Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	92,354	A→G	V314V (GTA→GTG)	ftsI →	peptidoglycan DD‑transpeptidase FtsI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	92,354	0	A	G	100.0%	149.5 / NA	43	V314V (GTA→GTG)	ftsI	peptidoglycan DD‑transpeptidase FtsI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/27);  total (16/27)

GGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGG  >  NC_000913/92108‑92586                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         ggCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATg                                                                                                                                                                                                                                       <  1:124564/250‑1 (MQ=255)  gcgcTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGAc                                                                                                                                                                                                                                     >  1:104143/1‑251 (MQ=255)    gcTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACcg                                                                                                                                                                                                                                   <  1:35071/251‑1 (MQ=255)               atgaACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAAcgt                                                                                                                                                                                                                        <  1:220390/251‑1 (MQ=255)                     gCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAAcgtggcgtg                                                                                                                                                                                                                  <  1:254185/251‑1 (MQ=255)                         gCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCgtggtg                                                                                                                                                                                                               <  1:113038/250‑1 (MQ=255)                              cgcTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGaa                                                                                                                                                                                                         <  2:166589/251‑1 (MQ=255)                                         cgcgAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACt                                                                                                                                                                                              <  2:79237/251‑1 (MQ=255)                                           cgAACTGAACAATGCGGTGTCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCATCTCCGGTGAAGTGCTGTCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCTCTCCGAAAGAGGCGATGCGTATCCGTACCATCACCGACGTGTTTGAACCGGGCTCAACGGTGAAACCGATGGTAGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCa                                                                                                                                                                                            <  1:317456/251‑1 (MQ=255)                                           cgAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCa                                                                                                                                                                                            <  1:80255/251‑1 (MQ=255)                                                              gccgttAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCGGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAAt                                                                                                                                                                         <  2:239314/247‑1 (MQ=255)                                                                   tAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAAc                                                                                                                                                                     <  1:158753/250‑1 (MQ=255)                                                                   tAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACg                                                                                                                                                                    >  1:35911/1‑251 (MQ=255)                                                                     aCAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGc                                                                                                                                                                  <  2:300021/251‑1 (MQ=255)                                                                           cTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGa                                                                                                                                                             >  2:88935/1‑250 (MQ=255)                                                                                      aGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGt                                                                                                                                                 <  1:73382/251‑1 (MQ=255)                                                                                            gTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACg                                                                                                                                           <  1:278781/251‑1 (MQ=255)                                                                                                tggtggATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTAc                                                                                                                                       >  1:103732/1‑251 (MQ=255)                                                                                                    ggATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGc                                                                                                                                    <  2:107558/250‑1 (MQ=255)                                                                                                                cGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGa                                                                                                                       <  2:71353/251‑1 (MQ=255)                                                                                                                                   gCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGCTGCGTACCCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTc                                                                                                    >  1:214130/1‑251 (MQ=255)                                                                                                                                         aGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTaa                                                                                              <  2:188237/251‑1 (MQ=255)                                                                                                                                           cccGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACg                                                                                            <  2:35911/251‑1 (MQ=255)                                                                                                                                            ccGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACgg                                                                                           >  2:61415/1‑250 (MQ=255)                                                                                                                                                cATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCgg                                                                                        <  2:12404/250‑1 (MQ=255)                                                                                                                                                 aTACAACCCTAACAATCTGAGCGGCACGACGAAAGAGGCGAAGCGTAACCGAACCATCCCCGACGTGTTTGAACCTAGCTCAACGGTTAAACCGATGGTGGCGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAACACTCGGTACTCAATACCATTCCTTATCGACTTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCggcg                                                                                      >  1:302326/1‑249 (MQ=255)                                                                                                                                                           aaCAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTCACCGTACCATCACCGACGTGTTTGCACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCCTTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCt                                                                            >  2:18526/1‑251 (MQ=255)                                                                                                                                                             cAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTGAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTgg                                                                          >  2:153147/1‑251 (MQ=255)                                                                                                                                                                      cGGCACGCCGAAAGCGGCGTTGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGCCCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTACTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGCATTACAGAAGTCGAGTAACGTCGGTGTTTCCCAGCTGGCGTTAGCgc                                                                 >  2:134127/1‑250 (MQ=255)                                                                                                                                                                               gAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCCACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTG‑CACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTc                                                       >  1:39567/1‑251 (MQ=255)                                                                                                                                                                                 aaGAGGCGATGCGTAACCGTACCATCACCGACGCGTTTGAACATGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCCACGTGGCGTGGTGCGGGAAAACTCGGTACTCACTACCATTCCTTATCGAATTAACAGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTc                                                       >  1:141129/1‑250 (MQ=255)                                                                                                                                                                                       cGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTta                                                <  1:226060/251‑1 (MQ=255)                                                                                                                                                                                        gATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTtag                                               <  2:195114/251‑1 (MQ=255)                                                                                                                                                                                             gTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCCCGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTtagt                                              >  2:68018/1‑247 (MQ=255)                                                                                                                                                                                                   gTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGAtacttac                                    <  1:107200/251‑1 (MQ=255)                                                                                                                                                                                                       cATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCAc                                <  1:22373/251‑1 (MQ=255)                                                                                                                                                                                                        aTCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACg                               >  1:88931/1‑251 (MQ=255)                                                                                                                                                                                                            ccGACGCGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGtttt                           >  1:244444/1‑251 (MQ=255)                                                                                                                                                                                                                   gtTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTgg                    <  2:104143/251‑1 (MQ=255)                                                                                                                                                                                                                       gAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTTCGGGAAAACTCGTTACTCAATACCATTCCTTATCGAAGTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTACCCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCATCGTTAGTAGATACTTACTCACGTTTTGGACTTGGaaa                <  2:114063/251‑1 (MQ=255)                                                                                                                                                                                                                             ggCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGAc          <  2:103732/251‑1 (MQ=255)                                                                                                                                                                                                                                   aCGGTTTAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAAttt    >  2:83492/1‑251 (MQ=255)                                                                                                                                                                                                                                     ggTTAAACAGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGACCTCATCGTTAGTAGATACTTACTCACGTTTTGGACTGGGaaaatccaccattttat  <  2:6759/251‑14 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         GGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGG  >  NC_000913/92108‑92586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑