Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,629,921	C→T	E228K (GAG→AAG)	yhiI ←	putative membrane fusion protein YhiI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,629,921	0	C	T	100.0%	210.4 / NA	59	E228K (GAG→AAG)	yhiI	putative membrane fusion protein YhiI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/27);  total (32/27)

TTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTCGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGC  >  NC_000913/3629685‑3630164                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    gttCAGCCGTTCATCGTTGGTTTCGGCGGTTTTTGGCTGGAACTGGGCGACTCTGGCGACAAAACTGATGGTTGCAGAAATCCGCAGATCTGGCGCGGCATCGAGGATGAGCCGGGCTTCCCCGCCAATTTCCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACt                                                                                                                                                                                                                                       <  2:177044/250‑1 (MQ=255)   tCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGc                                                                                                                                                                                                                                     >  1:129994/1‑251 (MQ=255)    cAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGc                                                                                                                                                                                                                                     >  1:8412/1‑250 (MQ=255)       ccGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCAcgc                                                                                                                                                                                                                                 >  1:9783/1‑251 (MQ=255)       ccGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCAcgc                                                                                                                                                                                                                                 >  1:111981/1‑251 (MQ=255)         gTTCAGCGCTGGTTTCGAAGGGTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTGAGCGAGCCCGCCGGTTCGGTTGGCAGGAAGAAAGGCATATAGACGTCGCTTAGATCGACCATATTCAGCACCAGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGTCAACCCGATACTGCAcgcgg                                                                                                                                                                                                                               >  2:58949/1‑250 (MQ=255)           tCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGcgtcc                                                                                                                                                                                                                             >  1:72569/1‑251 (MQ=255)            cATCGCTGGTTTGGACGGTTTTTGGCGTGAACTGGGCGACACGGGCGACAAAACGGATGGTTGCAGGAATACGCAGATCGGGCGCGGCATCGGGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGcgtcc                                                                                                                                                                                                                             <  2:289437/250‑1 (MQ=255)                   ggTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCAcgcg                                                                                                                                                                                                                     >  1:278967/1‑251 (MQ=255)                   ggTTTCGACGGTTTTGGGCGTGAACTGGGCGACCCTGGCAACAAACCTGTGGGTTGCAGGAAGACGAAGATCTGCCGCGCCATCGAGGATCAGCCGGGCTTCCCCGCCCAGTTCCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCAcgcg                                                                                                                                                                                                                     <  2:200527/251‑1 (MQ=255)                        cGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGAACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCt                                                                                                                                                                                                                >  1:225181/1‑251 (MQ=255)                                   ggCGTGAACTGGGCGACACTGGCGACAAACCTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCATCACCGCCCAGTTTCAGAGTGCCCGCCAGTTCGGTTGGCAGGCAGAAAGACATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCAATTCGCCTGGCTTGGCAACACGATACTGCAAGCGTCCGTCACGCGGGGCTTTCAGTTCGCt                                                                                                                                                                                                     >  1:202637/1‑251 (MQ=255)                                    gCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTg                                                                                                                                                                                                    >  1:239037/1‑251 (MQ=255)                                              ggCGACACTGGCGAAAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGatat                                                                                                                                                                                          >  2:41269/1‑251 (MQ=255)                                                  acacTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCAAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGc                                                                                                                                                                                      >  1:297393/1‑251 (MQ=255)                                                             aaaaCTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCAGCGAGGATCAGCCGGGCTTCCCCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGcc                                                                                                                                                                           <  1:12887/251‑1 (MQ=255)                                                              aaaCTGATGGTTGCGGGAATACGCAGATCGGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCg                                                                                                                                                                          <  1:18630/251‑1 (MQ=255)                                                                   gATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGCGGATCAGCCGGGCTTCACCGCACAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATCGACGTCGCTGAGATCGACCATATTCACCACACGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGAGGCAATGCGCCGTTc                                                                                                                                                                       >  1:48944/1‑249 (MQ=255)                                                                   gATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTc                                                                                                                                                                       <  1:159047/249‑1 (MQ=255)                                                                                 tACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCt                                                                                                                                                       >  1:24219/1‑251 (MQ=255)                                                                                         tCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCCTCGAcgcg                                                                                                                                               >  2:291449/1‑251 (MQ=255)                                                                                           tGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCa                                                                                                                                                                                                                                    >  2:94905/1‑164 (MQ=255)                                                                                           tGGCGCGGCAGCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCa                                                                                                                                                                                                                                    <  1:94905/164‑1 (MQ=255)                                                                                            ggCGCGCCTTCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCggg                                                                                                                                             <  1:112835/250‑1 (MQ=255)                                                                                                     gcGAGGATCAGCCGGGCTTCCCCGCCCATTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTg                                                                                                                                   <  1:276911/250‑1 (MQ=255)                                                                                                     gcGAGGATCACCCGGGCTTCCCCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTg                                                                                                                                   <  2:145760/250‑1 (MQ=255)                                                                                                       gAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTTGGCCGCCTCGCCGCggggtgtcgcctga                                                                                                                                  >  2:232649/1‑239 (MQ=255)                                                                                                        aGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAAt                                                                                                                                <  1:158206/251‑1 (MQ=255)                                                                                                         ggATCAGCCGGGCTTCCCCGCCCAGTTTCAGCTTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATg                                                                                                                               <  1:39716/251‑1 (MQ=255)                                                                                                          gATCAGCCGGGCTTCCCCCCCAAGTTTCAGCTTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCCCCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCTGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGa                                                                                                                              <  2:107454/251‑1 (MQ=255)                                                                                                                  gggCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTTCGCCTGAATGATATTGgtg                                                                                                                      >  1:46144/1‑251 (MQ=255)                                                                                                                            gCCCATTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCtt                                                                                                            <  2:11825/251‑1 (MQ=255)                                                                                                                             cccAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAACACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCCTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTc                                                                                                           >  2:157114/1‑251 (MQ=255)                                                                                                                                acttccaccGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGGTCAGTGTCTTGGGCCGCTTCGACGCGGTTTTGCGCCTGAATGATATTGCTTCGTGCCGCTTCtat                                                                                                        <  1:54338/243‑1 (MQ=255)                                                                                                                                   tgcggcggtcccGCCGGTTCGGGTGGCAGGAAGAAAGTCAAATAGCGGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAg                                                                                                      <  2:309454/241‑1 (MQ=255)                                                                                                                                         gTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGGGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCttt                                                                                               >  1:179765/1‑251 (MQ=255)                                                                                                                                           gCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCCTCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGATTCGACGCGGGTTTTCGCCTGCATGATATTGGTGCGTGCCGCGTCTATAGCCGCttt                                                                                               >  2:252195/1‑249 (MQ=255)                                                                                                                                                   gTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCTACGAGGGGTTTCGCCTGAATGATATTGGTGCGTGGCGCTTCTCTTGCCGCTTTAGAAGACGAt                                                                                     >  1:28111/1‑251 (MQ=255)                                                                                                                                                     tCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCCCCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATa                                                                                    <  1:232148/250‑1 (MQ=255)                                                                                                                                                      cGGTTGGCAGGAAGAAATTCAAATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGGCGCGGGTTTGCGCCTGAAGGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATAc                                                                                   <  1:150892/250‑1 (MQ=255)                                                                                                                                                        gTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTg                                                                                >  1:1872/1‑251 (MQ=255)                                                                                                                                                                gaagaaAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGGGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTTAGCTTTCg                                                                        >  2:95948/1‑251 (MQ=255)                                                                                                                                                                    aaaGTCATATAGACGTCGCTGAGATCGCCCATATTCAGCCCCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGa                                                                    <  2:72569/251‑1 (MQ=255)                                                                                                                                                                                      cTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGGGTGCCGCTTCTATAGCCGCTTTAGAAGCCGGTACCTGAGCTTTTGCCGATTCCaacccaccccgggc                                                  >  2:135726/1‑238 (MQ=255)                                                                                                                                                                                              gACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCGTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGAGACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAg                                          <  1:157744/251‑1 (MQ=255)                                                                                                                                                                                               cccATATCCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCACCCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTTATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAgc                                         <  2:81219/250‑1 (MQ=255)                                                                                                                                                                                               aCCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGCTACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCCTCGACGCGGGGTTGCGCCTGAATGATATTGGTGCGTTCCGCTTCTTTAGCCGCTTTAGAAACCGATACCTGAGCCTTCCCCGATTCCAGCGCAGTTCGGGCGCtctccgc                                         >  2:112007/1‑248 (MQ=255)                                                                                                                                                                                                  atatTCAGCCCCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAgcggc                                      <  1:44737/251‑1 (MQ=255)                                                                                                                                                                                                    atTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTGCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAgcggcg                                     <  1:192620/250‑1 (MQ=255)                                                                                                                                                                                                          cACCCGACCGCCGGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGAt                              <  2:129994/251‑1 (MQ=255)                                                                                                                                                                                                             ccGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCCTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTTTTGCCGCTTTAGAAGCCGAAACCTGAGCTTTCGCCGATTCCAGCGCAGCCCCGGCGCTCTCAGCGGCGgcgccgtcgg                           >  2:103820/1‑245 (MQ=255)                                                                                                                                                                                                                aCCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGGTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCCATACCTGAGCTTTCGCCGCTTCCAGCGCAGCTCGGGCGCTCTCCGCGGCGGCGCGATCGTCa                         >  2:91865/1‑250 (MQ=255)                                                                                                                                                                                                                   gCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCCTCTATAGCCGCTTTAGAAGCCGATACCTGTGCTTTCCCCGATTCCAGCGCACCTCCGGCGCTCTCACCGGCGGCGCGATCgccaacc                      >  1:10053/1‑244 (MQ=255)                                                                                                                                                                                                                             aGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGAAACCTGAGCTTTCGCCGATTCCAGCGCAGCCCGGGCCCTCTCAGCGGCGGCGCGATCGTCATCCAGCGGTTgcgc           >  2:3320/1‑251 (MQ=255)                                                                                                                                                                                                                                   tCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGaaa      <  1:41269/250‑1 (MQ=255)                                                                                                                                                                                                                                    cGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCTGATACCTGAGCTTTCGCCGCTTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCCTCCAGCTGTTGCCCAGaaaa     >  2:262375/1‑249 (MQ=255)                                                                                                                                                                                                                                    cGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCCCCGGCGCTCTCAGCGGCGGCGCGATCGTCATCCACCTGGTGCGCAGAAAt     >  2:87715/1‑250 (MQ=255)                                                                                                                                                                                                                                      ccTGGCTTGGCAACCCGATACTGCACGCGTCCGTCTCGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGGCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCACAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGc  <  1:194314/251‑1 (MQ=255)                                                                                                                                                                                                                                      ccTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTTTCATCGATATCTGCGGCAATGCGCCGTTCAGTGTTTTGTGCCGCTGCGACGCGGGTTTGCGCCGGAATGATATTGGTGCTTGCCGCTGCTATAGCCGTTTTAGAAGCCGATGCCTGAGGTTTCGCCGATTCCAGCTCAGCTCGGGCGCTCGCATCGGCGTCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGc  <  1:194326/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    TTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTCGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGC  >  NC_000913/3629685‑3630164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑