Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,890,411	IS1 (–) +9 bp	coding (176‑184/1248 nt)	tnaB →	tryptophan:H(+) symporter TnaB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1979270	NA (NA)	47 (0.910)	43/488	0.3	97.8%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	3890411 =	1 (0.020)					coding (176/1248 nt)	tnaB	tryptophan:H(+) symporter TnaB
*	?	NC_000913	= 3584195	NA (NA)	41 (0.790)	38/488	0.4	97.5%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	= 3890419	1 (0.020)					coding (184/1248 nt)	tnaB	tryptophan:H(+) symporter TnaB

CATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAA‑ACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATA  >  NC_000913/1979039‑1979289                                                                                                                                                                                                                                         |                    cATATCACTGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTACGTGAAATGCCGTTGCCATGTTTTCCGGCAGTGAGAGCAGGGCTAGCGCTGATGTCCGGCGGTGCTTTTGACGTTACGCCCCACCCCCTCAGTAGCTGGACAGGAGGGACAGCTGATCGGAAAAGAAGCCACTGGTGGCCCTCAAAAGAAACC‑TCATCCACCAAATCCGTACGTTGGCAGCATCAcccgcttaaaaatagtttcac  >  2:35156/1‑233 (MQ=2)                                                                                                                                                                                                                                         |                    CATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAA‑ACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATA  >  NC_000913/1979039‑1979289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.