Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,176,126	C→A	E66* (GAA→TAA)	gatZ ←	putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,176,126	0	C	A	95.8%	78.2 / ‑2.9	24	E66* (GAA→TAA)	gatZ	putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (7/16);  total (7/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.51e-01

CAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTATGC  >  NC_000913/2175886‑2176355                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                      cagcTCGTGCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCAGCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCGAGATGATCGCCGCCGAGAATAATGCGGTCGCGTGCAAACCCAGCTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCt                                                                                                                                                                                                                             <  1:211527/251‑1 (MQ=255)   gcTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTGTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGc                                                                                                                                                                                                                           <  2:307838/251‑1 (MQ=255)       gTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGgt                                                                                                                                                                                                                       >  1:246259/1‑251 (MQ=255)          ccGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGTc                                                                                                                                                                                                                    <  1:158552/251‑1 (MQ=255)          ccGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGTc                                                                                                                                                                                                                    <  2:304184/251‑1 (MQ=255)               aCCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTcc                                                                                                                                                                                                               <  2:34249/251‑1 (MQ=255)                                                                       aTGAATTTTACTGAAGCCGGCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTgc                                                                                                                                                       <  2:134465/251‑1 (MQ=255)                                                                                               aaCATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGcc                                                                                                                                >  1:203606/1‑250 (MQ=255)                                                                                                  atatTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTGGCTGCCAGCAGTTTGGCCCCAGATGAGCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAgcg                                                                                                                            <  2:244635/251‑1 (MQ=255)                                                                                                            aCCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGAt                                                                                                                  >  2:96671/1‑251 (MQ=255)                                                                                                                                   cgccgcATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGACGCCGAGAATACTGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAAc                                                                                              >  2:43198/1‑248 (MQ=255)                                                                                                                                             gcatTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACatat                                                                                 <  1:76273/251‑1 (MQ=255)                                                                                                                                                      tgctgcCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGat                                                                           <  1:248595/248‑1 (MQ=255)                                                                                                                                                       gctgcCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGt                                                                       <  1:163569/251‑1 (MQ=255)                                                                                                                                                                   ttGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAAACCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCttt                                                            >  1:40579/1‑250 (MQ=255)                                                                                                                                                                              ggatCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAAt                                                <  1:299272/250‑1 (MQ=255)                                                                                                                                                                                     cgccgAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGtt                                         <  1:242179/251‑1 (MQ=255)                                                                                                                                                                                         gagaATAAGGCGTTCGCGTGCAAACCCAACTTTAGCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCa                                     <  2:189448/251‑1 (MQ=255)                                                                                                                                                                                                atGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTAGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTCTGCCGGGCAATTAACGTTTTCATGttatt                              >  1:12229/1‑248 (MQ=255)                                                                                                                                                                                                atGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTGCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGttttt                              <  2:298851/251‑1 (MQ=255)                                                                                                                                                                                                       cgcgTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGt                       <  1:161820/251‑1 (MQ=255)                                                                                                                                                                                                        gcgTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTGATGCCGGGCAATTAACGTTGTCATGTTTTTTCCTTGtt                      <  2:31585/251‑1 (MQ=255)                                                                                                                                                                                                                         tATCGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGtt      <  2:256429/250‑1 (MQ=255)                                                                                                                                                                                                                            cGGCAATCGTAAAAACAAATTAGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTAtgc  >  2:242875/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                      CAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTATGC  >  NC_000913/2175886‑2176355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑