Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	83.8 / NA	25	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/11);  total (14/11)

GTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATGC  >  NC_000913/1652100‑1652575                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     gTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGGCATTCATCTTATTAAGCCAGCAATCGACCCCCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGGATGGcagaa                                                                                                                                                                                                                                    >  1:62565/1‑250 (MQ=255)   ttATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGCTGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCCAAAACACAGGCGGGAACCCATCGGGGCATTTATCTTATTAAGCCAGCAATCGACGCCCTCCGGGGTCAAATGACATTAACGAGACTGAGCAAAGAGGATATCATTGATGTTCACCTCagggagg                                                                                                                                                                                                                                             >  1:364197/1‑238 (MQ=255)   ttATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGt                                                                                                                                                                                                                                             <  2:364197/239‑1 (MQ=255)                         gAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACAGTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGGATGGCAGAACAGAAAAACAAAAATGCACCTTTg                                                                                                                                                                                                            <  2:489417/250‑1 (MQ=255)                                    aCTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTGTTCAACCTg                                                                                                                                                                                                <  2:31987/251‑1 (MQ=255)                                                              aaaaTGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCGCCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAt                                                                                                                                                                      <  1:268122/251‑1 (MQ=255)                                                                   ggAACAATGATGATCAGGAGAAATTTATCAAAAGACCGTTTCACGGTACCAAAAACACATGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCATCCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGa                                                                                                                                                                 <  2:5545/251‑1 (MQ=255)                                                                   ggAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACAAAAAACACAGGCGGGAAACAAAAGGGGCCTTCACCTTATTTAGCCCACACTCGTCGCCATCCCGAGTCAGATGAACTTATCATGACTGAACAAATAGCCAATCTTTGGAGTTCACCCCCACGAGGAAGGCAGGACAGGAAAAAAAAAATGCACCTTTGTTTTTCAACCTGAAGACTCAGCgaggagcaaaaatatatgtg                                                                                                                                                                  >  2:293420/1‑233 (MQ=255)                                                                                 cAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCAtttt                                                                                                                                                          <  1:97350/244‑1 (MQ=255)                                                                                            tAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAg                                                                                                                                         <  1:397132/250‑1 (MQ=255)                                                                                            tAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAACGGGGCATTCATCTTATTAAGACAGCAATCGGCGCTTTCCGGAGTTAGATGAAATTTACGAGGCTGAGTAAAAAGGATATCATTTATGGTTACCTCAGAGAGTATGGCAGAAAAGAAAAAAAAAAAAGAACCTTTGTTTTTCAAACTGAAGGGTTAGCGAGAGTAAAAAATAATTGGGACCAATTTACCGtgtagctcaaaagg                                                                                                                                        >  2:131850/1‑238 (MQ=255)                                                                                                             aCGGTACCAAAAACACAGGCGGGAACCAATCGGGTCCTTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGc                                                                                                                       >  1:142560/1‑251 (MQ=255)                                                                                                                                         aTCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAGCCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCgg                                                                                                        >  1:211665/1‑238 (MQ=255)                                                                                                                                         aTCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAGCCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCgg                                                                                                        <  2:211665/238‑1 (MQ=255)                                                                                                                                             ggTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGCGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGCACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCCGCGAGAGTAAAAAATTATGGTAACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCCGCGATAAAACGTGCCGGACTCCGCCATCGAAAAt                                                                                       >  1:326786/1‑251 (MQ=255)                                                                                                                                                   tcatCTTATTACGCCAGCAATCACCGCTCCCCGGAGTCAGACGACATCAACGAGCATAAGCACCGCGCATATCATTGATGTTCACCTCAGAGAGTCTGGCAGAACAGAAAAACACAAATGCACCTTTGTTTTTCACCCTGAAGTGTCAGCGAGAGCAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGCAGCAGCGATAACACGTGCCGGCATCCGCCATCGAACATCATATc                                                                                 >  2:236974/1‑251 (MQ=255)                                                                                                                                                            taAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATACCAGTCgaga                                                                         <  2:104264/250‑1 (MQ=255)                                                                                                                                                             aaGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGAc                                                                        >  1:523256/1‑250 (MQ=255)                                                                                                                                                                   gCAATCGCCGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACACAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGACGAGACATACtt                                                                  >  2:221746/1‑250 (MQ=255)                                                                                                                                                                      aTCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGc                                                              <  1:207685/251‑1 (MQ=255)                                                                                                                                                                                                 ttAACGAGAATGAGCAAAGAGCATATCATCGATGTTCACCTCAGAGAGTCTGGCAGAACCGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGCAAAAAATTATGGTGACCATTTTACCGTTGACTCAATCAGGCAGATGTGGGACGCAGCGATAACAAGTGGCGGACTCCGCCATCGAAACTCATATAAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTggggcga                                    >  2:315247/1‑245 (MQ=255)                                                                                                                                                                                                   aaCGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAAcc                                 >  2:129482/1‑251 (MQ=255)                                                                                                                                                                                                                aaaGAGCATATCATTGATGTTCACCTCAGAGAGTATGCCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGc                    >  1:482250/1‑251 (MQ=255)                                                                                                                                                                                                                     gCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAAcc               <  2:62565/251‑1 (MQ=255)                                                                                                                                                                                                                                  gTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGACCCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCAGCCATCGACAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATgg  >  1:510428/1‑250 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     GTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATGC  >  NC_000913/1652100‑1652575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑