| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 827,507 | 0 | T | G | 29.3% | 81.4 / 16.6 | 60 | D492A (GAC→GCC) | ybhF | ABC exporter ATP binding subunit YbhF |
| Reads supporting (aligned to +/- strand): ref base T (7/34); new base G (16/1); total (24/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.39e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGATCAACTGAATAAAGGCTTGCTCCATGGTGGGATCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT‑GGCAGTTCA‑T‑CGGTGGCGTGGGAGGCGATACTTTT‑CAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCC‑GACCGCGTAAGCCATACACACCA‑GAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGC > NC_000913/827276‑827746 | gggATCAACTGAATAAAGGCTTGCTCCATGGTGGGATCGGGTTGCTCATCGTGAGCCGCCTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATGTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGGGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTtc < 2:441819/250‑1 (MQ=255) tccgttctttAAATGCTTGCTCCATGGTGGAAGCGGGTTGCTCATCGTTACCCGACTGTTCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCGGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTGCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGCGGTCAACGCCGGAAGTCGGTtcgtc < 2:469963/241‑1 (MQ=255) tCAACTGAATAAAGGCTTGCTCCATGGTGGGATCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGGGGGGGGGAACGCCCGAACACGGTtcgtcg > 2:402526/1‑251 (MQ=255) cAACTGAATAAAGGCTTGCTCCATGGTGGGATCGGGTTGTTCATCGTTAGCCGACTGTGTTTGCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGGGGCGGCTGTCTCGACCATGCTGTTGATGTGCAGTCAAAATTCACGGCGGGTGAGGGGGTCAGCGCCGGAAGTTGGGtcgtcg < 2:567909/250‑1 (MQ=255) aaCTGAATAAAGGCTTGCTCCATGGTGGGATCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGGGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCga‑g < 1:35354/251‑1 (MQ=255) gAATAAAGGCTTGCTCCATGGTGGGATCGGGATGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATCTTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAcggcgggaggcggctcggcgggaaa > 1:544435/1‑231 (MQ=255) aTAAAGGCTTGCTCCATGGTGGGATCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGTCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAACTCACGGCGGGGGAGGGGGGCCACGCCGGagggtggtgcgtgcaaagggg > 1:469963/1‑230 (MQ=255) aGGCTTGATCCATGGTGGGATCGGGTTGCTACTCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGGGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGACGGAAGGCGGGTTGTCGA‑GAAACAGAATg > 1:467061/1‑251 (MQ=255) ttGCTCCATGGTGGGATCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGTAACACCCGGAGTAGGTTTGTTGA‑GGAAAAGAATGTCCg > 1:530555/1‑251 (MQ=255) cATGGTGGGATCGGGTTGCTCATCGGTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCAt < 2:368122/251‑1 (MQ=255) aTGGTGGGATCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTACGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGCGGGGGTCAACGCCGGAAGTCGGGTCGTTGA‑GATACAAAAAGTCCgggtacgg > 1:433401/1‑245 (MQ=255) ggtggGATCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGc < 1:432562/250‑1 (MQ=255) tggGTTCGGGTTGCTCATCGTTAGCCGACTGGGCTTTCAAAGCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTGCACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCTA‑GAAACAGAATGTCCGG‑TTCATGCAt < 2:471329/250‑1 (MQ=255) ggATCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGGGGGGGGCAAAGCCCGACGTCGGTTCGGCGA‑GAAACAAAATATCCGG‑tggatcgggagg > 1:552066/1‑240 (MQ=255) ggATCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCAGGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑t < 2:552066/240‑1 (MQ=255) caaTCGTTAGCCGCCTGGGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGTTGCGTTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTTACCATCACCGTGACGCCTTTCTCTACCAGGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACAGgccac < 2:305951/249‑2 (MQ=255) aTCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGTAACTCCCGAAGGCGGTTCGTCGACGAAACA‑AAAGTCCCG‑GTCATGCATCAGCCGACAGgccca > 1:119499/1‑247 (MQ=255) tCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGTCGGGTCGTCGA‑GAAACAGAATGTCCGG‑GTCATGCATCAGCGAACAGgccagcgc > 1:562532/1‑251 (MQ=255) tAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACATgccagcgccag < 2:344233/250‑1 (MQ=255) gCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGCGAGGGGGGCAACGCCCGGAGGCGGTTCGTCGA‑GGAACAGAATGTCCGG‑GTCCTGCATCCGCGAAAAGgccagcccccgccc > 1:506886/1‑243 (MQ=255) aCTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGt < 1:402526/251‑1 (MQ=255) tgtgCTCTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGGAACGCCGGGAGTCGGGTCGTCGG‑GGAAAAGAATGTCCGG‑GTCATGCATCCGCGAACAGGCCAGCGCCCGCCGCTGttt > 1:155411/1‑251 (MQ=255) ctggctgtCAAATCGTCCGGCGTGCTGCTGGCTATTAATTTCCCGCGGGACCCCAGGCCGATGCGGGCGCAATATTCCGCTTCATCCATAAAGGGGGTGGTGACCATCCCCGTGATGCCTTTCGCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAATAGGTCAGCGCCAGCCGCTGt < 2:504092/242‑1 (MQ=255) gtgCTTTCAAATCGACCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGTTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TGGATGCATCAGCTAACAGGCCAGCGCCAGCCGCTGTTTa < 1:89764/251‑1 (MQ=255) tgCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATCTTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGTCAACGCCGGGAGTCGGGTTGTAGA‑GAAACCGAAAGTCCGG‑TTTATGCATCCGCGAACAGGCCAGCGCCCGCCCCCGTTTa > 1:580875/1‑250 (MQ=255) ggCTTGCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCTCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTGTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGTGCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATTTCCGG‑TTCATGCAGCAGGGAACAGTCCAGCGCCAGCCGCTGTTTa < 2:580875/249‑1 (MQ=255) cGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT‑GGCAGtt < 1:547433/251‑1 (MQ=255) gCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT‑GGCAGTTCA‑T‑CGGTgg < 1:117614/251‑1 (MQ=255) gATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAAAATGTCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT‑GGCCGGTCA‑T‑CGGTGGCGgggg > 1:99652/1‑251 (MQ=255) gTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGTCAACGCCGGAAATCGGTTCGTCGA‑GAAACAGAATGTCCGG‑GTCATACATCAGCGCACAGGCCAGCGCCAGCCCCTGGTTAAAAACTAAA‑GGCAGTTCACT‑CGG‑GGCGTGGGGGGCGAGACATTT‑c > 1:388669/1‑250 (MQ=255) ggCCGATGCGGTCGCAATATGCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAAGTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT‑GGCAGTTCA‑T‑CGGTGGCGTGGGAGGCGATACTTTT‑CAGGCCGAAc < 2:464956/251‑1 (MQ=255) gATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAGCCTAAT‑GGCAGTTCA‑T‑CGGTGGCGTGGGAGGCGATTCTTTT‑CAGGCCGATCGCCt < 2:593407/251‑1 (MQ=255) gCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGGGGGGGGCCAAGCCGGAAGTCGGGTCCTCGA‑GAAACAGAAAGTCCGG‑GTCATGCCTCAGCGAAAAAGCCAGCGCCAGCCGGTGTTTAAAAACCAATAGCCAGT‑CA‑TACGG‑GGCCGGGGAGGCGGTACTTTT‑TATGCCGAACGCCCCGCTCaacc > 2:203347/1‑248 (MQ=255) tCCGCTTCATCCATAAAGTGGGTGGTGACCATCTCCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT‑GGCAGTTCA‑T‑CGGTGGCGTGGGAGGCGATACTTTT‑CAGGCCGAACGCCTCGCTCATGCGGgaga < 1:337792/250‑1 (MQ=255) ccGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT‑GGCAGTTCA‑T‑CGGTGGCGTGGGAGGCGATACTTTT‑CAGGCCGAACGCCTCGCTCATGCGGGAGAt < 1:180990/250‑1 (MQ=255) gCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGATGGGGGGGTCAACGCCGGGAGTGGCTTTTTCCG‑GAAACAGAATGTCCGG‑TTCCTGCATCAGCGAAAAGGCCAACGCCAGCCGCCGTTTAAAACCTAAT‑GGCCGGTTA‑T‑TGGGGGCGGGGGAAGGGGGACTTTT‑CAAGCCGCACGCCTCCCCCATGCGGGGGAtttt > 1:573593/1‑251 (MQ=255) cATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT‑GGCAGTTCA‑T‑CGGTGGCGTGGGAGGCGATACTTTT‑CAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCg < 1:10429/251‑1 (MQ=255) attcATAAATTGGGGGGTGACCAGCACCGTGACGCCTGTCTCTACCATTCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGTGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TGCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT‑GGCAGTTCA‑T‑CGGTGGCGTGGGAGGCGAGACTGGT‑CAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGt < 1:46323/248‑1 (MQ=255) aTCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGCGGGGGGCAACGCCGGAAAGCGGGGCGGCGA‑GAAACAGAAAGGCCGG‑GTCATGCATCAGCGGACAAGCCAACGCCCCCCCCTGGTTTAAACCCAAT‑GGCAGGTCA‑T‑CGGTGGGGGGGGGGGCGCTAATTTT‑TATGCCGGAAGCCTCGCTCATGCGGGAGGtttttttgt > 1:305466/1‑248 (MQ=255) ggtggtGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT‑GGCAGTTCA‑T‑CGGTGGCGTGGGAGGCGATACTTTT‑CAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCC‑GAc < 1:304020/251‑1 (MQ=255) gtgACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT‑GGCAGTTCA‑T‑CGGTGGCGTGGGAGGCGATACTTTT‑CAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCC‑GACcgcg < 2:254228/251‑1 (MQ=255) aTCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT‑GGCAGTTCA‑T‑CGGTGGCGTGGGAGGCGATACTTTT‑CAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCC‑GACCGCGTAAGcc < 1:538875/251‑1 (MQ=255) tCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGGGGGGGGCAACGCCGGAAATCGGGTCGTCGG‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGGACAGGCCAGCGCCCGCCGCCGGTTAAAACCCAAT‑TGCAGTTTC‑T‑CGGTGGCGTGGGGGGCGGTACTTTT‑CAGGACGAAACCCCCGCTCACGCGGGGGATTTTTTTGTTTTGCGCCC‑GCCCGCGTaaaccc > 1:156080/1‑247 (MQ=255) cGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT‑GGCAGTTCA‑T‑CGGTGGCGTGGGAGGCGATACTTTT‑CAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCC‑GACCGCGTAAGCCATaca < 1:270696/251‑1 (MQ=255) gACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT‑GGCAGTTCA‑T‑CGGTGGCGTGGGAGGCGATACTTTT‑CAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCC‑GACCGCGTAAGCCATacaca < 2:562532/250‑1 (MQ=255) cTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAAAGCCCGGAGACGGGTCGTCGA‑GAAACAGAAAGGCCGGGTTC‑TGCATCAGCGAAAAAGACAACGCCCGCCGCTGTTTAAAAACTCAA‑GGCGGATTA‑T‑CGGGGGGGGGGGGGGGGAGACTTTTTCAGGC‑GGACGCCTCCCTCCAGCGGGGGGGTTTTTTGTTCTGCGCCC‑CACCCCGTAAGCCCTacaccacag > 1:307037/1‑244 (MQ=255) ttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT‑GGCAGTTCA‑T‑CGGTGGCGTGGGAGGCGATACTTTT‑CAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCC‑GACCGCGTAAGCCATACACACCA‑gaga < 2:155411/250‑1 (MQ=255) aCCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT‑GTCAGTTCA‑T‑CGGTGGCg < 2:348212/153‑1 (MQ=255) aCCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGACGGCGGGGATAACGACGGCCGTCGGTTCGTCAA‑GAAAACGAATTTCCGG‑TTACTACATCCACGAACACGCACCCGACAGCCGCTGGTTAAAAACCAAT‑CGAAGTTTA‑T‑AGGAGGGGGGGGGGGCGATACTTTT‑TAAGCCCAACCCCTCGCTCCTGAGGGGGGGTTTTTTGTTCTGCGCCC‑GACCCCGTAAGCCATACACACCC‑CAGaaaaaacg > 1:588673/1‑248 (MQ=255) aTGCTGTTGATGTGCAGCCATAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT‑GGCAGTTCA‑T‑CGGTGGCGTGGGAGGCGATACTTTT‑CAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCC‑GACCGCGTAAGCCATACACACCA‑GAGAAAAAGCGTaa < 2:530555/251‑1 (MQ=255) tGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGGCGGGTCGTAGA‑GAAACAGAATGTACGG‑GTTATTCCTCAGCGAACAGGGCCGCGCCAGCCGCTGTTTTAAACCTAAT‑GGGCGTTCA‑C‑CGGGGGCGGGGGGGGGGGGACTTTT‑TAGGCCGGACCCCCCGCCCCTGCGGGGGGGTTTTTTGTTCTGCGCCCCGCCCG‑GTAAACCCTACACACCC‑GAGaaaaaaagtaaaatt > 2:31171/1‑241 (MQ=255) ggATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT‑GGCAGTTCA‑T‑CGGTGGCGTGGGAGGCGATACTTTT‑CAGGCCGAACGCCGCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCC‑GACCGCGTAAGCCATACACACCA‑GAGAAAAAGCGTAAATTCTg < 2:359987/249‑1 (MQ=255) ccAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT‑GGCAGTTCA‑T‑CGGTGGCGTGGGAGGCGATACTTTT‑CAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCC‑GACCGCGTAAGCCATACACACCA‑GAGAAAAAGCGTAAATTCTGTTCGACCGTCa < 1:21419/251‑1 (MQ=255) aaaaTTCACGGGGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGCCGA‑GAACCAGAATGTCCGG‑TTCATGAAGCAGCGAACAGGCCAGCGCCAGCCGCTGGTTAAAACCTAAT‑GGCAGTTCA‑T‑CGGTGGCGTGGGAGGCGATACTTTT‑CAGGCCGAACGCCTCGCTCATGCGGTATATTTTTTCGGTCTGCGCCC‑GACCGCGTAAGCCATACACACCA‑GAGAATAAGCGTAAATTCTGTTCGACCTTCAgg < 2:374571/251‑1 (MQ=255) cACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGGTCGTCGA‑GAAACAGAATGCCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT‑GGCAGTTCA‑T‑CGGTGGCGTGGGAGGCGATACTTTT‑CAGTCCGAACGCCTCGCTCATGCGGGAGATTTTGTCGTTCTGCGCCC‑GACCGCTTAAGCCATACACACCA‑GAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTAcc < 2:527442/250‑1 (MQ=255) gggTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT‑GGCAGTTCA‑T‑CGGTGGCGTGGGAGGCGATACTTTT‑CAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCC‑GACCGCGTAAGCCATACACACCA‑GAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGc > 1:2874/1‑251 (MQ=255) gAGGGGGTCAACGACGGCAGTCGGTTAGTCGA‑GAAACCGAATGTCCGG‑TTCATGCATCCGCGCACAAGCCAGCGCCAGACGCTGTTTAAAACCTAAT‑GGCAGTTAC‑T‑CGGTGGTGTGGGCGGCGAAACTTTT‑CAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCC‑GACCGCGTAAGCCATACAC‑CCACGAGAAAAAGCGtcacatatgtt > 2:552487/1‑214 (MQ=255) gAGGGGGCCAACGCCGTTAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT‑GGCAGTTCA‑T‑CGGGGGCGTGGGAGGCGATACTTTT‑CAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCC‑GACCGCTTAAGCCGAACACACCA‑GAGAAAAAGCGTAAATTCTGtt < 1:552487/224‑1 (MQ=255) aGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT‑GGCAGTTCA‑T‑CGGTGGCGTGGGAGGCGATACTTTT‑CAGGCCGAACGCCTCGCTCATGCGGGAGAttt < 2:405717/163‑1 (MQ=255) aGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT‑GGCAGTTCA‑T‑CGGTGGCGTGGGAGGCGATACTTTT‑CAGGCCGAACGCCTCGCTCATGCGGGAGAttt > 1:405717/1‑163 (MQ=255) | TGGATCAACTGAATAAAGGCTTGCTCCATGGTGGGATCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGA‑GAAACAGAATGTCCGG‑TTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT‑GGCAGTTCA‑T‑CGGTGGCGTGGGAGGCGATACTTTT‑CAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCC‑GACCGCGTAAGCCATACACACCA‑GAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGC > NC_000913/827276‑827746 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |