Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	155,463	A→T	*247K (TAA→AAA)	yadV ←	putative fimbrial chaperone YadV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	155,463	0	A	T	100.0%	147.0 / NA	43	*247K (TAA→AAA)	yadV	putative fimbrial chaperone YadV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (21/22);  total (21/22)

GACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCA‑ATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAACCTG  >  NC_000913/155229‑155702                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                gACGTCTTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCA‑ATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATGCTATAGTCACGCTATGCTTCCTGCGGAATTAGATCCTTGCCTGATTTCAGCCTGGCATTAcc                                                                                                                                                                                                                                  <  1:68498/250‑1 (MQ=255) gACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCC‑ATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCt                                                                                                                                                                                                                                 >  2:13701/1‑251 (MQ=255)      cgtAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCA‑ATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAAt                                                                                                                                                                                                                            >  1:90405/1‑251 (MQ=255)       gtAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCA‑ATATTAGATGCATTCTGCCCCATCCGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAAtt                                                                                                                                                                                                                           >  2:322249/1‑251 (MQ=255)       gtAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCA‑ATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAAtt                                                                                                                                                                                                                           >  2:133523/1‑251 (MQ=255)         aaaCACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCA‑ATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGAGACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgc                                                                                                                                                                                                                         <  1:538268/251‑1 (MQ=255)         aaaCACCCGGTATAGCGGGGTGACCTTCACTGTACCGGCTGAGATCA‑ATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATTATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCGGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgc                                                                                                                                                                                                                         <  1:333153/251‑1 (MQ=255)                    ataGCGGGGTTACCTTCACTGTACCGGCTGAGATCC‑ATATTAGATGCATTCTGCCCCATCAGGAAGGTCTGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATCCAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAAtt                                                                                                                                                                                                                           >  2:70028/1‑238 (MQ=255)                    ataGCGGGGTTACCTTCACTGTACCGGCTGAGATCA‑ATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAAtt                                                                                                                                                                                                                           <  1:70028/238‑1 (MQ=255)                     tagaggggttacattcagagggacGGCTGAGA‑CACATATTAGATGAATTCTGCCCCCTCAGGAAGGTATGGTCATATTCACCAAGTTAAGCACTGAAAGCAGTATTGCACTACAGCGGCGCGCAAACCGTGGCGATACGGGTAAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCCGACTGGCATTACCTTCAATTGCGCCACCAAAGt                                                                                                                                                                                                              >  2:510813/24‑250 (MQ=255)                               aCCTTCACTGTACCGGCTGAGATCA‑ATATTAGATGCATTCTGCCACATCAGGAAGGTATGGTCATATTCACCGAGTTCACCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATCTCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCg                                                                                                                                                                                                   >  2:348350/1‑251 (MQ=255)                                ccTTCACTGTACCGGCTGAGATCA‑ATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGt                                                                                                                                                                                                  >  1:367365/1‑251 (MQ=255)                                    cACTGTACCGGCTGAGATCA‑ATATTAGATGCATTCTGCCCCAGCAGGAAGGTAGGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATGTCATCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaaaa                                                                                                                                                                                              <  1:445078/251‑1 (MQ=255)                                                 gagaTCA‑ATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGACGATAATTTGTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTg                                                                                                                                                                                   <  2:437764/249‑1 (MQ=255)                                                      cA‑ATATTAGATGCATTCCGCCACATAAGGAAGGTATGGTAATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAAtt                                                                                                                                                                            >  2:97554/1‑251 (MQ=255)                                                                 agcaatCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCa                                                                                                                                                                  <  2:442347/246‑1 (MQ=255)                                                                           cccATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCAt                                                                                                                                                        <  1:308285/251‑1 (MQ=255)                                                                             cATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTg                                                                                                                                                      <  1:348350/251‑1 (MQ=255)                                                                                cAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACTGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATGAATTGCGGAAAAATGCACTTTTGCAGAATTCTCTTTGCCATTAAGGCCATTGAc                                                                                                                                                    <  1:97554/250‑1 (MQ=255)                                                                                                  ttCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACt                                                                                                                                  >  1:80656/1‑250 (MQ=255)                                                                                                     aaCGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATGATATCCTTGCCTGAGTTCAGCCTGGCATTACCTTCAATTTCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTGTTTCAGAATTCTCTGGGCCATTAAGCCCAGGGACGTTCATGACCTCATCGCTAAAt                                                                                                                              <  2:507298/251‑1 (MQ=255)                                                                                                              aGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAATTGGTGCAATc                                                                                                                     >  1:211269/1‑251 (MQ=255)                                                                                                                      aaGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTcac                                                                                                             <  1:9371/251‑1 (MQ=255)                                                                                                                       aGCAGTATTGCAATCCAGCAGCGCGCACAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTcac                                                                                                             >  1:164219/1‑250 (MQ=255)                                                                                                                       aGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTcac                                                                                                             >  1:81025/1‑250 (MQ=255)                                                                                                                          aGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATACCCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTCCCTTCACTTTCGCCACCATAGTCATTAATGGCGTAAAAATGCCCTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCt                                                                                                                                         <  1:315926/219‑1 (MQ=255)                                                                                                                                      cagcagCGCGCAAAACGTGGAGATACGGGTCAGATGACGATAATTATCAGTATATTCTATAGTCACGATCTGCTTCCTGCGGAATTATCTCATTGCCAGATTTCAGCCTGGCATTACCTTCAATTGAGCCAACAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGTTTTGCCATTAAGGCCATTGACTTTCATGACCTAATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCgg                                                                                             >  1:334575/1‑250 (MQ=255)                                                                                                                                         cagcGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTa                                                                                          <  1:322249/251‑1 (MQ=255)                                                                                                                                          agcGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGc                                                                                                                         >  1:283715/1‑219 (MQ=255)                                                                                                                                          agcGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTAGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGc                                                                                                                         <  2:283715/219‑1 (MQ=255)                                                                                                                                                        ggCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTaaa                                                                           <  1:133523/251‑1 (MQ=255)                                                                                                                                                                gggTCAGATGCTGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTCCATCATTGCCTGATTTCAGCCTGCCATCCACTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAACTGCCCTTTTGCAGAATTCGCTTTGCCACTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACt                                                                   >  1:497429/1‑251 (MQ=255)                                                                                                                                                                 ggTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTg                                                                  <  1:209555/251‑1 (MQ=255)                                                                                                                                                                         gatgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGACTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACATCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTaaa                                                             >  2:437160/1‑248 (MQ=255)                                                                                                                                                                            gatAATTTTTAGTATCTTCTATAGTCACGCTATGCTTACTGCGGACTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGAt                                                                                                  >  2:322749/1‑208 (MQ=255)                                                                                                                                                                            gatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCGCATCACTAAATGGTGCAATCATTTTCACATCAATCGGAt                                                                                                  <  1:322749/208‑1 (MQ=255)                                                                                                                                                                                           atTCTATAGTCACGCTATGCTTCCTTCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACGTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAAGCGGATAGCGTTTACCGCTAGCTTCTAAATCTCCACTGCGAAAAGAGACGTAGTAAGGGGTTg                                         <  2:211269/250‑1 (MQ=255)                                                                                                                                                                                                   gTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCCGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTACGGGGTTGGATTGGTc                                 >  2:167201/1‑250 (MQ=255)                                                                                                                                                                                                    tCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCAc                               >  1:296143/1‑251 (MQ=255)                                                                                                                                                                                                        gCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGt                           <  1:167201/251‑1 (MQ=255)                                                                                                                                                                                                                    gccgAATTATATCCTTGCCTGATGTCAACCTGGCATTACCTTCAATTGCGCCACCACAGTCAGTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCGTTGCCATTAAGGCCATTGACTTTCATGACCTCAGCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTGCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCt                 <  2:300187/246‑1 (MQ=255)                                                                                                                                                                                                                        aaTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCtt           <  2:300028/251‑1 (MQ=255)                                                                                                                                                                                                                                 cTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAACCTg  >  1:41557/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                GACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCA‑ATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAACCTG  >  NC_000913/155229‑155702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑