Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,824,426 G→C R676P (CGT→CCT)  spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,824,4260GC95.5% 63.8 / ‑2.8 22R676P (CGT→CCT) spoTbifunctional (p)ppGpp synthase/hydrolase SpoT
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (15/6);  total (16/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

GTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGAAAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTC  >  NC_000913/3824207‑3824667
                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                 
gTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGAAAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCCTGACCGTGTGCATCTGGCGAATATCATgcgc                                                                                                                                                                                                                    <  1:236392/251‑1 (MQ=255)
   gCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGAAAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCCTGACCGTGTGCATCTGGCGAATATCATGCGCaa                                                                                                                                                                                                                  >  1:51908/1‑250 (MQ=255)
          gAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGAAAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTGGCGAATATCATGCGCAAAATCcgcg                                                                                                                                                                                                          >  1:10296/1‑251 (MQ=255)
                 ccAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGAAAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCCTGACCGTGTGCATCTGGCGAATATCATGCGCAAAATCCGCGTGATGcc                                                                                                                                                                                                   >  2:316734/1‑251 (MQ=255)
                          tttATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGAAAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCCTGACCGTGTGCATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGAt                                                                                                                                                                                          >  1:28878/1‑251 (MQ=255)
                                       aaTGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGAAAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCCTGACCGTGTGCATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACccg                                                                                                                                                                              >  1:101903/1‑250 (MQ=255)
                                        aTGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGAAAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCCTGACCGTGTGCATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACccga                                                                                                                                                                             >  2:303294/1‑250 (MQ=255)
                                           ggATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGAAAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCCTGACCGTGTGCATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACccgaaacc                                                                                                                                                                         <  1:171857/251‑1 (MQ=255)
                                                       ggCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGAAAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCCTGACCGTGTGCATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGtt                                                                                                                                                             <  1:221072/251‑1 (MQ=255)
                                                                                          tGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGGTTGAATACGGAAGAGAAAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCCTGACCGTGTGCATCTGGCGAATATCCTGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAAAACGTTATGCACGCATCTGCGa                                                                                                                           >  2:271736/1‑250 (MQ=255)
                                                                                                                   aaaCCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGAAAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCCTGACCGTGTGCATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATc                                                                                                 >  1:168445/1‑251 (MQ=255)
                                                                                                                     aCCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGAAAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCCTGACCGTGTGCATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCATGCGGCAGCCTGATCTg                                                                                               >  2:115394/1‑251 (MQ=255)
                                                                                                                                     tAACACCACTACTTCGAATATTCAAAGTTTGAATTCGGAAGCGAAATATTGTCGCGTCTACAGCGCCTTTTTTCGTCTGACCGCTCCTGACCCTGTGCATCTGGCGAATAGCATGCGCCAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTAGGAACCCATCTCGTTATGCACGCATCAGCGAAATGCTCACCAGGCTGCAGCCTGATCTGACGGTCTGCATGGAg                                                                                <  2:317812/250‑1 (MQ=255)
                                                                                                                                         cccacGACTGCGAATATGCAAAGTTTGAATACGGAAGAGAAAGATGGTCGCGTCTACAGCGCATATATTCGTCTGACCGCTCCTTGCAGTGTGCATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAACGGCACCCGAAACCGAAATTAATGTTTTATGAGCCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCACGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGt                                                                           <  2:282017/250‑1 (MQ=255)
                                                                                                                                                     aaTATTCAAAGTTTGAATACGGAAGAGAAAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCCTGACCGTGTGCATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTc                                                                >  1:228795/1‑250 (MQ=255)
                                                                                                                                                           cAAAGTTTGAATACGGAAGAGAAAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCCTGACCGTGTGCATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAAct                                                          <  2:100942/250‑2 (MQ=255)
                                                                                                                                                                       aCGGAAGAGAAAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCCTGACCGTGTGCATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGattat                                             >  1:45529/1‑251 (MQ=255)
                                                                                                                                                                            agagAAAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCCTGACCGTGTGCATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGt                                         >  1:29407/1‑250 (MQ=255)
                                                                                                                                                                               gaAAGATGGTCGCGTCTCCAGCGCCTTTATTCGTCTGACCGCTCCTGACCGTGTGCATCTGGCGAATATCATGCGCCAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCg                                     >  2:154008/1‑251 (MQ=255)
                                                                                                                                                                                                  aGCGCCTTTATTCGTCTGACCGCTCCTGACCGTGTGCATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAc                   >  2:120982/1‑250 (MQ=255)
                                                                                                                                                                                                    cgcCTTTATTCGTCTGACCGCTCCTGACCGTGTGCCTCTGGCGAATATCATGAGCAAAATCCACGTCCTGCCCGACGTGATTAAAGTAACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGCTCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATg                >  2:34152/1‑251 (MQ=255)
                                                                                                                                                                                                                  tGACCGCTCCTGACCGTGTGCATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAAAACGTTATGCACGCATTTGCGAAATGCTCGCCAGGCGGCAGCCTGATATGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTc  >  2:214927/1‑251 (MQ=255)
                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                 
GTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACGGAAGAGAAAGATGGTCGCGTCTACAGCGCCTTTATTCGTCTGACCGCTCGTGACCGTGTGCATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTC  >  NC_000913/3824207‑3824667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: