Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,561,368	C→T	P147S (CCG→TCG)	kptA →	RNA 2'‑phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,561,368	0	C	T	100.0%	95.6 / NA	28	P147S (CCG→TCG)	kptA	RNA 2'‑phosphotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/15);  total (13/15)

CAGCGATAAAAAGCGTTTTAGTTATTCCAGTGATGGTAGGTGCATCCGCGCGGTTCAGGGGCATTCGACTTCGCAGGTCGCTATTTCCTTTGCCGAAAAAAC‑ACCGCCACAGTTTCTCTACCACGGTACGGCAAGCCGTTTCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATG  >  NC_000913/4561121‑4561600                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                         cgctcttcatatttgTTTTATTAATTCCGGTGATGTTTGTTGCACCCGCGCGGTGAGGGGGCATTCGACTTCGCAGGTCGTTATTTCCTTGGCCGAAAAAAC‑ACCGCCACAGTTTCTCTACCACGGTACGGCAAGCCGTTTCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCgg                                                                                                                                                                                                                                       <  2:226057/237‑1 (MQ=255)               gTTTTAGTTATTCCAGTGATGGTAGGTGCATCCGCGCGGTTCAGGGGCATTCGACTTCGCAGGTCGCTATTTCCTTTGCCGAAAAAAC‑ACCGCCACAGTTTCTCTACCACGGTACGGCAAGCCGTTTCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCgg                                                                                                                                                                                                                                       >  1:226057/1‑237 (MQ=255)               gTTTTAGTTATTCCAGTGATGGTAGGTGCATCCGCGCGGTTCAGGGGCATTCGACTTCGCAGGTCGCTATTTCCTTTGCCGAAAAAAC‑ACCGCCACAGTTTCTCTACAACGGTACGGCAAGCCGTTTCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCgg                                                                                                                                                                                                                                       >  1:226045/1‑237 (MQ=255)                   tAGTTTTTCCATTGATGGTAGTTGCATCCGCGCGTTTCAGGGGCATTAGACTTCGCAGGTCGCTATTTCTTTTCCATAAAAAAC‑ACCGCCACAGTTTCTCTACCACGGTACGTCAATCCGTTTCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACTGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTGAACCGTCaaa                                                                                                                                                                                                                      <  1:46746/250‑1 (MQ=255)                                 atggtactcgCATCCGCGCGCTTCAGCCGCAACCGCCTTCGCAGGTCCCTATTTCCTTCGCCGACAAAAC‑ACCGCCACAGTTTCTCTACCACGGTACGGCAAGCCGTTTCCTTGACGAGATAAAAAACCAGGGGCTGATTCCAGGTGAGCGCCACTACCAACCTCTCTCTGCTGCTGAAGCAACGGCACGCAAAGTGGGCGCCACACACGAATCGTCGGTTATTTTAACCGTCAAACCAAAGGAAATGGcc                                                                                                                                                                                                       >  2:36898/10‑250 (MQ=255)                                                           ggacATTCGTCTCCGCAGGTCGCTTTTCCCGTTCCCGAAAAACC‑ACCGCCACAGTTTCTCTACCACGGTACGGCAAGCCGTTTCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGc                                                                                                                                                                             <  2:321407/248‑1 (MQ=255)                                                                ttcacctccgaaGGTCGCTATTTCCTTGCCCAAAAAAAC‑CCCGCCACAGTTTCTCTACCACGGTACGGCAAGCCGTTTCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGaa                                                                                                                                                                         <  1:166268/239‑1 (MQ=255)                                                                                    tttCCTT‑GCCAAAAAAACCACCGCCACAGTTTCTCTACCACGGTACGGCAAGCCGTTTCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATc                                                                                                                                                     <  2:301940/250‑1 (MQ=255)                                                                                                                aGTTTCTCTACCACGGTACGGCAAGCCGTTTCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTgg                                                                                                                         >  1:58947/1‑251 (MQ=255)                                                                                                                       ctACCACGGTACGGCAAGCCGTTTCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAgg                                                                                                                    <  2:139546/249‑1 (MQ=255)                                                                                                                                          cGTTTCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGCCATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGa                                                                                               <  1:54562/251‑1 (MQ=255)                                                                                                                                                  tgccgctctcccaaaaCAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCACCGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGCCCACTTGt                                                                                       >  1:290378/13‑251 (MQ=255)                                                                                                                                                    aCGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGGTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAg                                                                                     <  1:188364/251‑1 (MQ=255)                                                                                                                                                    aCGAGATAAAAAAAAAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCGGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGCAAGGGAAACTTCCCTCATGAAGTGACCACTTGt                                                                                       >  1:166625/1‑249 (MQ=255)                                                                                                                                                                                    gAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCGTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCgca                                                      <  2:119436/250‑1 (MQ=255)                                                                                                                                                                                     aGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCgcagc                                                    <  1:155740/251‑1 (MQ=255)                                                                                                                                                                                          cACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATaa                                               <  1:231865/251‑1 (MQ=255)                                                                                                                                                                                              aCGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAaac                                             <  1:314313/249‑1 (MQ=255)                                                                                                                                                                                                        ctctGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAAcgccgcc                                    <  1:316506/248‑1 (MQ=255)                                                                                                                                                                                                            gctgATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCcgccgcgcc                               <  2:114830/249‑1 (MQ=255)                                                                                                                                                                                                                atgaAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGACAATTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGACGCag                           >  2:23349/1‑249 (MQ=255)                                                                                                                                                                                                                     gCAACGGCACGCAAAGTCGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGAGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTgg                    >  1:256471/1‑251 (MQ=255)                                                                                                                                                                                                                       aaCGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGgcg                  >  2:69127/1‑251 (MQ=255)                                                                                                                                                                                                                            cACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACAGTCAAAGCACCGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGc             >  1:29196/1‑251 (MQ=255)                                                                                                                                                                                                                              cGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAACACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCa            >  1:122998/1‑250 (MQ=255)                                                                                                                                                                                                                               gCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCa            >  2:259122/1‑249 (MQ=255)                                                                                                                                                                                                                                   aGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGcc      <  1:87700/251‑1 (MQ=255)                                                                                                                                                                                                                                       ggAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATg  >  1:264143/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                         CAGCGATAAAAAGCGTTTTAGTTATTCCAGTGATGGTAGGTGCATCCGCGCGGTTCAGGGGCATTCGACTTCGCAGGTCGCTATTTCCTTTGCCGAAAAAAC‑ACCGCCACAGTTTCTCTACCACGGTACGGCAAGCCGTTTCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATG  >  NC_000913/4561121‑4561600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑