Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,788	A→T	intergenic (‑8/+67)	polB ← / ← araD	DNA polymerase II/L‑ribulose‑5‑phosphate 4‑epimerase AraD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,788	0	A	T	100.0%	79.7 / NA	24	intergenic (‑8/+67)	polB/araD	DNA polymerase II/L‑ribulose‑5‑phosphate 4‑epimerase AraD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (15/9);  total (15/9)

ATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAAC‑GGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATG  >  NC_000913/65544‑66027                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                aTACACCGGCTTGCGCTGAAACCCCTTTAACGCCAGCTGTTTCAGGCGAACTCCTTGTTCCCCCTGCAAAATATGCTGCGCGCGGGTCCCCTGAGCGGCGGGAATAACCGCCACGGTCGCTTCCGGTGCAAGCGTACCCTGCAAC‑GCCCCTTTGTCCTTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCTGGTTGTCCCGCCAGTGTCTTTTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATc                                                                                                                                                                                                                                           <  1:178533/251‑1 (MQ=255)  tACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAAC‑GGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCCTGCGGGGGGTCCCGCCCGGGTCGGGGTAAAATAAAAACCGCCCGCGCCCCCCTGTAATTCCATc                                                                                                                                                                                                                                           >  1:87533/1‑250 (MQ=255)       ggggtGGCGGTGAAAATCCTTTAACGCCAGCGTTGTCAGGCGAACGCATTGTTCACCCTGCAAAAGATTCGGAGCGGGGGGAACCTGATGGGGGGAAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAAC‑GGCCCGTTGTCCGTCCCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGGCGGGTTAAGATAAAACCTGCCTGCGCCTCGCTGTAATTCCATCaaaaaa                                                                                                                                                                                                                                     <  2:101126/247‑1 (MQ=255)                                   aGCGGTGTCAGGCGAGCGCCCTGTTCCCCCTACAAAATATGCTGGGCGCGGGGACCCTGATGGGCGGTAATAAACGCCACGGACTCTGGCGGTGCAAGCGTAACCTGCACC‑GGCCCGTTGTCCGTCGCCACCCAGAAGGAGACTTCGGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGtt                                                                                                                                                                                                         <  2:118799/251‑1 (MQ=255)                                                  acgCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAAC‑GGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGtt                                                                                                                                                                                          <  1:128281/249‑1 (MQ=255)                                                                           gCTGAGCGCGGGGCACCTGATCGGCGGGAATAAACGCCACGCACTCTTGCGGTGCAAGCGTAACCTGCAAC‑GGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGGGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAat                                                                                                                                                                 <  1:221791/251‑1 (MQ=255)                                                                                                     ggaagaaacgcctcggaagcgggCGGTGAAAGCGAAACCGGAAAC‑GGCCCGTGGGCCGTCGCAAGCCAGAAGGAGACTTCTGTCCCTTGGGGGGGGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGa                                                                                                                                       <  1:74702/229‑1 (MQ=255)                                                                                                           aaCGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAAC‑GGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAgtgt                                                                                                                                 >  1:114695/1‑251 (MQ=255)                                                                                                                cACGGACTCTTGCGGTGCAAGCGTACCCT‑CAACCGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTAtt                                                                                                                            <  2:103013/251‑2 (MQ=255)                                                                                                                 ccGGACTCTTGCGGTGCAAGCGTAACCTGCAAC‑GGCCCGTTTGCCTGCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGGTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTAttc                                                                                                                           <  2:145142/250‑3 (MQ=255)                                                                                                                                 cAAGCGTAACCTGCAAC‑GGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGGTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCCTGCTTACGCAGGTAGTGTTTATTCAGCAGCGTTtgctgcc                                                                                                           >  2:80470/1‑250 (MQ=255)                                                                                                                                     cGTAACCTGCAAC‑GGCCCGTTGTCCGTCGCCCGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCCTGCTTACGCAGATTGTGTTTATTAAGCAGCGTTTGCTGCATATc                                                                                                       >  2:49234/1‑251 (MQ=255)                                                                                                                                                aC‑GGCCCGTGGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTgcg                                                                                            <  1:222919/251‑1 (MQ=255)                                                                                                                                                               gTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTTTACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACgg                                                                              >  2:11062/1‑251 (MQ=255)                                                                                                                                                                         agAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGGGGTTTTATACAGTCCTTACTGCCCGTAATATTCCTTCTCGCCATTCTTACGCAGATTGTGGTTATTCAGCAGCGTTTTCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATcc                                                                    >  2:5443/1‑251 (MQ=255)                                                                                                                                                                                      tGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGAc                                                       >  1:115602/1‑251 (MQ=255)                                                                                                                                                                                                  ggTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCCTCCAAAAACCAGGCTTGATTATAGCCTGGTTTCGGTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGAGCCATGCTTAAGCAGATAGTGTTTATTCAGCAGCGGTTGCTGCATATCCGGTAACAGCGGGGCTAACTGACGGCAGAATATCCCCAAATAAGCGACCTCTTCCAGCAc                                           >  2:92080/1‑251 (MQ=255)                                                                                                                                                                                                      tCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATg                                       >  1:259639/1‑251 (MQ=255)                                                                                                                                                                                                       cccGCCAGTGTCGGGGTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGGTTCGTTTGATTGGCTGGGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCCTGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGGAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATgg                                      >  2:235983/1‑251 (MQ=255)                                                                                                                                                                                                            cAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCCCGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATACCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGtt                                  >  1:22638/1‑250 (MQ=255)                                                                                                                                                                                                                              aaaCATGCCTGCGCCACGCTGTAATTCCATCAAATACCCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGGGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGc                >  2:116413/1‑250 (MQ=255)                                                                                                                                                                                                                                         cgcCAAGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGcccat    >  2:212244/1‑249 (MQ=255)                                                                                                                                                                                                                                            cACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGcccatgc  >  2:183915/1‑246 (MQ=255)                                                                                                                                                                                                                                            cACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGcccatgc  >  1:208717/1‑246 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                ATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAAC‑GGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATG  >  NC_000913/65544‑66027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑