Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	968,837	G→A	D158N (GAT→AAT)	lpxK →	tetraacyldisaccharide 4'‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	968,837	0	G	A	100.0%	107.3 / NA	33	D158N (GAT→AAT)	lpxK	tetraacyldisaccharide 4'‑kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/22);  total (11/22)

CGCGTGGGGGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATT‑CTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTGATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTG  >  NC_000913/968597‑969064                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                    cgcgTGGGGGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATT‑CTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGaaa                                                                                                                                                                                                                            <  1:13226/250‑1 (MQ=255) cgcgTGGGGGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATT‑CTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAAt                                                                                                                                                                                                                           <  1:78734/251‑1 (MQ=255)    gTGGGGGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGGGATGAACCTGTGTTGATTTATTAACGCACTGATGCGCCTGTTGCGGTTTTTCCCGTTCTTTCTGATGCGGTAAAAGCCCTT‑TTTGCGCAACACCCTGATGTTCAGATAATCGTAACCGACGACGGTTTACAGACTTACCGTCTGGCGGGTAATGTgggaaatgt                                                                                                                                                                                                                        >  1:37506/1‑244 (MQ=255)         ggTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATT‑CTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTa                                                                                                                                                                                                                   <  2:234362/251‑1 (MQ=255)          gTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGGGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTTTCCCGTTCGTTCTGATGCGGGAAAACCCCTT‑CTGGCGCAACACCCTGATGTGCAGATCCTCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGGAAtttgcgtttt                                                                                                                                                                                                                  >  1:255668/1‑243 (MQ=255)            cGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATTAAAGCACCGATGCGCCTGTTGCGGGTTTTCCCCTTCGTTCTTATGCGGTAAAAGCCATTTCTGG‑GCCACCCCCCGATGTGCCGATCATCGTAAACCACGACGGGTTACAGCACTACCCTCTGGCGCGTAATGTGGAAATTgttgtttttt                                                                                                                                                                                                                >  2:99759/1‑247 (MQ=255)               aTCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATT‑CTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATg                                                                                                                                                                                                             <  1:218459/251‑1 (MQ=255)               aTCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGGTTCTCCCGGTCGTTCTGATGCGGTAAAAGCCATT‑CTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATg                                                                                                                                                                                                             >  1:135855/1‑251 (MQ=255)                      ggATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATTAACGCCCTGATGCGCCTGTTGCGGGTTCTCCCGTTTGTTTTGATTCGGGAAAAGCCCTT‑CTTGCGCAACACCCTGATGTGCAGATCATCGTAAACGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGGAAATGTCGGTTTTTATGGGGTGCg                                                                                                                                                                                                      >  2:249572/1‑251 (MQ=255)                                gtAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGGGATGAACCTGTGCTGATTTTTCAAAGCACTGATGCCCCCGTTGGGGTTTCTCCCGTTCGTTCTTATGCGGCAAAAGCCATT‑CTGGCGCCACACCCTCATGTGCAGATCATTGTAACCGACGACGGTTTACAACATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTggg                                                                                                                                                                                            >  1:205047/1‑250 (MQ=255)                                                        tATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTTATTTATCAACGCCCTGATGGGCCTGGTGCGGGTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATT‑CTGGCGCAACAACCTGATGTGCAGATCATCGTAACCGACGGCGGTTTAAAGCCTTACCGTCTGGCGCGTAATGTGGAAAATGTCGTTATTTTTTGGGTGTGTCGCTTTGGCAATGGCTGGGGGGTGCCGgggggg                                                                                                                                                                    >  1:249165/1‑251 (MQ=255)                                                         aTTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATGTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTGCGTTCTGATGCGGTAAAAGCCATT‑CTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTGGGCAATGGCTGGTGGTTGCCGGCgggg                                                                                                                                                                    <  1:109948/250‑1 (MQ=255)                                                                      aCCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATT‑CTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGTGGCCAATGCGTgagcg                                                                                                                                                      <  2:118586/251‑1 (MQ=255)                                                                                       gggTGAGGAAACTGTGTTGATTTATCGACGCACTGATGCGCCTGTTGCGGTTTCTCGCGTTCGTTCTGATGCGGTAAAAGCCATT‑CTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGGTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGGACGTGGGGCTTAAAGt                                                                                                                                     <  2:312621/251‑1 (MQ=255)                                                                                                    gtgtTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATT‑CTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCgg                                                                                                                         <  1:267224/250‑1 (MQ=255)                                                                                                    gtgtTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATT‑CTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTACCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCg                                                                                                                          <  1:221599/249‑1 (MQ=255)                                                                                                      gtTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATT‑CTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTGGATGCGGTaa                                                                                                                      <  1:171265/251‑1 (MQ=255)                                                                                                         gATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATT‑CTGGCGCAACACCCTGATGTGCAGATCATCGTAAACGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAACTTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCTATTCGGGAGCGAGCGGGGGGCTTAAAGGTGGGTGAGGCGGTAATCg                                                                                                                   >  2:152535/1‑251 (MQ=255)                                                                                                                  aCGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATT‑CTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAAcggcg                                                                                                          <  2:135855/251‑1 (MQ=255)                                                                                                                    gCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATT‑CTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCGGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGgt                                                                                                        <  1:143319/251‑1 (MQ=255)                                                                                                                     cACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATT‑CTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCGGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGgtg                                                                                                       <  1:284984/251‑1 (MQ=255)                                                                                                                        tGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATT‑CTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCCATGCGTGGGCGGGCGGGGGGCTTAAAGTCGGGTGATGCGGTAATCGGCACCGGCGgtgggc                                                                                                    >  2:199830/1‑248 (MQ=255)                                                                                                                            gcgcCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATT‑CTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCg                                                                                                <  1:68780/251‑1 (MQ=255)                                                                                                                                  gTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATT‑CTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCg                                                                                           <  1:103057/250‑1 (MQ=255)                                                                                                                                                cgttcgttCTGATGCGGTAAAAGCCATT‑CTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAAGCCCCATGc                                                                            <  1:267216/251‑1 (MQ=255)                                                                                                                                                          gATGCGGTAAAAGCCATT‑CTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGTCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCCTGCATctgct                                                                     <  2:170654/248‑1 (MQ=255)                                                                                                                                                               ggTAAAAGCCATT‑CTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAAGCGTCAACGGCGGTGTCCCTCTCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTc                                                             <  1:268526/251‑1 (MQ=255)                                                                                                                                                                      gCCATT‑CTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAAGGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGTAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGt                                                      <  1:44635/251‑1 (MQ=255)                                                                                                                                                                                                   ggATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGAc                          <  2:68885/250‑1 (MQ=255)                                                                                                                                                                                                    gATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGGGGTTGCCGGCGGGGCCAATGCGGGAGCGAGCGGGGCGCTTAAAGGCGGGTGATGCGGGAATTGTCAACGGCGGGGGGCCCCGCAGCGGGGAAATCCCCCTGCAACTGCTGCCGGGTCCGGCGGGGGATTTaaggaacgggaagcggtggggcg                         >  1:246400/1‑229 (MQ=255)                                                                                                                                                                                                     atcatcGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGGGCTTAAAGTCGGGTGATGCGGGAATCGTCAACGGCGGGGTCCCTCGCCGCGGGGGAAACCCCCTTCATCTGCCGCCGGGTCAGGCGGGGGATTTACGTAACGGGACGCGGtgtggagt                        >  1:206175/1‑247 (MQ=255)                                                                                                                                                                                                     atcatcGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGt                        <  2:184188/251‑1 (MQ=255)                                                                                                                                                                                                                            aCAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTg  <  1:103264/250‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                    CGCGTGGGGGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATT‑CTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTGATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTG  >  NC_000913/968597‑969064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑