Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 246,747 G→T S12S (TCG→TCT)  yafL → NlpC/P60 family protein YafL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913246,7470GT96.7% 92.8 / NA 30S12S (TCG→TCT) yafLNlpC/P60 family protein YafL
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (16/13);  total (16/14)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTT‑GTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGG  >  NC_000913/246506‑246975
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                    
ggTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTT‑GTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTg                                                                                                                                                                                                                             >  1:147252/1‑251 (MQ=255)
           ggCTGTATATTGATTCAGCTTGAATTGTAGCTCAAATGAGCAAAAGCGACAATCCATGAGTAATTTAATAAAAGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGTTTTATTAAGTTT‑GTTCAGAAACGATCCGGGATACTCTTCAACATTAAAGCATGACCAGCCATTATTTAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGAtttgttt                                                                                                                                                                                                                  <  2:110278/250‑1 (MQ=255)
              tGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGCAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTT‑GTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGcc                                                                                                                                                                                                               >  2:198106/1‑251 (MQ=255)
                   atttattCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTT‑GTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGtttt                                                                                                                                                                                                          >  2:28712/1‑251 (MQ=255)
                    tttattCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTT‑GTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTttc                                                                                                                                                                                                         >  1:100208/1‑251 (MQ=255)
                                                gAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTT‑GTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACcacatcacag                                                                                                                                                                             <  2:262664/251‑2 (MQ=255)
                                                           aCAATCCATGCGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTT‑GTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGACATTATTGAGTATGTCCTTGCCGTCGATTCAATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCa                                                                                                                                                                   >  2:200417/1‑250 (MQ=255)
                                                                                                     tAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTT‑GTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTttatt                                                                                                                        >  2:98866/1‑251 (MQ=255)
                                                                                                                      ttAATTTTATAATAATGTTTTAT‑AAGTTTTGTTCAAAAAGTATCGGGGATCCATTGCAACATTAAATCTTGACCAGCCATTATTGAGTATGTCCTTGCCTTCGATTCCTTCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCCCCCCATCACATATCTCTTTCAGCAACCCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACt                                                                                                        <  2:13245/250‑1 (MQ=255)
                                                                                                                       tAATTTTATAATAATGGTTTATTAAGTTT‑GTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTc                                                                                                       >  2:215357/1‑250 (MQ=255)
                                                                                                                           tttATAATAATGGTTTATTAAGTTT‑GTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTg                                                                                                  >  2:64561/1‑251 (MQ=255)
                                                                                                                             tataATAATGGTTTATTAAGTTT‑GTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGa                                                                                                 >  1:56964/1‑250 (MQ=255)
                                                                                                                               aaaaaatGTTTTATTAATTTT‑TTTAAGAAACGTTCCGGGATCCACAGCAACATTAAAACATGACCATCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTCCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGaaaa                                                                                              <  2:2828/247‑1 (MQ=255)
                                                                                                                                aataatGGTTTATTAAGTTT‑GTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTCGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGaaaa                                                                                              >  2:215755/1‑250 (MQ=255)
                                                                                                                                aagaatGGTTTATTAAGTTT‑GTTCAGAAACGATCCGGGATACACTGAAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCCCCACATCCCATATCTCTTTCAGTTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGaaaaa                                                                                             <  1:177724/248‑1 (MQ=255)
                                                                                                                                       gTTTATTAAGTTT‑GTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGcc                                                                                      <  1:28712/251‑1 (MQ=255)
                                                                                                                                          tattaAGTTT‑GTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGc                                                                                   >  2:2786/1‑251 (MQ=255)
                                                                                                                                            ttaAGTGT‑GTTCAAAAACGATCCGGGATACAAGGCACCATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTGGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCt                                                                                  <  2:265561/250‑1 (MQ=255)
                                                                                                                                                                              ccAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGgc                                                <  1:78690/250‑1 (MQ=255)
                                                                                                                                                                                     tAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGc                                         >  2:258397/1‑251 (MQ=255)
                                                                                                                                                                                        agatttaCCAGCCATTATTGAGTTTGTCCTTGCGGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTTTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAg                                      <  2:228653/245‑1 (MQ=255)
                                                                                                                                                                                               ccAGCCATTATTCAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGACAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAAcc                               >  2:63776/1‑251 (MQ=255)
                                                                                                                                                                                                 aGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCgg                             <  1:264135/251‑1 (MQ=255)
                                                                                                                                                                                                  gCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCTTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCggg                            <  1:194107/251‑1 (MQ=255)
                                                                                                                                                                                                     attattGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGc                         >  1:243396/1‑251 (MQ=255)
                                                                                                                                                                                                          ggggTATGTCTTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGAt                     <  2:195777/247‑1 (MQ=255)
                                                                                                                                                                                                              tATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAAc                >  2:13764/1‑251 (MQ=255)
                                                                                                                                                                                                                tGTCCTTTCCGTCGATTCCATCCTTTTTTTTGTCGGGATTACTGTTGATTTTTTTGCCGTTTTCTTCATTTGCCAGCGCCCCCACATCCCATTTCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACa               <  2:321039/250‑1 (MQ=255)
                                                                                                                                                                                                                      ggctgTCGATCCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTGTCCGTTTTCTTCTTTTGCCCGCGCCACCACATCCCATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATcc         <  2:243004/246‑1 (MQ=255)
                                                                                                                                                                                                                            cGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCCTTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTCTTAAAACAGTTCCACACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGATAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGACTgg  >  2:174251/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                    
GGTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTT‑GTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGG  >  NC_000913/246506‑246975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑