Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	69,001	C→T	G350S (GGC→AGC)	araB ←	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	69,001	0	C	T	100.0%	85.2 / NA	25	G350S (GGC→AGC)	araB	ribulokinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/13);  total (12/13)

AATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTCGGTGTGCGGCGGCCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGG  >  NC_000913/68756‑69243                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   aaTCAACCCGCCGAACAGCAGCGGGGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTa                                                                                                                                                                                                                                               <  2:69623/251‑1 (MQ=255)                                            aGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGcc                                                                                                                                                                                                    >  1:117777/1‑251 (MQ=255)                                             gATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCt                                                                                                                                                                                                   <  2:65788/251‑1 (MQ=255)                                             gATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGGCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGTCGTAGATATCACCAAACGCCGATTGGCCt                                                                                                                                                                                                   >  2:118187/1‑251 (MQ=255)                                                    aaTCACCCCTTTCAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCa                                                                                                                                                                                            <  1:128497/251‑1 (MQ=255)                                                                cAGGCGTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAAt                                                                                                                                                                                 <  1:27121/250‑1 (MQ=255)                                                                     gTTGGTTAGCGTTTGGCGAGCGACGACCGTTAAACCAGACGAGCACAACCGGCAGGAGATCCAGCGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAACAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGCGTACGCGAACGAACCAGGCGTAGATATCACCAAACGCCGCTTGGCCTGCTTCCAGACCGACAAATCCAGGc                                                                                                                                                                           >  1:39172/1‑251 (MQ=255)                                                                                      gagcgccgaccGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCCAGCTGTTCCAGCGGCCAGCTGAGGACGCGACCGCACCAGGCGTAGATATCACCAAACGCCGATTGGACAGCTTCCAGACCGATACATCCAGGCACCCCCCTGCCATCAAc                                                                                                                                                          >  2:271667/10‑251 (MQ=255)                                                                                         cgacgaccGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTg                                                                                                                                                       <  1:318852/245‑1 (MQ=255)                                                                                            cgaccGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGCTTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCCGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGAcc                                                                                                                                                    >  2:131143/4‑251 (MQ=255)                                                                                             gaccGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCg                                                                                                                                                   <  2:117777/249‑1 (MQ=255)                                                                                                    aaaCCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATAc                                                                                                                                            <  2:265826/251‑1 (MQ=255)                                                                                                                        gCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCa                                                                                                                        <  1:295499/251‑1 (MQ=255)                                                                                                                                   agagACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCACGCTCGCCAACGCTCTGttt                                                                                                             >  1:254249/1‑251 (MQ=255)                                                                                                                                                                cAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGaa                                                                                 <  2:146259/250‑1 (MQ=255)                                                                                                                                                                 aGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGCTTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGacgt                                                                               >  2:243390/1‑248 (MQ=255)                                                                                                                                                                 aGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGt                                                                               >  1:52357/1‑251 (MQ=255)                                                                                                                                                                          aGCAGTTGTTTCTGGCTGGCGTTGATTTGCGCTTCCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAAc                                                                      <  1:8260/251‑1 (MQ=255)                                                                                                                                                                              gttgttTCTGGCTGGCGTTGATTTGCGCTTTCAGTTCCGGCTGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAACTACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACtttt                                                                  >  2:305819/1‑251 (MQ=255)                                                                                                                                                                                       ggctggcGGTGATTTGCGCTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGc                                                          <  1:235316/250‑1 (MQ=255)                                                                                                                                                                                                               gTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACt                                 >  2:78032/1‑251 (MQ=255)                                                                                                                                                                                                                         cTGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCatat                       >  1:26001/1‑251 (MQ=255)                                                                                                                                                                                                                          tGGGCGGCAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACTGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATg                      >  2:222163/1‑251 (MQ=255)                                                                                                                                                                                                                               ggcAAGCTGTTCCAGCGGCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGt                 <  2:83145/251‑1 (MQ=255)                                                                                                                                                                                                                                              gggCCAGCTGAGTACGCGACCGAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGGCGCCgg  <  2:52357/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   AATCAAACCGCCGAACAGCAGCGGAGCGTCGGTAGCGAGGTTAAGATCGGTAATCACCCCTTTCAGGCGTTGGTTAGCGTTCGGTGTGCGGCGGCCGTTAAACCAGTCGAGCACCACCGGCAGGTGATCCAGAGACGGATTTTTGGCCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCAACTGCGCCCATATGGCAGTCAAACGCGCCGCCGG  >  NC_000913/68756‑69243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑