Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,111,727 2 bp→TT coding (865‑866/2544 nt) opgH → osmoregulated periplasmic glucans biosynthesis protein H

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,111,7270CT100.0% 86.7 / NA 26P289M (CCG→TTG) opgHosmoregulated periplasmic glucans biosynthesis protein H
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/8);  total (18/8)
*NC_0009131,111,7280CT100.0% 85.5 / NA 26P289M (CCG→TTG) opgHosmoregulated periplasmic glucans biosynthesis protein H
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/8);  total (18/8)

GATTCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGC  >  NC_000913/1111485‑1111956
                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                    
gATTCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATc                                                                                                                                                                                                                               <  1:173017/251‑1 (MQ=255)
      ggACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTc                                                                                                                                                                                                                         <  2:3011/251‑1 (MQ=255)
             gTTAATCGGCTTCCTCCAACTGCTTATTGGCCGCGATAAATACAGTATATCTGCGTCAACAGTAGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAACAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGc                                                                                                                                                                                                                  >  2:294895/1‑251 (MQ=255)
                 aTGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCaaa                                                                                                                                                                                                               >  1:6531/1‑250 (MQ=255)
                                cTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCt                                                                                                                                                                                               >  2:34292/1‑251 (MQ=255)
                                                    aTACAGTATATCTGCGTCAAAAGTCGGCGATGAACCATTACACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGACGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCCGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGACGGCGGTGGCg                                                                                                                                                                           >  2:143874/1‑251 (MQ=255)
                                                      aCAGTATATCTGCGTCAACAGTTGGCGATGAACCATTACACCCGGAGCATCGCACGGAGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGa                                                                                                                                                                          >  1:144050/1‑250 (MQ=255)
                                                                  cGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTc                                                                                                                                                             >  2:15776/1‑251 (MQ=255)
                                                                                    aaCCATTAAACCCGGAGCATAGCACGGCGTTGATCATGCCTATCTGTAACAAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAACAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTcgc                                                                                                                                           >  2:29564/1‑251 (MQ=255)
                                                                                               ccGGAGCATCGCCCGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCg                                                                                                                                <  2:55957/251‑1 (MQ=255)
                                                                                                       tCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCg                                                                                                                        >  1:35733/1‑251 (MQ=255)
                                                                                                               cGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTACAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCATGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATc                                                                                                                >  1:48700/1‑251 (MQ=255)
                                                                                                                  tGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCTCCGTCGCCGCGTGAAGCGTAAAATCGGTAATATCGa                                                                                                              <  1:243430/250‑1 (MQ=255)
                                                                                                                             atttGTAACGAAGACGTGAACCGTGTTTTTGCCGGACTGCGTGCAACGGGGGAATCAGTAAAAGCCAACGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTAGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTTAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCg                                                                                                  <  2:236024/248‑1 (MQ=255)
                                                                                                                                      gAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTgggg                                                                                         >  1:11729/1‑251 (MQ=255)
                                                                                                                                         gACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGc                                                                                        >  2:180128/1‑249 (MQ=255)
                                                                                                                                           cGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCCGTAAAAGCCACCGGGAATGCCAACCACTTTGATGTCTACATTCTTAGTGACAGTTCTAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGCTTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATAGATGACTTCTGCCGTCGCTGGGGCAGcc                                                                                    >  1:307336/1‑251 (MQ=255)
                                                                                                                                                 ccGTGTTTTTGCTGGACTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGACCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTATAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACa                                                                              <  2:224804/251‑1 (MQ=255)
                                                                                                                                                         ttGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACAtg                                                                      >  2:59406/1‑251 (MQ=255)
                                                                                                                                                                           cGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACAATTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGAc                                                    >  1:41469/1‑251 (MQ=255)
                                                                                                                                                                            gTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACt                                                   >  1:95657/1‑251 (MQ=255)
                                                                                                                                                                            gTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCGACATGGTGGTGCTGGATGCTGACt                                                   <  2:95657/251‑1 (MQ=255)
                                                                                                                                                                                 aatccttcccctccACCGGGACTGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGCGCAAAACGCCTGGATTGAGCTTATCGCTGAAGTCGGTGGCGAATGTCAGATTTTCTATCGCCGCCTCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGCCGCTGGGGCAGCCCGTACAGCTACATGGCGGTGCTGTATGCTGACTCGGt                                               >  1:74345/13‑250 (MQ=255)
                                                                                                                                                                                                   ggAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTAGCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGAttgtttg                            <  2:298663/251‑1 (MQ=255)
                                                                                                                                                                                                            aaCACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTg                   >  2:1826/1‑251 (MQ=255)
                                                                                                                                                                                                                             aTTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGGGCGCCTGATGGAAGc  >  2:121434/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                    
GATTCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGC  >  NC_000913/1111485‑1111956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑