Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,682,515	C→T	M311I (ATG→ATA)	dctA ←	C4 dicarboxylate/orotate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,682,515	0	C	T	100.0%	90.4 / NA	27	M311I (ATG→ATA)	dctA	C4 dicarboxylate/orotate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/9);  total (18/9)

CGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACAC‑CGCCGCCATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACCACAAACAGG  >  NC_000913/3682274‑3682755                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 cGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACAC‑CGCCGCTATTGTCAgg                                                                                                                                                                                                                                         >  1:286594/1‑251 (MQ=255)       cGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACAC‑CGCCGCTATTGTCAGGTATATc                                                                                                                                                                                                                                   >  1:87476/1‑251 (MQ=255)        gACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACAC‑CGCCGCTATTGTCAGGTATATCg                                                                                                                                                                                                                                  >  1:11374/1‑251 (MQ=255)                     aGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACAC‑CGCCGCTATTGTCAGGTATATCGATGTGCCATCa                                                                                                                                                                                                                       >  1:59955/1‑249 (MQ=255)                                    gACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACAC‑CGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGcc                                                                                                                                                                                                      >  1:165036/1‑251 (MQ=255)                                                  gATACCGAGGATCAGCGCCAGACCCGCTACCGGCAACTGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACAC‑CGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGAcc                                                                                                                                                                                         >  2:14265/1‑250 (MQ=255)                                                  gATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACAC‑CGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGAcc                                                                                                                                                                                         >  2:78485/1‑250 (MQ=255)                                                                     aGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACAC‑CGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACg                                                                                                                                                                     >  2:141903/1‑251 (MQ=255)                                                                                       ggcccccccGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTGAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACAC‑CGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCATCCGAGTTTCTCCAt                                                                                                                                                   <  1:244304/244‑1 (MQ=255)                                                                                          cccACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACAC‑CGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCtt                                                                                                                                                >  2:240076/1‑251 (MQ=255)                                                                                                                                 cTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACAC‑CGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGt                                                                                                         >  1:43424/1‑251 (MQ=255)                                                                                                                                     ccGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACAC‑CGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTcccc                                                                                                     >  1:6258/1‑251 (MQ=255)                                                                                                                                          cccccgcccgccCCTTTAAAAGAAACCACCAACAGGATAAACGGCGGGATTTGGTGGCCGATACCCTTCTGCTTGTTAGTGGCCTGGGCGATAAACAC‑CGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTAc                                                                                                <  2:186916/244‑1 (MQ=255)                                                                                                                                              cTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACAC‑CGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATc                                                                                            >  1:127977/1‑251 (MQ=255)                                                                                                                                                          aagaagaAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCGGGGCGAAAAACAC‑CGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCa                                                                                <  1:240076/250‑1 (MQ=255)                                                                                                                                                               gaaAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACAC‑CGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGCAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACggct                                                                           >  2:248138/1‑249 (MQ=255)                                                                                                                                                                     agcaACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACAC‑CGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCg                                                                     >  1:58832/1‑251 (MQ=255)                                                                                                                                                                            cGATGAACGGCGTGGTTTGGATGACGATATCCATCTGACGTTTAGTGGCCGGGGCGA‑AAACACACGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTGCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAAGCAGCAGTTCTTCACGGATGTAGCGGATAAAt                                                              <  2:44112/251‑1 (MQ=255)                                                                                                                                                                                tAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACAC‑CGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTg                                                           >  1:11202/1‑250 (MQ=255)                                                                                                                                                                                aaaCAGCGTGATTTGGTGGACAATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACAC‑CGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGa                                                          <  1:67432/250‑1 (MQ=255)                                                                                                                                                                                        tGATTTGGTGGACGATACCCATCTGACTGTTAGTGCCCTGGGCGATAAACAC‑CGCCGCTATTGTCAGGTATATCGCTGTGCCATCAAGGTTAACCGAGTAGACTGTCGGGATGACCAGCCCCACCACCGATTTACGACAGCCGCGTTTCTCCATCTTGTCGAGCATACGCGGCCGCGCCGACTCGGAAGATGAAGCCCCCAGTACAATCAGCAGTTCTTCCCGGATGTAGCGGATAACTTTGAAGCTACtt                                                  >  2:112277/1‑250 (MQ=255)                                                                                                                                                                                                 ggACGATATCCATCTGACTGTTAGTGGCCTGGGCGAAAAACAC‑CGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTc                                         <  1:138144/251‑1 (MQ=255)                                                                                                                                                                                                        acccATCGGACTGTTGGTGGCGTGGGCGATAAACAC‑CGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGAAACTGAAACCAGTCGCTTTAg                                  <  2:286594/249‑1 (MQ=255)                                                                                                                                                                                                                                   gATAAACAC‑CGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACcaca        >  2:112834/1‑250 (MQ=255)                                                                                                                                                                                                                                        ccac‑CGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACCACAAACAgg  <  2:127977/250‑1 (MQ=255)                                                                                                                                                                                                                                        acac‑CGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACCACAAACAgg  <  1:239923/251‑1 (MQ=255)                                                                                                                                                                                                                                         cac‑CGCCGCTCTTGTCAGGTATATCGATGTGCCATCCAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACCGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCACAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCACAACCACAAACAgg  >  2:149549/1‑250 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 CGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACAC‑CGCCGCCATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACCACAAACAGG  >  NC_000913/3682274‑3682755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑