Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	66,823	G→T	intergenic (‑273/+12)	araD ← / ← araA	L‑ribulose‑5‑phosphate 4‑epimerase AraD/L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	66,823	0	G	T	100.0%	87.1 / NA	34	intergenic (‑273/+12)	araD/araA	L‑ribulose‑5‑phosphate 4‑epimerase AraD/L‑arabinose isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (16/18);  total (16/18)

GGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCG‑CAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATGCGGCTACTTAGCGACGAAACCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGTGTGTCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATT  >  NC_000913/66587‑67062                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                ggCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCG‑GAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACtt                                                                                                                                                                                                                                    >  2:241561/1‑250 (MQ=255)  gCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCG‑GAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAg                                                                                                                                                                                                                                  <  1:248199/251‑1 (MQ=255)                             gATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCG‑GAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGt                                                                                                                                                                                                         <  1:99404/249‑1 (MQ=18)                              aTAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCG‑GAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGGTAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTcc                                                                                                                                                                                                      >  1:240240/1‑251 (MQ=18)                                aaGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCG‑GAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCcag                                                                                                                                                                                                    <  1:138992/251‑1 (MQ=18)                                      cgcCAGCGTCGAATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCG‑GAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATGCACTTCGTTCcagcgcagc                                                                                                                                                                                              <  2:181840/251‑1 (MQ=18)                                                    cAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCG‑GAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGGCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGCAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGc                                                                                                                                                                                >  2:245600/1‑251 (MQ=18)                                                           tGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCG‑GAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAACCGCTGGccgg                                                                                                                                                                         >  2:138804/1‑248 (MQ=18)                                                           tGAAGGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCG‑GAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCat                                                                                                                                                                          <  2:170008/250‑2 (MQ=18)                                                                gCCGGATGCGCTTTGCTTATCCTGCCTACAAAATCG‑GAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTATCGACGAAATCCGTAATACACGTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAgtcgagt                                                                                                                                                                    <  2:51700/251‑7 (MQ=18)                                                                                 tATCCGGCATACAAAATCGGCA‑CGCGTAGGCCTGCTAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGAGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTCCTTAGCGACGAAATCCGTACTACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGCTAACAATCAcc                                                                                                                                                    >  2:116385/1‑250 (MQ=18)                                                                                  aTCCGGCCTACAAAATCG‑GAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGt                                                                                                                                                  <  1:241561/251‑1 (MQ=21)                                                                                      ggCCTACAAAATCG‑GAGCGCGTAGGCCTTATAAGACGCGTCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTGGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTTATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCTACTACTTAGCGACGAAATCCGTAATACACTTCGGTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGTGGGTATCGTTATCAATCACCTTGAtt                                                                                                                                              <  2:282992/251‑1 (MQ=18)                                                                                             caaagtcggactgcgTAGGCCTGATAAGACGTGCCTGTGTACCATCAGGCTGTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACGCTTCGTTCCAGCGCATCGTGTCTTTAAACGCTGGCATGCGGGTATCGTTATCAATCACCGTGATTTCAATg                                                                                                                                        <  2:190500/239‑1 (MQ=21)                                                                                                      aacgcgTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCAtc                                                                                                                               <  2:176297/249‑1 (MQ=25)                                                                                                           gTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGc                                                                                                                          >  1:281273/1‑251 (MQ=25)                                                                                                                   gATAAGACGCGCCTGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCGACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTAGCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGaactg                                                                                                                    <  2:210989/249‑4 (MQ=25)                                                                                                                   gATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGaactg                                                                                                                    <  2:281273/249‑4 (MQ=25)                                                                                                                        gACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCata                                                                                                             <  1:143835/251‑1 (MQ=25)                                                                                                                            cgcCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGGTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCgg                                                                                                         >  1:104158/1‑250 (MQ=25)                                                                                                                               cAGCTTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCCACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTACATCTCGGCGAACTGGCGCATATCGTTGa                                                                                                      <  2:85971/251‑1 (MQ=25)                                                                                                                                               gTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGATCGTTATCAATCACCGGGATTTTAATGTCGTGCATCCCGGCGAACCGGCGCCTATCGTTTAGGTTTAGCGCATGGcc                                                                                      >  2:175021/1‑250 (MQ=25)                                                                                                                                                      gCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACGGGCGCATATCGTTGAGGGTCAGCGCATGGCTGAAGAc                                                                                <  2:180317/250‑1 (MQ=37)                                                                                                                                                       ccGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGATCGGTATCAATCACCGGGGTTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGCTGAGGTTCAGCGCCTGGCCGAAGCCgg                                                                              >  2:284497/1‑251 (MQ=25)                                                                                                                                                             gcgcTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGGTCAGCGCATGGCTGAAGACGGTATggg                                                                        >  1:200112/1‑250 (MQ=38)                                                                                                                                                             gcgcTTTGCTTAACCGGCCTACACAATAGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAAAGCTCGCAGGCGGGTATCGTTATCCATCACCGTGATTTCAATGTCGGGCATCTCGGCGAAACTGCGCATATCGTTGAGGTTCAGCGCACGGCTGAAGACGGTCTGGt                                                                        >  2:39374/1‑251 (MQ=255)                                                                                                                                                              cgcTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCATCGCGTCGTTAAACGCTGGCAGGCGGGTATCGTTATCAATTACCGTGATTGCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTg                                                                       <  1:299076/251‑1 (MQ=37)                                                                                                                                                                              ccTACAAAATCTCATCGTGGAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCATGCGGGTATCGTTATCAATCGCTGTGATTTCAAGGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTGCAGCGCATGGCTGAATACGGTATGGGGCGGGCAACCTGCGAgg                                                       <  2:203387/251‑1 (MQ=255)                                                                                                                                                                               cTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTTTCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGGTGAGGTTCAGCGCATGGCTGAAGACGGTATGGGGCGCGCCACCAGCggggg                                                      >  2:229452/1‑247 (MQ=255)                                                                                                                                                                                 aCAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGGGCGCGCCACCAGCGAGGATc                                                    >  1:95748/1‑251 (MQ=255)                                                                                                                                                                                     aaTCGCAGCGTGTAGGCCTGATAAGACGCGTCCGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTTTCGTTATAAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAAATGGCGCATATCGTTGAGGGTCAGCGCATGGCTGAAGAAGGGATGGTGCGCGCCACCAGAGAGGATACACg                                                >  2:54290/1‑251 (MQ=255)                                                                                                                                                                                                        gATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCa                              <  2:2163/250‑1 (MQ=255)                                                                                                                                                                                                                       cGTCGAATCAGGCGTTACACACCTGATGCGACTACCTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGGACTGGCGCATATCGTTGAGGTTCAGCGCACGGCTGAAGACGGTATGGTGCGCGCCCCCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCttt              >  1:272495/1‑251 (MQ=255)                                                                                                                                                                                                                                   cGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCCAGGATCCCCGCTTCGGAAGCAGTTGGCAGATCCGGGTGCGCTTTCCACAGCGCAtt  >  2:219762/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                GGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCG‑CAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATGCGGCTACTTAGCGACGAAACCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGTGTGTCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATT  >  NC_000913/66587‑67062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑