Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	92,162	C→T	N250N (AAC→AAT)	ftsI →	peptidoglycan DD‑transpeptidase FtsI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	92,162	0	C	T	100.0%	168.7 / NA	51	N250N (AAC→AAT)	ftsI	peptidoglycan DD‑transpeptidase FtsI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/25);  total (26/25)

GTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCA  >  NC_000913/91922‑92400                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               atccgGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCt                                                                                                                                                                                                                                      <  1:66194/250‑1 (MQ=255)     ggCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTa                                                                                                                                                                                                                                   >  1:188237/1‑250 (MQ=255)            tGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCt                                                                                                                                                                                                                           >  1:3769/1‑251 (MQ=255)             gACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTg                                                                                                                                                                                                                          >  1:231725/1‑251 (MQ=255)              aCTGCTCACCTCATTGGCTTTACTCACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAACTGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTg                                                                                                                                                                                                                          >  2:73382/1‑250 (MQ=255)                 gCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATc                                                                                                                                                                                                                      >  1:26782/1‑251 (MQ=255)                      ccTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGt                                                                                                                                                                                                                  >  1:239314/1‑250 (MQ=255)                        tCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGACTCTGGTAgc                                                                                                                                                                                                               >  1:284474/1‑251 (MQ=255)                                   aCTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCtggt                                                                                                                                                                                                    >  1:271547/1‑251 (MQ=255)                                               aGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAacac                                                                                                                                                                                        <  2:201954/251‑1 (MQ=255)                                               aGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATGGGGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTACGCACAACCTGGCGCTGAGTATTGGTGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGTCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAaca                                                                                                                                                                                         <  1:179451/250‑1 (MQ=255)                                                   aaGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCggg                                                                                                                                                                                    >  2:27839/1‑250 (MQ=255)                                                              ggCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGc                                                                                                                                                                         <  2:90627/251‑1 (MQ=255)                                                                     agaagaGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCt                                                                                                                                                                  >  2:228588/1‑251 (MQ=255)                                                                       aagaGTGTCGATAAATGGCGTACCGGGCAGCCGGGTGAGCGCCTTGTGCGTAAAGACAGCTATGGTCGCGTAAGTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGGACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTat                                                                                                                                                                <  2:12876/251‑2 (MQ=255)                                                                         gagTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACa                                                                                                                                                              <  2:80036/251‑1 (MQ=255)                                                                              tCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGccc                                                                                                                                                          <  1:32101/250‑1 (MQ=255)                                                                                   aaaTGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCAt                                                                                                                                                     >  1:14372/1‑250 (MQ=255)                                                                                    actGGCTTACCGTTCAGCCGGGTGAGCGCATTGTTCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGTTGGCCTTTCACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTACCAGCCCGTCATAc                                                                                                                                                   >  2:104612/3‑251 (MQ=255)                                                                                            aCCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTa                                                                                                                                            <  1:122976/250‑1 (MQ=255)                                                                                              cGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAAc                                                                                                                                          >  2:124564/1‑250 (MQ=255)                                                                                                            aGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACg                                                                                                                           <  1:27839/251‑1 (MQ=255)                                                                                                                  ttGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGaa                                                                                                                      >  1:71353/1‑250 (MQ=255)                                                                                                                    gtgCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAag                                                                                                                    <  1:314456/250‑1 (MQ=255)                                                                                                                       ggTAAAGAACGCTATTGTCGCGAAATTGACGGTATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGCTGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGAAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACCGCCCGTCATACAACCCTAACACTCAGAGCGGCACGCCGACagaagg                                                                                                                >  2:189439/2‑248 (MQ=255)                                                                                                                         tAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCg                                                                                                               >  1:268170/1‑250 (MQ=255)                                                                                                                                                 tGAAGATATTTCTTCTAGTGACAGCAAGGCTGCTCACAACCTGGCGCTGAGTATTGATGACCGCCTGCAGGCGCTGGGTTATCGCGAACTGAACAATGCGGTGGTCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGAGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAAAAGCAAGTAATACAACACTAACAATCTGAGAGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGAcc                                                                                      >  2:291560/1‑250 (MQ=255)                                                                                                                                                 tGAAGATATTTCTTCTACTTACAGCCAGGCATCGCACAACCTGGCGCTGAGTATTGATTAACGCCTGAAGGCGCTGGTTTATCGCGAACTGAAAAATGCGGTGACCTTTAACAAGGCTGAATCTGGCAGCGCCGTGCTGGTGGATCTCAACACCGGGGAAGTGATGGCGATGGCTAACAGCCCGTAATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTACCCGTACCATCACCGACg                                                                                      >  2:291554/1‑251 (MQ=255)                                                                                                                                                      atagatcttcgACTGACAGCCAGGCTGCGCACAACCTGGCGCGGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGATCAATGCGGTGGCCGTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCGGAGCGGCACGCCGAAAGAGGCGATGCTTAACCGTACCATCTCCGACGTGttt                                                                                 <  2:269287/246‑1 (MQ=255)                                                                                                                                                         tttCTTCTACTGCCAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGa                                                                               >  2:315878/1‑250 (MQ=255)                                                                                                                                                          ttcttcTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAAc                                                                             >  1:113716/1‑251 (MQ=255)                                                                                                                                                           tcttctACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAAcc                                                                            >  1:114765/1‑251 (MQ=255)                                                                                                                                                                 aCTGACAGCCAAGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTc                                                                      >  2:226060/1‑251 (MQ=255)                                                                                                                                                                    gACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTTTTTGAACCTGGCTCAAc                                                                   <  2:22652/251‑1 (MQ=255)                                                                                                                                                                        gccatgctgcgcactcccTGGCGCTTATTATTTATGAACGCCTGCAGGCTCTTGTTTATCGCGAACTGAACAATTCGGTGGCCTTTAACATGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACTCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGt                                                                <  2:26782/234‑1 (MQ=255)                                                                                                                                                                          cAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTa                                                              >  1:304786/1‑250 (MQ=255)                                                                                                                                                                           aGGCTGCGCACAAACTGTCGCTGAGTATTTATGAACGCCTGCTGGCGATGGTTTATAGCGAACTGAACAATGCGGTGGACTTTAAAAAGTATGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCGGGCGATGGCTAACAGCACGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTa                                                              <  1:235211/249‑1 (MQ=255)                                                                                                                                                                             gctgcgcACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTTAGCCGCACGCCGAAAGAGGCGATGCGTAACCGTGCCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAAcc                                                          <  1:291560/248‑1 (MQ=255)                                                                                                                                                                             gctgcgcACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAATAGGCGATTGGTAACCGTACCATCTCCTACGTGTTTGAACCTGGCTCAACGGTTAAAcc                                                          <  1:291554/248‑1 (MQ=255)                                                                                                                                                                                 cgcACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGAtg                                                      >  1:249322/1‑251 (MQ=255)                                                                                                                                                                                    acaACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGAtggtg                                                   >  1:4027/1‑251 (MQ=255)                                                                                                                                                                                           ggCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATg                                             <  1:124564/250‑1 (MQ=255)                                                                                                                                                                                            gcgcTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGAc                                           >  1:104143/1‑251 (MQ=255)                                                                                                                                                                                              gcTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACcg                                         <  1:35071/251‑1 (MQ=255)                                                                                                                                                                                                         atgaACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAAcgt                              <  1:220390/251‑1 (MQ=255)                                                                                                                                                                                                               gCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAAcgtggcgtg                        <  1:254185/251‑1 (MQ=255)                                                                                                                                                                                                                   gCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCgtggtg                     <  1:113038/250‑1 (MQ=255)                                                                                                                                                                                                                        cgcTGGTTTATCGCGAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGaa               <  2:166589/251‑1 (MQ=255)                                                                                                                                                                                                                                   cgcgAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACt    <  2:79237/251‑1 (MQ=255)                                                                                                                                                                                                                                     cgAACTGAACAATGCGGTGTCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCATCTCCGGTGAAGTGCTGTCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCTCTCCGAAAGAGGCGATGCGTATCCGTACCATCACCGACGTGTTTGAACCGGGCTCAACGGTGAAACCGATGGTAGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCa  <  1:317456/251‑1 (MQ=255)                                                                                                                                                                                                                                     cgAACTGAACAATGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCTGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCa  <  1:80255/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               GTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCA  >  NC_000913/91922‑92400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑