Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,256,0610CT22.4% 98.9 / 14.3 58E136K (GAA→AAA) plsBglycerol‑3‑phosphate 1‑O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (10/35);  new base T (13/0);  total (23/35)
Fisher's exact test for biased strand distribution p-value = 3.63e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGT‑GCCGTGTT‑C‑ATCCGCCA‑TACGGCGCAGCGAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTTCGCCTTTTTCACGCCCCGGCGCGCG‑AC‑CAAA‑CAT‑CACCGACACTGGCACCATCTGCACATCCA‑G‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGT‑CGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGT  >  NC_000913/4255812‑4256304
                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                          
tattaAACAGATCGTGACGAGCGGGCAAACGTGGGCCTACGGCAGCCAGACTTTGACGGGCAAAGGGCATACGCGCCCCGCGCCCCAGTTTCTGATCG‑ATAGTTTTATCCGG‑GCGGTGTT‑C‑TGCCGCCA‑TACGGCGCAGCGAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTTCGCCTTTTTc                                                                                                                                                                                                                                                            <  2:466351/250‑1 (MQ=255)
     aaCAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGT‑GCCGTGTT‑C‑ATCCGCCA‑TACGGCGCAGCGAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTTCGCCTTTTTCACGccc                                                                                                                                                                                                                                                      <  1:261022/251‑1 (MQ=255)
                aCGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGT‑GCCGTGTT‑C‑ATCCGCCA‑TACGGCGCAGCGAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTT‑ACTTCTCCCTTTTTACGCCCCCGCGCGCG‑Ac                                                                                                                                                                                                                                          >  2:418418/1‑251 (MQ=255)
                 cGAGCAGGAAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGT‑GCCGTGTT‑C‑ATCCGCCA‑TACGGCGCAGCGAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTTCGCCTTTTTCACGCCCCGGCGCGCG‑Ac‑c                                                                                                                                                                                                                                        <  1:56350/251‑1 (MQ=255)
                    gCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGAAAAGTGCATACGCGCCACGCGCGCCAGTTTATGAGCG‑ATAGTTTTATCCGT‑GCCGTGTT‑C‑ATCCGCCA‑TACGGCGCAGCGAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTT‑ACTTAGCCTTTTTCACGCCCCCGCCCGCG‑AC‑Cac                                                                                                                                                                                                                                      >  1:200646/1‑249 (MQ=255)
                             cGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGT‑GCCGTGTT‑C‑ATCCGCCA‑TACGGCGCAGCGAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTTCGCCTTTTTTACGCCCCCGCGCGCG‑AC‑CAAA‑CAA‑CACCgc                                                                                                                                                                                                                          >  1:174150/1‑250 (MQ=255)
                                  gCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGT‑GCCGTGTT‑C‑ATCCGCCA‑TACGGCGCAGCGAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCGATTTCCCCTTTTTCCCCCCCCgggccgcgccacacaaaacccccacct                                                                                                                                                                                                                          >  2:315603/1‑226 (MQ=255)
                                         gCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCACCAGTTTCTGAGCG‑ATAGTTTTATCCGT‑GCCGTGTT‑C‑ATCCGCCA‑TACGGCGCAGCGAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTT‑ACTTCTCCTTTTTTACGCCCCGGCGCGCG‑GC‑CAAA‑CAT‑CAACCACACTGGccacat                                                                                                                                                                                                              >  1:224801/1‑246 (MQ=255)
                                          cAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGT‑GCCGTGTT‑C‑ATCCGCCA‑TACGGCGCAGCGAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTTCGCCTTTTTTACGCCCCCGCGCGCGCAC‑CAAA‑‑AC‑CACCGACACTggacccctc                                                                                                                                                                                                             >  1:86463/1‑244 (MQ=255)
                                           aGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGT‑GCCGTGTT‑C‑ATCCGCCA‑TACGGCGCAGCGAAAC‑TGACGGAGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTTAGCATACGCAGCGGCGGGGTC‑ACTTTACCCTTTTTACCCCCCCGCGCGCG‑AC‑CCAA‑CAT‑ccaccaccctcgcccccacc                                                                                                                                                                                                            >  1:194371/1‑232 (MQ=255)
                                              cAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGT‑GCCGTGTT‑C‑ATCCGCCA‑TACGGCGCAGCGAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTGC‑ACCTCGCCTTTTTTTCCCCCCCGCGCCCG‑GC‑CAAA‑CAT‑CAcccccacccgaccccccatcc                                                                                                                                                                                                         >  2:353363/1‑232 (MQ=255)
                                                     ggACGGGAAAAGTGCAAACGGCCCACGCGCGCCGTTTTCTGAGCG‑TTGGTTTTACCCGT‑GCCGTTTC‑C‑ACCCGCCA‑TACGGCGCAGCAAAAC‑TGACGGGGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCTTTAAGCATACGCAGCGGCGGGTTC‑ACTTCGCCTTTTTCACGCCCCGGCGCGCG‑AC‑CAAA‑CAT‑CACCGACACTGGCACCATCTGCACATCCa                                                                                                                                                                                                   <  2:232036/249‑1 (MQ=255)
                                                             aaaGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGT‑GCCGTGTT‑C‑ATCCGCCA‑TACGGCGCAGCGAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTC‑ACTTCTCCCTTTTCACGCCCCCGCGCGCG‑GC‑CCAA‑CAT‑CACCCACCCTTGCACCCTCTGCCCATCCacaattgtgg                                                                                                                                                                                          >  1:137191/1‑242 (MQ=255)
                                                             aaaGTGCAGGCGCGCCAGGCGCGCCATTTTCGGAGCG‑ATAGTTTTATCCTG‑GCCGTTTT‑C‑ACCCGCCA‑TGCGGCGCAGCGAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCAGACGCGGCGGCTGGTTC‑ACTTCGCCTTTTTCTCGCCCCGGCGCGCG‑AC‑CAAA‑TGT‑CGCCGACACTGGCATCATCTGCACATCCA‑G‑ATTTGGGt                                                                                                                                                                                        <  2:591926/251‑1 (MQ=255)
                                                              aattGCACACGCGCCCCGCGCCCCAGTGTCGGGGAG‑AAAGTTTTACCCGT‑GCAGTGT‑‑A‑AACCTCCAATACGGCGCAGCGAAAC‑TGACGGCGAGAAACGCAAAAACCTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTTAGCATACGCAGCGGCGGGTTC‑ACTTCGCCTTTTTCACGCCCCGGCGCGCT‑AC‑CAAA‑CAT‑CACCGACACTGGCACCATCTGCACATCCA‑G‑ATTTGGGtt                                                                                                                                                                                       <  2:194371/248‑1 (MQ=255)
                                                                gtgaaaccgcgcccggcgcgcCAGTTTCTGAGCG‑AAAGTTTTATCCGT‑GCCGTGTT‑C‑ATCCGCCA‑TACGGGGCAGCGAAAC‑TGACGGCGAGAAACGCACAAAGCTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTTCGCCTTTTTCACGCCCCGGCGCGCG‑AC‑CAAA‑CAT‑GACCGACACTGGCACCATCTGCACATCCA‑G‑ATTTGGGTTGc                                                                                                                                                                                     <  1:31024/236‑1 (MQ=255)
                                                                 ggCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGG‑GCCGTGTT‑C‑ATCCGCCA‑TACGGCGCAGCGAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCTGCGGGTTC‑ACTTCGCCTTTTTCACGCCCCGGCGCGCG‑AC‑CAAA‑CAG‑CACCGACACTGGCACCATCTGCACATCCA‑G‑ATTTGGGTTGCt                                                                                                                                                                                    <  1:556376/250‑1 (MQ=255)
                                                                   catccgcgCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGT‑GCCGTGTT‑C‑ATCCGCCA‑TACGGCGCAGCGAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTTCGCCTTTTTCACGCCCCGGCGCGCG‑AC‑CAAA‑CAT‑CACCGACACTGGCACCATCTGCACATCCA‑G‑ATTTGGGTTGCTAc                                                                                                                                                                                  <  1:52895/247‑1 (MQ=255)
                                                                     gaCGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGT‑GCCGGGTT‑C‑ATCCGCCA‑TACGGCGCAGCGAAAC‑TGGCGGAGAGAAACGCACAAAACTGGCGCGCCCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATGCGCAGCGGCGGGTTC‑ACTTCGCCTTTTTCACGCCCCGGCGCGCG‑AC‑CAAT‑CAT‑CACCGACACTGGCACCATCTGCACATCCA‑G‑ATTTGGGTTGCTACgg                                                                                                                                                                                <  1:33497/250‑1 (MQ=255)
                                                                             gcgcgcgCCAGTTTTGGAGCG‑ATATTTTTA‑ACGTCGCGGT‑TT‑CTATCCCCCA‑TACGGCGCAGCGAAAC‑TGCCGGCGAGAAACGCACAAACCTGTCTCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTTCGCCTTTTTCACGCCCCGGCGCGCG‑AC‑CAAA‑CAT‑CACCGACACTGGCACCATCTGCACATCCA‑G‑ATTTGGGTTGCTACGGTGCAAATc                                                                                                                                                                        <  2:253415/250‑1 (MQ=255)
                                                                              cgcgcgCCAGTTTC‑GAGGGGATATTTTTATCCGT‑GCCGTGTT‑C‑ACCCGCCA‑TACGGCGCAGCAAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTTCGCCTGTTTCACGCCCCTGCGCGCG‑AC‑CAAA‑CAT‑CACCGACTCTGGCACCAGCTGCACAGCCA‑G‑ATTTGGGTTTCTACGGTGCAAATCg                                                                                                                                                                       <  2:376002/251‑1 (MQ=255)
                                                                                   cgccagtgtcgggtcgttggTTTTACCCGT‑GCC‑TGTTTC‑ATCCGCCT‑TAGGGCGCAGGGAAAC‑TGTCGGCGAAAAACCCACAAAACGGTGGCGACTGAGCCACAGTACAGCGAAAAATTTTTGTACGCCGTTAAGCATACGCAGCGGCGGGTGC‑ACTTCGCCTGTTTCACGCCCCGGCGCGCG‑AC‑CAAA‑CAT‑CACCGACACTGGCACCATCTGCACAACCA‑G‑AGTTGGGTTGCTACGGTGCAAATCGAGAt                                                                                                                                                                   <  2:83365/232‑1 (MQ=255)
                                                                                   cccattttCTGAGCG‑ATAGTTTTATCCGT‑GCCGTGTT‑C‑TCCCGCCA‑TCCGGCGCAGCGAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTTCGCCTTTTTCACGCCCCGGCGCGCG‑AC‑CAAA‑CAT‑CACCGACACTGGCACCATCTGCACATCCA‑G‑ATTTGGGTTGCTACGGTGCAAATCGAGATa                                                                                                                                                                  <  1:340217/246‑1 (MQ=255)
                                                                                         gttgcggaggaagagttgtacccgggccgggTT‑C‑ACCCGCCA‑TACGGCGCAGCGAAAC‑GGACGGCGAAAAACGCACAAAACTGTCCCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTGCGCCTTTTTCACGCCCCGGCGCGCG‑AC‑CAAA‑CAT‑CACCGACACTGGCACCATCTGCACATCCA‑G‑ATTGGGGTTGCTACGTGGCAAATCGAGATAGT‑Cg                                                                                                                                                             <  2:86186/226‑1 (MQ=255)
                                                                                            tGAGCG‑ATAGTTTTATCCGT‑GCCGTGTT‑C‑ATCCGCCA‑TACGGCGCAGCGAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTC‑CCTTCGCCTTTTTCACGCCCCCGCGCGGG‑AC‑CCAA‑CAT‑CACCGACACCGGCCCCACCTCCCCATCCC‑GCTTTTGGGTT‑CTACCGTGCCAATCGCGATAAT‑CGTGGa                                                                                                                                                         >  1:283581/1‑250 (MQ=255)
                                                                                                     ggTTTTACCCGT‑GCCGGGTT‑G‑ACCCGCCA‑TCCGGCTCAGCGAAAC‑GGACGGCGAAAAACGCACAAAACTGTCGCTCCCGAGCCACAGTACAGCGAAAAATTTCTGTACCCCTTTAAGCATCCGCAGCGGCGGGTCC‑ACTTCGCCTTTTTCACGCCCCTGCGCGCG‑AC‑CAAA‑CAT‑CACCGACACTGGCACCATCTTCACAGCCA‑G‑ATGTGGGTTGCTACGGTGCAAATCGAGATAGT‑CGTGGAACAGCTTa                                                                                                                                                 <  1:222068/249‑1 (MQ=255)
                                                                                                     aGTTTTACCCGT‑GCCGTGTT‑C‑ATCCGCCA‑TACGGCGCAGCGAAACGTG‑CGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACTGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTTCGCCTTTTTCACGCCCCGGCGCGCG‑AC‑CAAA‑CAT‑CACCGACACTGGCACCATCTGCACATCCA‑G‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGT‑CGTGGAACAGCTTa                                                                                                                                                 <  1:449470/250‑1 (MQ=255)
                                                                                                            ccggt‑gccgtgTC‑C‑ACCCGCCA‑AGCGGCGCAGGGAATC‑TGACGGGGAAAAACGCACAAAACGGCCGCGACCGAGCCACAGTACAGCGAAAAATTTTTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTGGGCCTTTTTCACGCCCCGGCTCGCG‑AC‑CAAA‑GAT‑CACCGACACTGGCTCCATCTGTTCATCCA‑G‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGT‑CGTTTAACAGCTTAATAGActc                                                                                                                                         <  2:86181/248‑1 (MQ=255)
                                                                                                             ccgt‑gccgtgTT‑C‑ATCCGCCA‑TACGGCGCAGCGAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCCCAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTTCGCCTTTTTCACGCCCCGGCGCGCG‑AC‑CAAA‑CAT‑CCCCGACACTGGCACCATCTGCACATCCG‑G‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGT‑CGTGGAACAGCTTAATAGActct                                                                                                                                        <  1:419164/251‑1 (MQ=255)
                                                                                                             ccgt‑gccgtgTT‑C‑ATCCGCCA‑TACGGCGCAGCGAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTT‑ACTTCTCCTTTTTCTCCCCCCGGCGCCCC‑AC‑CCAA‑CCT‑CACCCCCAATGGCACCCTCTGCCCATCCC‑C‑CTTTGGGGTTCTACCGTGCAAATCGCGGTAGT‑CGGGGAAAAGCCTAATAGAatct                                                                                                                                        >  2:502491/1‑251 (MQ=255)
                                                                                                             ccgt‑gccgtgTT‑C‑ACCCGCCA‑TACGGCGCAGCGAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑TCTTCGCCTTTTTCACGCCCCGGCGCGCG‑AC‑CAAA‑CAT‑CACCGACACTGGCACCATCTGCACATCCA‑G‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGT‑CGTGGAACAGCTTAATAGActct                                                                                                                                        <  1:55865/251‑1 (MQ=255)
                                                                                                              cgt‑gccgtgTT‑C‑ATCCGCCA‑TACGGCGCAGCGAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTTCGCCTTTTTCACGCCCCGGCGCGCG‑AC‑CAAA‑CAT‑CACCGACACTGGCACCATCTGCACATCCA‑G‑ATTTGGGTTGc                                                                                                                                                                                     <  1:7816/206‑1 (MQ=255)
                                                                                                                 gcccgtgTT‑C‑ATCCGCCA‑TACGGCCCAGCGAAAC‑GGACGGCGAGAAACGCACAAAACTGTCGCGCCCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTTCGCCTTTTTCACGCCCCGGCGCGCG‑AC‑CAAA‑CAT‑CCCCGACACTGGCACCATCTGCACAGCCA‑G‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGT‑CGTGGAACAGCTTAATAGACtcttct                                                                                                                                     <  2:545306/249‑1 (MQ=255)
                                                                                                                   ccgtgTT‑C‑ATCCGCCA‑TACGGCTCAGCGAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTTCGCCTTTTTCGCGCCCCGGCGCGCG‑AC‑CAAA‑CAT‑CACCGACACTGGCACCATCTGCGCATCCA‑G‑ATTTGGGTTGCTACTGTGCAAATCGAGATAGT‑CGTGGAACAGCTTAATAGACTCTTCttt                                                                                                                                   <  1:274587/251‑1 (MQ=255)
                                                                                                                   ccgtgTT‑C‑ATCCGCCA‑TACGGCGCAGCGAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTTCGCCTTTTTCACGCCCCGGCGCGCG‑AC‑CAAA‑CAT‑CACCGACACTGGCACCATCTGCACATCCA‑G‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGT‑CGTGGAACAGCTTAATAGACTCTTCttt                                                                                                                                   <  1:430934/251‑1 (MQ=255)
                                                                                                                           c‑ATCCGCCA‑TCCGGCGCAGCGAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGCCCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTTCGCCTTTTTCACGCCCCGGCGCGCG‑AC‑CAAA‑CAT‑CACCGACACTGGCACCATCTGCACATCCA‑G‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGT‑CGTGGAACAGCTTAATAGACTCTTCTTTCGGCgtg                                                                                                                            <  1:179435/251‑1 (MQ=255)
                                                                                                                                             cgggaaaaC‑GGACGGCGAGAAACGCACAAACCTGTGGCGACCGAGCCACAGTACAGCGAAAAATGCCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTC‑ACTTCGCCTTTTTCACGCCCCGGCGCGCG‑AC‑CAAA‑CAT‑CACCGACACTGGCACCATCTGCACATCCA‑G‑ATTTGGGTTGCGACGGTGCAAATCGAGATAGT‑CGTTGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAacacac                                                                                                            <  2:242288/246‑1 (MQ=255)
                                                                                                                                                          cGGCGCGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTC‑ACTTCGCCTTTTTTACCCCCCGCCGCGCG‑AC‑CCAA‑CAT‑CACCGAACCTGGCCCCCTCTGCACCTCCC‑C‑ATATTGGTTGCTTCGGTGGCCATCGGGATAAT‑CGTGGGCAAACTTAATAGAACCTTCTTTTGCCGTGTAATAGGTGCAAAAACCCGGCccgccgg                                                                                                >  1:184344/1‑250 (MQ=255)
                                                                                                                                                           ggCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTC‑ACTTCGCCTTTTTTACGACCCGGCGCGCG‑AC‑CAAA‑CAA‑CCCCCCCACCGGCCCCATCTGCACATCAA‑G‑AATTGGGTTGCCCAGGAGCAAAACGAGATAGA‑CGGGGTGAAGCTTGATAGAATCTTTTTTCCGGGGGTAATAAGGGGACAAACGCGGGCCGCCGtg                                                                                               >  2:369182/1‑251 (MQ=255)
                                                                                                                                                             cGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTC‑CATTCGCCTTTTTTACGCCCCGGGGCGGG‑GC‑CAAA‑AATACACC‑CCACAGGGACCATCTGAAAACCCA‑G‑AGTTGGGGGGCTCCGGAGCAAAAAGAGATAGT‑CGTGGGAAAGATTAAAAAACTATTCTTTCAGGGTGGAAGAAGAGTACAAACGCGGCccgccgcga                                                                                              >  2:307146/1‑247 (MQ=255)
                                                                                                                                                                gaAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTTCGCCTTTTTCACGCCCCGGCGCGCG‑AC‑CAAA‑CAT‑CACCGACACTGGCACCATCTGCACATCCA‑G‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGT‑CGTGGAACAGCTTAATAGACTCTTCTTTc                                                                                                                                  <  1:254545/211‑1 (MQ=255)
                                                                                                                                                                     gcaaaaaccgtcggggACCGAGCCCCAGTACAGAAAAAAATTTCTGTCCCCTGTTAAGCATCCGCGGCGGCGGGTTC‑TCTTCCCCTTTTCCACCCCCCGCCGCGCG‑AC‑CAAA‑CAT‑CACCGACACTGGCACCATCTGCACATCAA‑G‑ATTGGGGTTGCTACGGTGCAAAGCGAGATATT‑CGTGTAACAGCTTAATAGCCTTTTTTTGCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAAcaca                                                                                     <  1:328231/236‑1 (MQ=255)
                                                                                                                                                                      cacaAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTTTGCCTTTTTTACCCCCCGGCGCGCG‑AC‑CAAA‑CCA‑AACCCACACCGGCCCCATCTGCCCCTCCA‑A‑ATTTGGGTTGGTACGGTGGAAATCGAGAAAGTACG‑GGGACAGCTTAATAGACTCTTTTTTCGGCGGGTAATAGGGGAACACACCCGGCCCGCCGCGGATTAAcaca                                                                                     >  2:476734/1‑250 (MQ=255)
                                                                                                                                                                             cTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTTCGCCTTTTTTACGCCCCCGCGCGCG‑AC‑CAAA‑AAT‑CACCGACACTGGCCCCATCTGCACATCCAAG‑TTTTGGGT‑GCTACGGTGCAAATCGAGATAGT‑CGTGGGACAGCTTAATAGACTCTTCTTTCGGCGTGGAATAAGGGAAAACCCGCGGCCCGCCCGGAATGAACACATAGCGCgg                                                                             >  1:109522/1‑251 (MQ=255)
                                                                                                                                                                               gcgccccccGACCCACAGTACATCAAAAATTTTCGGTACCCTGTGAAGCAACCGCGGCGGCGGGTCC‑CCTCCGCCTTTTACACCCCCCGGCGCGCG‑CC‑CAAA‑CCC‑CCCCGACACTCGCACCATCTGCACATCCA‑G‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGC‑CGTGGAACAGCTTAATAGCCTCTCCTTGCGGCGTGTAATAGGTGACCCCCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCa                                                                           <  2:283581/244‑1 (MQ=255)
                                                                                                                                                                                    gcccGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTTCGCCTTTTTCACGCCCCGGCGCGCG‑AC‑CAAA‑CAT‑CACCGACACTGGCACCATCTGCACATCCA‑G‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGT‑CGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAacac                                                                                                              <  2:134/209‑1 (MQ=255)
                                                                                                                                                                                    gacggacccACAGTACAGCAAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGGGGTTTC‑ACTTCGCTTTTTTCACGCCCCGGCGCGCG‑AC‑CAAA‑CAC‑CCCCGACACTGGCCCCATCTGCACATCCA‑G‑ATTTGGGTTGCTAGGGTGAAAATCGAGATAGT‑CGTGGAACAGCGTAATAGACTCTTCTTTCGGCGTGTAAGAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGc                                                                      <  1:353363/244‑1 (MQ=255)
                                                                                                                                                                                      ccgagatacaGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTA‑ACTTCGCCTTTTTTACCCCCCGGGGC‑CG‑GCACAAA‑AAT‑CACCGACAATGGCGCCATCTCCACCCCCA‑G‑ATTTGGGTTGCTACGGGGCAAATAGAGATAGA‑TGTGGAACAGCTTAATAGAACCTTCTTTCTGCGTGGAGTAGGTGGACACACGCGGCCCGCCGGGAATGAAcacaaaacgaggcgggagggg                                                                    >  1:164694/8‑234 (MQ=255)
                                                                                                                                                                                               aGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTTCGCCTTTTTCACGCCCCGGCGCGCT‑AC‑CAAA‑CAT‑CACCGACACTGGCACCATCTGCACATCCA‑G‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGT‑CGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGa                                                                                                                  <  1:573374/196‑1 (MQ=255)
                                                                                                                                                                                                  aCAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGAGGCGGGATC‑ACTTCGCATTTTTCACGCCCCGGCGCGCG‑GC‑CAAA‑AAT‑CACCGACAAAGGCACCATCTTCACAACCC‑A‑ATTTGGGGTGACACGGGGGAAAACGAGATAGT‑CGAGGGACAGCTTAATAAACTCTTTTTTTGGCGAGGAATAGGTGAAAACACGCGGCACGCCGGGAATGAACACATAGGGCGGCAGTAGAGTGCCATCGagttt                                                        >  1:256981/1‑247 (MQ=255)
                                                                                                                                                                                                               tCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTC‑ACTTCGCCTTTTTTACCCCCCGGCGCGCG‑AC‑CAAA‑CAT‑CACCCACACTGGCACCCTCTGCACATCCA‑G‑ATTTGGGGTGCTACGGGGCAAATCGAGAAAGG‑CGTGGAACAGCGTAATAGACTCTTCTTTCGGCGTGTAATAGGGGAACAAACGCCGCCCGCCCTGAATGGACACATAACGCGGCAGGAGGGGGCCGGCGGTTTCCAACGGcccttac                                           >  1:343651/1‑245 (MQ=255)
                                                                                                                                                                                                                  gtccgccgtaaaccaaccGCAGCGGCGGGTCC‑ACTCCCCCTTTTCCCCCCCCGGGCCCGC‑‑AC‑CAAAACAC‑CCCCGACACTGGCCCCACCTGCAC‑TCCAAG‑ATTTGGGTTGCTACGTTCCAAATCGAGATAGT‑CGTGGAACAGCTTAATAGCCTCTTCTTTCGGCGTGTAATAGGTGAACCCCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACgg                                         <  1:520353/233‑1 (MQ=255)
                                                                                                                                                                                                                                   actcaggggcggtttcattctccttttttCCCCCCCCGGGGCGCC‑AC‑CAAA‑CAT‑CACCGACACTGGCCCTATCTTCACTTCCA‑G‑TTTGGGGTTTCTTCGGGGAAAATCGAGATAGG‑CGTGGAACAGCTTAATAGACTCTTCTGTCGGCGGGAAAGAGGTAAACACTCGCGTCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCAtgt                         <  1:281109/226‑1 (MQ=255)
                                                                                                                                                                                                                                     gcgggggcggttccccTTCGCCTTTTCCCCGCCCCGGCGCGCG‑AC‑CAAA‑CAC‑CCCCGACCCTGGCACCATCTGCACATCCA‑G‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGT‑CGGGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGcc                      <  1:418418/236‑1 (MQ=255)
                                                                                                                                                                                                                                         ggcggGTTC‑ACTTCGCCTTTTTCACGCCCCGGCGCGCG‑AC‑CAAA‑CAT‑CACCGACACTGGCCCCATCTGCACATCCA‑G‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGT‑CGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGGGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCCTGTGCCAGacc                 >  2:164154/1‑250 (MQ=255)
                                                                                                                                                                                                                                                 c‑ACTTCGCCTTTTTCACGCCCCGGCGCGCG‑AC‑CAAA‑CCT‑CACCGACACTGGCACCATCTGCACATCCA‑G‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGT‑CGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGgcgc         >  1:590978/1‑251 (MQ=255)
                                                                                                                                                                                                                                                    cTTCGCCTTTTTCACGCCCCGGCGCGCG‑AC‑CAAA‑CAT‑CACCGACACTGGCACCATCTGCACATCCA‑G‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGT‑CGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGgcgggc       >  2:581152/1‑248 (MQ=255)
                                                                                                                                                                                                                                                       ccccTTTTTCCCCCCCCGCCGCCCG‑CC‑CAAA‑CCC‑CCCCGCCACTGGCCCCATCTGCCCATCCA‑T‑ATTTGGTTTGCTCCGGTGCAAATCGAGATAGT‑CGTGGAACAGCTTAATAGCCCCTTCTTTCGGCGTTTAATAGGTGACCACCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAAc    <  1:307146/249‑1 (MQ=255)
                                                                                                                                                                                                                                                         ccTTTTTCACGCCCCGGCGCGCG‑AC‑CAAA‑CAT‑CACCGACACTGGCACCATCTGCACATCCA‑G‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGT‑CGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACCTAGCGCGGCAGTAGAGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGAGCCAGACACTGGGCGAGCAACGt  >  2:483058/1‑251 (MQ=255)
                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                          
TATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGT‑GCCGTGTT‑C‑ATCCGCCA‑TACGGCGCAGCGAAAC‑TGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTC‑ACTTCGCCTTTTTCACGCCCCGGCGCGCG‑AC‑CAAA‑CAT‑CACCGACACTGGCACCATCTGCACATCCA‑G‑ATTTGGGTTGCTACGGTGCAAATCGAGATAGT‑CGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGT  >  NC_000913/4255812‑4256304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑