Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,337,394	A→G	S522G (AGC→GGC)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,337,394	0	A	G	100.0%	180.0 / NA	55	S522G (AGC→GGC)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/32);  total (23/32)

CCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATC‑TGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCCAC  >  NC_000913/1337150‑1337638                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       cccAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAACAAAGCCGTAACTATGGGCCTCAAGCGGCAACCCTGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTACCCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGAtt                                                                                                                                                                                                                                                  >  1:499071/1‑251 (MQ=255) cccAAGGGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTGAACCTTGTGGGATACGGGTGTACCACCGGGATTGGTAACTCTGGGCCTCTGCCCGATCCTAGCGAAACTGCAATCAAAAAAGGCGAtt                                                                                                                                                                                                                                                  <  2:343537/251‑1 (MQ=255)    aaGTGTGCTGAAGGCCGCACGCTTTCTGGCGTAAAAAGCCGTAACTCTGGGCCTCAAGCTGCAACCATGGGTCAAAGCGTCGCTGGCACCTGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAGCGGACACCGTATCGCGACGAACGGTGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTAGTAGTATCTCTGGGCCGCTGCCCTATCCTAGCGAAACGTCAATCAAAAAAGGCGATTTaa                                                                                                                                                                                                                                               <  2:112349/251‑1 (MQ=255)      gtgtGCTGATTGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGGCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTTGGGTTTAGCCTTGTGGTATACGGTTGTCCCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACTGCAATCATAAAAGGCTATTTAAcc                                                                                                                                                                                                                                             <  2:72867/251‑1 (MQ=255)                ggCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAGCTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCAGCTGTATTGGGAACTCTGGGCGGCTGCCCGATCCGATCGAAACGGCAATCAAAAAAGGCGAGTGAACCGGcggtgcgg                                                                                                                                                                                                                                   <  2:191178/251‑1 (MQ=255)                   cGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCggtgcggtgc                                                                                                                                                                                                                                >  1:141817/1‑251 (MQ=255)                     cAGGGTTGCGGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACGGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTg                                                                                                                                                                                                                              <  2:320331/251‑1 (MQ=255)                                   aaaaaaGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTaa                                                                                                                                                                                                                <  1:302672/251‑1 (MQ=255)                                     aaaaGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTa                                                                                                                                                                                                                 <  2:106947/248‑1 (MQ=255)                                     aaaaGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTa                                                                                                                                                                                                                 >  1:106947/1‑248 (MQ=255)                                                    gggCCTCAAGCGGCAACCAGGGGTCAAAGCGTCGCTGGCACCGGTTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAATTGGGGTTTAACCTTGTTGGATACGGTTGTACCACCTGTATGGGTAACTCTGGGCCGCTGCCCGTTCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTatc                                                                                                                                                                                               <  1:364979/250‑1 (MQ=255)                                                          ccaGCGGCAACCATGGGCCAAAGCGTCGCTGGCACCGGGGACGAAAGTCGTTTCTGATTATATGGCAAAAGCGAAACTGACTCCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATCAGGTTGTACCACCTGTATTGTTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTGAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTatccatcc                                                                                                                                                                                          <  2:169568/247‑1 (MQ=255)                                                                                                    gAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTCAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTg                                                                                                                                              >  2:164183/1‑251 (MQ=255)                                                                                                      aaGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCACTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGcc                                                                                                                                            >  1:17915/1‑251 (MQ=255)                                                                                                          cGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATg                                                                                                                                        <  1:492522/251‑1 (MQ=255)                                                                                                          cGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATg                                                                                                                                        <  1:323449/251‑1 (MQ=255)                                                                                                             ttCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTGAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATgcg                                                                                                                                      <  1:457894/250‑1 (MQ=255)                                                                                                                  aTTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAATCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGACGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGcc                                                                                                                                                                                                    >  2:203082/1‑183 (MQ=255)                                                                                                                  aTTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGGCGGTGCGGTGCTGGCCGGCAACCGTAAC‑TTTGAAGGcc                                                                                                                                                                                                    <  1:203082/183‑1 (MQ=255)                                                                                                                     aTCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCggg                                                                                                                              >  1:225444/1‑250 (MQ=255)                                                                                                                     aTCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCggg                                                                                                                              <  1:227275/250‑1 (MQ=255)                                                                                                                      tCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCAGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGa                                                                                                                             >  2:298586/1‑250 (MQ=255)                                                                                                                         ggCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAat                                                                                                                          >  1:178987/1‑250 (MQ=255)                                                                                                                         ggCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGGTGCCTATGCGCGGGCGGGa                                                                                                                             <  1:420534/247‑1 (MQ=255)                                                                                                                         ggCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACGGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGa                                                                                                                             <  2:246242/247‑1 (MQ=255)                                                                                                                          gCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAGCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAatat                                                                                                                        <  2:58767/251‑1 (MQ=255)                                                                                                                                     aCTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAAcc                                                                                                             >  1:212410/1‑251 (MQ=255)                                                                                                                                        gACACCGTAGCTCGACGAACGGGGGTTTAACCGTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGTCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCGCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCt                                                                                                            <  2:284004/249‑1 (MQ=255)                                                                                                                                         acacCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGc                                                                                                         <  1:189450/251‑1 (MQ=255)                                                                                                                                          cacCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCt                                                                                                        <  1:17076/251‑1 (MQ=255)                                                                                                                                                    cgacgaACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGccc                                                                                               >  2:429805/1‑249 (MQ=255)                                                                                                                                                     gacgaACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCCTCTGAGCCTAt                                                                                             >  2:74188/1‑251 (MQ=255)                                                                                                                                                         aaCTGGGGTTTTACCTAGTCGGACACGGTTCTACCACCTGTATTGGTAACTCTGGGCCGCTGCCAGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTACGGCAACCGTCAC‑TTTGAAGGCCGTATCCCTCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGATGGTGGTTGCCTATGCGCTGGCGGGCAATATGAATATCCACCTggacttcgagccctccggg                                                                                         >  2:354553/1‑234 (MQ=255)                                                                                                                                                          aCTGGGGTTTAACCTTGTGGGAGACGGTTGTACCACCTGTATGGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCTGTATCCATCCGCTGGTTAAAACTAACTGTCTGGCCTCGCCGCCGCTTGTGGGTGCCTACGGGCTGGCTGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGcc                                                                                        <  1:26747/251‑1 (MQ=255)                                                                                                                                                                gTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTTGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCCACCTGGCTTCTGAGCCTATCGGCCATGATc                                                                                  >  1:26703/1‑251 (MQ=255)                                                                                                                                                                gTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATc                                                                                  <  1:263774/251‑1 (MQ=255)                                                                                                                                                                       cTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAGCTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAgg                                                                           >  1:86458/1‑251 (MQ=255)                                                                                                                                                                         tgtgGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAgg                                                                           <  1:338006/249‑1 (MQ=255)                                                                                                                                                                          gtgGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTACC‑TTTGAAGGCCGTATCCATCCGCTGGTTAACACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAAGATCAACCAGGCTTCTGAGCCCTTCGGCCCTGCTCTCAAAggggc                                                                        >  2:86953/1‑248 (MQ=255)                                                                                                                                                                           ggggATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGAt                                                                       <  2:463029/250‑1 (MQ=255)                                                                                                                                                                              gATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGCTCCTATCGAAACGGCAATAAAAAAAGGCGATTTAACCGTGGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCCACCGGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCg                                                                    >  1:242319/1‑251 (MQ=255)                                                                                                                                                                                 aCGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGtt                                                                 <  1:458101/251‑1 (MQ=255)                                                                                                                                                                                          ccaccTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGaaa                                                        <  1:161846/251‑1 (MQ=255)                                                                                                                                                                                            accTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGaa                                                         >  1:305998/1‑248 (MQ=255)                                                                                                                                                                                                gTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAAAGGCAATCAAAAAAGGCGATTTGACCGTCGGAGCGGTGCTGTCCCGCAACCG‑AACGTTTAAAGGCCGTATCCATCCGCTGGTTAAAAATAACTGGCTGGCCTGGCCGCCGCTGGGGGTTGCCTATGCGCTGGCGGGCAATATTCATAACAACCTGGCTTCTGAGCCTATAGGCCATGATCGCAAAGGCGACCCGGTTTATCttcaatatatt                                                  >  1:471264/1‑241 (MQ=255)                                                                                                                                                                                                   ttGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGACTATCGGCCATGATCGCACAGGCGATCCGGTTTATCTGGAAGATATC‑Tgg                                              >  2:129078/1‑251 (MQ=255)                                                                                                                                                                                                        aaCTCTGGAACGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCAAAAAAATGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGACATATGAATATCCACCGGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGGTTTTCTGGAAGAAATC‑TGGCCATc                                         >  2:239560/1‑251 (MQ=255)                                                                                                                                                                                                             tGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGGCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATC‑TGGCCATCGGcac                                    >  1:211273/1‑251 (MQ=255)                                                                                                                                                                                                                gCCGCTGCCCGATCCTATCGAAACGGAAATCAAAAAAGGCGATTAACCCGTCGGTGCGGTGCGGACCGGCAACCGTAGT‑TTTGAAGGCCGTAGCCATCCGCTGGTTAAAACTAACGGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATC‑TGGCCATCGGCACAAg                                 <  2:126720/251‑1 (MQ=255)                                                                                                                                                                                                                      accggATCCTATCGAAACGGCAATCAAAAAGGGCGATTTAACCGTCGAGGCGGTGCTATCCGGCACCCGTAAC‑TTTGAAGGCCGGATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCGGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATC‑TGGCCATCGGCACAAGAAATTg                           <  2:3980/247‑1 (MQ=255)                                                                                                                                                                                                                               tATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTACGGCAACCGTAAC‑TTTGAAGGCCGTCTCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATTATCGCAAAGGCGATCCGGGTTTTCTGAAAGATATCGTGGC‑ATCGGCCCCATAAATTTCCCGTGCgg                  >  2:456218/1‑251 (MQ=255)                                                                                                                                                                                                                                aTCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATC‑TGGCCATCGGCACAAGAAATTGCCCGTGCg                   <  1:370793/249‑1 (MQ=255)                                                                                                                                                                                                                                    aaaCGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTGGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATC‑TGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGaa             <  2:178987/251‑1 (MQ=255)                                                                                                                                                                                                                                       cGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATC‑TGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACaa          <  1:298586/251‑1 (MQ=255)                                                                                                                                                                                                                                              cAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATC‑TGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTcc    <  1:429805/250‑1 (MQ=255)                                                                                                                                                                                                                                                 aaaagGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATC‑TGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCcac  >  1:92213/1‑249 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       CCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAC‑TTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATC‑TGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCCAC  >  NC_000913/1337150‑1337638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑