tCCAGCGCCTGACGTTTCGCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAAGCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGc                                                                                                                                                   <  1:32953/248‑1 (MQ=255)                                                                                                        aGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCt                                                                                                                                             <  1:347419/251‑1 (MQ=255)                                                                                                                         cagcagCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTGCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTccgccg                                                                                                                            <  1:175012/251‑1 (MQ=255)                                                                                                                             ggcGTGCGGTGCGCCGGGTGATCTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTGTGTGTTCGGCAACATCCAAGCGGCGACGTACTGCCGGTAACTGTGGCGCGAGTAAGGCGATGCTTTTCGACGCTTCCGCCGAttt                                                                                                                        <  2:504829/250‑1 (MQ=255)                                                                                                                             agcGTGCTGTTCGCCGGGTGAA‑TTTGGTTTTACAGCTGGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCTCCGCTTTTGCTTCCAGAGGCAGATCTACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTGTTCAACGCTTCCGCCGAttt                                                                                                                        <  2:51314/251‑1 (MQ=255)                                                                                                                               ggTGCGGTACGCCGGTTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTgc                                                                                                                      <  2:72101/250‑1 (MQ=255)                                                                                                                               cGTGCGGTACGCGGGGTGAACTTTGGTGT‑ACAGCTTGGACATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTGCTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTgc                                                                                                                      <  2:426321/251‑1 (MQ=255)                                                                                                                                  gCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTGCGACGCTTCCGCCGATTTgcgc                                                                                                                    <  1:462406/250‑1 (MQ=255)                                                                                                                                    ggTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTc                                                                                                                  >  1:337822/1‑250 (MQ=255)                                                                                                                                          ccgtgTGAACTGTGGTGG‑ACAGGTTGGAGGTTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCGGTTGCTTCCAGAGGCAGATCAACTGAATCTTTATCCTTACGGAAGGCAGTCAGTTTGTTTGCGGCAACATCCAAGCGGCGTCGTGCTTCCGGTAACTGTTGCGCGCGTAAGTCGATGCTTTGCTTCGCTTCCGCCTATTTGCGCTCAATAttt                                                                                                           <  2:536224/247‑1 (MQ=255)                                                                                                                                          ccGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCACTTCGTTCAAATGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGAAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATAttt                                                                                                           >  1:33753/1‑251 (MQ=255)                                                                                                                                            gggTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCGTCCGCCGATTTGCGCTCAATATTTTg                                                                                                         <  1:159956/251‑1 (MQ=255)                                                                                                                                                 aaCTTTGGTGG‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTGCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCt                                                                                                    <  2:514777/251‑1 (MQ=255)                                                                                                                                                  aCTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTg                                                                                                   <  1:466945/251‑1 (MQ=255)                                                                                                                                                                gCTTGGAGATTTCCGCCTCTTTAAGGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGCAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTAGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCg                                                                                      >  2:74311/1‑251 (MQ=255)                                                                                                                                                                gCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCg                                                                                      <  1:350546/251‑1 (MQ=255)                                                                                                                                                                  gtGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGCTCAACTGCGAATAGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGAt                                                                                    >  2:350146/2‑251 (MQ=255)                                                                                                                                                                         tttCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAgg                                                                                                                                               <  1:345401/185‑1 (MQ=255)                                                                                                                                                                         tttCCGCCTCTTTAAAGGTCAGTTCGTTAAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAgg                                                                                                                                               >  2:345401/1‑185 (MQ=255)                                                                                                                                                                          ttCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGACGTTCACCATCGAATCGAGCACCGCTTTCGCTTCCAGAGGCAGATCACCAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGCAGGCGCGGCTTTTCGACGCTTCCGCCGCTTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTa                                                                             >  1:533653/1‑250 (MQ=255)                                                                                                                                                                             cGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGa                                                                          >  1:505441/1‑250 (MQ=255)                                                                                                                                                                              gCCTCTTTAAAGGGCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCGCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAAt                                                                        <  2:315864/251‑1 (MQ=255)                                                                                                                                                                                   tttAAAGGTCAGCTCGTTCAACTGCGCATCGATGTTCCCCATCGAATCGCGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGCCGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTcgc                                                                   >  2:278792/1‑251 (MQ=255)                                                                                                                                                                                    ttAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTcgc                                                                   <  1:425433/250‑1 (MQ=255)                                                                                                                                                                                          gTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTg                                                            <  1:464443/251‑1 (MQ=255)                                                                                                                                                                                             aGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGCGCACAGCTTTTGCTTCCAGAGGCAGATCAACAGAATATTTATCCTGACGGCAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGCAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTc                                                         >  1:58747/1‑251 (MQ=255)                                                                                                                                                                                                tcgttcAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCAc                                                       <  1:385001/250‑1 (MQ=255)                                                                                                                                                                                                  gttcAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGCTCTGTTCACGAt                                                    >  2:196637/1‑251 (MQ=255)                                                                                                                                                                                                    tcAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTg                                                                                <  2:94071/221‑1 (MQ=255)                                                                                                                                                                                                    tcAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTg                                                                                >  1:94071/1‑221 (MQ=255)                                                                                                                                                                                                    tcAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCt                                                  >  1:158299/1‑251 (MQ=255)                                                                                                                                                                                                            gcATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGt                                          >  1:274647/1‑251 (MQ=255)                                                                                                                                                                                                            gcATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGt                                          >  2:27958/1‑251 (MQ=255)                                                                                                                                                                                                                    gTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGTc                                   >  2:80957/1‑250 (MQ=255)                                                                                                                                                                                                                        aCCATCGAATCGAGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGTCAGGCt                              >  1:355526/1‑251 (MQ=255)                                                                                                                                                                                                                                   gaGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGTCAGGCTCAAAACGcc                     >  1:407983/1‑249 (MQ=255)                                                                                                                                                                                                                                    aGCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGACGGCATTCCGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGCCGCCTCCGCCGCTTTGCGCTCAATATTTTGTTCCTGATTGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTCTAAGTCAGGCTCAAAAAGCCAGc                  >  2:51931/1‑251 (MQ=255)                                                                                                                                                                                                                                     gCACCGCTTTTGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTGCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTGAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGTCAGGCTCAAAACGCCAGCg                 <  1:278792/251‑1 (MQ=255)                                                                                                                                                                                                                                              ttGCTTCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGTCAGGCTCAAAACGCCAGCGTCTTTGCCg        >  2:151332/1‑251 (MQ=255)                                                                                                                                                                                                                                                  ttCCAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATCAGTCAGGCTCAAACCGCCAGCGTCTTTGCCGTtct    >  2:450090/1‑251 (MQ=255)                                                                                                                                                                                                                                                    ccAGAGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACTTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGTCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTcc  <  1:290476/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       GACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGT‑ACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGTCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCC  >  NC_000913/2134498‑2134990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑