Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,594	A→C	F63V (TTT→GTT)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,594	0	A	C	95.8%	73.4 / NA	24	F63V (TTT→GTT)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (8/15);  total (9/15)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGC‑TGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTT  >  NC_000913/65349‑65828                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            gggATGCGGTTTCAGACGGGCATTAACGCTAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACCTCGGCCTAGTAGACGGTAACGCCAACTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACCCCGGCTGGCGGTGGAAATCCTTTAACGCCCGCGGGGTCCGGCGACAGccgt                                                                                                                                                                                                                                        >  1:320538/1‑249 (MQ=255)             cAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGc                                                                                                                                                                                                                             <  1:170992/250‑1 (MQ=255)                          aCGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGC‑TGAgc                                                                                                                                                                                                              <  1:71398/251‑1 (MQ=255)                                   ccATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACACCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGC‑TGAGCGCGGGGAAc                                                                                                                                                                                                     <  2:132935/251‑1 (MQ=255)                                          gCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGC‑TGAGCGCGGGGAACCTGATcg                                                                                                                                                                                              <  1:140334/251‑1 (MQ=255)                                                   ccTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGGGAAAATCCTTTAACGCCAGCGGGGTCAGGCGAACGCCTTGTTAACCCCGCAAAATATGC‑TGAGCGCGGGGAACCTGAACGGCGGGaaa                                                                                                                                                                                      >  1:253528/1‑249 (MQ=255)                                                        aCCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGGAGACGGTAACGCCCCCTTCACGCAGGCGCTTTTCGGAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGGAAATCCTTTAACGCCAGCGGGGTCACGAGAACGCCATGTTCCCCCCTCCAAATATGCCTG‑GCCCGGGGAACCttaactgcggggcttaaacc                                                                                                                                                                                >  2:137199/1‑232 (MQ=255)                                                            acacCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCCCGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGGAAATCCTTTAACGCCAGCGGGGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGC‑TGAGCGCGGGGGACCTGATCGGCggggcaaaaacccccc                                                                                                                                                                            >  2:248142/1‑238 (MQ=255)                                                                                           aTAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGC‑TGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTTCAAGCGTAACCTGCAACg                                                                                                                                             <  1:260481/251‑1 (MQ=255)                                                                                                                aTCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGC‑TGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGGGCAAGCGTAACCTTCAACGGCCCGTTGGCCGTcgcccgcc                                                                                                                        >  1:123073/1‑251 (MQ=255)                                                                                                                    gCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGC‑TGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGaa                                                                                                                    <  1:179694/251‑1 (MQ=255)                                                                                                                                        aCCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGC‑TGAGCGCGGGGAACCTGATCGGCGGGAAAAAAAGCCACCGACTCTTGCGGTGCAAGCGTAACCCGCCACGGGCCCTTGTCCCTCCCCAGCCAGAAggggaactccgtccccttcg                                                                                                >  1:195885/1‑233 (MQ=255)                                                                                                                                              aCGCAGGCGTTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGC‑TGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGTCCGTTGTCCGTTGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGgtgt                                                                                          <  1:285818/251‑1 (MQ=255)                                                                                                                                                    gcgcTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGC‑TGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGCCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGcc                                                                                    <  1:233411/251‑1 (MQ=255)                                                                                                                                                          ttCGTAATTCATCAATTGGCGATGGGCGAGACAGAAAAGGCCATACCCCGGCTGGCGGGGAAAATCCTTAACCGCCGGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGC‑TGAGGGCGGGGAACCTGATCGGCGGGAATAAACGCCCCGGACTCTTGCGGTGCAAGCGTAACCGGAAACGGCCCGTTGTCCGTCGCCAGCCAAAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAgtgt                                                                               <  2:250208/250‑1 (MQ=255)                                                                                                                                                           tCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGC‑TGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCgccagcca                                                                                                                       <  2:195779/209‑1 (MQ=255)                                                                                                                                                           tCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGATGGCGGTGAAACTCCTTTAACGAAAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGC‑TGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCAAGGAATCTTGCGGGGCAAGAGGAAACAGCCACAGACCGGTGTCCGTCGCCCGACAGAAGGAGAAATCTGTCCCCTGCGGGGGGTTCCGCCAgtgcgg                                                                             >  1:147216/1‑248 (MQ=255)                                                                                                                                                                   catcaATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGC‑TGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGa                                                                     <  1:311485/251‑1 (MQ=255)                                                                                                                                                                       aaTTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGC‑TGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATaaa                                                                 <  1:119944/251‑1 (MQ=255)                                                                                                                                                                              gaggggCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGC‑TGAGCGCGGGGAACCTGCTCGGCGGGAATAAAAGCCCCCGGCCCTTGCGGTGCCAGGGGAAACCGCGAAGGCCCCTTGTCCGTCCCCCGCCCGAAGGAGGATTCTTTCCCCTTCGGGGGGTCCCGCCAgtgacgcggtgtaaataaaactccc                                                          >  2:63711/4‑229 (MQ=255)                                                                                                                                                                                                    aTACACCGGCTTGCGCTGAAACCCCTTTAACGCCAGCTGTTTCAGGCGAACTCCTTGTTCCCCCTGCAAAATATGC‑TGCGCGCGGGTCCCCTGAGCGGCGGGAATAACCGCCACGGTCGCTTCCGGTGCAAGCGTACCCTGCAACGCCCCTTTGTCCTTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCTGGTTGTCCCGCCAGTGTCTTTTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATc                                    <  1:178533/251‑1 (MQ=255)                                                                                                                                                                                                     tACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGC‑TGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCCTGCGGGGGGTCCCGCCCGGGTCGGGGTAAAATAAAAACCGCCCGCGCCCCCCTGTAATTCCATc                                    >  1:87533/1‑250 (MQ=255)                                                                                                                                                                                                          ggggtGGCGGTGAAAATCCTTTAACGCCAGCGTTGTCAGGCGAACGCATTGTTCACCCTGCAAAAGATTC‑GGAGCGGGGGGAACCTGATGGGGGGAAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCCCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGGCGGGTTAAGATAAAACCTGCCTGCGCCTCGCTGTAATTCCATCaaaaaa                              <  2:101126/247‑1 (MQ=255)                                                                                                                                                                                                                                      aGCGGTGTCAGGCGAGCGCCCTGTTCCCCCTACAAAATATGC‑TGGGCGCGGGGACCCTGATGGGCGGTAATAAACGCCACGGACTCTGGCGGTGCAAGCGTAACCTGCACCGGCCCGTTGTCCGTCGCCACCCAGAAGGAGACTTCGGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGtt  <  2:118799/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            GGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGC‑TGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTT  >  NC_000913/65349‑65828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑