Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,342,1010CG21.0% 129.2 / 12.5 62P279R (CCG→CGG) kdsDD‑arabinose 5‑phosphate isomerase KdsD
Reads supporting (aligned to +/- strand):  ref base C (36/13);  new base G (0/13);  total (36/26)
Fisher's exact test for biased strand distribution p-value = 1.25e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATT‑TGCGATGACAATATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATGAGCAAAGCAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACAT  >  NC_000913/3341851‑3342349
                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                           
ggcgCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATT‑TGCGATGACAATATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGcc                                                                                                                                                                                                                                                             >  1:446421/1‑251 (MQ=255)
   gcACTGGGTCGTCCACTTCTGCTGCGCGTAAACGATCTTATGCATACGGGCGATGCGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATT‑TGCGATGCCAATATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCggg                                                                                                                                                                                                                                                          >  2:582330/1‑251 (MQ=255)
   gcACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATT‑TGCGATGACAATATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCggg                                                                                                                                                                                                                                                          <  1:565520/251‑1 (MQ=255)
    cACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATT‑TGCGATGACAATATGCTGCTT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCgggg                                                                                                                                                                                                                                                         >  2:296555/1‑251 (MQ=255)
           gcGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATG‑TGCGATGACAATATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGAGGTGATGACGCCGGGGGGAAT‑Ac                                                                                                                                                                                                                                                 <  1:99623/250‑1 (MQ=255)
                       gctgcGCTTAAACGATATTATGCATGCGGGCGAGGAGATCCAGCACGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATT‑TGCGATGACTATATGATGATT‑GAAGGCATCTTTACCGACGGTGATGTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGATGCCGGGGGGAAT‑ACGTGTGCGCCCTg                                                                                                                                                                                                                                     <  2:394/251‑1 (MQ=255)
                               tAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATT‑TGCGATGACAATATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGCTGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTg                                                                                                                                                                                                                            >  2:287145/1‑251 (MQ=255)
                               tAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATT‑TGCGATGACAATATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTg                                                                                                                                                                                                                            >  2:137862/1‑251 (MQ=255)
                                  aCGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATT‑TGCGATGACAATATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGAGGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGcc                                                                                                                                                                                                                         <  1:582330/251‑1 (MQ=255)
                                    gATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATT‑TGCGATGACAATATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGt                                                                                                                                                                                                                       >  2:148320/1‑251 (MQ=255)
                                                  gggCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATT‑TGCGATGACAATATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACt                                                                                                                                                                                                         >  1:91039/1‑251 (MQ=255)
                                                  gggCGAAGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATT‑TGCGATGACAATATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACt                                                                                                                                                                                                         <  1:177090/251‑1 (MQ=255)
                                                      gATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGATGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATT‑TGCGATGACAATATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAt                                                                                                                                                                                                     >  2:476361/1‑251 (MQ=255)
                                                       aTGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATT‑TGCGATGACAATATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑g                                                                                                                                                                                                   >  2:381558/1‑251 (MQ=255)
                                                            aTCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATT‑TGCGATGACAATATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTc                                                                                                                                                                                              <  1:287145/251‑1 (MQ=255)
                                                             tCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATT‑TGCGATGACAATATGATGATT‑GAAGGCATCTTTACCGACGGGGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGCTGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGCACTTAAT‑GCAGTcc                                                                                                                                                                                             >  2:58288/1‑251 (MQ=255)
                                                              cccGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATT‑TGCGATGACAATATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTcc                                                                                                                                                                                             <  1:143060/250‑1 (MQ=255)
                                                                                    aGTCTGCGTGCCGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATT‑TGCGATGACAATATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGt                                                                                                                                                                      >  2:220839/1‑251 (MQ=255)
                                                                                        tGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATT‑TGCGATGACAATATGCTGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGACGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGTCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCCCCTCCGTGCTGGTTGcc                                                                                                                                                                  >  2:259167/1‑251 (MQ=255)
                                                                                                  gTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATT‑TGCGATGACAATATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGAcc                                                                                                                                                        >  2:17415/1‑251 (MQ=255)
                                                                                                     ggtGGAAGTTACCAAAAAAAATTTTGGTATGACTGTTATT‑TGCTATAACAATATGATTAGT‑GAAGGCATTTTCCCCGACGTTTATTTACGCTGTTGT‑TTCGAT‑GTGGGCGTGG‑ATGTTGTTCATTAAAG‑TATTGCCTTTGTGATGCCGCGGGGGGAAAT‑ACGTGTGCGCCGGGG‑CTTTCGGGCGGTTGGGGCACTGAATTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCAt                                                                                                                                                      <  1:365239/248‑1 (MQ=255)
                                                                                                             ttACCCGCAAAAATCTTGGTATGACTGTCATT‑TGCGATGACAATATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCa                                                                                                                                                                                   >  1:453840/1‑213 (MQ=255)
                                                                                                             ttACCCGCAAAAATCTTGGTATGACTGTCATT‑TGCGATGACAATATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGgt                                                                                                                                             >  2:582632/1‑251 (MQ=255)
                                                                                                               ccccGAAAAAATCTTGTTATGATTGT‑ATTGTGGGATGACAATAGGA‑GATTAGAAGGCTTTTTTACCGAGGGTGATTTACGCGGT‑GTCTGCGAT‑ATGGGCGTGT‑TTGTCCGTCATTAAAT‑TGTGGCCGGGGTGGTGCCGCCGGGGGAAAG‑ACGGGTGCGCCCTGG‑CATTCTGTTCGTTGAGGCACTGATCTTAAT‑GGAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGgtgt                                                                                                                                           <  1:361748/250‑1 (MQ=255)
                                                                                                                   gaaaaaaTTTTGTTATGTCTGTCTTT‑TGCGATGATAAGATGTTGATG‑GAGGGCTTTTTTCCCGTGGGTGATTTACGCGTT‑GTCTTCGAT‑‑GGGGGGTGGGTTGTTCGTCA‑TTAAGGTATTGCCTATGTGATGCCGCGGGGGGGATT‑ATGTGGGC‑CCCTGGGTATTCGGGCTGGTGAGGCTCGGAACTAAAG‑GTAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTa                                                                                                                                       <  2:508439/249‑1 (MQ=255)
                                                                                                                    cAAAAATCTTGGTATGACTGTCATT‑TGCGATGACAATATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTAc                                                                                                                                      >  2:405385/1‑251 (MQ=255)
                                                                                                                    aaaaaaTTTTGGTATGATTTTTATT‑TGGGATGAAAATATGATGTTT‑GAAGGCTTTTTTACCGGCGGTGATTTACGCGGT‑TTCTTCGAT‑GGGGGCGGGG‑TTTTTCTTCATTTAAG‑TATTGCGGATGTGATGACGCGGGGGGGAAT‑TCGTGTGCGCCTTGG‑CTTTCTGGCCGTGGAGGCACTGATTTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTAc                                                                                                                                      <  1:458257/250‑1 (MQ=255)
                                                                                                                     aaaatttttGGTATGACTGTCATT‑GGCGATGAAAATAGGATGATT‑GAAGGCATCTTTCCCGACGGTGATTTCCGCCGT‑GTTTTCGAT‑AGGGGCGGGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGCCGCGGGGGGGAAT‑AGGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGACCTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACa                                                                                                                                     <  1:17415/244‑1 (MQ=255)
                                                                                                                          tCTTGGTATGACTTTCATT‑TGCGATGACAATAGGATGATT‑GAAGGCATTTTTGCCGGCGGTTTTTTCCGCCGT‑GTTTTAGAT‑GTGGGCGTGT‑TTGTTCGTCATTTAAG‑TATTGCCGATGTTTTGACGCCGGGGGGAAT‑CCGTGTGCGCCGTGG‑TAGTCTGGCCGTTGGGCCTCTGAATTTAAT‑GCGGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATg                                                                                                                                 <  1:480591/250‑1 (MQ=255)
                                                                                                                             tgttATGACTGTTATT‑TGCGATGACAATATGATGATT‑GAAGGCATTTTGCCCGAGGGTGATTTACGCGGT‑GTCTTCGAT‑AGGGGGGTGG‑ATGTTCGTATGTTAAG‑TATTGCCGATGGGATGACGCCGGGGGGAAT‑ACGTGGGCGCCCTGG‑CATTCTGGCCGTGGAGGCACTGATCTTATT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATg                                                                                                                                 <  1:183627/244‑1 (MQ=255)
                                                                                                                                   gACTGTCATT‑TGCGATGACAATATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTAc                                                                                                                       >  1:120620/1‑251 (MQ=255)
                                                                                                                                       gTCATT‑TGCGATCACAATATGATGCTT‑GAAGGCGTCATTACCGACGGTGATTTAAGCCGT‑CTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTCAAG‑TATTGACGATGTGATGACGCCGGGGGGAAT‑CCGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGACACTGAATTTAAT‑GAAGACCCGCCATCTCACATCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCg                                                                                                                   >  2:502067/1‑251 (MQ=255)
                                                                                                                                        tCATT‑TGCGATGACAATATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGt                                                                                                                  >  2:7148/1‑251 (MQ=255)
                                                                                                                                          tattgtcgaagaaaaTATGAGGATT‑GAAGGATTTTTTGCCGCGGGTGTTTTCCGCGGT‑GTTTGCGAT‑AGGGGCGGGG‑ATGTTCGACAGTTAAG‑TATTCCGGTTGGAATGACGCGGGGGGGAAT‑TCTGGTGCCCCGTGG‑CTTTCTGGCGGTGGGGGCTCAGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGt                                                                                                                  <  2:158797/237‑1 (MQ=255)
                                                                                                                                            tgtGCGATTAAAATATGATGATT‑GAAGGCATCTTTACCGAAGGTGATTTACGCCGA‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGGAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGGTGGTTGACGTTAGCGACCATTTTCTCGGTGTGTTACATATGCATGATTTAATGCGTGc                                                                                                                >  2:234199/3‑250 (MQ=255)
                                                                                                                                               gCGATGACAATATGATGATT‑GAAGGCATCTTTACAGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGc                                                                                                            <  1:438428/251‑1 (MQ=255)
                                                                                                                                               gCGAAGACAATAAGTTGAAT‑GAGGGCATTTTTACCGGCGGGGTTTTCCGCCGT‑GTCTTCGAT‑AGGGGGGGGG‑ATTTTCGTGAGTTAAG‑TGTTGCCGATGTGAGGACGCGGGGGGGAAG‑ACGTGGCCGCCCTGG‑CATTCTGGCCGTGGGGCCAGTGAACTAAAT‑GCATTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGc                                                                                                            <  1:476361/251‑1 (MQ=255)
                                                                                                                                                  aTGACAATATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGCGt                                                                                                          >  2:144051/1‑250 (MQ=255)
                                                                                                                                                       aaTATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGGTGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGGTTTACTGCGTGCAGGCGTAgtgt                                                                                                     >  2:91153/1‑250 (MQ=255)
                                                                                                                                                       aaTATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAgtgt                                                                                                     >  1:94501/1‑250 (MQ=255)
                                                                                                                                                         catgatgATT‑GAAGGCATCTTTACCGACGGAGATTTACGCCGT‑GTCTTAGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGGGATGACGACGGGGGGAAT‑ACGTGTGAGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCTGTCCCGCCCTATCACCTCAGTGATGGTTGCCGATGGCGACCATTTACTCGGTGCGTTCCATATGCATGATTTCCTGAGTGCAGGCGGAgtggaa                                                                                                   >  2:458059/2‑247 (MQ=255)
                                                                                                                                                              tgatT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCGCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTc                                                                                                                                                                                              <  1:338171/154‑1 (MQ=255)
                                                                                                                                                              tgatT‑GAAGGCAACTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTc                                                                                                                                                                                              >  2:338171/1‑154 (MQ=255)
                                                                                                                                                                 tt‑GAAGGCATCTTTACCGAAGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGAGATGACGCCGGGGGGAAT‑ACGTGTGCGACCTGG‑CATTGTGGCCGTTGAGGCACTGAACTTAAT‑GCAGGCCCGCCATATCACCTCAGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGAAGGCGTAGTGGAAGGATTCa                                                                                            >  2:524489/1‑249 (MQ=255)
                                                                                                                                                                        gCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGCAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATaa                                                                                     >  2:457409/1‑250 (MQ=255)
                                                                                                                                                                             tttACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAaacaac                                                                                >  2:590428/1‑250 (MQ=255)
                                                                                                                                                                                aCCGACGGGGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATg                                                                            >  1:60541/1‑251 (MQ=255)
                                                                                                                                                                                 ccgacggatgtttACCCCGT‑GTCTTCGAT‑GTGGGCGGGG‑GTTGGCGTCAGTGATG‑TATTGCGGATGTGATGCCGCGGGGGGGAAT‑ATTTGTGCCCCCTGA‑CTTTCGGGCCGTGGAGGCTTTGAACTTAAG‑GCATTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATGa                                                                           <  1:494028/241‑1 (MQ=255)
                                                                                                                                                                                 ccGACGGTGATTTCCGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATg                                                                            >  2:127653/1‑250 (MQ=255)
                                                                                                                                                                                 ccGACGGTGATTTACGCCGT‑GT‑TTTGATTATGGGCGTGG‑ATTTGCGTCATTTAAG‑TATTGCCTATGTGAGGCCGCGGGGGGGAAT‑ACGTGGGCGCCTTGG‑CTTTCGGGCCGTGGGGGAACTGAA‑TTAATTGCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATGa                                                                           <  1:394927/251‑1 (MQ=255)
                                                                                                                                                                                   gACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATGAGc                                                                         >  2:324914/1‑251 (MQ=255)
                                                                                                                                                                                                       tctgcGAT‑AGGGGCGTGG‑TTGTTCTCCAGTTAAG‑TATGGCGGATGTGATGCCGCGGGGGGGAAT‑ACGTGTGCGCCCTGG‑CTTTCGGGCGGTTGAGGCAATGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATGAGCAAAGCAGGTGCGTCGCtt                                                       <  1:137862/246‑1 (MQ=255)
                                                                                                                                                                                                         ttCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATGAGCAAAGCAGGTGCGTCGCTTGCg                                                    >  2:150013/1‑251 (MQ=255)
                                                                                                                                                                                                              atgtGGGGGTGG‑ATTTCCGTCAGTTAAG‑TATTGCGGATGTGACGACGCGGGGGGGAAT‑ACGTGTGCGCCCTGG‑TAGTCTGGCCGTGGGGGCACTGAACTTAAG‑GCTGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATGAGCAAAGCAGGTGCGTCGCTTGCGACCt                                                <  1:524489/248‑1 (MQ=255)
                                                                                                                                                                                                                gggggCGTGT‑ATTTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCGGGGGGGAAT‑ACGTGTGCCCCCTGG‑CTTTTTGGCCGTTGAGGCATTAAACTAAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATGAGCAAAGCAGGTGCGTCGCTTGCGACCTGt                                              <  1:516295/249‑1 (MQ=255)
                                                                                                                                                                                                                     cGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATGAGCAAAGCAGGTGCGTCGCTTGCGACCTGTTACgg                                         >  1:104578/1‑251 (MQ=255)
                                                                                                                                                                                                                      gggggttgttattCAGTAAAG‑TATTGCGGTGGTGATGACGCCGGGGGAAAA‑CCGTGTGCGCCCTGG‑CTTTCGGGCCGTTGAGGCATTGAATTTAAT‑GAAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATGAGCAAAGCAGGTGCGTCGCTTGCGACCTGTTACgg                                         <  1:381558/239‑1 (MQ=255)
                                                                                                                                                                                                                       tGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATGAGCAAAGCAGGTGCGTCGCTTGCGACCTGTTACGGcc                                       >  1:73083/1‑251 (MQ=255)
                                                                                                                                                                                                                       tGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATGAGCAAAGCAGGTGCGTCGCTTGCGACCTGTTACGGcc                                       >  1:8054/1‑251 (MQ=255)
                                                                                                                                                                                                                                   cAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAAt                                                                             >  1:179214/1‑202 (MQ=255)
                                                                                                                                                                                                                                             tttgcgtttgtgATGGCGCGGGGGGGAAT‑TGGTGTGCGCCCGGG‑CTTTCTGGCGGGGGGGGCACGAAACTTAAT‑GCGGCCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATGAGCAAAGCAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTtat                    <  1:65644/242‑1 (MQ=255)
                                                                                                                                                                                                                                               ggCCGATGTGATGCCGCGGGGGGGAAT‑ACGTGGGCGCCCGGG‑CTTTCGGGCCGTTGAGGCACTGAACTAAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATGAGCAAAGCAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAg                  <  1:324914/249‑1 (MQ=255)
                                                                                                                                                                                                                                                         gatgccgcGGGGGGAATTGCGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGCCACTGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATGAGCAAAGCAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCaga        <  1:590428/244‑1 (MQ=255)
                                                                                                                                                                                                                                                              gCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATGAGCAAAGCAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACAt  >  2:10859/1‑251 (MQ=255)
                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                           
GGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATT‑TGCGATGACAATATGATGATT‑GAAGGCATCTTTACCGACGGTGATTTACGCCGT‑GTCTTCGAT‑ATGGGCGTGG‑ATGTTCGTCAGTTAAG‑TATTGCCGATGTGATGACGCCGGGGGGAAT‑ACGTGTGCGCCCTGG‑CATTCTGGCCGTTGAGGCACTGAACTTAAT‑GCAGTCCCGCCATATCACCTCCGTGATGGTTGCCGATGGCGACCATTTACTCGGTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATGAGCAAAGCAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACAT  >  NC_000913/3341851‑3342349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑