Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	69,014	A→G	F345F (TTT→TTC)	araB ←	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	69,014	0	A	G	100.0%	92.3 / NA	28	F345F (TTT→TTC)	araB	ribulokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/13);  total (15/13)

AGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTCGGTGTGCGGCGGCCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCA  >  NC_000913/68799‑69260                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       aGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGcc                                                                                                                                                                                                                     >  1:117777/1‑251 (MQ=255)  gATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCt                                                                                                                                                                                                                    <  2:65788/251‑1 (MQ=255)  gATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGGCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGTCGTAGATATCACCAAACGCCGATTGGCCt                                                                                                                                                                                                                    >  2:118187/1‑251 (MQ=255)         aaTCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCa                                                                                                                                                                                                             <  1:128497/251‑1 (MQ=255)                     cAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAAt                                                                                                                                                                                                  <  1:27121/250‑1 (MQ=255)                          gTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGACGAGCACAACCGGCAGGAGATCCAGCGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAACAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGCGTACGCGAACGAACCAGGCGTAGATATCACCAAACGCCGCTTGGCCTGCTTCCAGACCGACAAATCCAGGc                                                                                                                                                                                            >  1:39172/1‑251 (MQ=255)                                           gagcgccgaccGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCCAGCTGTTCCAGCGGCCAGCTGAGGACGCGACCGCACCAGGCGTAGATATCACCAAACGCCGATTGGACAGCTTCCAGACCGATACATCCAGGCACCCCCCTGCCATCAAc                                                                                                                                                                           >  2:271667/10‑251 (MQ=255)                                              cgacgaccGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTg                                                                                                                                                                        <  1:318852/245‑1 (MQ=255)                                                 cgaccGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGCTTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCCGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGAcc                                                                                                                                                                     >  2:131143/4‑251 (MQ=255)                                                  gaccGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCg                                                                                                                                                                    <  2:117777/249‑1 (MQ=255)                                                         aaaCCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATAc                                                                                                                                                             <  2:265826/251‑1 (MQ=255)                                                                             gCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCa                                                                                                                                         <  1:295499/251‑1 (MQ=255)                                                                                        agagACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCACGCTCGCCAACGCTCTGttt                                                                                                                              >  1:254249/1‑251 (MQ=255)                                                                                                                     cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGaa                                                                                                  <  2:146259/250‑1 (MQ=255)                                                                                                                      aGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGCTTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGacgt                                                                                                >  2:243390/1‑248 (MQ=255)                                                                                                                      aGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGt                                                                                                >  1:52357/1‑251 (MQ=255)                                                                                                                               aGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTCCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAAc                                                                                       <  1:8260/251‑1 (MQ=255)                                                                                                                                   gttgttTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGCTGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAACTACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACtttt                                                                                   >  2:305819/1‑251 (MQ=255)                                                                                                                                            ggctggcGGTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGc                                                                           <  1:235316/250‑1 (MQ=255)                                                                                                                                                                    gTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACt                                                  >  2:78032/1‑251 (MQ=255)                                                                                                                                                                              cTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCatat                                        >  1:26001/1‑251 (MQ=255)                                                                                                                                                                               tGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACTGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATg                                       >  2:222163/1‑251 (MQ=255)                                                                                                                                                                                    ggcAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGt                                  <  2:83145/251‑1 (MQ=255)                                                                                                                                                                                                   gggCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGGCGCCgg                   <  2:52357/250‑1 (MQ=255)                                                                                                                                                                                                          ctgAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCTAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATcac            >  2:224235/3‑251 (MQ=255)                                                                                                                                                                                                           tgAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATcacc           >  1:218858/2‑251 (MQ=255)                                                                                                                                                                                                             aGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATcacccc         >  2:261349/1‑249 (MQ=255)                                                                                                                                                                                                                   cgACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCGGGTGGAAGTACCGA‑AACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTca  <  1:162309/251‑1 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       AGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTCGGTGTGCGGCGGCCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGGAAATCACCACGCTTTCA  >  NC_000913/68799‑69260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑