Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,2680TC29.0% 27.5 / 20.2 31intergenic (+474/+168)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
Reads supporting (aligned to +/- strand):  ref base T (12/10);  new base C (4/5);  total (16/15)
Fisher's exact test for biased strand distribution p-value = 7.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.45e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑‑CGCATCCGACATCA‑ATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCG  >  NC_000913/4296023‑4296515
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                              
atggcgtgatggggttttACGCTGCAGCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCGTATGAGTCCTACGCGAGACAGCGCAATAGCCGGAGTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATTCCGCATCCGACGGCAACGCCGGATGCGACGCTTAATGCGTGTTATCAGGGCTACGCCAGACGGCTGAAGAGCCTGATGTAGCGTGATGTGGTAGGTCGGATAAGGGGTTTAcgccgc                                                                                                                                                                                                                                                           <  2:533761/236‑1 (MQ=2)
 ggTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCCATAGCCTGATTTCGCGTGATTTTGTAGGTCGGCTACGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACCCCAAACAGCGCATTAGCTTCCTTACGCGTGCTTTTGTCGGTCGTATAAGGCGTTTACGCCga                                                                                                                                                                                                                                                           >  2:141421/1‑249 (MQ=11)
    cGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATcc                                                                                                                                                                                                                                                       <  1:478653/251‑1 (MQ=37)
       aTAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGAc                                                                                                                                                                                                                                                    >  1:152909/1‑251 (MQ=38)
       aTAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGAc                                                                                                                                                                                                                                                    <  1:22818/251‑1 (MQ=38)
              gTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGAGTGATTTTGTAGGTCGGATAAAGCGTTTATGCCGCATCCGACATCAACGCCTGCTGCGACGCTTAACGCGTCTTATCAGGCCTACGCCCGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaatg                                                                                                                                                                                                                                             >  1:502435/1‑249 (MQ=255)
                 tgcgccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCAACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCg                                                                                                                                                                                                                                                            <  2:34916/231‑1 (MQ=18)
                 tACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCg                                                                                                                                                                                                                                                            >  1:34916/1‑233 (MQ=21)
                            cGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCt                                                                                                                                                                                                                               <  1:186248/251‑1 (MQ=17)
                            cGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCAGCCGACATCAATGCCTGATGCGACGCt                                                                                                                                                                                                                               <  1:213280/251‑1 (MQ=17)
                                                                                                                                                                          gcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGACTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCAGACATCA‑ATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAcc                                                                            >  1:491871/1‑251 (MQ=2)
                                                                                                                                                                                tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTGGCGTGATTTTGTAGGTCGGGTAAGGCGTTGATGCCGCATCCGACATCAACGCCTGATGCGACGCTGAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCA‑ATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGtt                                                                         <  1:355444/248‑1 (MQ=11)
                                                                                                                                                                                tATCAGGACTACGCCAGACAGCGCAATGGCCTGATGTAGCGTGATTTTGTAAGTCGGATAAGGCGTTTATGCCGCATCCGACATCAGCGCCTGATTCGACGCTTAACGCTTCTTATCAGGGCTACGCTAGACAGCGCAATTGCCTGATTTAGCGTGATTGTTTGTGTCGGATTTGGCGTGT‑‑ACGC‑‑CGCATCCGACAGTA‑ATGCCGTAGTCGTCGCTTGCCGCGTCTTATCAGCCCTATCTTAACCGTTg                                                                        <  1:48382/249‑1 (MQ=2)
                                                                                                                                                                                  ccgtgCCTACGCCAGACAGCGCAATAGACTGATTTAGCGTGATTTTGTAGGTCGGATAAGTCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTTATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCA‑ATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTa                                                                    <  2:229109/247‑1 (MQ=11)
                                                                                                                                                                                    aGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCA‑ATGCCTGCTGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAt                                                                  >  2:172284/1‑251 (MQ=255)
                                                                                                                                                                                            cctatctaGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCA‑ATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaa                                                           <  2:429721/243‑1 (MQ=255)
                                                                                                                                                                                                gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCA‑ATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAActct                                                      >  1:530984/1‑251 (MQ=255)
                                                                                                                                                                                                 aCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCA‑ATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCtt                                                     <  1:141421/251‑1 (MQ=255)
                                                                                                                                                                                                   agagcAATAGCCTGAGTTAGAGTGATTTTGTAGGTTGCATAAGGCGTTTATGCCGCATCGGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATAAGGCCTACGTCAGCCAGTGGTATCGCTTGCTTTAGCGCGATTTTGTGGGTCGGATAAGGCGTTTCCG‑‑C‑‑CGCCTCTGACA‑CATATGCCTGATGTGACGCTTGTCGCGTCTTATAAGGCCTATCTTAACCGTTGGTTCATTTTTCACACTCTTc                                                    >  2:288984/4‑250 (MQ=2)
                                                                                                                                                                                                   aGCGCTATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCTCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCA‑ATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGTCTATCTTAGCCGTTGGTTAATTTTTCAACCTCTTca                                                   <  1:414032/251‑1 (MQ=11)
                                                                                                                                                                                                   aGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCA‑ATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaa                                                           >  1:429721/1‑243 (MQ=255)
                                                                                                                                                                                                   aGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCA‑ATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc                                                    >  1:499847/1‑250 (MQ=255)
                                                                                                                                                                                                      gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACCTCA‑ATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCacac                                                >  1:358453/1‑251 (MQ=255)
                                                                                                                                                                                                             ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCA‑ATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTAt                                         <  1:172284/251‑1 (MQ=255)
                                                                                                                                                                                                                gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGCTTTAGCGTGATTTTGTACGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCA‑TTGACTGATGCGACGCTTGCCGCGTCTTTTCAGCCTTATCTTAACCGTTGGATAATTTTTCCAACTCTTCACACCCGGTATCa                                       >  2:57856/1‑250 (MQ=14)
                                                                                                                                                                                                                gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACCTCA‑ATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCa                                       >  1:13001/1‑250 (MQ=255)
                                                                                                                                                                                                                   ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCCGGCCTACGCCAGACAGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATACGGCGATT‑‑AAGC‑‑CGCATCTGACCTCA‑ATGCCAGATGCGACGCTTTCCGCCTCTTATCAGGCCTATCTTAAGCTTCGGTACATTTTTCCAACCCATCACACCCGGTATccagcc                                   >  2:248513/1‑246 (MQ=11)
                                                                                                                                                                                                                          aaaatagtagattggaaggaacGTTTATGCCGCAGCGTACACCAACGCCAGATACAACACATAACTCGTCTTATCACACCTACGCCATTCACCGCAAAATCCAAATATGGCGTGATTTTGTATGTCGTATAAGTCGTTT‑‑A‑‑CGACGTCCCCGACATCA‑ATATCTTAATCGACCCTTGCCGCGTCTTAGCGAGCCTATCATATCCGTTGTTTAATTTTTCAATCTCTTCACACCCGGTATCAAACCCTTCCAt                            <  1:50057/230‑1 (MQ=255)
                                                                                                                                                                                                                            ttttGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCA‑ATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATa                           <  2:146203/250‑1 (MQ=255)
                                                                                                                                                                                                                                       ggATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCA‑ATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAttttt                                                              >  2:108660/1‑204 (MQ=255)
                                                                                                                                                                                                                                       ggATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCA‑ATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAttttt                                                              <  1:108660/204‑1 (MQ=255)
                                                                                                                                                                                                                                                    aTGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACCGCGCCATAGCCTGACTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCAGACATCA‑ATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAgcg  >  1:328038/1‑251 (MQ=18)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                              
AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑‑CGCATCCGACATCA‑ATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCG  >  NC_000913/4296023‑4296515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑