| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,411,439 | 0 | A | C | 25.7% | 114.8 / 10.7 | 72 | G170G (GGT→GGG) | ttcA | tRNA cytosine(32) 2‑sulfurtransferase TtcA |
| Reads supporting (aligned to +/- strand): ref base A (36/16); new base C (0/18); total (36/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.50e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGACGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCAATCACCTGACGTTGCAGGTTAGGCTGTGAACCGCACAGGT‑TGCACGGAATAATCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCACGATACCGTAAGTATTCTCTTCAACAATCTTGTACTCAACGCCCAGCTTTTCAAGATACTCGGGCAGA > NC_000913/1411193‑1411685 | cGACGGCCCCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATCACGTTTATCACAGTCACGCAACATATCAGCAATCACCTGACGTTGCAGGTTAGGCTGTGAACCGCACAGGT‑TGCACGGAATACTCGGGAACGCTTTTGCC‑TCGGCAACTCGCTGAATCTCTTTCTCGAGGCAGTCGGCCAGCGGACGACTAACGATATGTTTTCCATACTCGCTCATCAGTTTCGGAGGC‑CTACCtt > 2:490335/1‑251 (MQ=255) aCGGCAACACATTATGCCTCCCGCTGAACATCGTCTCGATACGCCCTGGCTAACGTTTATCCCAGTCACGCAACCTGTCAGCAATCACCTGACGTTGCAGGTTAGGCTGTGAACCGCCCAGGT‑TGCCCGGAATAATCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGCAGGCCAGCGGCCGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑CTACCTTTc > 2:242161/1‑251 (MQ=255) gCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCAATCACCTGACGTTGCAGGTTAGGCTGTGAACCGCACAGGT‑TGCACGGAATAATCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATc > 1:351038/1‑251 (MQ=255) accacATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCAATCACCTGACGTTGCAGGTTAGGCTGTGAACCGCACAGGT‑TGCACGGAATAATCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGTTATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCtt > 2:174626/1‑251 (MQ=255) accacATTCTGCATCGCGCTGAACATCGTCTCGATACCCCATGCATAACGTTTATCCCAGTCACGCACCATGTCAGCAATCACCTGACGTTGCAGGTTAGGCTGTGAACCGCACAGGT‑TGCACGGAATAATCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCtt > 1:43847/1‑251 (MQ=255) cATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCAATCCCCTGACGTTGCAGGTTAGGCTGTGAACCGCACAGGT‑TGCACGGAATAACCGGGACCGCTTTTGCA‑TCGGCAAATCGCTGAATATTTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCAGCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGa < 1:37749/251‑1 (MQ=255) gcTGAACCTCGTCTCGATACGCCCTGGATAACGTTTATATCAGTCACGCAACATGTCAGCAATCACCTGACGTTGAAGGTTAGGCTGTGCCCCGCCCAGAT‑CGCACGGAATAATCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATATTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAgcacttct > 1:218267/1‑244 (MQ=255) aaCATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCAATCACCTGACGTTGCAGGTTAGGCTGTGAACCGCACAGGT‑TGCACGGAATAATCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGCGGC‑ATACCTTTCATCTTACCGCCGTCGAACATATTTa > 2:167648/1‑250 (MQ=255) aCATCGTCTCGATACGCCCGGGATAACTTTTATCCCAGTCACGCAACATGTCAGCAAGCACCTGCCGTTGCAGGTTAGGCGGTGAACCGCACAGGT‑TGCACGGAATAATCGGGAACGCTTGGGCA‑TCGGCAAATCGCTGAATATCTTTCGCCCGGCAGTAGGCCGGCGGACGAATAACGATATGTGTGCTATCATCGCTCATCAGTTTCGGGGAC‑ATCCCTTTCTTCTTACCGCCGTAGAACATATTTAAg < 1:226016/251‑1 (MQ=255) gTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCAATCACCTGACGTTGCAGGTTAGGCTGTGAACCGCACAGGT‑TGCACGGAATAATCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGaaca > 1:261174/1‑250 (MQ=255) aTACGCCCTGGATAACTTTTACCCCAGTCACGCAACATGGCAGCAATCCCCTGACGTTGCAGGTGAGGCTGTGAACCGCACAGGT‑TGCACGGAATAATCGGGACCGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCATTAGGCCAGCGGACGAATAACGATATGTTTCCCATCATCGCTAATCATTTCCGGAGGC‑ATTCCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTg < 2:351038/251‑1 (MQ=255) aCGCCCTGTATAACTTTTATCCCAGTAACGCAACATGACATCAAGCACCTGGCGTTGCTGGTTGGGCTGTGACCCACACAGGT‑GGCACTGAAAAATCGGGAACTTTTGTGCA‑TCGGAAAAGCGTTGAATAGTTTGCTCGCTGCAGTAGGCCAGGGGAGAAATACCGATATTTTTGCCAGCATCGCTCATCAGTTACGGATGC‑ATACTTAACACCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGc < 1:480642/250‑1 (MQ=255) cGCCCTGGATAACGTTTATCCCAGTCACGCACCATGTCAGCAATCACCTGACGTTGCAGGTTAGGCTGTGAACCGCACAGGT‑TGCACGGAATAATCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGc > 2:151351/1‑249 (MQ=255) gATAACGTTTATCCCAGTCACGCAACATGTCAGCAATCACCTGACGTTGCAGGTTAGGCTGTGAACCGCACAGGT‑TGCACGGAATAATCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCg > 1:31895/1‑251 (MQ=255) aaaCGTTTATCCCGGTCCCGCAACATGCCAGGAATCACCGGCCGTTGCAGGTTGGGCTGTGAGCCGAACAGGT‑TGAACGAAATAATCGGGAACGCTTTTGCA‑TGGGAAAATCGCGGAATATCTTTCTCGCGGCATTAGGCCAGCGGACGAATAACGATTTGTTTGCCACCATCGCTCATCAGTTTCGGGGGC‑AGCCCTTTCTGCTTTCCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTc < 2:438885/250‑1 (MQ=255) cGTTTCTCCCAGTCACGCAACATGTCAGCAATCACCTGACGTTGCAGGTTAGGCTGTGAACCGCACAGGC‑TGCACGGAATAATCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACg > 2:187725/1‑251 (MQ=255) gTTTATCCCCGTCACGCAACATGTCAGCAATCACCTGACGTTGCAGGTTAGGCTGTGAACCGCACAGGT‑TGCACGGAATAATCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACg > 1:189336/1‑250 (MQ=255) ttATCCCAGTCACGCAACATGACAGAAATCACCTGACGTTGCAGGTGAGTCGGTGACCCGCAAAGGT‑TGCACGGAATAATCGGGAACGCTTTTGCA‑TCGGAAAATCGCTGAATATCTTTCGCGCGGCAGGAGCCCAGCGGACGAATAACTAGATGTTTGCCATTATGGCATATCGGTGGCGGGGGC‑TTCCCTTTCATTTTTCCGCCGTAGAACATATGTAAGAACAACGTTTGCAGGATATCGTCACGAt < 2:206876/250‑1 (MQ=255) aTCCCAGTCACGAAACATGTCAGCAATCACCTGACGTTGCAGGTTAGGCTGTGAACCGCACAGGT‑TGCACTGAATAATCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCGCGCGGCAGTAGGCCAGCGGACGAATAACAATATGTTTGCCATCAGCGCTCACCAGTTTGGGAGGC‑ATACCTTTCATCTGACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGAATATTGTCACGATGGt < 1:187725/251‑1 (MQ=255) aGTCACGCAACAGGTCAGCAAACACCGGACGTGGAAGGTGAGGCTGGGAACCGCACAGGT‑TGCAAGGAATATGCGGGAAGGCTTTTGCA‑GCGGCAAATCGCTGAATATCTTTCTCGCGGAAGTAGGCCAGCGGACAAAAAACTATATGTTGGCCGTCATCGCTCATAAGTTTCGGGGGC‑ATACCTTTCATCTTACCGCCGGAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGAccc < 1:348809/251‑1 (MQ=255) aCATGTCAGCAATCACCTGACGTTGCAGGTTAGGTTGTGAACCGCACAGGT‑TGCACGGAATAATCGGGAACCCTTTTGCA‑TCGGAAAATCGCTGAATATCTTTCGCGCGGCAGTAGGCCAGCGGACAAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATc < 1:161159/251‑1 (MQ=255) aGCAATAACCTGACGTTGCAGGTTAGGCTGTGAACCGCACAGGT‑TGCACGGAATAATCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTtcgtcg > 2:254201/1‑251 (MQ=255) tCACCTGACGTTGCAGGTTAGGCTGTGAACCGAACAGCT‑TGAACGGAATAATAGGAAACGCTTTTGCA‑TCGGCAACTCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTTCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACCACGTTTGCAGGATATCGGCACGATGGTGAACCAACGCGCTCCTCgccgcctcc > 2:282195/1‑248 (MQ=255) cACCTGACGTTGCAGGTTAGGCTGTGAACCGCACAGGT‑TGCACGGAATAATCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGccccc > 2:174823/1‑250 (MQ=255) gACGTTGCAGGTTAGGCTGTGAACCGCACAGGT‑TGCACGGAATAATCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTcc < 1:254201/251‑1 (MQ=255) gtttGCAGGTTGGACTGTGAACCGCA‑AGGTGTGCCCAGAATAATCGGGAACGCTTGT‑CACTCGCCAAATCGCTGAATCTCTTTCGCGCGGCAGACGGCCAGCGGACGAATACCTATATGTTTGCCATCATCGATCATCATTTTCGGAGGC‑ATCCCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGt < 2:117137/249‑1 (MQ=255) ggTTAGGCTGTGAACCGCCCAGGT‑TGAACGGAATAATCGGGAACGTTTTTGCA‑TCGCCAAATCGTTGAATATCTTGCTCGCGGCAGTGGGCCAGCGAACAAATAACGATATTTGTTCCATCATCGCTCATCATTTTCGGAGGC‑TTCCCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGAGCTTCGTCGCCCCCAGTTCCGTTGCGGTa < 2:277202/251‑1 (MQ=255) gTTAGGCTGTGAACCGCACAGGT‑TGCACGGAATAATCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTAc > 2:389743/1‑251 (MQ=255) gTTAGGCTGTGAACCGCACAGGT‑TGCACGGAATAATCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTAc > 1:306739/1‑251 (MQ=255) tAGGCTGTGAACCGCATAGGT‑GGCACGGAATAATCGGGAACGCTTTTGCA‑TCGGCAAACCGCTGAATATCTTTCCCGCGGCAGTAGGCCAGCGGACGATTAGCGATAGGTTTGCCATCATCGCTCATCGGTTCCGGAGGC‑AATCCTTTCATCTTTCCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGTTGTCCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACg < 1:271619/250‑1 (MQ=255) gtgAACCGCACAGGT‑TGCACGGAATAATCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAgg > 1:446019/1‑251 (MQ=255) ccGCACAGGT‑TGCACGGAATAATCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGGATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATAcc > 1:348477/1‑251 (MQ=255) gCACAGGT‑TGCACGGAATAATCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGCTATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTGGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCC‑GTTCCGTTGGGGTACGATAAAGGCTACcgcg > 2:289082/1‑251 (MQ=255) caGGT‑TGCAGGAAATAATCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCCCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGcgac < 1:78826/251‑1 (MQ=255) caGGT‑TGCACGGAATAATGGGGAACGCTTTGGCA‑TCGGCAAATCGCTGAATTTCTTTCCCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACTTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGcgac < 1:208098/251‑1 (MQ=255) gCACGGAATAATGGGGACCGCTTTTGCA‑CCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACAAATAACGATATTTTTGCCATCACCGCTCATCAGTTTCGGAGGC‑ATCCCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGTTACGATAAAGGATACCGCGACGAAGGc < 1:459778/251‑1 (MQ=255) gCACGGAATAACCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCATTAGGCCAGCGGCCTAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATCCCTTTCATCTTGCCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAgg < 1:423106/250‑1 (MQ=255) cACGGAATAATCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCg > 1:527674/1‑251 (MQ=255) aataatCGGAAACGCTTTTGCA‑TCGGCAAATCGCTGAAGTTCTTTCTCGGGGCAGTAGGCCAGCGGACGAATAACGATATTTTTGCCATCATCCCTCATCAGTTTCGGAGGC‑ATCCCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAac < 2:118522/251‑1 (MQ=255) aaaaatCGGGAACGCTTTTGAA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATCCCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAac < 1:174823/248‑1 (MQ=255) ataatcggaaccctgtttGCA‑TCGGAAAATCGCTAAATTTCTTTTCCGCGGCAGTAGGCCAGCGGACTAAGAGCGATAGGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑GTACCTTTCACCTGACCGCCGTAGAACATATTTAAGAACAACGTGTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCTTCGCCCGCAGGTCCGTTGCGGTACGATAAAGTATACCGCGACGAAGGTGAGAaca < 2:348477/236‑1 (MQ=255) aatCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAacac > 1:448301/1‑250 (MQ=255) ggAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGAGGCCGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTAATCAGTTTCGGACGC‑ATACCTTTCCTCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGCTCTTCGTCGCCCCCAGTTCCGTTGCGGAACGACAAAGGATACCGCGACGACGGCGAGAACACAGTg > 1:257435/1‑249 (MQ=255) ttGCA‑TCGGCAAATCGCGAAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATCCCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGt < 1:31458/250‑1 (MQ=255) gCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATCCCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGttt < 1:108874/250‑1 (MQ=255) gCA‑TCGGAAAATCGCTGAATATCTTTCCCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATCCCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGttt < 1:466312/250‑1 (MQ=255) a‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGACAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGCTATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGc > 1:439940/1‑251 (MQ=255) ggCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCct > 2:397237/1‑251 (MQ=255) aaaTCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATTTTTGCAATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTg < 1:375120/251‑1 (MQ=255) cgcggaatttcttgcccgcgGAAGTGGGCCAGCGGAACAAAAACTATATGTTTGCCATCACCCCTCATCAGTTTCGGAGTC‑ATCCCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAAt < 2:123586/235‑1 (MQ=255) cGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATTTGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGaa < 1:533304/250‑1 (MQ=255) cGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGGTTTGCCCTCTGGAAt > 2:304656/1‑251 (MQ=255) atCTTTCTCGCGGAAGTAGGCCAGCGGACGAATAACGATATTTTTGCCATCTTCGCTCATCATTTTCGGAGGC‑ATCCCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACTCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTctct < 1:314904/250‑1 (MQ=255) tttttCCCGCGGCAGTGGGCCAGCGAACAAATAACGATATTTTTGCCATCATCGCTCATCAGTTTCGGGGGA‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCtt < 1:151351/248‑1 (MQ=255) ctcGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCACg > 1:65052/1‑250 (MQ=255) ccgcgGCAGTAGGCCAGCGGACAAATAACGATATGTTTGCCATCATCCCTCATCAGTTTCGGAGGC‑ATCCCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCACGAt < 1:389743/250‑1 (MQ=255) gcgGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCACGATAc > 1:266995/1‑251 (MQ=255) ggCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATCCCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCACGATACCg < 1:190459/251‑1 (MQ=255) cAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCACGATACCGt > 1:115448/1‑250 (MQ=255) aGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCACGATACCGTAAGTa < 1:304656/251‑1 (MQ=255) gCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCAAGTTCCGTTGCGGTCCGATAAAGGCTACCGCGCCGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGACCTCTGGAATCTTCTCTTTCACGATACCGTAAGTAtt > 2:471546/1‑251 (MQ=255) cAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCACGATACCGTAAGTATtct > 1:82877/1‑251 (MQ=255) aGCGGATGAATAACGATATGTTTGCCATCATCGCTCATAAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCGACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCACGATACCGTAAGTATtctc > 2:354835/1‑251 (MQ=255) tGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCACGATACCGTAAGTATTCTCTTCAACAATCTTGTACTCAAc < 1:167648/250‑1 (MQ=255) gCCATCATCGCTACCCGGTTTCG‑AGGCCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCACGATACCGTAAGTATTCTCTTCAACAATCTTGTACTCAACGc < 2:402385/251‑1 (MQ=255) tcatcaGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCACGATACCGTAAGTATTCTCTTCAACAATCTTGTACTCAACGCCCAGCTTTTc > 2:168489/1‑250 (MQ=255) catcaGTTTCGGAGGA‑AACCCTGTAAGCTTCCCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAAGCTTCTCTTTCACGATACCGTAAGTATTCTCTTCAACAATCTTGTACTCAACGCCCAGCTTTTc < 1:236404/249‑1 (MQ=255) atcaGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCACGATACCGTAAGTATTCTCTTCAACAATCTTGTACTCAACGCCCAGCTTTTCa > 2:162181/1‑249 (MQ=255) cggttccGGAGGC‑ATCCCTTACATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCACGATACCGTAAGTATTCTCTTCAACAATCTTGTACTCAACGCCCAGCTTTTCAAGAt < 2:261174/245‑1 (MQ=255) caGTTTCGGAGGC‑ATACCTTTCTTCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCACGATACCGTAAGTATTCTCTTCAACAATCTTGTACTCAACGCCCAGCTTTTCAAGa < 1:236911/250‑1 (MQ=255) aGGC‑ATACCTTTCATCTTACCGCCGTAGAACATCTTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCACGATACCGTACGTATTCTCTTCAACAATCTTGTACTCAACGCCCAGCTTTTCAAGATACTCGGGc > 2:45322/1‑250 (MQ=255) c‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGCACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCACGATACCGTAAGTATTCTCTTCAACAATCTTGTACTCAACGCCCAGCTTTTCAAGATACTCGGGCAGa > 1:321916/1‑250 (MQ=255) | CGACGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCAATCACCTGACGTTGCAGGTTAGGCTGTGAACCGCACAGGT‑TGCACGGAATAATCGGGAACGCTTTTGCA‑TCGGCAAATCGCTGAATATCTTTCTCGCGGCAGTAGGCCAGCGGACGAATAACGATATGTTTGCCATCATCGCTCATCAGTTTCGGAGGC‑ATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCACGATACCGTAAGTATTCTCTTCAACAATCTTGTACTCAACGCCCAGCTTTTCAAGATACTCGGGCAGA > NC_000913/1411193‑1411685 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |